2024-03-19T14:19:09Z
https://ajp.mums.ac.ir/?_action=export&rf=summon&issue=993
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
Phytochemical characterization, antimicrobial activity and reducing potential of seed oil, latex, machine oil and presscake of Jatropha curcas
Amit
Sharma
Mayank
Gangwar
Dharmendra
Kumar
Gopal
Nath
Akhoury Sudhir
Sinha
Yamini
Tripathi
Objective: This study aims to evaluate the antimicrobial activity, phytochemical studies and thin layer chromatography analysis of machine oil, hexane extract of seed oil and methanol extract of presscake& latex of Jatropha curcas Linn (family Euphorbiaceae). Materials and Methods: J. curcas extracts were subjected to preliminary qualitative phytochemical screening to detect the major phytochemicals followed by its reducing power and content of phenol and flavonoids in different fractions. Thin layer chromatography was also performed using different solvent systems for the analysis of a number of constituents in the plant extracts. Antimicrobial activity was evaluated by the disc diffusion method, while the minimum inhibitory concentration, minimum bactericidal concentration and minimum fungicidal concentration were calculated by micro dilution method. Results: The methanolic fraction of latex and cake exhibited marked antifungal and antibacterial activities against Gram-positive and Gram-negative bacteria. Phytochemical analysis revealed the presence of alkaloids, saponins, tannins, terpenoids, steroids, glycosides, phenols and flavonoids. Reducing power showed dose dependent increase in concentration compared to standard Quercetin. Furthermore, this study recommended the isolation and separation of bioactive compounds responsible for the antibacterial activity which would be done by using different chromatographic methods such as high-performance liquid chromatography (HPLC), GC-MS etc. Conclusion: The results of the above study suggest that all parts of the plants possess potent antibacterial activity. Hence, it is important to isolate the active principles for further testing of antimicrobial and other biological efficacy.
Reducing power
Antimicrobial
Phytochemical
Thin layer chromatography
Minimum bactericidal concentration
2016
07
01
366
375
https://ajp.mums.ac.ir/article_5913_62ae25cce35dd4a0bc99253017ad800d.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
In vitro protoscolicidal effects of various concentrations of Ziziphora tenuior L. extract at different exposure times
Mojtaba
Shahnazi
Abbas
Azadmehr
Robabeh
Latiffi
Reza
Hajiaghaee
Mehrzad
Saraei
Mahmood
Alipour
Objective: Hydatidosis is one of the most important zoonotic diseases and surgery is still the main treatment for this problem. One of the side effects of hydatid cyst surgery is recurrence, thus, searching and assessment of some new agents such as medicinal plant extracts are very important. In the present study, the scolicidal effect of ethanolic extract of Ziziphora tenuior L. (Z. tenuior) was investigated. Materials and Methods: Protoscolices were aseptically collected from sheep livers containing hydatid cyst and used in the experiments. Z. tenuior extract was used at concentration of 3-100 mg/ml for 10-60 min. Viability of protoscolices was determined by 0.1% eosin staining. Results: Based on our results, Z. tenuior extract at concentration of 10 mg/ml killed all protoscolices after 20 min. However, this medicinal plant at concentration of 25 mg/ml destroyed all protoscolices in a shorter exposure time (10 min). Therefore, the scolicidal activity of the extract at 10 and 25 mg/ml concentrations was considerably effective in lower concentrations and shorter exposure times. Conclusion: The findings of this study showed that the ethanolic extract of Z. tenuior produces high scolicidal activity; it may be used as an appropriate and effective scolicidal agent in hydatidosis surgery. This is the first report on the protoscolicidal activity of Z. tenuior.
Hydatidosis
Surgery
Medicinal plant
Scolicidal
Hydatid Cyst
Ziziphoratenuior L
2016
07
01
376
382
https://ajp.mums.ac.ir/article_5961_9ab9f6472467576921199ddb5909e851.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
Novel delivery system for natural products: Nano-curcumin formulations
Hamid Reza
Rahimi
Reza
Nedaeinia
Alireza
Sepehri Shamloo
Shima
Nikdoust
Reza
Kazemi Oskuee
Objective: Curcumin is extracted from Curcuma longa and regulates the intracellular signal pathways which control the growth of cancerous cell, inflammation, invasion and apoptosis. Curcumin molecules have special intrinsic features that can target the intracellular enzymes, genome (DNA) and messengers (RNA). A wide range of studies have been conducted on the physicochemical traits and pharmacological effects of curcumin on different diseases like cardiovascular diseases, diabetes, cancer, rheumatoid arthritis, Alzheimer’s, inflammatory bowel disease (IBD), and even it has wound healing. Oral bioavailability of curcumin is rather poor, which would certainly put some boundaries in the employment of this drug. Materials and Methods: Bibliographical searches were performed using MEDLINE/ScienceDirect/OVID up to February 2015 using the following keywords (all fields): (“Curcumin” OR “Curcuma longa”) AND [(nanoparticles) OR (Nanomicelles) OR (micro emulsions) OR (liposome) OR (phospholipid). Results: Consequently, for any developments of curcumin in the future, analogues of curcumin that have better bioavailability or substitute formulations are needed crucially. Conclusion: These studies indicated that nanotechnology can formulate curcumin effectively, and this nano-formulated curcumin with a potent ability against various cancer cells, were represented to have better efficacy and bioavailability under in vivo conditions.
