TY - JOUR ID - 15662 TI - In vitro and in vivo activity of the essential oil and nanoemulsion of Cymbopogon flexuosus against Trichomonas gallinae JO - Avicenna Journal of Phytomedicine JA - AJP LA - en SN - 2228-7930 AU - Baccega, Bruna AU - Islabão, Yan AU - Mello, Alexia Brauner de AU - Martins, Filipe Obelar AU - Santos, Carolina Caetano dos AU - Ourique, Aline Ferreira AU - Gundel, Samanta da Silva AU - Macedo, Marcia Raquel Pegoraro de AU - Vianna, Élvia Elena Silveira AU - Farias, Nara Amélia da Rosa AU - Oliveira, Camila Belmonte AD - Department of Microbiology and Parasitology- Federal University of Pelotas, Pelotas, RS, Brazil AD - Laboratory of Nanotechnology - Franciscan University, Santa Maria, RS, Brazil Y1 - 2021 PY - 2021 VL - 11 IS - 1 SP - 32 EP - 43 KW - Phytotherapy KW - Nanotechnology KW - birds KW - protozoa KW - lemon grass DO - 10.22038/ajp.2020.15662 N2 - Objective: This study was done to evaluate the in vitro and in vivo effects of the essential oil (OE-CL) and nanoemulsion (N-CL) of Cymbopogon flexuosus against Trichomonas gallinae.Materials and Methods: In vitro assays were done with 106 parasites and OE-CL and N-CL in the concentrations: 110, 220, 330, 440, 550, 660, 770 and 880 µg/ml and four controls: CN (culture medium and trophozoites), MTZ (trophozoites plus 800 µg/ml of metronidazole), TW (trophozoites plus vehicles used for solubilization of derivatives (0.01% Tween) and NB (blank nanoemulsion 880 µg/ml). The in vivo assay was done in 35 quails (Coturnix coturnix) infected experimentally 4x104 mg/kg,  were divided in   seven groups (n=5): A (control–healthy), B (control infected), C (control TW 0.01%), D (NB 0.88 mg/kg), E (drug MTZ 25 mg/kg, F (OE-CL at 0.55 mg/kg ) and G (N-CL at 0.44 mg/kg), during 7 consecutive days.Results: The in vitro test showed that the OE-CL (550 μg/ml) and N-CL (440 μg/ml) concentrations reduced the trophozoites viability in 100%. In the in vivo test, the treatment with OE-CL was efficient on the 4th treatment day and the N-CL after the 3rd day, and the MTZ in the therapeutic concentration was efficient on the 7th day.Conclusion: It can be observed in this study that the lemon grass has natural potential antitrichomonal activity against T. gallinae in vitro and in vivo. UR - https://ajp.mums.ac.ir/article_15662.html L1 - https://ajp.mums.ac.ir/article_15662_ffa419947e5807cb79060b24e329586c.pdf ER -