Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
6
2019
11
01
The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death
491
498
EN
Zahra
Tayarani Najjaran
0000-0000-0000-0000
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
tayaraninz@mums.ac.ir
Rezvan
Yazdian
0000-0001-7945-4002
Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
yazdianr921@mums.ac.ir
Elaheh
Amini
Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
aminie@gmail.com
Farzaneh
Salek_Abdollahi
Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
salek_abdollahi@gmail.com
Fatemeh
Arasteh
Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
arastehf@mums.ac.ir
Ahmad
Emami
0000-0003-4298-3132
Department of Traditional Pharmacy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
emamia@mums.ac.ir
10.22038/ajp.2019.13098
<strong>Objective:</strong> Oxidative stress is associated with the pathogenesis of brain ischemia and other neurodegenerative disorders. Previous researches have shown the antioxidant activity of <em>Viola</em> <em>odorata</em> L. In this project, we studied neuro-protective and reactive oxygen species (ROS) scavenging activities of methanol (MeOH) extract and other fractions isolated from <em>V. odorata </em>in PC12 cell line in serum/glucose deprivation (SGD) condition.<br /> <strong>Materials and Methods:</strong> The PC12 neuronal cells were pretreated for 6 hr with MeOH extract and fractions of <em>V. odorata </em>(1 to 25 μg/ml) followed by 24 hr incubation under SGD condition. Cell viability was measured by Alamar Blue<sup>® </sup>assay. The level of ROS was calculated using DCFH-DA. Also, Bax/Bcl-2 protein ratio was analyzed by western blot assay.<br /> <strong>Results:</strong> SGD condition significantly decreased cells viability (p<0.001). Pretreatment with EtOAc (12.5 and 25 µg/ml), BuOH (12, 25, 50 µg/ml) and CH<sub>2</sub>Cl<sub>2 </sub>(1.5 µg/ml) fractions of <em>V. odorata</em> reduced SGD-induced cytotoxicity. MeOH extract could not increase the viability significantly. All four semi polar fractions (EtOAc, BuOH, CH2Cl2 and MeOH) decreased SGD-induced ROS production and changed Bax/Bcl-2 ratio.<br /> <strong>Conclusion:</strong> <em>V. odorata </em>showed promising effects against SGD condition; further mechanistic and clinical studies are warranted before application of <em>V. odorata</em> as a neuro-protective agent.
Viola odorata,Violaceae,PC12 cells,ROS,SGD
https://ajp.mums.ac.ir/article_13098.html
https://ajp.mums.ac.ir/article_13098_346b239ac3095c7c0c1a1b1ff586095f.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
6
2019
11
01
Beneficial effects of pumpkin seed oil as a topical hair growth promoting agent in a mice model
499
504
EN
Valiollah
Hajhashemi
0000-0001-5783-2324
Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan university of Medical Sciences, Isfahan, Iran.
vhajhashemi@gmail.com
Parvin
Rajabi
Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Mahdieh
Mardani
Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan university of Medical Sciences, Isfahan, Iran.
