Comparison between effects of different doses of Melissa officinalis and atorvastatin on the activity of liver enzymes in hypercholesterolemia rats

Document Type : Short communication

Authors

1 Department of Biology, Unit of Science Research, Islamic Azad University of Damghan, Damghan, I.R. Iran

2 Department of Physiology, Arak University of Medical Sciences, Arak, I. R. Iran

3 Deputy of Research, Arak University of Medical Sciences, Arak, I. R. Iran

4 Department of Plant Biology, Payame-Noor University, Tehran, I. R. Iran

Abstract

Objectives: Liver is one of the most sensitive tissues to oxidant damage. Hence, the present study was conducted to compare the effects of Melissa officinalis (MO) extract and Atorvastatin on the activity of liver enzymes in rats.
Materials and Methods: In this experimental study, 60 male Wistar rats were selected and allocated to six groups (n=10). The control group received a normal diet, sham group received a fatty diet while other groups received a fatty diet and the alcoholic extract ofMO, at minimum (25 mg/kg), moderate (50 mg/kg), and maximum (75 mg/kg) doses (i.p.). The last group received Atorvastatin (10 mg/kg) through gavage with a fatty diet over a 21-day period. At the end of this 21-day period, blood samples were drawn and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), activity of liver enzymes as well as cholesterol in the samples were measured.
Results: The obtained results showed that the activity of liver enzymes in the treatment groups receiving MO extract and the group receiving Atorvastatin decreased significantly. MOextract reduced the level of liver enzymes.
Conclusion: Therefore, further studies for obtaining a better understanding of the mechanism of effect of MO for treating liver diseases are recommended.

