Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
5
2
2015
03
01
The effect of hydro-alcoholic extract of Artemisia absinthium on appetite in male rats
78
83
EN
Sara
Baghban Taraghdari
Biochemistry and Nutrition Research Center and Department of Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.
ddfdfdf@mums.ac.ir
Mohsen
Nematy
Biochemistry and Nutrition Research Center and Department of Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.
mazidim911@mums.ac.ir
Mohsen
Mazidi
Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing, China
fdfdfdf@mums.ac.ir
Maryam
Kamgar
Biochemistry and Nutrition Research Center and Department of Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.
Mohammad
Soukhtanloo
Biochemistry and Nutrition Research Center and Department of Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.
asadasd@mums.ac.ir
Mahmoud
Hosseini
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
sfsfsdfs@mums.ac.ir
Hassan
Rakhshandeh
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
adsfsdf@mums.a.cir
Abdolreza
Norouzy
Biochemistry and Nutrition Research Center and Department of Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran.
dsfdfdsf@mums.a.cir
Habibollah
Esmaily
Health Sciences Research Center, Department of Biostatistics and Epidemiology, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran
saadadad@mums.ac.ir
10.22038/ajp.2015.3513
Objectives: weight loss as a consecution of losing appetite in post-operative patients and those suffering from HIV, cancer, cachexia and inflammatory diseases are the main inducements of morbidity and mortality. There is an increasing demand for more efficacious and endurable appetite stimulating treatment for patients with cachexia. Health economics is influenced by the malnutrition which was accounted for 5% of Iranian populations in 2011. <em>Artemisia absinthium</em> is known as an orexigenic herb in Iranian traditional medicine. Little evidence is available about its orexigenic effect and mechanism. So, the present study evaluated the possible effect on appetite of hydroalcoholic extract of <em>Artemisia absinthium</em><em>.</em> <br/>Materials and Methods: Thirty male Wistar rats were randomly divided into five groups. Vehicle group received 0.5 ml water per day, control group did not receive anything and other 3 groups received 50, 100 and 150 mg/kg of <em>Artemisia absinthium</em> for 7 days respectively. The daily amount of the food eaten by each rat was measured for 10 consecutive days. The amount of energy intake for each rat was also calculated for 7 days during the intervention. The difference in energy intake was calculated and compared between groups. <br/>Results: The results suggest that there was no significant (p>0.05) differences in energy received before and during intervention between three case groups compared with the control group. The energy intake in 1-2 hours after extract injection in all groups, and energy intake after 24 hours interval in third case group (receiving 150 mg/kg extract) is higher compared to other intervals, but it is not significant (p>0.05). So, it can be stated that there was no significant differences between energy intake of 3 case groups and control group. <br/>Conclusion: <em>Artemisia absinthium</em> had no positive and dose-related effects on appetite of rats. Future studies are needed to evaluate the orexigenic effect of this plant.
Artemisia absinthium,Appetite,Weight loss,Orexigenic effect
https://ajp.mums.ac.ir/article_3513.html
https://ajp.mums.ac.ir/article_3513_d29422e80f877019587a27db97e7bb2f.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
5
2
2015
03
01
Cancer therapy with phytochemicals: evidence from clinical studies
84
97
EN
Ahmad
Ghorbani
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences,
ghorbania@mums.ac.ir
Azar
Hosseini
0000-0002-3423-0539
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences
hoseiniaz@mums.ac.ir
10.22038/ajp.2015.3872
Cancer is still one of the major causes of mortality in both developing and developed countries. At this time, in spite of intensive interventions, a large number of patients have poor prognosis. Therefore, the effort for finding new anticancer agents with better efficacy and lesser side effects has continued. According to the traditional recommendations and experimental studies, numerous medicinal plants have been reported to have anticancer effect. Also antiproliferative, proapoptotic, antimetastatic and antiangiogenic effects of several phytochemicals have been shown with in vitro experiments or animal studies. However, only a small number of them were tested in cancerous patients and limited evidence exists on their clinical effectiveness. Also, regarding some phytochemicals, only beneficial effects on cancer-related symptoms or on quality of life have been reported and no positive results exist on their antitumor actions. In this review we focus on phytochemicals that their beneficial effects on various types of cancer are supported by clinical trials. Based our literature search, curcumin, green tea, resveratrol and Viscum album had satisfactory instances of clinical evidence for supporting their anticancer effects. The main findings on these phytochemicals are summarized and discussed.