10.22038/ajp.2019.13463
<strong><span style="font-size: medium;">Objective:</span></strong><span style="font-size: medium;"> Pumpkin (<em>Cucurbita pepo</em></span><span style="font-size: medium;"> L.) seed oil mainly consists of saturated and unsaturated fatty acids. Previously, it was reported that oral administration of pumpkin seed oil (PSO) improved hair growth in male pattern alopecia. This study aimed to evaluate hair promoting activity of topical PSO in an animal model.</span><br /> <span style="font-size: medium;"><strong>Materials and Methods:</strong> Male Swiss mice (25-30 g) were used. Dorsal hair of mice (2 x 2.5 cm) was gently removed. Groups were treated as follows: (A) Intact control (did not receive testosterone) (B) Testosterone solution only (5% w/v); (C) Testosterone (5%) + PSO (5%); (D) Testosterone (5%) + PSO (10%) (E) Testosterone (5%) + minoxidil (2%). Application of drugs (100 µl) was done for six days a week, for 3 weeks. Observational and microscopic examinations were performed and results of different groups were compared.</span><br /> <span style="font-size: medium;"><strong>Results:</strong> Topical application of testosterone significantly (p<0.01) prevented hair growth (compared with intact control). PSO (10%) increased hair growth score after 3 weeks and histopathological findings confirmed these results. After 3 weeks of treatment, the percentage of follicles in anagen phase was 95±4.6 and 44.4±15 for intact control and testosterone-only</span><span style="font-size: medium;">treated group, respectively. These percentages for PSO (10%) and minoxidil were 75±5.3 and 91.3±4.4, respectively and they could significantly (p<0.001) reverse the effects of testosterone. </span><br /> <span style="font-size: medium;"><strong>Conclusion:</strong> In conclusion, as topical application of PSO showed hair growth promotion, it might be regarded as a promising alternative for treatment of male pattern alopecia. Also, considering its composition, free fatty acids and minor components like phytoestrogens and vitamin E may have contributed to this effect.</span>
Androgenic alopecia,Cucurbita pepo,pumpkin seed oil
https://ajp.mums.ac.ir/article_13463.html
https://ajp.mums.ac.ir/article_13463_1299d209b85bc3523e47e1ac4a62c2da.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
6
2019
11
01
Evaluation of the effectiveness of crocin isolated from saffron in treatment of burning mouth syndrome: A randomized controlled trial
505
516
EN
Atessa
Pakfetrat
Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
pakfetrat@mums.ac.ir
Mahdi
Talebi
Family Medicine Department, Faculty of Psychiatry, Mashhad University of Medical Sciences, Mashhad, Iran.
talebi@mums.ac.ir
Zohreh
Dalirsani
Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
dalirsani@mums.ac.ir
Sayed Ahmad
Mohajery
0000-0002-7907-9625
Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
mohajeria@mums.ac.ir
Roya
Zamani
0000-0002-1967-924X
Oral & Maxillofacial specialist, Department of Oral Health, Gonabad University of Medical Sciences, Gonabad, Iran.
zmnir67@gmail.com
Ala
Ghazi
0000-0002-0064-1233
Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
alaghazi10@gmail.com
10.22038/ajp.2019.12764
<strong>Objective:</strong> Burning mouth syndrome (BMS) is a debilitating disorder with few limited treatment modalities. Because of the proven association between BMS symptoms, and depression and anxiety, treatment modalities that alleviate the two latter etiologic factors can be clinically effective. Thus, owing to the antidepressant and potential analgesic effects of crocin (as an active constituent of saffron), the present study was performed to compare the effect of crocin and citalopram (as control) on BMS symptoms and depression/anxiety in patients with BMS.<br /> <strong>Materials and Methods:</strong> The present double-blind randomized clinical trial was carried out on BMS patients. Patients were randomly divided into citalopram (n=21) and crocin (n=26) groups and treated for 11 weeks. BMS symptoms (based on Visual Analysis Scale (VAS)), as well as anxiety and depression (based on Hamilton questionnaire) were evaluated at baseline and during the treatment period. Mann-Whitney, Chi-Square test, Independent t-test, Friedman, and Spearman correlation were employed for statistical analysis.<br /> <strong>Results:</strong> Our findings showed a significant effect for crocin on the severity of BMS symptoms, anxiety and depression in BMS patients.<br /> <strong>Conclusion:</strong> Crocin can be considered for treatment of BMS subjects with concurrent anxiety and/or depression.