Curcumin
Curcumin Nano formulations
Nano micelle
2016
07
01
383
398
https://ajp.mums.ac.ir/article_6187_fa00aef51aa9891d1f1325eaef72a8a9.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
Neuroprotective effects of Withania coagulans root extract on CA1 hippocampus following cerebral ischemia in rats
Maryam
Sarbishegi
Zahra
Heidari
Hamidreza
Mahmoudzadeh- Sagheb
Mohharam
Valizadeh
Mahboobeh
Doostkami
Objective: Oxygen free radicals may be implicated in the pathogenesis of ischemia reperfusion damage. The beneficial effects of antioxidant nutrients, as well as complex plant extracts, on cerebral ischemia-reperfusion injuries are well known. This study was conducted to determine the effects of the hydro-alcoholic root extract of Withania coagulans on CA1 hippocampus oxidative damages following global cerebral ischemia/reperfusion in rat. Materials and Methods: Male Wistar rats were randomly divided in five groups: control, sham operated, Ischemia/ Reperfiusion (IR), and Withania Coagulans Extract (WCE) 500 and 1000mg/kg + I/R groups. Ischemia was induced by ligation of bilateral common carotid arteries for 30 min after 30 days of WCE administration. Three days after, the animals were sacrificed, their brains were fixed for histological analysis (NISSL and TUNEL staining) and some samples were prepared for measurement of malondialdehyde (MDA) level and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activity in hippocampus. Results: WCE showed neuroprotective activity by significant decrease in MDA level and increase in the SOD, CAT and GPx activity in pretreated groups as compared to I/R groups (p<0.001). The number of intact neurons was increased while the number of TUNEL positive neurons in CA1 hippocampal region in pretreated groups were decreased as compared to I/R group (p<0.001). Conclusion: WCE showed potent neuroprotective activity against oxidative stress-induced injuries caused by global cerebral ischemia/ reperfusion in rats probably by radical scavenging and antioxidant activities.
Withania coagulans
Antioxidant Enzymes
Hippocampus
Ischemia
Rat
2016
07
01
399
409
https://ajp.mums.ac.ir/article_6261_0eae4589c4520c6e283ea944c482523a.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines
Mohamad Ali
Geryani
Davood
Mahdian
Seyed Hadi
Mousavi
Azar
Hosseini
Objective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-proliferative and pro-apoptotic effects of flower extract of P. abrotanoides on MCF-7 and Hela cell lines. Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin and 100 µg/ml streptomycin and incubated with different concentrations of plant extracts. Cell viability was quantified by MTT assay. Apoptotic cells were determined using propidium iodide (PI) staining of DNA fragmentation by flow cytometry (sub-G1 peak). Results: P. abrotanoides extract inhibited the growth of malignant cells in a time and dose-dependent manner and 1000 µg/ml of extract following 48h of incubation was the most cytotoxic dose against Hela cell in comparison with other doses; however, in MCF-7 cells,1000 and 500 µg/ml PA induced toxicity at all time points but with different features.. Analysis of flowcytometry histogram of treated cells compared with control cells indicated that the cytotoxic effect is partly due toapoptosis induction. Conclusion: Hydro-alcoholic extract of P. abrotanoides flowers inhibits the growth of MCF-7 and HeLa cell lines, partly via inducing apoptosis. Their inhibitory effect was increased in a time and dose-dependent manner, especially in MCF7 cells. However, further studies are needed to reveal the mechanisms of P. abrotanoides extract-induced cell death.