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Main Subjects

References

Akhondzadeh S, Noroozian M, Mohammadi M, Ohadinia S, Jamshidi AH, et al. 2003. Melissa officinalis extract in the treatment of patients with mild to moderate Alzheimer's disease: a double blind, randomised, placebo controlled trial. J Neurol Neurosurg Psychiatry, 74: 863-866.
Al-Qarawi AA,  Abdel-Rahman HA, Ali BH, El Mougy SA. 2002. Liquorice (Glycyrrhiza glabra) and the adrenal-kidney-pituitary axis in rats. Food Chem Toxicol, 40: 1525-1527.
Ahmad A, Pillai KK, Najmi AK, Ahmad SJ, Pal SN et al. 2002. Evaluation of hepatoprotective potential of jigrine post-treatment against thioacetamide induced hepatic damage. J Ethnopharmacol, 79: 35-41.
Ashtiyani SC, Zarei A, Taheri S, Rasekh F. 2011. The effects of Portulaca Oleracea extract on induced hypercholesteroleomia in rats. ZJRMS, 13: 20-24.
Barcelo S, Gardiner JM, Gescher A, Chipman JK . 1996. CYP2E1-mediated mechanism of anti-genotoxicity of the broccoli constituent sulforaphane. Carcinogenesis, 17: 277-282.
Belfield A, Goldberg DM . 1971. Revised assay for serum phenyl phosphatase activity using 4-amino-antipyrine. Enzyme, 12: 561-573.
Bhandari U, Sharma JN, Zafar R. 1998. The protective action of ethanolic ginger (Zingiber officinale) extract in cholesterol fed rabbits. J Ethnopharmacol, 61:167-171.
Blumenthal M, Goldberg A, Brinckmann J. 2000. Herbal Medicine: Expanded Commission E Monographs. pp. 230-232,1th ED. Integrative Medicine Communications press.
Bolkent S, Yanardag R, Karabulut-Bulan O, Yesilyaprak B. 2005. Protective role of Melissa officinalis L. extract on liver of hyperlipidemic rats: a morphological and biochemical study. J Ethnopharmacol, 99: 391-398.
Bush BM .1992. Interpretation of Laboratory Results for Small Animal Clinicians. pp. 408-410, 1th ED. Wiley-Blackwell Publication.
De Carvalho NC, Correa-Angeloni MJ, Leffa DD, Moreira J, Nicolau V, de Aguiar Amaral P, et al. 2011. Evaluation of the genotoxic and antigenotoxic potential of Melissa officinalis in mice. Genet Mol Biol, 34: 290-297.
Emamghorishi M, Talebianpour MS. 2009. Antidepressant effect of Melissa officinalis in the forced swimming test. DARU J Pharm Sci, 17: 42-47.
Gainsford T, Willson TA, Metcalf D, Handman E, McFarlane C, Ng A, et al .1996. Leptin can induce proliferation, differentiation, and functional activation of hemopoietic cells. Proc Natl Acad Sci USA, 93:14564-14568.
Gatreh-Samani K, Gatreh-Samani K, Khalili B, Rafieian M, Moradi MT. 2011. Purslane (Portulaca oleracea) effects on serum paraoxanase-1 activity. Shahrekord Univ Med Sci, 13: 9-15.
Germanò MP, Sanogo R, Costa C, Fulco R, D'Angelo V, et al.1999. Hepatoprotective properties in the rat of Mitracarpus scaber (Rubiaceae). J Pharm Pharmacol, 51: 729-734.
Hersoug LG, Linneberg A. 2007. The link between the epidemics of obesity and allergic diseases: does obesity induce decreased immune tolerance? Allergy, 62: 1205-1213.
Imami A, Shams-Ardekani MR, Mehregan I. 2003. The illustrated encyclopedia of herbal medicines. Translated to Persian and edited from a text by Chiej, Roberto entitled: The Macdonald Encyclopedia of Medicinal plants. Research center for traditional medicine and Pharmacognosy, 192, Tehran, Shahid Beheshti University of Medical Sciences press.
Jelodar GA, Nazifi habibabadi S.1997. Effect of celery, sour apple and carrot on some of serum biochemical parameters of diabetic rats. J Kerman Univ Med Sci, 4: 114-119.
Kelly GE، Husband AJ. 2003. Flavonoids compounds in the Preventionof prostate cancer. Methods Mol Med, 81: 377-394
Kennedy DO, Scholey AB, Tildesley NT, Perry EK, Wesnes KA .2002. Modulation of mood and cognitive performance following acute administration of Melissa officinalis (lemon balm). Pharmacol Biochem Behav, 72: 953-964.
Lee J, Chae K, Ha J, Park BY, Lee HS et al. 2008. Regulation of obesity and lipid disorders by herbal extracts from Morus alba, Melissa officinalis, and Artemisia capillaris in high-fat diet-induced obese mice.                              J Ethnopharmacol, 116: 576.
Mitra SK, Venkataranganna MV, Sundaram R, Gopumadhavan S.1998. Protective effect of HD-03, a herbal formulation, against various hepatotoxic agents in rats.                                           J Ethnopharmacol, 63: 181-186.
Nazari A, Delfan B, Shahsavari G. 2005. The effect of Satureja khuzestanica on triglyceride, glucose, Creatinine and Alkaline phosphatase activity in rat. J Shahrekord Univ Med Sci, 7: 1-8
Pereira RP, Fachinetto R, de Souza Prestes A, Puntel RL, Santos da Silva GN, et al. 2009. Antioxidant effects of different extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus. Neurochem Res, 34: 973-983.
Perez-Carreon JI, Cruz-Jiménez G, Licea-Vega JA, Arce Popoca E, Fattel Fazenda S, et al .2002. Genotoxic and anti-genotoxic properties of Calendula officinalis extracts in rat liver cell cultures treated with diethylnitrosamine. Toxicol In Vitro, 16: 253-258.
Pourjafar M, Mohebbi A, Nazifi S, Tarakeme S. 2010. Invitro Study of Effect of High-Fat Diet on Serum Leptin and Thyroid Hormone Levels in Mice. JSSU, 18: 428-435.
Pyo YH, Lee TC, Logendra L, Rosen R.T 2004. Antioxidant activity and phenolic compounds of Swiss chard (Beta vulgaris subspecies cycla) extracts. Food Chem, 85: 19-26.
Reitman S, Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol, 28: 56-63.
Saeb M , Nazifi S , Sabet  M, Nazem H, Gheisari HR, et al. 2010. Effect of dietary wild pistachio oil on serum thyroid hormones, lipids and leptin concentration in experimental hyperthyroidism in male Rat. J Gorgan Univ  Med Sci, 11: 8-15
Saija A, Scalese M, Lanza M, Marzullo D, Bonina F, Castelli F 1995. Flavonoids as antioxidant agents: importance of their interaction with biomembranes. Free Radic Biol Med, 19: 481-486.
Sanz N, Díez-Fernández C, Fernández-Simón L, Alvarez A, Cascales M. 1998. Necrogenic and regenerative responses of liver of newly weaned rats against a sublethal dose of thioacetamide. Biochim Biophys Acta, 1384: 66-78.
Schulz V, Hansel R, Tyler V 2004. Rational Phytotherapy: a physician’s guide to herbal medicine, 5th Ed. New York, Springer Press. 63-68.
Shafie-Zadeh  F. 2002.Lorestan medicinal plants. Lorestan University of Medical Sciences. Hayyan press. 32.
Shariati M, Zarei A. 2006. The study of Physalis Alkekengi extract on liver function. Azad University of Kazeron, (MSc Thesis).
Sun F, Hayami S, Ogiri Y, Haruna S, Tanaka K, et al. 2000. Evaluation of oxidative stress based on lipid hydroperoxide, vitamin C and vitamin E during apoptosis and necrosis caused by thioacetamide in rat liver. Biochim Biophys Acta, 1500: 181-185.
Toit R, Volsteedt Y and Apostolides Z. 2007. Comparison of the antioxidant content of fruits, vegetable and teas measured as vitamin C equivalents. Toxicology, 166: 63-69.
Tohidi M, Harati H, Hadaegh, F, Mehrabi Y, Azizi F. 2007. Association of activity of liver enzymes with incident type 2 diabetes: Tehran lipid glucose study. Iran J  Diabet Lipid Dis, 7: 167-176.
Wake G, Court J, Pikering A, et al. 2000. CNS acetylcholine receptor activity in European medicinal plants traditionally used to improve failing memory. J Ethnopharmacol, 69: 105-114.
Yang CS, Landau JM, Huang MT, Newmark HL. 2001. Inhibition of carcinogenesis by dietary polyphenolic compounds. Annu Rev Nutr, 21: 381-406.
Yoshikava M, Xu F, Morikava T, Ninomya K, Matsuda H. 2003. Anaststins A and B, new skeletal flavonoids with hepatoprotective activities from the desert plant Anastatica hierochuntica. Bioorg  Med chem Lett, 13: 1045-1049.
Zargari A. 1991. Medicinal plants. Tehran University Publications. Tehran. 77-83
 
Avicenna Journal of Phytomedicine
Volume 4, Issue 1 - Serial Number 1
January and February 2014
Pages 15-23

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