Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79307220170301Gustav III’s risk assessment on coffee consumption; A medical history report99100787010.22038/ajp.2016.7870ENReza AfshariEnvironmental Health Services, BC Centre for Disease Control, Vancouver, CanadaJournal Article20160705<br /> Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79307220170301In vitro and in vivo assessment of inhibitory effect of stevioside on pro-inflammatory cytokines101106659910.22038/ajp.2016.6599ENJatuporn NoosudDepartment of Companion Animal Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, ThailandNarissara LailerdDepartment of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandAutchara KayanDepartment of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, ThailandChaiwat BoonkaewwanDepartment of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, ThailandJournal Article20150813<br /><strong>Objective: </strong>Stevioside is a natural non-caloric sweetener which has been reported to have anti-inflammatory activity. The aim of the present study was to examine <em>in vitro </em>and<em> in vivo</em> effects of stevioside on rats plasma levels of tumor necrosis factor- α (TNF-α), interleukin-1β (IL-1β), TNF-α and IL-1β release from lipopolysaccharide(LPS)-stimulated rat peripheral blood mononuclear cells (PBMCs). <br /><br /><strong>Materials and Methods</strong>: Male wistar rats weighing between 170-220 g were given stevioside (0, 500 and 1000 mg/kg BW/day) for 6 weeks. Mononuclear cells were separated from peripheral blood samples. TNF-α and IL-1β levels in plasma and the release of TNF-α and IL-1β from PBMCs were determined using rat enzyme-linked immunosorbent assay (ELISA) kits. <br /><br /><strong>Results:</strong> Plasma levels of TNF-α and IL-1β were found to be non-detectable in control and groups treated with 500 and 1000 mg/kg of stevioside. Regarding TNF-α release from LPS-stimulated PBMCs, rats that were orally fed with 500 and 1000 mg/kg of stevioside were significantly different (p<0.05) from those in LPS-treated control group (186.8<span style="text-decoration: underline;">+</span>18.6 and 151.4 <span style="text-decoration: underline;">+</span> 15.4 vs 248.6<span style="text-decoration: underline;">+</span>21.4 pg/ml). Additionally, IL-1β levels in rats treated with 500 and 1000 mg/kg of stevioside were significantly different (p<0.05) from those in LPS-treated control group (220.0<span style="text-decoration: underline;">+</span>12.1 and 158.1 <span style="text-decoration: underline;">+</span> 22.6 vs 294.4<span style="text-decoration: underline;">+</span>16.1 pg/ml). <br /><br /><strong>Conclusion:</strong> Consumption of stevioside has an inhibitory effect on the release of TNF-α and IL-1β from LPS-stimulated PBMCs in rats.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79307220170301Cassia fistula: A remedy from Traditional Persian Medicine for treatment of cutaneous lesions of pemphigus vulgaris107115662910.22038/ajp.2016.6629ENFatemh AtarzadehDepartment of Traditional Iranian Medicine, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, IranAmir JaladatDepartment of Traditional Persian Medicine, School of Medicine, Shiraz, IranLadan DastgheibMolecular Dermatology Research Center, Department of Dermatology, Shiraz University of Medical Sciences, Shiraz, Iran0000000221179375Gholamreza AminDepartment of Traditional Pharmacy, Tehran University of Medical Sciences, Tehran, IranMajid NimrouziDepartment of Traditional Persian Medicine, School of Medicine, Shiraz, Iran0000-0003-4630-2082Mohammad KamalinejadDepartment of Pharmacognosy, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran0009-0006-6373-1320Journal Article20150810<br /><strong>Objective: </strong>Pemphigus is a rare autoimmune disease that may be fatal without proper medical intervention. It is a blistering disease that involves both the skin and mucus membranes, in which the most important causes of death comprise superimposed opportunistic infections and complications of long-term high-dose corticosteroid therapy or prolonged consumption of immune suppressant drugs. Skin lesions are the most important sources of infection, and any local treatment decreasing the healing time of lesions and reducing the total dosage of drugs is favorable. <br /><strong>Materials and Methods</strong>: Here, we review the probable mechanism of action of a traditional formulary of <em>Cassia fistula</em> (<em>C. fistula</em>) fruit extract in almond oil as a new topical medication for reducing the duration of treatment of pemphigus vulgaris erosions. <br /><strong>Result</strong>: <em>C. fistula </em>fruit oil has lupeol, anthraquinone compounds as rhein and flavonoids. <br />Previous <em>in vitro</em> and animal studies on <em>C. fistula</em> fruit have demonstrated wound healing, antioxidative, anti-leukotrienes, anti-inflammatory, antibacterial and antifungal effects of this plant. <br /><strong>Conclusion</strong>: It is hypothesized that <em>C. fistula</em> L. can be a botanical therapeutic choice for treatment of pemphigus erosions.