Crocin,Burning mouth syndrome (BMS),Depression,Anxiety,citalopram
https://ajp.mums.ac.ir/article_12764.html
https://ajp.mums.ac.ir/article_12764_0c11d3060e0157fc98231bf6f9d40217.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
6
2019
11
01
Effect of an herbal capsule on chronic constipation among menopausal women: A randomized controlled clinical trial
517
529
EN
Paria
Eliasvandi
0000-0003-2313-1946
Department of Midwifery, School of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran
eliasvandi.p@gmail.com
Laleh
Khodaie
Department of Traditional Medicine, Faculty of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
khodaiel@gmail.com
Sakineh
Mohammad Alizadeh Charandabi
Department of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran
alizades@tbzmed.ac.ir
Mojgan
Mirghafourvand
0000-0001-8360-4309
Department of Midwifery, Social Determinants of Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
mirghafourvand@gmail.com
10.22038/ajp.2019.13109
<strong>Objective:</strong> Chronic constipation is frequently observed in postmenopausal women. An herbal combination including clover plants, Roman anis or Anisone, green anis or fennel, green raisins, Alhagi maurorum, violets, Terminalia chebula, senna and golqand has been introduced in traditional books as an effective laxative. Thus, the present study aimed to assess the effectiveness of the combined herbal capsule on chronic constipation in postmenopausal women.<br /> <strong>Materials and Methods:</strong> This triple blinded, randomized placebo-controlled trial was conducted on 64 postmenopausal women. Individuals were randomly assigned to combined the herbal capsule and placebo groups. The herbal capsule included cloves (4 units), Anise and Anison (6 units each), violet flowers (12 units), Terminalia Chebula and fresh green raisins (25 units each), senna leaves, Alhagi maurorum, and Golqand (50 units each). Constipation questionnaire and the individual’s assessment of constipation symptoms scale were used for data collection.<br /> <strong>Results:</strong> The mean frequency of bowel movements in the herbal capsule group was significantly higher than the placebo group (mean difference=4.2; 95% confidence interval: 0.3 to 4.5; p<0.001). Straining during defecation, stool amount, incomplete defecation, sensation of obstruction during defecation, and need for manipulation to facilitate removal of stools were significantly reduced in the herbal capsule group compared to the placebo group (p<0.001). The amount and consistency of defecation was also improved in the two study groups, but a significant difference was observed between the groups (p<0.001). The mean score of constipation symptoms in the herbal capsule group was significantly reduced compared to the placebo group (-15.4; -11.5 to -19.29; p<0.001).<br /> <strong>Conclusion</strong>: Consumption of herbal capsules improved chronic constipation in postmenopausal women.
Herbal capsule,Chronic constipation,Menopause
https://ajp.mums.ac.ir/article_13109.html
https://ajp.mums.ac.ir/article_13109_b6ae9f6b4b42ca9a3819679efd7a01dc.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
6
2019
11
01
Chemical composition and antibacterial properties of Peganum harmala L.
530
537
EN
Milad
Iranshahy
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
iranshahiml@mums.ac.ir
Sedigheh
Fazli Bazaz
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
fazlis@mums.ac.ir
Giti
Haririzadeh
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
ghoseghozah@gmail.com
Bibi Zahra
Abootorabi
Medicinal Chemistry Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
iranshahiml@hotmail.com
Ali Mohammad
Mohamadi
Pharmaceutical Control Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
ghosegozah@gmail.co
Zahra
khashyarmanesh
0000000347650855
Medicinal Chemistry Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
khashyarmaneshz@mums.ac.ir
10.22038/ajp.2019.13382
<strong>Objective: </strong>The present study was conducted to investigate antibacterial properties of fruit and flower of <em>Peganum harmala</em>.<br /> <strong>Material and Methods: </strong>Column chromatography, followed by preparative thin layer chromatography (TLC) was used for final purification. The structure of pure alkaloids was determined using spectroscopic methods (<sup>1</sup>H-NMR, <sup>13</sup>C-NMR, UV and MS). Smoke and extract of total alkaloids were investigated for antimicrobial activity against five different microorganisms (standards and hospital isolates). The antibacterial activity was evaluated using disc diffusion assay and minimum inhibitory concentration (MIC) was determined by serial dilution methods.<br /> <strong>Results:</strong> Chemical investigation of the chloroform extract of ripe fruit and flower of <em>P. harmala</em> led to identification of three alkaloids in ripe fruit and two alkaloids in the flower and leaves of this plant. Alkaloids identified in ripe fruit were harmine, peganine (vasicine) and harmaline. Two alkaloids, harmine and peganine, were detected in the flower of <em>P. harmala, </em>while harmaline was only found in the ripe fruit. The total alkaloids of flower were compared with total alkaloids of ripe fruit by TLC method. Fruits and flowers had 3.12 and 3.27% alkaloid contents, respectively.<br /> <strong>Conclusion: </strong>Our results showed that the alkaloids and smoke were specifically more effective on <em>Candida albicans</em> and Gram- positive bacteria (<em>Micrococcus luteus</em> and <em>Staphylococcus aureus</em>), while Gram- negative bacteria, especially <em>Pseudomonas aeruginosa</em>,were less sensitive.
