Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79309320190501Capsaicin inhibitory effects on Vibrio cholerae toxin genes expression1871941173610.22038/ajp.2018.11736ENSoroor ErfanimaneshInfectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranGita EslamiInfectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranArezou TaherpourDepartment of Microbiology, Kurdistan University of Medical Sciences, Sanandaj, IranAli HashemiDepratment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran0000-0002-7258-5541Journal Article20170615<strong><span style="font-size: medium;">Objective: </span></strong><span style="font-size: medium;">Cholera is an acute secretory diarrhea caused by the Gram-negative bacterium, <em>Vibrio cholerae </em></span><span style="font-size: medium;">mostly through production of cholera toxin (CT) and zonula occludens toxin (Zot)</span><em><span style="font-size: medium;">.</span></em><span style="font-size: medium;"> Isolates of </span><em><span style="font-size: medium;">V. cholerae</span></em><span style="font-size: medium;"> have acquired resistance elements during the last decade. One of the most promising ways to treat resistant strains is to use antivirulence agents instead of killing the causative agent with conventional antibiotics. In this study, we examined whether different concentrations of capsaicin - the pungent fraction of red chili- can act as an antivirulence agent and inhibit </span><em><span style="font-size: medium;">V. cholerae</span></em><span style="font-size: medium;"> toxin production.</span><br /> <strong><span style="font-size: medium;">Materials and Methods:</span></strong><span style="font-size: medium;"> Two standard strains namely, <em>V. cholerae </em></span><span style="font-size: medium;">ATCC 14035 and </span><em><span style="font-size: medium;">V. cholerae</span></em><span style="font-size: medium;"> PTCC 1611 were used in this study.</span><span style="font-size: medium;"> Minimum Inhibitory Concentration (MIC) of capsaicin </span><span style="font-size: medium;">was determined by broth microdilution method. Based on MIC results, the bacteria were cultured in the presence of sub-MIC concentrations of capsaicin and a negative control without capsaicin. Real-time PCR (RT-PCR) was carried out to determine the expression level of </span><em><span style="font-size: medium;">V. cholerae </span></em><span style="font-size: medium;">toxin genes at each concentration.</span><br /> <strong><span style="font-size: medium;">Results:</span></strong><span style="font-size: medium;"> MIC test showed that 200 mg/mL of capsaicin in 2% dimethyl sulfoxide (DMSO) could inhibit the growth of the two standard strains of <em>V. cholera</em></span><span style="font-size: medium;">e. The expression of </span><em><span style="font-size: medium;">V. cholerae </span></em><span style="font-size: medium;">toxin genes was significantly reduced following treatment with sub-MIC concentrations of capsaicin as assessed by RT-PCR.</span><br /> <strong><span style="font-size: medium;">Conclusion:</span></strong><span style="font-size: medium;"> Capsaicin showed great inhibitory effect against cholera toxin and reduced Zot production in the tested strains of <em>V. cholerae</em></span><span style="font-size: medium;">. The results showed promising insights into antivirulence effects of capsaicin.</span>Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79309320190501Clinical and experimental effects of Nigella sativa and its constituents on respiratory and allergic disorders1952121219610.22038/ajp.2019.12196ENZahra GholamnezhadNeurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.0000-0001-6280-7246Farzaneh ShakeriNatural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, IranSaiedeh SaadatNeurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranVahideh GhoraniDepartment of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranPharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran0000-0001-6883-7972Mohammad Hossein BoskabadyNeurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.0000-0001-5736-9755Journal Article20181106<strong>Objective: </strong>Black cumin or <em>Nigella sativa</em> (<em>N. sativa</em>) seed has been widely used traditionally as a medicinal natural product because of its therapeutic effects. In this review, the medicinal properties of <em>N. sativa</em> as a healing remedy for the treatment of respiratory and allergic diseases, were evaluated.<br /> <strong>Material and Methods: </strong>Keywords including <em>Nigella sativa</em>, black seed, thymoquinone, respiratory, pulmonary, lung and allergic diseases were searched in medical and nonmedical databases (i.e. PubMed, Science Direct, Scopus, and Google Scholar). Preclinical studies and clinical trials published between 1993 and 2018 were selected.