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79307220170301Preventive effect of Coriandrum sativum on neuronal damages in pentylentetrazole-induced seizure in rats116128775710.22038/ajp.2016.7757ENMojtaba PourzakiDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranMansour HomayounPharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran0000-0003-1676-3443Saeed SadeghiDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranMasoumeh SeghatoleslamDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Microanatomy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Mahmoud HosseiniNeurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran0000-0001-6425-5123Alireza Ebrahimzadeh BideskanDepartment of Anatomy and Cell Biology, Microanatomy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran0000-0003-4333-250XJournal Article20160421<br /><strong>Objective:</strong><em>Coriandrum sativum</em> (<em>C. sativum</em>) as a medicinal plant has been pointed to have analgesic, hypnotic and anti-oxidant effects. In the current study, a possible preventive effect of the hydro-alcoholic extract of the plant on neuronal damages was examined in pentylenetetrazole (PTZ) rat model of seizure.<br /><br /><strong>Materials and Methods</strong><strong>:</strong> Forty male rats were divided into five main groups and treated by (1) saline, (2) PTZ: 100 mg/kg PTZ (i.p) and (3-5) 50, 100 and 200 mg/kg of hydro-alcoholic extract of <em>C. sativum </em>during seven consecutive days before PTZ injection. After electrocorticography (ECoG), the brains were removed to use for histological examination.<br /><br /><strong>Results</strong>: All doses of the extract reduced duration, frequency and amplitude of the burst discharges while prolonged the latency of the seizure attacks (p<0.05, p<0.01, and p<0.001). Administration of all 3 doses of the extract significantly prevented from production of dark neurons (p<0.01, and p<0.001) and apoptotic cells (p<0.05, p<0.01, and p<0.001) in different areas of the hippocampus compared to PTZ group.<br /><br /><strong>Conclusion</strong>: The results of this study allow us to conclude that <em>C. sativum</em>,because of its antioxidant properties, prevents from neuronal damages in PTZ rat model of seizure.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79307220170301A comparison of the efficacy of metronidazole vaginal gel and Myrtus (Myrtus communis) extract combination and metronidazole vaginal gel alone in the treatment of recurrent bacterial vaginosis129136728610.22038/ajp.2016.7286ENMansoure MasoudiMedical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, IranMahmoud KopaeiRafieianMedicinal Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran0000-0003-3190-7863Sepide MirajMedical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, IranJournal Article20151008<br /><strong>Objective: </strong>Due to the high incidence of bacterial vaginosis (BV) and its resistance to chemical medications and considering the anti-bacterial and anti-fungal effects of <em>Myrtus communis, </em>the present study aimed to compare the therapeutic effects of the vaginal gel of <em>M. communis </em>2% (in metronidazole base) with metronidazole vaginal gel 0.75% alone on BV.<br /><br /><strong>Materials and Methods</strong>: This research was a randomized controlled clinical trial conducted on 80 women of 18-40 years old with BV. Patients were divided into two groups of 40 women. Diagnostic criteria were Amsel's criteria and Gram staining. The first group received vaginal gel of metronidazole plus <em>M. communis </em>2% and the second group received metronidazole vaginal gel alone for five consecutive nights. Therapeutic effects and Amsel’s criteria were assessed after one week. Finally, the data were analyzed by SPSS 16 using <em>t-test</em> and Chi square tests.<br /><br /><strong>Results</strong>: There was a significant difference in the therapeutic response between the two groups. The results demonstrated that the combination of metronidazole and <em>M. communis</em> had a higher efficiency (p<0.05). The patients receiving <em>M. communis</em> in metronidazole gel base did not experience any recurrent BV, but 30% of patients taking metronidazole alone faced recurrent BV after three weeks of follow up.<br /><strong>Conclusio</strong>n: Findings of the study suggested that adding <em>M. communis</em> extract to metronidazole increases the efficiency of BV treatment.