Alkaloid,Antimicrobial activity,Peganum harmala,Harmine,Harmaline,Smoke
https://ajp.mums.ac.ir/article_13382.html
https://ajp.mums.ac.ir/article_13382_5a95427b2e7bd8cd65894f63255782d0.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
6
2019
11
01
Thymol has beneficial effects on the experimental model of ulcerative colitis
538
550
EN
Pouriya
Tahmasebi
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
p.tahmasebi@urmia.ac.ir
Seyyed Meysam
Abtahi froushani
0000-0003-2579-6618
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
meysamabtahi@hotmail.com
Nahideh
Afzale Ahangaran
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
n.ahangaran@gmail.com
10.22038/ajp.2019.13383
<strong>Objective:</strong> Thymol, a natural aromatic monoterpene phenol derived from thymus, possesses anti-inflammatory benefits. Here, we evaluated the potential of thymol therapy in improving an animal model of ulcerative colitis.<br /> <strong>Materials and Methods:</strong> Luminal instillation of acetic acid was used to induce colitis in male Wistar rats. Treatment groups daily received prednisolone (2 mg/kg, orally) or thymol (100 mg/kg, orally) for 10 consecutive days. Then, the rats were euthanized and tissue specimens were collected for evaluation of cyclooxygenase-2 (COX-2) expression by immunohistochemistry. Furthermore, the levels of total protein, nitric oxide, myeloperoxidase, malondialdehyde, IL-1, IL-6, and TNF-α were monitored in colonic homogenates. Eventually, the relative mRNA expression of <em>IκBα </em>and <em>NF-κBp65</em> was investigated using reverse-transcriptase PCR (RT-PCR) in colonic homogenates.<br /> <strong>Results:</strong> Both medications could reduce the mortality rate and the clinical scores of ulcerative colitis. The COX-2 expression was significantly reduced in the colons of thymol-treated animals compared to the prednisolone group. Also, the myeloperoxidase activity, nitric oxide level and malondialdehyde intensity were decreased in the colons of thymol-treated animals to a greater extent compared to the prednisolone group. Moreover, the total protein content of guts showed significant increases in the guts of thymol-treated animals in comparison to the prednisolone group. Nonetheless, thymol significantly reduced the levels of IL-6, and IL-1 compared to prednisolone. Both medications caused a significant decrease in the mRNA level of <em>NF-κBp65</em>, though the mRNA level of <em>IκBα</em> did not show significant changes between the groups.<br /> <strong>Conclusion:</strong> Thymol may be a promising agent to ameliorate ulcerative colitis.
Thymol,Acetic-acid,Ulcerative colitis,Wistar rat
https://ajp.mums.ac.ir/article_13383.html
https://ajp.mums.ac.ir/article_13383_40841d5a95b496dfed87ec2636439839.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
6
2019
11
01
Semen quality, hormone profile and histological changes in male albino rats treated with Corchorus olitorius leaf extract
551
562
EN
Daniel
Orieke
0000-0003-2932-2559
Department of Biochemistry,College of Natural and Applied Sciences,Michael Okpara University of Agriculture,Umudike, Nigeria
danielorieke@gmail.com
Obioma Christopher
Ohaeri
Department of Biochemistry, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
professorohaeri@yahoo.com
Ifeoma
Ijeh
0000-0000-0000-0000
Department of Biochemistry, College of Natural and Applied Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
ijehirene@yahoo.com
Ijioma
Solomon
Nnah
0000-0002-4239-5771
Department of Zoology and Environmental Biology Michael Okpara University of Agriculture Umudike, Abia State.
