Identification of phenolic antioxidants in Ipomoea mauritiana jacq. using spectrophotometric and mass spectroscopic studies

Document Type: Short communication

Authors

Centre for Medicinal Plants Research, Arya Vaidya Sala Kottakkal-676503, Kerala, India

Abstract

Objective: Ipomoea mauritiana is used in both Ayurveda and folk medicine systems. The tuberous roots are known to be diuretic, depurative, carminative, and anthelmintic. The objective of the current study was to identify phenolic antioxidants from I. mauritiana using spectrophotometric and LC-MS analysis.
Materials and Methods: An activity-guided fractionation and purification process was used to identify the antioxidative components from I. mauritiana tuber. Dried mature tubers of I. mauritiana were extracted with 80% methanol and then partitioned by chloroform, ethyl acetate, acetone, and methanol. The acetone fraction showed the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity among four fractions and was subjected to separation and purification using preparative reverse-phase high-performance liquid chromatography (HPLC).
Results: Two compounds were separated from the acetone fraction using preparative LC fraction collector. The purified compounds were screened for their antioxidative potential using DPPH assay. The compounds were subjected to LC-MS analysis in ESI negative mode. One of the compounds was identified as Caffeoyl glucose based on the mass fragmentation.
Conclusion: The acetone fraction showed highest radical scavenging activity and the phytoconstituents of the same were identified by LC-MS/MS analysis.

Keywords

Main Subjects


Anttonen MJ, Karjalainen RO. 2006. High-performance liquid chromatography analysis of black currant (Ribes nigrum L.) fruit phenolics grown either conventionally or organically. J Agric Food Chem,  54: 7530-7538.

Apea-Bah FB, Hanafi M, Dewi RT, Fajriah S, Darmawan A,  Artanti N, Lotulung P, Ngadymang P, and Minarti B. 2009. Assessment of the DPPH and α-glucosidase inhibitory potential of gambier and qualitative identification of major bioactive compound. J Med Plants Res, 3: 736-757.

API 2006. The Ayurvedic Pharmacopoeia of India, Part-I, Volume-V, First Edn., (Government of India, Ministry of Health and Family Welfare, Department of Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homoeopathy (AYUSH), New Delhi, 88.

Astley SB, 2003. Dietary antioxidants past, present and future. Trends Food Sci. Technol, 14: 93-98.

Atoui A, Mansouri A, Boskou G, Kefalas P. 2005. Tea and herbal infusions: their antioxidant activity and phenolic profile. Food Chem, 89: 27-36.

Chopra RN, Nayar SL, Chopra IC. 1992. Glossary of Indian Medicinal Plants, Council of Scientific and Industrial Research (CSIR), New Delhi, 142.

Gandía-Herrero F, Escribano J. 2009. García-Carmona F The role of phenolic hydroxy groups in the free radical scavenging activity of betalains. J Nat Prod, 72: 1142-1146

Gao JJ, Igalashi K, Nukina M. 1999. Radical scavenging activity of phenylpropanoid glycosides in Caryopteris incana. Biosci Biotechnol Biochem, 63: 983-988.

Halliwell B, Gutteridge JMC. 1989. Free Radicals in Biology and Medicine, 2nd ed.; Clarendon Press: Oxford, UK.

Harborne JB and Boxter H. 1995. Phytochemical Dictionary, Taylor & Francis, 323-325.

Heinonen M, Meyer AS, Frankel EN. 1998. Antioxidant activity of berry phenolics on human low-density lipoprotein and liposome oxidation. J Agric Food Chem, 46: 4107-4112

Karthik, S, Chandrakala C, Venkatasubramanian P. 2009. Phytochemical and Microscopic analysis of tubers of Ipomea mauritiana Jacq. (Convolvulaceae), Pharmacognosy Magazine, 4: 272-278.

Khan M. Sajjad, Nema Nitin, MD, Kharya, Khanam Salma. 2009. Chromatographic estimation of maturity based phytochemical profiling of Ipomoea mauritiana International Journal of Phytomedicine 1: 22-30.

Lapornik A, Prosek M, Wondra GA. 2005. Comparision of extracts prepared from plant byproducts using different solvents and extraction time. J. Food Eng, 71: 214-222.

Liu R, Sun J, Bi K, Guo DA. 2005. Identification and determination of major flavonoids in rat serum by HPLC-UV and HPLC-MS methods following oral administration of Dalbergia odorifera extract. J Chromatogr. B, 829: 35-44.

Moushumi SJ, Ahmed R, Ahmed H, Ali M, Haq WM, Jahan R,  Rahmatullah M. 2010. Hypoglycemic, Hypocholesterolemic and hypotriglyceridemic activity of tuber roots of Ipomoea mauritiana Jacq. (Convolvulaceae) when administered to rats. Adv Nat Appl Sci, 4: 174-176.

Pandey G. 2004. Dravyaguna Vijnana, Part III, Chowkhamba Krishnadas Academy, Varanasi (Reprint) 852.

Pulok K, Mukerjee. 2002. Quality control of herbal drugs, Business Horizone pharmaceutical publishers, 15-16.

Shahidi F. 2000. Antioxidants in food and food antioxidants. Food / Nahrung 44:158-63

Singleton VL and Rossi Jr JA .1965. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents, Am J Enol Viticult, 16: 144-158.

Sivanandham V. 2011. Free radicals in health and diseases ─ a mini review, Pharmacol Onl, 1: 1062-1077.

Sivarajan VV and Balachandran I. 1994. Ayurvedic Drugs and Their Plant Sources, Oxford and IBH Publishing, New Delhi, India, pp. 510-512

Tepe B, Daferera D, Sokmen A, Sokmen M and Polissiou M. 2005. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food Chem, 90: 333-340.

Ved DK and Goraya GS. 2008. Demand and Supply of Medicinal Plants in India, Bishen SinghMahendra Pal Singh, Dehradun, India,

Venkatasubramanian P, SK  Kumar, and SN Venugopal. 2009. Use of ‘Kshiravidari’ as a substitute for ‘Vidri’ as per Ayurvedic descriptions. Indian J Trad Kdge, 8: 310-318,

Warrier PK, Nambiar VPK.  Ramankutty C. 1995 Indian Medicinal Plants: A Compendium of 500 Species, Orient Longman, Chennai, India Vol. 3. pp. 222-228.

Zheng W and Wang SY. 2001. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem, 49: 5165-5170.