In vitro and in vivo antifungal activity of Allium hirtifolium and Allium sativum

Document Type : Original Research Article

Authors

Department of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran

Abstract

Objective: One of the major clinical problems is the resistance of Candida speciestowards most of antifungals. The search for new antifungal drug from plants have helped to overcome this problem. This study evaluated the effects of Allium hirtifolium and Allium sativumextracts on Candida tropicalis both in vitro and in a mouse model of systemic candidiasis.
Materials and Methods: In this study, clinical isolates of Ctropicalis were isolated and identified from immunocompromised patients with recurrent candidiasis. Antifungal susceptibilities assessment and time kill study of aqueous and ethanolic extracts of A. hirtifolium and A. sativumextracts were done against Ctropicalis. The in vivo activity of aqueous extracts of A. hirtifolium and A. sativumwere evaluated in a mouse model of systemiccandidiasis caused by Ctropicalis through estimating the host survival time, fungal burden and histopathologic analyses.
Results: The aqueous and ethanolic extracts of A. hirtifolium and A. sativum exhibited significant antifungal activity against Ctropicalis. In time kill study, A. hirtifolium and A. sativum extracts exhibited significant effects against Ctropicalis (p<0.05). Treatment of BALB/c mice that were systemicallyinfected with C. tropicalis, showed that treatments with A. hirtifolium and A. sativum (at 1 mg/kg/day) were slightly less efficacious than that of fluconazole in terms of the fungal burden reduction and host survival time, it was still effective against C. tropicalis.
Conclusion: These findings demonstrate the anticandidal properties of A. hirtifolium and A. sativumextracts in vitro and in vivo and suggest their potential to be used as an adjuvant therapy in the management of Candida infections.