<br /> <strong>Results:</strong> In experimental and clinical studies, antioxidant, immunomodulatory, anti-inflammatory, antihistaminic, antiallergic, antitussive and bronchodilatory properties of <em>N. sativa</em> different extracts, extracts fractions and constituents were demonstrated. Clinical studies also showed bronchodilatory and preventive properties of the plant in asthmatic patients. The extract of <em>N. sativa</em> showed a preventive effect on lung disorders caused by sulfur mustard exposure. The therapeutic effects of the plant and its constituents on various allergic disorders were also demonstrated.<br /> <strong>Conclusion: </strong>Therefore, <em>N. sativa</em> and its constituents may be considered effective remedies for treatment of allergic and obstructive lung diseases as well as other respiratory diseases.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79309320190501Protective effect of Berberis vulgaris on Fenton reaction-induced DNA cleavage2132201178810.22038/ajp.2018.11788ENNooshin Sadat AsadiDepartment of Biology, Faculty of Science, Yazd University, Yazd, IranMohammad Mehdi HeidariDepartment of Biology, Faculty of Science, Yazd University, Yazd, Iran0000-0002-3328-4746Mehri KhatamiDepartment of Biology, Faculty of Science, Yazd University, Yazd, IranJournal Article20171201<span style="font-size: medium;"><strong>Objective:</strong><em> Berberis vulgaris</em></span><span style="font-size: medium;"> contains antioxidants that can inhibit DNA cleavage. The purpose of this study was to evaluate the antioxidant and protective activity of <em>B. vulgaris</em></span><span style="font-size: medium;"> on DNA cleavage.</span><br /> <strong><span style="font-size: medium;">Materials and Methods:</span></strong><span style="font-size: medium;"> In this study, the antioxidant capacity of <em>B. vulgaris </em></span><span style="font-size: medium;">was investigated using DPPH and its protective effect was evaluated on </span><em><span style="font-size: medium;">pBR322</span></em><span style="font-size: medium;"> plasmid and lymphocyte genomic DNA cleavage induced by Fenton reaction, by DNA electrophoresis.</span><br /> <strong><span style="font-size: medium;">Results:</span></strong><span style="font-size: medium;"> Aqueous extract of <em>B. vulgaris</em></span><span style="font-size: medium;"> presented dual behavior with a potent antioxidant activity at 0.25and 0.75mg/ml for pBR322 plasmid and lymphocyte genomic DNA, respectively, but a pro-oxidant activity was observed at higher concentrations.</span><br /> <strong><span style="font-size: medium;">Conclusion:</span></strong><span style="font-size: medium;"> Our results indicated that <em>B. vulgaris</em></span><span style="font-size: medium;"> extract an inhibit Fenton reaction-induced DNA cleavage and oxidative cleavage of double-stranded DNA assay is a powerful technique that can be used to determine the antioxidant and pro-oxidant properties of a compound on cellular components such as DNA.</span>Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79309320190501Anti-inflammatory and anti-neuropathic effects of a novel quinic acid derivative from Acanthus syriacus2212361221810.22038/ajp.2019.12218ENKarim MRaafatDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Beirut Arab University, 115020 Beirut, Lebanon0000-0001-5907-6956Journal Article20180827<span style="font-size: medium;"><strong>Objective: </strong><em>Acanthus syriacus</em></span><span style="font-size: medium;"> (AS) is one of the valuable herbal plants with immunomodulatory potentials. The aim of this study is to assemble a phytochemical investigation of <em>A. syriacus</em></span><span style="font-size: medium;"> exploring its anti-inflammatory and antinociceptive properties, identification of its most active compound(s) and elucidating their structure and determining their mechanisms of action. </span><br /> <strong><span style="font-size: medium;">Materials and Methods:</span></strong><span style="font-size: medium;"> Bio-guided fractionation and isolation-schemes were used utilizing RP-HPLC, CC, </span><sup><span style="font-size: small;">1</span></sup><span style="font-size: medium;">H- and </span><sup><span style="font-size: small;">13</span></sup><span style="font-size: medium;">C-NMR, and biological-models were used to evaluate their effects against inflammation and neuropathic-pain (NP). </span><br /> <span style="font-size: medium;"><strong>Results:</strong> The outcomes showed that the most active fraction (FKCA) of AS was identified. Two of the three components of FKCA were identified by chromatographic-methods, while the third compound was isolated, its structure was elucidated and its was named Kromeic acid (KRA); FKCA contained Ferulic acid (27.5%), kromeic acid (48.1%), and chlorogenic acid (24.4%). AS, FKCA and KRA showed significant (p˂0.05) anti-inflammatory and antinociceptive potentials in the management of allodynia and thermal-hyperalgesia in NP. AS and FCKA showed comparatively equipotent antinociceptive-effects. FKCA showed higher antinociceptive effects than KRA suggesting additive-effects among FKCA components. The anti-inflammatory, insulin secretagogue, oxidative-stress reducing, and protective effects against NO-induced neuronal-toxicity might be amongst the possible mechanisms of tested compounds to alleviate NP. </span><br /> <strong><span style="font-size: medium;">Conclusion:</span></strong><span style="font-size: medium;"> Here, we report the isolation and structure elucidation of a novel quinic-acid derivative, KRA. <em>A. syriacus,</em></span><span style="font-size: medium;"> FKCA, and KRA might be used as a novel complementary approach to ameliorate a variety of painful-syndromes.