Cancer,Phytochemical,Plant,Tumor
https://ajp.mums.ac.ir/article_3872.html
https://ajp.mums.ac.ir/article_3872_5e294c5136a484546facad3dc24c9613.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
5
2
2015
03
01
In Vitro antibacterial and in Vivo cytotoxic activities of Grewia paniculata
98
104
EN
Mahmuda
Nasrin
Department of Pharmacy Jahangirnagar University
munninasrin@yahoo.com
Pritesh
Ranjan Dash
Department of Pharmacy, BRAC University
Mohammad
Shawkat Ali
Department of Pharmacy, BRAC University
profdr.shawkat@gmail.com
10.22038/ajp.2015.3820
Objectives:<em> Grewia paniculata</em> (Family: Malvaceae) has been used to treat inflammation, respiratory disorders and fever. It is additionally employed for other health conditions including colds, diarrhea and as an insecticide in Bangladesh. The aim of the present study was to investigate the antibacterial and cytotoxic activities of different extracts of <em>Grewia paniculata</em>. <br/>Materials and Methods: The antibacterial activity was evaluated against both gram negative and gram positive bacteria using disc diffusion method by determination of the diameter of zone of inhibition. Cytotoxic activity was performed by brine shrimp (<em>Artemia salina</em>) lethality bioassay. <br/>Results: In disc diffusion method, all the natural products (400 μg/disc) showed moderate to potent activity against all the tested bacteria. The ethanol extract of bark (EEB) and ethanol fraction of bark (EFB) (400 μg/disc) exhibited highest activity against <em>Shigella dysenteriae</em> with a zone of inhibition of 23±1.63 mm and <span style="text-decoration: underline;"> </span>23±1.77 mm respectively. In the brine shrimp lethality bioassay all the extracts showed moderate cytotoxic activity when compared with the standard drug vincristin sulphate. For example, LC50 value of the ethanol fraction of bark (EFB) was 3.01 μg/ml while the LC50 of vincristine sulphate was 0.52 μg/ml. <br/>Conclusions: The results suggest that all the natural products possess potent antibacterial and moderate cytotoxic.
Grewia paniculata,Malvaceae,Artemia salina,Disc diffusion method
https://ajp.mums.ac.ir/article_3820.html
https://ajp.mums.ac.ir/article_3820_481722dcf0f3d9fd4298c5c180b0f822.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
5
2
2015
03
01
Evaluation of the antibacterial activity of the Althaea officinalis L. leaf extract and its wound healing potency in the rat model of excision wound creation
105
112
EN
Maryam
Rezaei
Researcher, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khoramabad, Iran.
Zeynab
Dadgar
Researcher, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khoramabad, Iran.
