TY - JOUR ID - 110 TI - Effect of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets JO - Avicenna Journal of Phytomedicine JA - AJP LA - en SN - 2228-7930 AU - Shafiee-Nick, Reza AU - Parizadeh, Seyyed Mohammad Reza AU - Zokaei, Nona AU - Ghorbani, Ahmad AD - Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran AD - Biochemistry and Nutrition Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran Y1 - 2012 PY - 2012 VL - 2 IS - 4 SP - 206 EP - 211 KW - Ganoderma lucidum KW - Insulin KW - Isolated islets KW - Rat DO - 10.22038/ajp.2012.110 N2 - Objective: Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level.This study was undertaken to investigateits direct effect on the islets of Langerhans. Material and Methods: Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer containing 3 or 10 mM glucose in the presence of hydroalcoholic extract of G. Lucidum (1 mg/ml), 3-isobutyl-1-methylxanthine (IBMX, 100 µM) or vehicle. Results: Exposure of islets to the extract increased insulin secretion at basal (3 mM) glucose concentration. Increase of glucose concentration to 10 mM resulted in a significant increase in the rate of insulin secretion. While the IBMX could augment insulin release evoked by 10 mM glucose, the extract failed to modify it. Conclusion: Our results demonstrate that G. lucidum acts directly on the Langerhans islets to increase basal insulin release.  UR - https://ajp.mums.ac.ir/article_110.html L1 - https://ajp.mums.ac.ir/article_110_43205452fee19c76288153ff9fe01359.pdf ER -