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79307220170301Kelussia odoratissima potentiates cytotoxic effects of radiation in HeLa cancer cell line137144775910.22038/ajp.2016.7759ENAzar HosseiniPharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran0000-0002-3423-0539Shima Saeidi JavadiDepartment of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranAzar Fani PakdelDepartment of Oncology, Omid Hospitsal, Mashhad, IranSeyed Hadi MousaviDepartment of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran0000-0002-6467-7307Journal Article20160114<br /><strong>Objective: </strong>Cervical cancer is the second most common cause of death from cancer in women throughout the world. The aim of this study was to evaluate the cytotoxic activity of <em>Kelussia odoratissima</em> (<em>K. odoratissima</em>) extract associated with radiotherapy in cervical cancer cells (HeLa cell line).<br /><br /><strong>Materials and Methods: </strong>Different concentration of the extract (25-500µg/ml) was tested in HeLa cell lines. Cell cytotoxicity of the extract and the effects of the extract on radiation (2Gy/min)-induced damages were assessed by MTT assay. Apoptosis was assessed using flow cytometric analysis.<br /><br /><strong>Result: </strong><em>K. odoratissima</em> decreased cell viability in HeLa cell line in a concentration and time-dependent manner. When compared to the control,<em>K. odoratissima</em> induced a sub-G1 peak in the flow cytometry histogram of treated cells, indicating that apoptotic cell death is involved in <em>K. odoratissima</em>-induced toxicity. It was also shown that <em>K. odoratissima</em> sensitizes cells to radiation-induced toxicity.<br /><br /><strong>Conclusion</strong>: Our result showed the extract increased the radiation effect. This observation may be related to the presence of active compounds such as phthalides and ferulic acid.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79307220170301Anti-carcinogenic and anti-angiogenic properties of the extracts of Acorus calamus on gastric cancer cells145156748510.22038/ajp.2016.7485ENSamaneh Rahamoz HaghighiDepartment of Plant Breeding, Faculty of New Sciences and Technologies, Graduate University of Advanced Technology, Kerman, IranMalek Hossein AsadiDepartment of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran0000-0003-2333-4938Hassan AkramiDepartment of Biology, Faculty of Basic Science, Razi University, Kermanshah, IranAmin BaghizadehDepartment of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, IranJournal Article20160106<br /><strong>Objective: </strong><em>Acorus calamus</em> (<em>A. calamus</em>) has been used as a medicinal plant in Asia for its effects on digestive system for the last 2000 years. To investigate the anti-cancer activity of rhizome of<em> A. calamus</em>, the ethanolic and methanolic extracts and essential oil of the rhizome were prepared and their effects were assessed on human gastric cancer cell line (AGS). <br /><strong>Materials and Methods: </strong>The viability of cells which were treated with the extracts and the essential oil was assessed by MTT assay. To evaluate the anti-angiogenic property of the extracts, <em>in vitro</em> tube formation assay was done. Cell cycle distribution and the expression of Oct4 and Nucleostemin, after treatments, were checked by flowcytometry and quantitative RT-PCR, respectively. Furthermore, analysis of essential oil from <em>A.calamus</em> was done by GC-MS. <br /><strong>Results:</strong> Our results showed that the growth of AGS cells was inhibited by the extracts and essential oil and the extracts inhibited the angiogenesis in HUVEC cells. Our data revealed that the extracts and essential oil of <em>A. calamus</em> caused G1 arrest in AGS cells and downregulation of Oct4 and NS after treatment. By GC-MS analysis, we found new compoundssuch as epiprezizaene, valencene and isocyclocitral in essential oil of <em>A. </em><br /><strong>Conclusions:</strong> All together, our results showed that the extracts of <em>A. calamus</em> have anti-proliferative and anti-angiogenic effects on cancer cells.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79307220170301Antioxidant, cytotoxic and DNA protective properties of Achillea eriophora DC. and Achillea biebersteinii Afan. extracts: A comparative study157168775610.22038/ajp.2016.7756ENMaryam Varasteh-kojourianDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranParvaneh AbrishamchiDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranMaryam MatinCell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, IranJavad AsiliDepartment of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran0000-0002-4635-474XHamid EjtehadiDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranFatemeh KhosravitabarDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranJournal Article20150924<br /><strong>Objective: </strong><em>Achillea</em> is a traditional medicinal herb which contains different phenol and flavonoid compounds that are responsible for <em>Achillea</em> pharmacological effects. We aimed to determine phenol and flavonoid contents, besides antioxidant activities of different extracts from <em>Achillea eriophoraa </em>(<em>A. eriophora</em>) DC. and <em>Achillea biebersteinii </em>(<em>A. biebersteinii</em>) Afan. (endemic species in Iran) and to investigate their effects on human cells.