</span>Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79309320190501Interactive anticancer effect of nanomicellar curcumin and galbanic acid combination therapy with some common chemotherapeutics in colon carcinoma cells2372471226510.22038/ajp.2019.12265ENArash JafariSchool of Medicine, Birjand University of Medical Sciences, Birjand, IranManouchehr TeymouriNatural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.0000-0002-7855-4519Maryam Ebrahimi NikNanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranAzam AbbasiBiotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranMehrdad IranshahiBiotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranMohammad Yahya Hanafi-BojdCellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, IranMahmoud Reza JafariNanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.0000-0003-3908-6828Journal Article20180905<span style="font-size: medium;"><strong>Objective:</strong> In the current investigation, we aimed to study the combined cytotoxicity of curcumin, as a nanomicellar formulation, and galbanic acid (Gal), dissolved in DMSO against the murine C26 and human Caco-2 colon carcinoma cells. Further, curcumin potential for cisplatin and doxorubicin (Dox) co-therapy was studied. </span><br /> <span style="font-size: medium;"><strong>Materials and Methods:</strong> The combined cytotoxic effect of these phytochemicals at varying dose ratios were examined using the MTT colorimetric assay. Moreover, the time-dependent toxicity of curcumin, cisplatin, Dox, and pegylated liposomal Dox (Doxil) was determined. The interactive anti-proliferative behavior of these compounds was examined using the CompuSyn software. </span><br /> <span style="font-size: medium;"><strong>Results:</strong> Nanomicellar curcumin showed considerable cytotoxicity in C26 cells 24 hr post-treatment. Co-treatment of cells with curcumin nanomicelles: Gal had a synergistic effect in C26 (at 10:1 molar ratio), and Caco-2 (at 1:5 molar ratio) cell lines in cell cultures. Nanomicellar curcumin showed strong and mild synergistic inhibitory effects in C26 cells when co-administered with Doxil and cisplatin, respectively. </span><br /> <span style="font-size: medium;"><strong>Conclusion:</strong> Curcumin nanomicelles and Gal had a synergistic effect in C26 and Caco-2 cell lines. It is speculated that nanomicellar curcumin shows synergistic cancer cell killing if administered 24-hr post-injection of Doxil and cisplatin. </span>Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79309320190501Evaluation of the protective and therapeutic effects of Pistacia atlantica gum aqueous extract on cellular and pathological aspects of experimental asthma in Balb/c mice2482591231010.22038/ajp.2019.12310ENZaynab ShakramiDepartment of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.Hadi Esmaeili Gouvarchin GhalehApplied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Bahman Mansouri MotlaghDepartment of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranAli SheikhianRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences.0000-0002-7048-7710Bahman Jalali KondoriFaculty of medicine, Baqiyatallah University of medical sciences, Tehran, Iran0000-0002-6134-9965Journal Article20180605<span style="font-size: medium;"><strong>Objective:</strong> </span><span style="font-size: medium;">The purpose of this study was to investigate the protective and therapeutic effects of aqueous extract of <em>P. atlantica </em></span><span style="font-size: medium;">gum on an experimental asthma in BALB/c mice.</span><br /> <span style="font-size: medium;"><strong>Materials and Methods:</strong> Aqueous extract </span><span style="font-size: medium;">of dried and milled <em>P. atlantica </em></span><span style="font-size: medium;">gum was assemble and</span><span style="font-size: medium;">evaluate by GC-MS. </span><span style="font-size: medium;">In order to investigate the effect of </span><em><span style="font-size: medium;">P. atlantica </span></em><span style="font-size: medium;">gum extract on cellular and pathological aspects of asthma, 60 BALB/c mice were divided into six groups as: negative control, asthmatic group, asthmatic group receiving dexamethasone (1mg/kg; intraperitoneal (IP)) and three asthmatic groups receiving different concentrations of the extract (100, 200 and 400 mg/kg, orally) from the beginning of the study and continued for 84 days. The examined