ijiomasolo@yahoo.co.uk
10.22038/ajp.2019.13426
<strong><span style="font-size: medium;">Objective:</span></strong><span style="font-size: medium;"> In this study, the anti-fertility effect of <em>Corchorus olitorius</em></span><span style="font-size: medium;"> leaf extract (COLE) was evaluated in adult male rats. </span><br /> <strong><span style="font-size: medium;">Materials and Methods:</span></strong><span style="font-size: medium;"> Forty rats assigned to 4 groups of 10 rats each, were orally given COLE for 28 days. Group 1 was the control group but groups 2, 3 and 4 were administered with 250, 500 and 1000 mg/kg body weight, respectively and were considered the test groups. Blood collection from the animals was performed at the end of treatments and blood samples were used for reproductive hormone assays. Also, sperm sample quality was ascertained, and basic organs were evaluated histologically for all groups. </span><br /> <strong><span style="font-size: medium;">Results:</span></strong><span style="font-size: medium;"> A significant fall in relative organ weights for the testes and prostate was observed following high-dose treatment (p<0.05). Sperm sample pH, and individual sperm motility, viability, progressiveness and concentration decreased, while total abnormalities increased following high-dose (1000 mg/kg) treatment (p<0.05). Serum concentration of FSH significantly increased at 500 and 1000 mg/kg dose levels while LH and testosterone concentrations were significantly higher than control at all dose levels (p<0.05) except estrogen which was higher than control at 250 and 500 mg/kg but lower at 1000 mg/kg dose level. </span><span style="font-size: medium;">Control testes showed intact histological architecture with mature spermatid density of 300 cells per tubule and well differentiated Leydig cells, while those animals treated with 250 and 500 mg/kg of the extract, were without significant pathology but had average spermatid densities of 200 and 280 cells per tubule, respectively. Significant azoospermia and spermatid density of only 30 cells per tubule and prostatic degeneration were seen in the group treated with 1000 mg/kg body weight of the extract. </span><br /> <strong><span style="font-size: medium;">Conclusion:</span></strong><span style="font-size: medium;"> Consumption of high amounts of <em>C. olitorius</em></span><span style="font-size: medium;"> may inhibit reproductive functions in males.</span>
Corchorus olitorius,Anti-fertility,Hormonal,Sperm sample,Histology
https://ajp.mums.ac.ir/article_13426.html
https://ajp.mums.ac.ir/article_13426_963a604358faeaa7b1c1bb19a90d94ec.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
6
2019
11
01
Therapeutic potential of Origanum vulgare leaf hydroethanolic extract against renal oxidative stress and nephrotoxicity induced by paraquat in rats
563
573
EN
Ali
Sharifi-Rigi
Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
alisharifi1041@gmail.com
Esfandiar
Heidarian
0000-0002-4701-144X
Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
heidarian46@yahoo.com
10.22038/ajp.2019.13466
<span style="font-size: medium;"><strong>Objective: </strong>Paraquat is a herbicide with potent toxicity in humans and animals. This study aimed to evaluate the protective effects of <em>Origanum vulgare</em></span><span style="font-size: medium;"> (<em>O. vulgare</em></span><span style="font-size: medium;">) leaf extract</span><span style="font-size: medium;"> on the acute nephrotoxicity and renal oxidative stress caused by paraquat.</span><br /> <strong><span style="font-size: medium;">Materials and Methods: </span></strong><span style="font-size: medium;">We randomly assigned forty male rats into five groups (G1-G5). The G1 was used as control; G2 only received paraquat (25 mg/kg body weight (bw)/day, <em>po</em></span><span style="font-size: medium;">); and G3, G4 and G5 received 25 mg/kg b.w/day oral doses of paraquat and </span><em><span style="font-size: medium;">O. vulgare</span></em><span style="font-size: medium;"> hydroethanolic leaf extract (200, 400, 800 mg/kg bw/day, </span><em><span style="font-size: medium;">po</span></em><span style="font-size: medium;">, respectively). After 2 weeks, superoxide dismutase (SOD), renal catalase (CAT), vitamin C levels, histopathological changes, and tumor necrosis factor-α (</span><span style="font-size: medium;"><em>TNF-α</em>) gene expression as well as serum levels of urea, creatinine (Cr), and protein carbonyl (PC) were determined.