Protective effect of Mumiju against acetic acid-induced ulcerative colitis in rats

Document Type : Original Research Article

Authors

1 1. Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

2 natural products and medicinal plants research center, north khorasan university of medical sciences, bojnurd, Iran

3 Endocrinology and Metabolism Research Center, Institute of Basic and clinical physiology, Kerman University of Medical Sciences, Kerman, Iran.

4 4. Payame Noor University, Isfahan, Iran

5 Department of Pathology, University of Medical Sciences, Kerman, Iran

6 School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Abstract

Objective: In this study, we elucidated the ameliorative effect of aqueous extract of leaves of Mumiju against acetic acid-induced experimental colitis in male rats.
Materials and Methods: The animals were randomly divided into four groups (n=7) including I: control group, II: vehicle group (injected with 2 ml acetic acid (4%) intra rectally), III and IV: treatment groups which received Mumiju (250 mg/kg) orally or intraperitoneally for 4 consecutive days after ulcer induction. Ulcer index, severity of inflammation, colonic levels of superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA), and histological changes were recorded after the treatment regimen of 4 days.
Results: The ulcer index, severity of inflammation and colonic MDA levels were increased following intrarectal instillation of acetic acid. Also, acetic acid significantly decreased the SOD and GSH levels. Treatment with Mumiju for 4 days exhibited significantly lowered oxidative stress, while elevated of SOD and GSH levels. Regenerative-healing patterns also was seen by histopathological findings after treatment with Mumiju.
Conclusion: The present investigation demonstrates that Mumiju could be regarded as a herb with potent therapeutic value in the amelioration of experimental colitis in laboratory animals by modulation of oxidant- antioxidant system.