parameters included cell population, IgE antibody production, levels of IL-4, IL-5,</span><span style="font-size: medium;"> TGF-β, INF-γ, IL-10, and IL-17 cytokines, and lung tissue damage. </span><br /> <strong><span style="font-size: medium;">Results:</span></strong><span style="font-size: medium;"> Regardless of the dose, aqueous extract of <em>P. atlantica </em></span><span style="font-size: medium;">gum, caused significant decrease in the number of BALF eosinophilic cells and levels of anti-ovalbumin IgE, IL-4, IL-5 and IL-17 cytokine levels, as well as pathologic damage of the lung tissue. In addition, the amount of anti-inflammatory IL-10, TGF-β, and INF-γ Th1 cytokines significantly increased in the extract-treated groups compared to the asthmatic and dexamethasone-treated groups. Moreover, IFN-γ/IL-4 ratio significantly increased in a dose-dependent manner compared to the un-treated asthma group. </span><br /> <strong><span style="font-size: medium;">Conclusion:</span></strong><span style="font-size: medium;"> The aqueous extract of <em>P. atlantica </em></span><span style="font-size: medium;">gum can be considered as a potent anti-inflammatory and immunomodulatory compound and </span>may be used as a natural compound for treatment of immune system disorders.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79309320190501Effect of ointment-based egg white on healing of second- degree wound in burn patients: a triple-blind randomized clinical trial study2602701259710.22038/ajp.2019.12597ENSimin JahaniNursing Care Research Center in Chronic Diseases, Nursing and Midwifery School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Hadis AshrafizadehNursing and Midwifery School, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.0000-0002-9369-5854Kamran BabaiDepartment of Plastic Surgery, Taleghani Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Amir SiahpooshMedical Plants Research Center and Department of Pharmacology, School of Pharmacy, Jundishapur University of Medical Sciences, Ahvaz, Iran.Bahman CheraghianDepartment of Biostatistics and Epidemiology, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Journal Article20190122<strong>Objective:</strong> Burn wound healing is one of the problems of medical sciences and it is of great importance to find a drug or substance that can heal burn wounds with minimum complications. The present study aimed to evaluate the effect of ointment-based egg white on healing second-degree burn wounds.<br /> <strong>Materials and Methods:</strong> In the present triple-blind clinical trial, a total of 90 patients from Taleghani hospital, Ahvaz, Iran were selected and randomly divided into two groups based on the inclusion criteria. The intervention group was dressed with egg white formulation + silver sulfadiazine cream and the control group was treated with placebo + silver sulfadiazine cream. The burn wound healing process was evaluated on days 1, 7 and 15 by the Bates-Jensen wound assessment tool.<br /> <strong>Results:</strong> The mean scores of wound healing were decreased (13.75±1.83) in the intervention group when compared to the control (21.51±5.7) on day 15 (p<0.001). The mean duration of wound healing, wound depth, edges, undermining, necrotic tissue, amount of necrosis, exudate type and amount, surrounding skin color, wound induration, peripheral edema, granulation, and epithelialization were significantly decrease in intervention group in comparison with control (p<0.001).<br /> <strong>Conclusion:</strong> The findings of this research showed that egg whites formulation is an appropriate treatment for burn wound healing, reduced above-noted burn wounds’ variables. It seems that this treatment, along with the common medicine, improves chronic wound recovery rate and patients’ health status.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79309320190501The neuroprotective mechanism of cinnamaldehyde against amyloid-β in neuronal SHSY5Y cell line: The role of N-methyl-D-aspartate, ryanodine, and adenosine receptors and glycogen synthase kinase-3β2712801238510.22038/ajp.2019.12385ENMasoumeh EmamghoreishiDepartment of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.Majid RezaFarrokhiShiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, IranAtena AmiriShiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, IranMojtaba KeshavarzShiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.0000-0003-2863-1309Journal Article20180702<span style="font-size: medium;"><strong>Objective:</strong> Cinnamaldehyde may be responsible for some health benefits of cinnamon such as its neuroprotective effects. We aimed to investigate the cinnamaldehyde neuroprotective effects against amyloid beta (Aβ) in neuronal SHSY5Y cells and evaluate the contribution of N-methyl-D-aspartate (NMDA), ryanodine, and adenosine receptors and glycogen synthase kinase (GSK)-3β, to its neuroprotective effects. </span><br /> <span style="font-size: medium;"><strong>Materials and Methods:</strong> After