<br /><br /><strong>Materials and Methods</strong>: <em>Achillea</em> extracts, were prepared by maceration and shaking methods, from different parts (aerial parts, stem, leaves and inflorescence) of two species using methanol and ethanol as solvents. Total phenol and flavonoid contents were measured by spectrophotometry, and antioxidant activities of the extracts were determined by DPPH radical scavenging, BCB and TBARS assays. Cytotoxicity and antioxidant activities of the extracts were investigated in Human Foreskin Fibroblast (HFF3) cells using MTT, comet and H<sub>2</sub>O<sub>2</sub> assays.<br /><br /><strong>Results</strong>: Methanol extracts of <em>A. biebersteinii</em> prepared from leaves and inflorescence by maceration method exhibited maximum phenol (1657.58 ± 36.45 mg GAE/100 g DW) and flavonoid (264.00 ± 62.16 mg QUE/100 g DW) contents. Leaf methanol extract showed significantly higher antioxidant activity (0.0276 ± 0.003, 0.16 ± 0.016 and 13.96 ± 0.26 mg/ml for DPPH, BCB and TBARS IC50s, respectively) than those of the other extracts. Leaf extract of <em>A. biebersteinii</em> was not cytotoxic even at the highest examined dose (512 µg/ml) and inhibited cell toxicity induced by H<sub>2</sub>O<sub>2</sub> (98% viability for the cells pretreated with plant extract in the presence of H<sub>2</sub>O<sub>2</sub>). Comet assay also confirmed high DNA protective activity of leaf extracts.<br /><br /><strong>Conclusion</strong>: <em>Achillea</em> extracts possess remarkable antioxidant activity, and could be good natural alternatives to synthetic antioxidants in pharmaceutical and food industries.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79307220170301Antidiabetic, hypolipidemic and hepatoprotective effects of Arctium lappa root’s hydro-alcoholic extract on nicotinamide-streptozotocin induced type 2 model of diabetes in male mice169179784310.22038/ajp.2016.7843ENAkram AhangarpourHealth Research Institute, Diabetes Research Center, Department of Physiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran0000-0002-9534-9699Hamid HeidariDepartment of Physiology, Health Research Institute, Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Physiology, School of Medicine, Qom University of Medical Sciences, Qom, IranAli Akbar OroojanDepartment of Physiology, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranFarhang MirzavandiDepartment of Nutrition, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranKhalil Nasr EsfehaniDepartment of Nutrition, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranZienab Dehghan MohammadiHealth Research Institute, Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranJournal Article20160606<strong>Objective: </strong><em>Arctium lappa</em> (burdock), <em>(A. lappa)</em> root has hypoglycemic and antioxidative effects, and has been used for treatment of diabetes in tradition medicine. This study was conducted to evaluate the antidiabetic and hypolipidemic properties of <em>A. lappa</em> root extract on nicotinamide-streptozotocin (NA-STZ)-induced type2 diabetes in mice.<br /><strong>Materials and Methods:</strong> In this investigation, 70 adult male NMRI mice (30-35g) randomly divided into 7 groups (n=10) as follow: 1-control, 2-type 2 diabetic mice, 3-diabetic mice that received glibenclamide (0.25 mg/kg) as an anti-diabetic drug, 4, 5, 6 and 7- diabetic and normal animals that were pre-treated with 200 and 300 mg/kg <em>A. lappa</em> root extract, respectively, for 28 days. Diabetes has been induced by intraperitoneal injection of NA and STZ. Finally, the blood sample was taken and insulin, glucose, SGOT, SGPT, alkaline phosphatase, leptin and lipid levels was evaluated.<br /><strong>Results:</strong> Induction of diabetes decreased the level of insulin, leptin and high density lipoprotein (HDL) and increased the level of other lipids, glucose, and hepatic enzymes significantly (p<0.05). Administration of both doses of the extract significantly decreased the level of triglyceride, very low density lipoprotein, glucose and alkaline phosphatase in diabetic mice (p<0.05). Insulin levels increased in animals treated with 200 mg/kg (p<0.05) and HDL and leptin levels increased in animals treated with 300 mg/kg of the extract (p<0.01).<br /><strong>Conclusion:</strong> The results showed that <em>A. lappa</em> root extract, at specific doses, has an anti-diabetic effect through its hypolipidemic and insulinotropic properties. Hence, this plant extract may be beneficial in the treatment of diabetes.