Protective effect of Berberis vulgaris on Fenton reaction-induced DNA cleavage

Document Type : Original Research Article

Authors

Department of Biology, Faculty of Science, Yazd University, Yazd, Iran

Abstract

Objective: Berberis vulgaris contains antioxidants that can inhibit DNA cleavage. The purpose of this study was to evaluate the antioxidant and protective activity of B. vulgaris on DNA cleavage.
Materials and Methods: In this study, the antioxidant capacity of B. vulgaris was investigated using DPPH and its protective effect was evaluated on pBR322 plasmid and lymphocyte genomic DNA cleavage induced by Fenton reaction, by DNA electrophoresis.
Results: Aqueous extract of B. vulgaris presented dual behavior with a potent antioxidant activity at 0.25and 0.75mg/ml for pBR322 plasmid and lymphocyte genomic DNA, respectively, but a pro-oxidant activity was observed at higher concentrations.
Conclusion: Our results indicated that B. vulgaris extract an inhibit Fenton reaction-induced DNA cleavage and oxidative cleavage of double-stranded DNA assay is a powerful technique that can be used to determine the antioxidant and pro-oxidant properties of a compound on cellular components such as DNA.

Keywords

Main Subjects


Abbas SR, Sabir SM, Ahmad SD, Boligon AA, Athayde ML. 2014. Phenolic profile, antioxidant potential and DNA damage protecting activity of sugarcane (Saccharum officinarum). Food Chem, 147: 10-16.
Adly AA. 2010. Oxidative stress and disease: an updated review. Res J Immunol, 3: 129-145.
Ajith T. 2010. Ameliorating reactive oxygen species-induced in vitro lipid peroxidation in brain, liver, mitochondria and DNA damage by Zingiber officinale Roscoe. Indian J Clin Biochem, 25: 67-73.
Bouayed J, Bohn T. 2010. Exogenous antioxidants--Double-edged swords in cellular redox state: health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxid Med Cell Longev, 3: 228-237.
Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol, 28: 25-30.
Chang CY, Hsieh YH, Cheng KY, Hsieh LL, Cheng TC, Yao KS. 2008. Effect of pH on Fenton process using estimation of hydroxyl radical with salicylic acid as trapping reagent. Water Sci Technol, 58: 873-879.
Charehsaz M, Sipahi H, Celep E, Üstündağ A, Ülker ÖC, Duydu Y, Aydın A, Yesilada E. 2015. The fruit extract of Berberis crataegina DC: exerts potent antioxidant activity and protects DNA integrity. DARU J Pharm Sci, 23: 1-7.
Engelmann MD, Bobier RT, Hiatt T, Cheng IF. 2003. Variability of the Fenton reaction characteristics of the EDTA, DTPA, and citrate complexes of iron. Biometals, 16: 519-527.
Ferretti G, Bacchetti T, Belleggia A, Neri D. 2010. Cherry antioxidants: from farm to table. Molecules, 15: 6993-7005.
Flemmig J, Arnhold J. 2007. Ferrous ion-induced strand breaks in the DNA plasmid pBR322 are not mediated by hydrogen peroxide. Eur Biophys J, 36: 377-384.
Goldstein S, Meyerstein D, Czapski G. 1993. The Fenton reagents. Free Radic Biol Med, 15: 435-445.
Halliwell B, Aruoma OI. 1991. DNA damage by oxygen‐derived species Its mechanism and measurement in mammalian systems. FEBS letters, 281: 9-19.
Henle ES, Linn S. 1997. Formation, prevention, and repair of DNA damage by iron/hydrogen peroxide. J Biol Chem, 272: 19095-19098.
Hoshyar R, Mahboob Z, Zarban A. 2016. The antioxidant and chemical properties of Berberis vulgaris and its cytotoxic effect on human breast carcinoma cells. Cytotechnology, 68: 1207-1213.
Huang X. 2003. Iron overload and its association with cancer risk in humans: evidence for iron as a carcinogenic metal. Mutat Res, 533: 153-171.
Imlay JA, Chin SM, Linn S. 1988. Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro. Science, 240: 640-642.
Joukar S, Mahdavi N. 2014. Alterations of blood pressure and ECG following two-week consumption of Berberis integerrima fruit extract. IntSch Res Notices, 2014: 1-6.
Jung YS, Lim WT, Park JY, Kim YH. 2009. Effect of pH on Fenton and Fenton‐like oxidation. Environ Technol, 30: 183-190.
Kitts DD, Wijewickreme AN, Hu C. 2000. Antioxidant properties of a North American ginseng extract. Mol Cell Biochem, 203: 1-10.
Kumar M, Kumar S, Kaur S. 2011. Investigations on DNA protective and antioxidant potential of chloroform and ethyl acetate fractions of Koelreuteria paniculata Laxm. Afr J Pharm Pharmacol, 5: 421-427.
Leba LJ, Brunschwig C, Saout M, Martial K, Vulcain E, Bereau D, Robinson JC. 2014. Optimization of a DNA nicking assay to evaluate Oenocarpus bataua and Camellia sinensis antioxidant capacity. Int J Mol Sci, 15: 18023-18039.
Mazloum-Ardakani M, Salehpour E, Heidari M, Zomorodipour A. 2013. The effect of pomegranate juice as a natural antioxidant to prevent DNA damages is detectable by application of electrochemical methods. Scientia Iranica, 20: 566-570.
Mohamadi M, Maskooki A, Mortazavi S. 2012. Evaluation of antioxidant properties of Barberry fruits extracts using maceration and subcritical water extraction (SWE). Int J Nutr Food Eng, 6: 699-703.
Ohashi Y, Yoshinaga K, Yoshioka H, Yoshioka H. 2002. Kinetic analysis of the effect of (-)-epigallocatechin gallate on the DNA scission induced by Fe(II). Biosci Biotechnol Biochem, 66: 770-776.
Orrenius S, Gogvadze V, Zhivotovsky B. 2007. Mitochondrial oxidative stress: implications for cell death. Annu Rev Pharmacol Toxicol, 47: 143-183.
Procházková D, Boušová I, Wilhelmová N. 2011. Antioxidant and prooxidant properties of flavonoids. Fitoterapia, 82: 513-523.
Rajendran P, Nandakumar N, Rengarajan T, Palaniswami R, Gnanadhas EN, Lakshminarasaiah U, Gopas J, Nishigaki I. 2014. Antioxidants and human diseases. Clin Chim Acta, 436: 332-347.
Ryan P, Hynes MJ. 2007. The kinetics and mechanisms of the complex formation and antioxidant behaviour of the polyphenols EGCg and ECG with iron(III). J Inorg Biochem, 101: 585-593.
Sakihama Y, Cohen MF, Grace SC, Yamasaki H. 2002. Plant phenolic antioxidant and prooxidant activities: phenolics-induced oxidative damage mediated by metals in plants. Toxicology, 177: 67-80.
Scheibmeir HD, Christensen K, Whitaker SH, Jegaethesan J, Clancy R, Pierce JD. 2005. A review of free radicals and antioxidants for critical care nurses. Intensive Crit Care Nurs, 21: 24-28.
Yildiz H, Ercisli S, Sengul M, Topdas EF, Beyhan O, Cakir O, Narmanlioglu HK, Orhan E. 2014. Some physicochemical characteristics, bioactive content and antioxidant characteristics of non-sprayed Barberry (Berberis vulgaris L.) fruits from Turkey. Erwerbs-Obstbau, 56: 123-129.
Zhao Y, Zhao B. 2013. Oxidative stress and the pathogenesis of Alzheimer's disease. Oxid Med Cell Longev, 2013: 316523.