seeding the cells in 96-well plates, adenosine (20, 40, 80, and 120 µM), NMDA (20, 40, 80, and 120 µM), and dantrolene (as a ryanodine receptor antagonist; 2, 4, 6, 8, and 16 µM) were added to the medium containing Aβ25-35 and/or cinnamaldehyde. The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide method was used to assess neurotoxicity and western blot to measure the GSK-3β protein level. </span><br /> <span style="font-size: medium;"><strong>Results:</strong> Cinnamaldehyde (15, 20, 23, and 25 μM) significantly reversed Aβ-induced toxicity in SHSY5Y neuronal cells. Adenosine (20, 40, 80 and 120 μM) inhibited the neuroprotective effects of cinnamaldehyde (15 μM). NMDA (20, 40, 80, and 120 μM) reduced cinnamaldehyde (15 and 23 μM) neuroprotective effects against Aβ neurotoxicity. Dantrolene (2, 4, 8, and 16 μM) significantly reduced cinnamaldehyde (15 μM) neuroprotective effects. Cinnamaldehyde (15 and 23 μM) suppressed the Aβ-induced increment of GSK-3β protein level. </span><br /> <strong>Conclusion:</strong> NMDA and adenosine receptors suppression together with ryanodine receptors stimulation may be relevant to cinnamaldehyde neuroprotective effects against Aβ neurotoxicity. Moreover, the inhibition of GSK-3β may contribute to the cinnamaldehyde neuroprotection.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79309320190501Effects of Boswellia serrata resin extract on motor dysfunction and brain oxidative stress in an experimental model of Parkinson’s disease2812901240310.22038/ajp.2019.12403ENParvaneh DoaeeDepartment of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.Ziba RajaeiDepartment of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.0000-0002-5821-5464Mehrdad RoghaniDepartment of Physiology, School of Medicine, Shahed University of Medical Sciences, Tehran, Iran.Hojjatallah AlaeiDepartment of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I. R. IranMohammad KamalinejadDepartment of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran0009-0006-6373-1320Journal Article20180820<strong><span><span style="font-size: medium;">Objective:</span></span></strong><span><em><span style="font-size: medium;">Boswellia serrata </span></em></span><span lang="EN-CA"><span style="font-size: medium;">oleo-gum resin </span></span><span><span style="font-size: medium;">(frankincense) exerted antioxidant and anti-inflammatory effects against several diseases, such as; asthma, rheumatoid arthritis and irritable bowel syndrome. In the current study, the influences of </span><em><span style="font-size: medium;">B. serrata </span></em><span style="font-size: medium;">resin extract</span><span style="font-size: medium;">on motor dysfunction and oxidative stress markers were investigated in the intrastriatal </span></span><span lang="EN-CA"><span style="font-size: medium;">6-hydroxydopamine</span></span><span><span style="font-size: medium;"> (6-OHDA) model of Parkinson’s disease (PD). </span></span><br /> <strong><span><span style="font-size: medium;">Materials and Methods:</span></span></strong><span><span style="font-size: medium;">The animals were randomly assigned to sham, lesion (6-OHDA), and three lesion groups treated with e</span></span><span><span style="font-size: medium;">thyl alcoholic extract of </span><em><span><span style="font-size: medium;">B. serrata </span></span></em><span><span style="font-size: medium;">at doses of 125, 250 and 500 mg/kg for 3 weeks. </span></span></span><span><span style="font-size: medium;">The neurotoxin 6-OHDA (12.5 µg) was microinjected into the left striatum to induce PD in male rats. Motor behavior was assessed by rotational and elevated narrow beam tests. Oxidative stress markers were measured in striatal and midbrain homogenates. </span></span><br /> <strong><span><span style="font-size: medium;">Results:</span></span></strong><span><span style="font-size: medium;"> There was a significant increase in contralateral rotations in 6-OHDA group versus sham group (p<0.001), and treatment with </span><em><span style="font-size: medium;">B. serrata </span></em><span style="font-size: medium;">resin extract at doses of 125 and 250 mg/kg significantly decreased the rotations in comparison to 6-OHDA group (p<em><span style="font-size: medium;">B. serrata</span></em><span style="font-size: medium;"> extract at doses of 125, 250 and 500 mg/kg caused a significant reduction in the latency and total time (p<0.001, p<0.001, and p<0.01, respectively). Biochemical analysis showed no significant difference in oxidative stress markers levels among the groups.</span></span><br /> <strong><span><span style="font-size: medium;">Conclusion:</span></span></strong><span><span style="font-size: medium;"> Our findings suggest that </span><em><span style="font-size: medium;">B. serrata</span></em><span style="font-size: medium;"> resin extract acts as an anti-inflammatory and antioxidant agent that protects nigrostriatal dopaminergic neurons and improve motor impairments in PD.</span></span></span>