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79307220170301Kidney stone formation and antioxidant effects of Cynodon dactylon decoction in male Wistar rats180190784410.22038/ajp.2016.7844ENAlireza GolshanSchool of Medicine, North Khorasan University of Medical sciences, Bojnurd, IranParichehr HayatdavoudiNeurocognitive Research Center, Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranMousa AL-Reza HadjzadehNeurocognitive Research Center, Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran0000-0003-0086-6472Abolfazl Khajavi RadNeurogenic Inflammation Research Center,Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran0000-0001-7046-1134Nema Mohamadian RoshanDepartment of Pathology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhd, IranAbbasali AbbasnezhadDepartment of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad,IranSeyed Mojtaba MousaviDepartment of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad,IranRoghayeh PakdelDepartment of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad,IranBatool ZareiDepartment of physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranAzita AghaeePharmacological Research Center of Medicinal Plants, Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad,IranJournal Article20160302<strong>Objectives</strong>: The antioxidant capacity impairs in kidney and urinary bladder of animals with stone disease. Herbal medicine can improve the antioxidant condition of renal tissue. <em>Cynodon dactylon</em> (<em>C. dactylon</em>) is a medicinal plant with antioxidative and diuretic properties and different preparations of this plant have shown promising effects in stone disease. Assessment of the whole plant decoction to prevent kidney stone disease as well as its antioxidant effects was the aim of this paper.<br /><strong>Materials and Methods:</strong> Fifty male Wistar rats were randomly divided into 5 experimental groups (n=10). One group was left without treatment and four groups received ethylene glycol (1% v/v) in drinking water for 6 weeks. Three doses of <em>Cynodon dactylon</em> aqueous decoction (12.5, 50 and 200 mg/kg BW) were added to the drinking water of groups 3-5. Finally, water intake, 24-hour urine volume, MDA, total thiol concentration and FRAP value were measured in the serum and kidney tissues. The CaOx depositions were evaluated by hematoxylin and eosin staining.<br /><strong>Results:</strong> Compared to the ethylene glycol-treated group, 200 mg/kg <em>C. dactylon</em>, lowered stone incidents, decreased urine volume, increased FRAP/g Cr (43%) and thiol content (p<0.05) with no significant alteration of water intake, MDA decreased significantly compared to <em>C. dactylon</em> 12.5 (p<0.01). Kidney weight increased and body weight decreased in ethylene glycol-treated group compared to the control group (p<0.05).<br /> <strong>Conclusion:</strong> A minimum dose of 200 mg/kg <em>C. dactylon</em> reduced stone formation and simultaneously increased total antioxidant power of serum and preserved MDA content and water.Mashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79307220170301Capparis spinosa L. aqueous extract evokes antidiabetic effect in streptozotocin-induced diabetic mice191198776110.22038/ajp.2016.7761ENMohamed EddouksFaculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, MoroccoAhmed LemhadriFaculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, MoroccoMorad HebiFaculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, MoroccoAhmed EL HidaniFaculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, MoroccoNaoufel Ali ZeggwaghISPITS Rabat, Avenue Hassan II Km 4,5 Route de Casa, Rabat MarocBachir EL BouhaliFaculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, MoroccoLhoussaine HajjiFaculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, MoroccoRemy Burcelin2UMR 5018 CNRS-UPS and IFR 31, Rangueil Hospital, L1 Bldg, BP 84225 Toulouse 31432 Cedex 4, FranceJournal Article20160405<br /><strong>Objective</strong>: As the aqueous extract of <em>Capparis spinosa</em> (CS) possess antidiabetic effect, he present study aims to reveal the possible mechanism of action of CS in diabetic mice.<br /><br /><strong>Materials and Methods</strong>: Both single and repeated oral administrations of aqueous extract of CS were performed in multi-low dose streptozotocin-induced (MLDS) diabetic mice. Euglycemic hyperinsulinemic clamp was used in association with the endogenous glucose production (perfusion rate of 3-<sup>3</sup>H glucose) to evaluate the effect of CS aqueous extract on insulin sensitivity.<br /><br /><strong>Results</strong>: Our study showed that aqueous extract of CS possess a potent hypoglycaemic activity in MLDS diabetic mice. Furthermore, the analysis perfusion of 3-<sup>3</sup>H glucose demonstrated the parallel decrease of basal endogenous glucose production (EGP) with the hypoglycaemic activity. EGP was lower in CS-Treated group when compared to the control group (p<0.001). The euglycemic hyperinsulinemic clamp technique demonstrated that CS treatment improves insulin sensitivity in peripheral tissues.<br /><br /><strong>Conclusion</strong>: We conclude that the antihyperglycemic effet CS is probably due to the inhibition of basal endogenous glucose production and the improvement of insulin sensitivity in MLDS diabetic mice.