z.dadgar67@gmail.com
Ali
Noori-Zadeh
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
alincbc@gmail.com
Seyed Alireza
Mesbah-Namin
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Iraj
Pakzad
Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
Elham
Davodian
Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
10.22038/ajp.2015.3979
Objectives: Wound is defined simply as the disruption of the biochemical, cellular, and anatomic continuity of a tissue. Plants and their extracts known as phytomedicine have immense potential for the management and treatment of wounds. <br/>Materials and Methods: Due to the undesirable side effects, in the control and treatment of the wound infections, it is recommended to use natural materials such as phytochemicals instead of chemically synthesized drugs. Thus, the aim of this research was to study the anti-microbial and wound healing potential of <em>Althaea officinalis L. </em>hydroalchoholic extract in comparison with ciprofloxacin, gentamicin, and penicillin antibiotics on clinical strains as well as pathogenic bacteria such as <em>Pseudomonas aeruginosa</em>, <em>Escherichia coli</em>, <em>Staphylococcus aureus</em>, and <em>Listeria monocytogenes</em> under in vitro conditions using micro broth dilution and disc diffusion methods. Moreover, MIC and MBC of its hydroalchoholic extract was also evaluated. <br/>Results: The results showed that although <em>Althaea officinalis L.</em> extract was not effective on gram-negative bacteria but it was efficacious on gram-positive bacteria. The extract was also tested in the form of topical administration on excision wound model in rats. In the extract-treated wounds, the wound healing percent was significantly increased in comparison with controls. <br/>Conclusions: Based on this research, herbal extract of <em>officinalis L.</em> can be a great candidate for the treatment of gram-positive infections and merits further studies.
Anti-bacterial,Wound healing,Althaea officinalis,Phytochemicals
https://ajp.mums.ac.ir/article_3979.html
https://ajp.mums.ac.ir/article_3979_03abff69872b46b4bef549f716ce092f.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
5
2
2015
03
01
Effects of aqueous extract of celery (Apium graveolens L.) leaves on spermatogenesis in healthy male rats
113
119
EN
Ameneh
Hardani
Department of Public Health, Student at the Faculty of Hygiene Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
aa_hardani@yahoo.com
Mohammad Reza
Afzalzadeh
Department of Physiology, Student at the Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
reza20afzal@hotmail.com
Ashraf
Amirzargar
Department of Physiology and Diabetes Research Center, Faculty of Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
amirzargar57@yahoo.com
Esrafil
Mansouri
Department of Histology Research Center, Faculty of Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
emansori@yahoo.com
Zakieh
Meamar
Department of engineering Environmental Health, Faculty of Hygiene Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
zaki-m@yahoo.com
10.22038/ajp.2015.3948
Objectives: Nowadays, a lot of attention has been paid to the therapeutic properties of herbs, including evaluation of the effects of these plants on fertility in laboratory animals. <em>Apium graveolens</em> L. (celery) has been widely used in traditional medicine for treatment of various disorders including impotency. Therefore, this study was designed to investigate the effects of aqueous extract of <em>A. graveolens</em> on testicular tissue and spermatogenesis in healthy male rats. <br/>Materials and Methods: In this research, 24 apparently healthy male rats were divided into three groups, including eight rats in each. The first group as control received only distilled water 1 ml/animal/day. The second and third groups orally received 100 and 200 mg/kg b.w. of the extract, respectively, for 30 days. The day after the last administration of the extract, the rats were sacrificed, the testes were removed entirely, and the morphometric studies were carried out. Epididymal sperm count and histological studies of testicular tissue were conducted. <br/>Results: The comparison between the treated and control groups revealed a remarkable increase in the seminiferous tubules diameter, testes volume (p≤0.001), and the number of spermatogonia, primary spermatocytes and spermatozoa. Furthermore, the increase in the number of spermatids and epididymal weight were only significant at high doses of the extract (p≤ 0.05). <br/>Conclusions: The results from this study indicated that administration of celery leaf extract may improve spermatogenesis process and also be useful for some sperm fertility parameters.