Perovskia abrotanoides
Breast Cancer
Cervical Cancer
Apoptosis
MCF-7
HeLa
2016
07
01
410
417
https://ajp.mums.ac.ir/article_6263_230bf2d1ce8678f7e5684bf83ca7ee5f.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
Effects of Persian leek (Allium ampeloprasum) on hepatic lipids and the expression of proinflammatory gene in hamsters fed a high-fat/ high-cholesterol diet
Vahideh
Fatoorechi
Marjan
Rismanchi
Javad
Nasrollahzadeh
Objective: Persian leek is one of the most widely used herbal foods among Iranians. In this study, effects of oral administration of Persian leek on plasma and liver lipids were examined in hamster. Materials and Methods: Male Syrian hamsters were randomly divided into three groups: control (standard diet), high fat control (high-fat/high-cholesterol diet), Persian leek (high-fat/high-cholesterol diet + 1% per weight of diet from dried powdered Persian leek) for 14 weeks. Results: High fat diet increased plasma and liver lipids as compared to standard diet. Adding Persian leek to the high-fat/high-cholesterol diet resulted in no significant changes in the concentration of the plasma lipids or liver cholesterol. However, liver triglycerides (TG), plasma Alanine aminotransferase and gene expression of tumor necrosis factor- α were decreased in hamsters fed high-fat diet containing Persian leek as compared to high-fat diet only. Conclusion: Persian leek might be considered as a herbal food that can reduce liver TG accumulation induced by high fat diets.
Leek
High fat
Liver lipids
Hyperlipidemia
2016
07
01
418
424
https://ajp.mums.ac.ir/article_6351_7748482ae108974fe447938a96916a0c.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
Phytochemical screening and evaluation of antioxidant activities of Dracocephalum kotschyi and determination of its luteolin content
Mansureh
Kamali
Susan
Khosroyar
Hossein
Kamali
Tooba
Ahmadzadeh Sani
Ameneh
Mohammadi
Objective: Dracocephalum kotschyi (Lamiaceae family) has been usedin traditional medicine for stomach and liver disorders, headache and congestion. In the present study, we have investigated phytochemical properties and antioxidant activities of dichloromethane, ethyl acetate and methanol extracts of D.kotschyi. Material and Methods: Antioxidant activities of extracts were evaluated using the integration of HPLC-DPPH and ferric reducing antioxidant power (FRAP) methods. In addition, the luteolincontent was determined using HPLC method. Results: The highest antioxidant activity was observed for the methanol extract (among the three tested extracts) showing 50% DPPH scavenging activity at 4.85µg/ml as compared to butylated hydroxy toluene (BHT) and ascorbic acid (3.00 µg/ml, 0.97 µg/ml). Also, luteolin was detected in methanol extract; it was identified by comparing its retention time and DAD spectra with standard and it was one of antioxidant components of this plant. In addition, the antioxidant activity of methanol extract was higher than BHT, in FRAP assay. Total phenolic content was in the range of 11.62-22.29 mg Gallic acid /gram of dry extract and flavonoid content was in the range of 3.97-5.042 mg Quercetin/ gram of extract for dichloromethane, ethyl acetate and methanol extracts. The quantity of luteolin in D.kotschyiwas found to be 1061.005 µg/g of dried plant. Conclusion: The results of this investigation indicated that luteolin plays major role in the antioxidant activity of the plant.
Antioxidant
Dracocephalum kotschyi
Luteolin
HPLC
2016
07
01
425
433
https://ajp.mums.ac.ir/article_6377_c1cecac2bf89b960665aa0e4382355aa.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
Protective effect of Viola tricolor and Viola odorata extracts on serum/glucose deprivation-induced neurotoxicity: role of reactive oxygen species
Seyed Hadi
Mousavi
Behnaz
Naghizade
Solmaz
Pourgonabadi
Ahmad
Ghorbani
Objective: Oxidative stress plays a key role in the pathophysiology of brain ischemia and neurodegenerative disorders.Previous studies indicated that Viola tricolor and Viola odorataare rich sources of antioxidants. This study aimed to determine whether these plants protect neurons against serum/glucose deprivation (SGD)-induced cell death in an in vitro model of ischemia and neurodegeneration. Methods and Material: The PC12 neuronal cells were pretreated for 4 hr with 1 to 50 µg/ml of V. odorata or V. tricolor hydroalcoholic extracts followed by 24 hr incubation under SGD condition. Cell viability was evaluated by 4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2H-tetrazolium bromide assay. The level of intracellular reactive oxygen species (ROS) was quantitated by flow cytometry using 2',7'- dichlorofluorescin diacetate as a probe. Results: SGD condition led to significant decrease in cell viability (p < 0.001). Pretreatment with both V. tricolor and V. odorata extracts reduced the SGD-induced cytotoxicity. SGD resulted in a significant increase in intracellular ROS production (p < 0.001). Both extracts at concentrations of 25 and 50 µg/ml could reverse the increased ROS production (p < 0.05). Conclusion: Results of the present study showed that V. tricolor and V. odorata protect neuronal cells against SGD-induced cell death, at least in part, by their antioxidant activities. Further studies on the possible application of these plants in prevention or treatment of cerebral ischemia and neurodegenerative diseases seem to be warranted.