Keywords

Main Subjects


Agarwal SP, Khanna R, Karmarkar R, Anwer MK, Khar RK. 2007. Shilajit: A review. Phytother Res, 21:401–405.
Al-Himaidi AR, Mohammed U. 2003. Safe use of salajeet during the pregnancy of Female mice. Online J Biol Sci, 3:681–684.
Aiello A, Fattorusso E, Menna M, Vitalone R, Schröder HC, Müller WE. 2011. Mumiju traditional medicine: Fossil deposits from antarctica (chemical composition and beneficial bioactivity) Evid Based Complement Alternat Med, 738131. 
Bhattacharya SK, Sen AP, Ghosal S. 1995. Effects of shilajit on biogenic free radicals. Phytother Res, 9:56–59.
Bhattacharya SK, Ghosal S. 1992. Effect of Shilajit on rat brain monoamines. Phytother Res, 6:163–164.
Carty E, De-Brabander M, Feakins RM, Rampton DS. 2000. Measurement of in vivo rectal mucosal cytokine and eicosanoid production in ulcerative colitis using filter paper. Gut, 46; 487–492.
Chavan S, Sava L, Saxena V, Pillai S, Sontakke A, Ingole D. 2005. Reduced glutathione: importance of specimen collection. Indian J Clin Biochem, 20; 150-152.
Cooper HS, Murthy SN, Shah RS, Sedergran DJ. 1993. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest, 69: 238-249.
 Dieleman LA, Palmen MJ, Akol H, Bloemena E, Peٌa AS, Meuwissen SG, Van Rees EP. 1998. Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines. Clin Exp Immunol, 114:385-391.
Fiocchi C. 1998. Inflammatory bowel disease: etiology and pathogenesis. Gastroenterology 115, 182–205.
Frolova LN, Kiseleva TL. 1996. Chemical composition of mumijo and methods for determining its authenticity and quality (a review). Pharm Chem J, 30:543–547.
Garedewa A, Feist M, Schmolz E, Lamprecht I. 2004.Thermal analysis of mumiyo, the legendary folk remedy from the Himalaya region. Thermochim Acta, 417:301–309.
Ghaaazi F ,  Shahrokhi N ,  Khaksari M , Asadikaram G ,  Atashbar J.  2018. Effect of Shilajit on the levels of pro-inflammatory and anti- inflammation cytokines in hepatic injury in male rats. Journal of Mazandaran University of Medical Sciences, 27, 1-13.
Ghannadi A, Zolfaghari B, Shamashian S. 2011. Necessity, importance and applications of traditional medicine in different ethnic. J Tradit Med Islam, 13:161–176.
 Ghosal S. 1990.Chemistry of shilajit, an immunomodulatory ayurvedic rasayan. Pure Appl Chem (IUPAC), 62:1285–1288.
Ghosal SK, Kumar Y, Srivastava R, Goel RK, Dey R, Bhattacharya SK. 1988. Anti-ulcerogenic activity of fulvic acids and 4’-methoxy-6-carbomethoxybiphenyl isolated from shilajit. Phytother Res, 2:187–191.
Ghosal S, Lal J, Singh SK, Dasgupta G, Bhaduri J, Mukhopadhyay M, et al. 1989. Mast cell protecting effects of shilajit and its constituents. Phytother Res, 3:249–252.
Ghosal S, Lal J, Singh SK, Goel RK, Jaiswal AK, Bhattacharya SK. 1991.The need for formulation of shilajit by its isolated active constituents. Phytother Res, 5:211–216
Ghosal SL, Jaiswal AK, Bhattacharya SK. 1993. Effects of Shilajit and its active constituents on learning and memory in rats. Phytother Res, 7:29–34.
Goel RK, Banerjee RS, Acharya SB. 1990. Antiulcerogenic and antiinflammatory studies with shilajit. J Ethnopharmacol, 29:95–103.
Kel’ginbaev NS, Sorokina VA, Stefanidu AG, Ismailova VN. 1973. Treatment of long tubular bone fractures with Mumie Assil preparations in experiments and clinical conditions. Eksp Khir Anesteziol, 18:31–35.
Keshavarzi Z, Khaksari M, Razmi Z, Soltani Hekmat A, Naderi V, Rostami S. 2012. The effects of cyclooxygenase inhibitors on the brain inflammatory response following traumatic brain injury in rats. Iran J Basic Med Sci, 15:1102-1105.
Keshavarzi Z, Alikhani V, Vatanchian M, Tabatabaei Yazdi A, Bibak B, Mohebbati R. 2014. Effects of Aloe Vera Gel on Gastric Acid Secretion and Colon Histopathology in Ulcerative Colitis Model induced by Acetic Acid in Rats. Zanjan University of Medical Sciences, 22: 1-11.
Loftus Jr, EV.2004. Clinical epidemiology of inflammatory bowel disease: incidence, prevalence, and environmental influences. Gastroenterology, 126, 1504–1517.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem. 193:265-275.
Mirza MA, Alam MN, Faiyazuddin M, Mahmood D, Bairwa R, Mustafa G. 2010. Shilajit: An ancient panacea. Int J Curr Pharmaceut Rev Res, 1:2–11.
Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuk MR,Wallace JL. 1989. Hapten-induced model of chronic inflammationand ulceration in the rat colon. Gastroenterology, 96: 795-803.
Nosalova V, Cerna S, Bauer V. 2000. Effect of N-acetylcysteine on colitis induced by acetic acid in rats. Gen Pharmacol, 35, 77–81.
Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351–358.
Phaechamud TJC, Wetwitayaklung P, Limmatvapirat C, Srichan T. 2008. Some biological activities and safety of mineral pitch (mumiju) Silpakorn Univ Sci Technol J, 2:7–17.
Rajesh K, Witt M, Anwer MK, Agarwal SP, Koch BP. 2008. Spectroscopic characterization of fulvic acids extracted from the rock exudate shilajit. Org Geochem, 39:1719–1724.
Repetto MG, Llesuy SF. 2002. Antioxidant properties of natural compounds used in popular medicine for gastric ulcers. Braz J Med Biol Res, 35:523–534.
Sartor RB. 1997. Pathogenesis and immune mechanisms of chronic inflammatory bowel disease. The American Journal of Gastroenterology, 92, 5S–11S.
Shakurov AS. 1965. Effect of “mumie” on bone regeneration and blood alkaline phosphatase in experimental fractures of the tubular bones. Ortop Travmatol Protez, 26:24–27.
Shahrokhi N, Keshavarzi Z, Khaksari M. 2015. Ulcer healing activity of Mumijo aqueous extract against acetic acid induced gastric ulcer in rats. J Pharm Bioallied Sci. 7:56-59.
Spassov V. 1994. Memory effects of the natural product Mumyo on the water maze in rats. Eur Neuropsychopharmacol, 4:396.