Celery,Leaves,Testes,Spermatozoid,Spermatogenesis,Rat
https://ajp.mums.ac.ir/article_3948.html
https://ajp.mums.ac.ir/article_3948_6d49ea5a0bd94ca135d4015f96a850b9.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
5
2
2015
03
01
Ellagic acid improved arrhythmias induced by CaCL2 in the rat stress model
120
127
EN
Mahin
Dianat
0000-0002-0305-5715
Physiology Research Center and Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
dianat@ajums.ac.ir
Negin
Amini
Physiology Research Center and Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
negin.aminii@yahoo.com
Mohammad
Badavi
0000-0003-2290-8565
Physiology Research Center and Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
badavim@yahoo.com
Yaghoub
Farbood
0000-0002-6390-0079
Physiology Research Center and Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
farbood-y@ajums.ac.ir
10.22038/ajp.2015.3871
Objective: In ventricular arrhythmias, due to their free radical scavenging action, antioxidant agents are usually used in the treatment of cardiovascular disease. Since stress is considered as risk factor for increased mortality by causing malignant arrhythmias, the study was designed to evaluate the cardioprotective effects of ellagic acid (EA) on CaCl<sub>2</sub>-induced arrhythmias in rat stress model. <br/>Materials and Methods: Male Sprague-Dawley rats (200-250 g) were divided into four groups: Group I: Control rats (2 ml of saline by gavage), Group II: Rats treated with EA (15 mg/kg, gavage), Group III: stress group, Group IV: received EA plus stress. Stress was applied in a restrainer box (6 hour/day, 21 days). After induction of anesthesia, lead II electrocardiogram was recorded for calculating heart rate and QRS complex. The arrhythmia was produced by injection of CaCl<sub>2</sub> solution (140 mg/kg, iv) and incidences of Ventricular fibrillation, Ventricular premature beats and Ventricular tachycardia were recorded. Results were analyzed by using one-way ANOVA and Fisher`s exact test. pResults: The results showed a positive inotropic effect and negative chronotropic effect for the EA group in comparison with the control group. Incidence rates (%) of premature beats, ventricular fibrillation and ventricular tachycardia in stress group and all the arrhythmia parameters decreased in groups which received EA. <br/>Conclusions: By decreasing the incidence rates of premature beats, fibrillation and ventricular tachycardia in groups which received EA, ellagic acid probably acted as an anti-arrhythmic agent which showed to have aprotective functionin heart.
Ellagic acid,Arrhythmia,Inotropic,Chronotropic,stress,Rat
https://ajp.mums.ac.ir/article_3871.html
https://ajp.mums.ac.ir/article_3871_22353a4359135976f2b9358cae199498.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
5
2
2015
03
01
Antidiarrheal activity and acute oral toxicity of Mentha longifolia L. essential oil
128
137
EN
Ghader
Jalilzadeh-Amin
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
g.jalilzadeh@urmia.ac.ir
Massoud
Maham
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
m.maham@urmia.ac.ir
10.22038/ajp.2015.3937
Objectives: <em>Mentha longifolia</em> L. (<em>Lamiaceae</em>) is an annual herb that is used in the Iranian traditional medicine for treating stomach and intestinal disorders. The purpose of this study was to determine the protective effect of <em>M. longifolia</em> on experimental diarrhea in a rat model. <br/>Materials and Methods: The antidiarrheal activity of essential oil of <em>M. longifolia</em> (20-80 mg/kg) was investigated against castor oil-induced diarrhea in rats using loperamide as the standard reference drug. In acute toxicity evaluation, rats were orally administrated with single dose of EOML at doses ranging from 10 to 1000 mg/kg. <br/>Results: EOML caused a significant (<em>p</em><0.05) and dose-dependent decrease of gastrointestinal transit, nevertheless, it could not block the inhibitory effect of atropine (0.1 mg/kg). EOML at oral doses of 20 and 80 mg/kg protected the animals against castor oil-induced diarrhea significantly (<em>p</em><0.05). EOML decreased the intestinal fluid accumulation as indicated by the significantly (<em>p</em>p<0.001) decrease compared to control. The oral LD<sub>50</sub> of EOML was found to be 470 mg/kg in rat. <br/>Conclusion: Since the inhibition of intestinal hyperactivity and hypersecretory are the bases of the treatment of diarrhea, results obtained in the present study suggest that EOML is endowed with antidiarrheal activity. EOML is moderately toxic for oral medication
Intestinal transit,Diarrhea,Antispasmodic,Essential oil,Castor oil,LD50
https://ajp.mums.ac.ir/article_3937.html
https://ajp.mums.ac.ir/article_3937_5af51255ef6057cd4d8e901ae677b3b9.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
5
2
2015
03
01