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Main Subjects


Alizadeh M, Kolecka A, Boekhout T, Zarrinfar H, Ghanbari Nahzag MA, Badiee P, Rezaei-Matehkolaei A, Fata A, Dolatabadi S, Najafzadeh MJ. 2017a. Identification of Candida species isolated from vulvovaginitis using matrix assisted laser desorption ionization-time of flight mass spectrometry. Curr Med Mycol, 3: 21–25. 
Alizadeh F, Khodavandi A, Zalakian S.2017b.Quantitation of ergosterol content and gene expression profile of ERG11 gene in fluconazole-resistant Candida albicans. Curr Med Mycol, 3: 13–19.
Antinori S, Milazzo L, Sollima S, Galli M, and Corbellino M. 2016. Candidemia and invasive candidiasis in adults: a narrative review. Eur J Intern Med, 34: 21-28.
Bayan L, Koulivand PH, Gorji A. 2014. Garlic: a review of potential therapeutic effects. Avicenna J Phytomed, 4: 1-14.
Fattorusso E, Iorizzi M, Lanzotti V, Taglialatela-Scafati O. 2002. Chemical composition of shallot (Allium ascalonicum Hort.). J Agric Food Chem, 50: 5686-5690.
Ghahremani-majd H, Dashti F, Dastan D, Mumivand H, Hadian J, Esna-Ashari M. 2012. Antioxidant and antimicrobial activities of Iranian mooseer (Alliumhirtifolium Boiss) populations. Hort Environ Biotechnol, 53: 116-122.
Ghannoum MA. 1988. Studies on the anticandidal mode of action of Allium sativum (garlic). J Gen Microbiol, 134: 2917-2924.
Katiraee F, Eidi S, Bahonar AR, Zarrinfar H, Khosravi AR. 2008. Comparison of MICs of some Iranian herbal essences against azole resistance and azole susceptible of Candida albicans. J Medic Plants, 7: 37-44.
Khodavandi A, Harmal NS, Alizadeh F, Scully OJ, Sidik SHM, Othman F, Sekawi Z, Ng KP, Chong PP. 2011a. Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression. Phytomedicine, 19: 56-63.
Khodavandi A, Alizadeh F, Harmal N, Sidik SM, Othman F, Sekawi Z, Farboodniay Jahromi MA, Ng KP, Chong PP. 2011b. Comparison between efficacy of allicin and fluconazole against Candidaalbicansin vitro and in a systemic candidiasis mouse model. FEMS Microbiol Lett, 315: 87-93.
Khodavandi A, Alizadeh F, Namvar F, Rosfarizan M, Chong PP. 2014. Anti-Candida potential of Allium ascalonicum Linn: antibiofilm activity and biomolecular mechanism of action. J Pure Appl Microbiol, 8: 349-356.
Khorsand I, Ghanbari Nehzag MA, Zarrinfar H, Fata A, Naseri A, Badiee P, Najafzadeh MJ. 2015. Frequency of variety of Candida species in women with Candida vaginitis referred to clinical centers of Mashhad, Iran. Iran J Obstet Gynecol Infertil, 18: 15-22.
Klepser ME, Ernst EJ, Lewis RE, Ernst ME, Pfaller MA. 1998.Influence of test conditions on antifungal time-kill curve results: proposed for standardized methods. Antimicrob Agents Chemother, 42: 1207-1212.
Kord Z, Fata A, Zarrinfar H. 2017. Molecular Identification of Candida species isolated from patients with vulvovaginitis for the first time in Mashhad. Iran J Obstet Gynecol Infertil, 20: 50-57.
Kothavade RJ, Kura MM, Valand AG, Panthaki MH. 2010. Candida tropicalis: its prevalence, pathogenicity and increasing resistance to fluconazole. J Med Microbiol, 59: 873-880.
LanzottiV, Bonanomi G, Scala F. 2013. What makes Allium species effective against pathogenic microbes? Phytochem Rev, 12: 751-772.
Leelarungrayub N, Chanarat N, Rattanapanone V. 2004. Potential activity of Thai Shallot (A. Ascalonicum L.) extract on the prevention of hemolysis and glutathione depletion in human erythrocyte from oxidative stress. Chiang Mai Univ J Sci, 3: 225-234.
Li WR, Shi QS, Dai HQ, Liang Q, Xie XB, Huang XM, Zhao GZ, Zhang LX. 2016. Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans. Sci Rep, 6: 22805.
Mendoza-Juache A, Aranda-Romo S, Bermeo-Escalona JR, Gómez-Hernández A, Pozos-Guillén A, Sánchez-Vargas LO. 2016. The essential oil of Allium sativum as an alternative agent against Candida isolated from dental prostheses. Rev Iberoam Micol, 34: 158-164.
Mikaili P, Maadirad S, Moloudizargari M, Aghajanshakeri S, Sarahroodi S. 2013. Therapeutic uses and pharmacological properties of garlic, shallot, and their biologically active compounds. Iran J Basic Med Sci, 16: 1031–1048.
Mnayer D, Fabiano-Tixier AS, Petitcolas E, Hamieh T, Nehme N, Ferrant C, Fernandez X, Chemat F. 2014. Chemical composition, antibacterial and antioxidant activities of six essentials oils from the Alliaceae family. Molecules, 19: 20034-20053.
Odds FC, Brown AJP, Grow NAR. 2003. Antifungal agents: mechanisms of action. TRENDS Microbiol, 11: 272–9.
Omar SH, Al-Wabel NA. 2010. Organosulfur compounds and possible mechanism of garlic in cancer. Saudi Pharm, 18: 51–58.
Palmeira-de-Oliveira A, Silva BM, Palmeira-de-Oliveira R, Martinez-de-Oliveira J, Salgueiro L. 2013. Are plant extracts a potential therapeutic approach for genital infections? Curr Med Chem, 20: 2914-2928.
Sadri A, Khodavandi A, Alizadeh F. 2016. Quorum-sensing quenching compounds Allium sativum, Allium hirtifolium and Allium cepa: the probable quorum- sensing quenching compounds against Candida albicans. Biosci Biotechnol Res Asia, 13: 1457-1468.
Sardi JC, Scorzoni L, Bernardi T, Fusco-Almeida AM, Mendes Giannini MJ. 2013. Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol, 62: 10-24.
Shuford JA, Steckelberg JM, Patel R. 2005. Effects of fresh garlic extract on Candida albicans biofilms. Antimicrob Agents Chemother, 49: 473.
Szychowski KA, Binduga UB, Rybczyńska-Tkaczyk K, Leja ML, Gmiński J. 2016. Cytotoxic effects of two extracts from garlic (Allium sativum L.) cultivars on the human squamous carcinoma cell line SCC-15. Saudi J Biol Sci. doi.org/10.1016/j.sjbs.2016.10.005.
Zarif L, Graybill JR, Perlin D, Najvar L, Bocanegra R, Mannino RJ. 2000. Antifungal activity of amphotericin B cochleates against Candida albicans infection in a mouse model. Antimicrob Agents