PC12
Reactive Oxygen Species
Viola tricolor
Viola odorata
2016
07
01
434
441
https://ajp.mums.ac.ir/article_6327_a56afacd18d062f7e9e5eab94bfd931c.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
The effects of crocin and safranal on the yawning induced by intracerebroventricular injection of histamine in rats
Mina
Taati
Amir
Erfanparast
Esmaeal
Tamaddonfard
Hamid
Ghasemi
Objective: Crocin and safranal, as the major constituents of saffron, have many biological activities. This study investigated the effects of crocin and safranal on yawning response induced by intracerebroventricular (i.c.v.) injection of histamine in rats. Materials and Methods: In ketamine/xylazine-anesthetized rats, a guide cannula was implanted in the right ventricle of the brain and yawning induced by i.c.v. injection of histamine. Crocin and safranal were intraperitoneally (i.p.) injected alone and before i.c.v. injection of histamine. Results: Histamine at the doses of 10 and 20 µg/rat produced yawning. Mepyramine (a histamine H1 receptor antagonist) 40 µg/rat significantly (p<0.05) prevented histamine (20 µg/rat)-induced yawning. Crocin (30 mg/kg) and safranal (1 mg/kg) significantly (p<0.05) increased histamine (10 µg/rat)-induced yawning. Crocin and safranal also induced yawning when injected before mepyramine plus histamine administration. Conclusion: The results of the present study showed a yawning-inducing effect for central histamine, which was inhibited by mepyramine. Crocin and safranal increased histamine-induced yawning, and also produced yawning when the histamine action is blocked.
Crocin
Histamine
Safranal
Yawning
2016
07
01
442
448
https://ajp.mums.ac.ir/article_6400_1795218df9706173f7d288252309be2c.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
Hydroalcoholic extract of needles of Pinus eldarica enhances pentobarbital-induced sleep: possible involvement of GABAergic system
Fatemeh
Forouzanfar
Ahmad
Ghorbani
Hasan
Rakhshandeh
Mahmoud
Hosseini
Objective: Insomnia is accompanied by several health complications and the currently used soporific drugs can induce several side effects such as psychomotor impairment, amnesia, and tolerance. The present study was planned to investigate the sleep prolonging effect of Pinus eldarica. Materials and Methods: Hydroalcoholic extract (HAE) of P. eldarica, its water fraction (WF), ethyl acetate fraction (EAF) and n-butanol fraction (NBF) were injected (intraperitoneally) to mice 30 min before administration of pentobarbital. Then, the latent period and continuous sleeping time were recorded. Also, LD50 of P. eldarica extract was determined and the possible neurotoxicity of the extract was tested on neural PC12 cells. Results: The HAE and NBF decreased the latency of sleep (p<0.05) and significantly increased duration of sleep (p<0.05) induced by pentobarbital. These effects of P. eldarica were reversed by flumazenil. The LD50 value for HAE was found to be 4.8 g/Kg. HAE and its fractions did not show neurotoxic effects in cultured PC12-cell line. Conclusion: The present data indicate that P. eldarica potentiated pentobarbital hypnosis without major toxic effect. Most probably, the main components responsible for this effect are non-polar agents which are found in NBF of this plant.
insomnia
Diazepam
Pinuseldarica
Sleep
PC12
2016
07
01
449
457
https://ajp.mums.ac.ir/article_6451_901d9c6fef738f7f7d78a95c05e75cff.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
The effect of Allium sativum (Garlic) extract on infectious bronchitis virus in specific pathogen free embryonic egg
Tabassom
Mohajer Shojaei
Arash
Ghalyanchilangeroudi
Vahid
Karimi
Abbas
Barin
Naser
Sadri
Objective: Garlic is a plant has been used as a flavor, and anti-microbial and anti-diarrheal agent. Infectious bronchitis virus (IBV) is a coronavirus. The available vaccines against IBV cannot cover new variants. This study evaluated the inhibitory effects of garlic extract on IBV. Materials and Methods: The constituents of garlic extract were detected by gas chromatography. This study was done in four groups of embryonic SPF eggs; first group was used for virus titration; second group received the mixture of different virus titration and constant amount of garlic extract; third group received 10-3 titration of virus and after 8 hr received garlic extract and the last group received different dilutions of garlic extract. Results: Based on our results, in the second group, IBV vaccine strain (4/91) at all titration and M41 in 10-2 and 10-3 titration and in the third group both variants of virus the embryonic Index (EI) was significantly increased. Conclusion: The garlic extract had inhibitory effects on IBV in the chickens embryo.