Effects of ethanol extract of Salvia hydrangea on hepatic and renal functions of streptozotocin-induced diabetic rats
138
147
EN
Ali
Zarei
Department of Biology, Unit of Science and Research, Islamic Azad University of Damghan, Damghan, I.R. Iran
Gholamhassan
Vaezi
Department of Biology, Unit of Science and Research, Islamic Azad University of Semnan, Semnan, I.R. Iran
gn-vaezi@yahoo.com
Ali Akbar
Malekirad
Department of Biology, Payame Noor University, I. R. Iran
Mohammad
Abdollahi
Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, I. R. Iran
mohammad@tums.ac.ir
10.22038/ajp.2015.3941
Objectives: A wide range of liver and kidney disorders are associated with diabetes and there is a mutual relationship between diabetes and these diseases. Herbal medicine with having abundant ingredients is one of these options. The goal of this study was to compare the effects of alcoholic extract of aerial parts of <em>S</em><em>alvia hydrangea</em> with glibenclamide on functional tests of liver and kidney in diabetic rats induced by streptozotocin. <br/>Materials and Methods: In this study, 35 male Wistar rats were divided into five groups (n= 7 in each group): control, diabetic control, and three experimental diabetic groups. The controls had normal access to water and food, the diabetic control group was given drug solvent and the three experimental groups received ethanol extract of <em>Salvia hydrangea</em> at doses of 100 and 200 mg and glibenclamideat a dose of 10 mg/kg/BW by gavage, respectively. To induce diabetes, a single dose of streptozotocin (60 mg/kg/BW) was injected to rats intraperitoneally. Blood samples were collected at day 21 from all groups and the related blood factors were measured and analyzed. <br/>Results: The results showed that the levels of creatinine, urea, aspartate aminotransferase (AST), and fasting blood sugar (FBS) in all diabetic groups increased compared to the control group. In all experimental groups and the group which received glibenclamide,a significant decrease was shown compared to the diabetic group (<em>p<0.05</em>). <br/>Conclusion: The consumption of alcoholic extract of aerial parts of <em>Salvia hydrangea </em>may have beneficial effects on the reduction of diabetic complications by lowering blood sugar without any adverse effects on the kidney and liver tissue.
Diabetes,Liver,Kidney,Streptozotocin,glibenclamide,Salvia hydrangea
https://ajp.mums.ac.ir/article_3941.html
https://ajp.mums.ac.ir/article_3941_6e39e4aa7d2c3404a8c0ba4bfa83ea94.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
5
2
2015
03
01
Effects of aqueous extract of turnip leaf (Brassica rapa) in alloxan-induced diabetic rats
148
156
EN
Mohammad
Hassanpour Fard
Department of Physiology and Pharmacology, Birjand University of Medical Sciences (BUMS), Birjand, Iran
mhassanpur61@yahoo.com
Ghodratollah
Naseh
Department of General Surgery, BUMS, Birjand ,Iran
moh77@yahoo.com
Nassim
Lotfi
Department of Anatomy, BUMS, Birjand, Iran
lotfi_nassim@yahoo.com
Seyed Mahmoud
Hosseini
Department of Biostatistics, BUMS, Birjand, Iran
mhdpoh@yahoo.com
Mehran
Hosseini
0000-0002-6793-2035
Department of Public Health, Research Centre of Experimental Medicine, BUMS, Birjand, Iran
mehranhosseiny@yahoo.co.in
10.22038/ajp.2015.3980
Objectives: Turnip leaf has been used in folk medicine of Iran for the treatment of diabetes. However,so far no scientific study has been done to support its use in traditional medicine. The present study was carried out to evaluate the possible hypoglycemic efficacy of aqueous extract of turnip leaf (AETL) in diabetic rats. <br/>Materials and Methods: Alloxan-induced diabetic rats were orally treated with AETL at doses of 200 and 400 mg/kg body weight (bw) per day for 28 days. In order to evaluate the anti-diabetic activity, fasting blood glucose concentrations were determined on the 1<sup>st</sup>, 14<sup>th</sup> and 29<sup>th</sup> days. Moreover,at the end of the study, plasma concentrations of total cholesterol, triglyceride (TG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c), aspartate amino transfarase (AST), and alanine amino transferase (ALT) were measured by the use of standard kits and auto-analyzer. <br/>Results:Both doses of AETL significantly decreased (<em>p</em><0.001) blood glucose and ALT levels in diabetic rats after 28 days of administration. AETL at both doses decreased (<em>p</em><0.05) plasma total cholesterol and LDL-c in diabetic rats, but they significantly decreased (p<0.05) HDL-c and increased triglycerideand AST levels in a-dose dependent manner. <br/>Conclusion: The results showed that AETL has a dose- dependent decrease in the blood glucose in diabetic rats. However,we should not be unaware of adverse effects of AETL on lipid profiles and liver enzymes activity, especially decrease of HDL and increase of TG and AST.