Infectious Bronchitis Virus
Garlic extract
Embryonic Index
Herbal Medicine
Antiviral
2016
07
01
458
467
https://ajp.mums.ac.ir/article_6455_b12ce3bc382a2e65f28416324779d1d6.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
Therapeutic effect of frankincense in a rat model of Alzheimer’s disease
Siamak
Beheshti
Rezvan
Aghaie
Objective: Frankincense improves memory in different models of learning. However, its influence on models of Alzheimer's disease (AD) has not been studied widely. In the present study, the therapeutic effect of frankincense was evaluated in a model of AD induced by i.c.v administration of streptozotocin. Materials and Methods: Under stereotaxic surgery, two guide cannulas were implanted in the lateral ventricles of adult male Wistar rats weighing 230-270 g. One group received streptozotocin (1.5 mg/kg/2μl/side) bilaterally on the first and third day of surgery. Another group received artificial cerebro-spinal fluid. Fourteen days after surgery, learning was evaluated using the passive avoidance paradigm. Four other groups of animals received frankincense (50 mg/kg) or its solvent after establishment of AD for 21 or 42 consecutive days, and then, memory retrieval was assessed. Results: Streptozotocin increased the number of stimulations required for induction of short-term memory and decreased step-through latency on the test day, significantly (p<0.05). Chronic injection of the aqueous extract of frankincense for 21 days did not affect learning parameters, but injection of it for 42 days, significantly increased step-through latency (p<0.05), decreased the number of step-through into the dark compartment (p<0.01) and decreased the time spent in the dark compartment (p<0.05). Conclusion: The results indicate that chronic administration of frankincense has the potential to improve dementia type of AD induced by i.c.v injection of streptozotocin in a time-dependent manner.
Streptozotocin
Alzheimer’s disease
Frankincense
Memory
Rat
2016
07
01
468
475
https://ajp.mums.ac.ir/article_6465_6c970c3e7339e3c5fd66254fc7b7cba1.pdf
Avicenna Journal of Phytomedicine
2228-7930
2228-7930
2016
6
4
Anxiolytic-like effect of ethanolic extract of Argemone mexicana and its alkaloids in Wistar rats
Aideé Itzel
Arcos-Martínez
Omar David
Muñoz-Muñiz
Miguel Ángel
Domínguez-Ortiz
Margarita Virginia
Saavedra-Vélez
Maribel
Maribel Vázquez-Hernández
Maria Gabriela
Alcantara-Lopez
Objective: Argemone mexicana is a Papaveracea plant; some reports have shown their antibacterial, anti-cancer, sedative and probably anti-anxiety properties. From their aerial parts, flavonoids and alkaloids have been isolated, which are intrinsically related to some actions on the central nervous system. The aim of this study was to evaluate the anxiolytic-like effects of the plant, using its ethanolic extract and alkaloid-enriched extract obtained from fresh leaves. Material and Methods: Phytochemical screening was carried out together with evaluation of antioxidant capacity and the enrichment of alkaloids present in the extract. Subsequently, 100 and 200 mg/kg doses of ethanolic extract and alkaloid-enriched extract (200 µg/kg) were intraperitoneally administered to female Wistar rats, which were exposed to elevated plus maze (EPM) test. Picrotoxin (1 mg/kg), a non-competitive gamma-aminobutyric acid A (GABAA) chloride channel antagonist, was used in experimental procedures to evaluate if this receptor is involved in the anxiolytic-like effects of A. mexicana. To discard motor effects associated with the treatments, the rats were evaluated by the locomotor activity test. Results: Only the ethanolic extract at 200 mg/kg and alkaloid-enriched extract (200 µg/kg) produced anxiolytic-like effects similarly to diazepam 2 mg/kg on EPM test, without affecting locomotor activity. Meanwhile, the administration of picrotoxin blocked anti-anxiety effect of alkaloid-enriched extract of the plant. Conclusion: These results showed that A. mexicana is a potential anxiolytic agent and we suggest that this effect is mediated by the GABAA receptor. These effects are related to the presence of alkaloids.
Argemone mexicana
Alkaloids
GABAA receptor antagonist
Anxiolytic-like effect
2016
07
01
476
488
https://ajp.mums.ac.ir/article_6701_d5ecdcfdabed6e1f87ea70f49bf6a7ca.pdf