Diabetes Mellitus,Alloxan,Brassica rapa
https://ajp.mums.ac.ir/article_3980.html
https://ajp.mums.ac.ir/article_3980_21c551d726bfd6f89cd79bcb23d8c900.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
5
2
2015
03
01
Cytoprotective and antioxidant effects of Echium amoenum anthocyanin-rich extract in human endothelial cells (HUVECs)
157
166
EN
Leila
Safaeian
Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
leila_safaeian@pharm.mui.ac.ir
Shaghayegh
Haghjoo Javanmard
Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
shaghayeghhaghjoo@yahoo.com
Mustafa
Ghanadian
Department of Pharmacognosy, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
ghanadian@pharm.mui.ac.ir
Sima
Seifabadi
Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
simaseyfabadi@yahoo.com
10.22038/ajp.2015.3947
Objective:<em> Echium amoenum </em>Fisch. & C.A. Mey. is used for the treatment of various diseases in traditional medicine. This plant is a major source of anthocyanins with beneficial cardiovascular properties such as anti-atherosclerotic and antihypertensive effects. In the present study, the protective and antioxidant effects of anthocyanin-rich <em>E. amoenum</em> extract were evaluated on human vascular endothelial cells (HUVECs) under oxidative stress. <br/>Materials and Methods: Cell viability and oxidative status were assessed on H<sub>2</sub>O<sub>2</sub>-induced oxidative stress (0.5 mM H<sub>2</sub>O<sub>2 </sub>for 2 h) in HUVECs pretreated by anthocyanin-rich extract from the petals of <em>E. amoenum </em>(25-1000 µg/ml). Cytoprotective effect of the extract was evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The hydroperoxides concentration and ferric reducing antioxidant power (FRAP) were assessed in intra- and extra-cellular fluid of pretreated cells. <br/>Results: Pretreatment of HUVECs with <em>E. amoenum</em> extract at the concentrations of 100-1000 µg/ml reduced the cell death resulted from the exposure to H<sub>2</sub>O<sub>2 </sub>in a concentration-dependent manner. <em>E. amoenum</em> extract decreased hydroperoxides concentration and increased FRAP value in both intra- and extra-cellular fluid at different concentration ranges. Moreover, it did not show cytotoxic effects at the concentration range of 25-1000 µg/ml. <br/>Conclusion: These results suggest antioxidant and protective effect of anthocyanin-rich extract of the petals of <em>E. amoenum</em> against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in HUVECs. However, further investigations are needed for understanding the detailed mechanisms of cytoprotective effects of this traditional herbal medicine.
Echium amoenum,HUVECs,Oxidative stress,Antioxidant
https://ajp.mums.ac.ir/article_3947.html
https://ajp.mums.ac.ir/article_3947_f823ed656698393b80c23213c2e6902a.pdf