</span><br /> <span style="font-size: medium;"><strong>Results:</strong> </span><span style="font-size: medium;">In G2, oral administration of paraquat significantly increased (p<0.05) serum Cr, urea, PC, and renal <em>TNF-α</em></span><span style="font-size: medium;"> gene expression</span><span style="font-size: medium;"> relative to those of the control group. Renal catalase, superoxide dismutase, and vitamin C levels were decreased significantly (p<0.05) in G2 as compared to G1. Administration of <em>O. vulgare</em> leaf extract not only increased the renal </span><span style="font-size: medium;">vitamin C, CAT, and SOD but also decreased the renal <em>TNF-α</em></span><span style="font-size: medium;"> gene expression, </span><span style="font-size: medium;">malondialdehyde (MDA), serum urea and creatinine in paraquat-induced nephrotoxicity in rats. </span><br /> <strong><span style="font-size: medium;">Conclusion: </span></strong><span style="font-size: medium;">Our results show that <em>O. vulgare</em></span><span style="font-size: medium;">leaf extract has protective effects against nephrotoxicity induced by paraquat in rats. </span><span style="font-size: medium;">It seems that the nephroprotective effects of <em>O. vulgare </em></span><span style="font-size: medium;">extract may be related to its antioxidant and anti-inflammatory effects.</span>
Antioxidant,Kidney,Nephrotoxicity,Paraquat,Oxidative stress,TNF-α
https://ajp.mums.ac.ir/article_13466.html
https://ajp.mums.ac.ir/article_13466_da0fb9b1b010354619682c0106058151.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
6
2019
11
01
Cytotoxic activity of caffeic acid and gallic acid against MCF-7 human breast cancer cells: An in silico and in vitro study
574
586
EN
Hasan
Rezaei-Seresht
Traditional and Complementary Medicine Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
h.rezaeiseresht@gmail.com
Hamid
Cheshomi
0000-0001-7925-7797
Cellular and Molecular Research Center, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran.
hamid.ch65@gmail.com
Farahnaz
Falanji
Cellular and Molecular Research Center, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
farahnaz_falanji@outlook.com
Fatemeh
Movahedi-Motlagh
Cellular and Molecular Research Center, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran.
movahedi_fatemeh5@yahoo.com
Maryam
Hashemian
Department of Nutrition, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran.
hashemian@yahoo.com
Erfan
Mireskandari
Department of Chemistry, School of Sciences, Hakim Sabzevari University, Sabzevar, Iran
erfanmireskandari@gmail.com
10.22038/ajp.2019.13475
<strong>Objective: </strong>Phenolic compounds have been considered inhibitors of various cancers.<br /> <strong>Material and Methods: </strong>In this study, caffeic acid and gallic acid were appraised for their possible effects on apoptotic genes expression in a breast cancer cell line <em>in vitro</em>. We also evaluated ligand interaction and ligand binding with estrogen receptor alpha by molecular docking. To determine half maximal inhibitory concentration, MCF-7 cells were treated with different concentrations of caffeic acid and gallic acid by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Furthermore, morphological changes in cells and alterations in <em>P53</em>, <em>Mcl-1</em> and <em>P21</em> gene expression were studied by real-time RT-PCR. Also, protein network and different interactions between the desired genes were analyzed using GeneMANIA database.<br /> <strong>Results:</strong> Evaluation of cell survival by MTT assay revealed that the half-maximal inhibitory concentration values for caffeic acid and gallic acid against MCF-7 cells, were 159 and 18 µg/ml, respectively. These compounds were found to affect <em>P53</em>, <em>Mcl-1</em> and <em>P21</em> gene expression; this alteration in gene expression probably occurred along with the activation of intrinsic apoptotic signaling pathway.<br /> <strong>Conclusion: </strong>Via apoptosis induction, caffeic acid and gallic acid have induce toxic effects and morphological changes in breast cancer cells, suggesting their possible future application as antitumor agents.
Caffeic acid,Gallic acid,MCF-7,P53,P21,Mcl-1
https://ajp.mums.ac.ir/article_13475.html
https://ajp.mums.ac.ir/article_13475_a5cdc3ef59ced6717c4f531dfed9f0cb.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
6
2019
11
01
Zataria multiflora Boiss. extract improves spatial memory and learning capacity in scopolamine-induced amnesic rats
587
596
EN
Vahid
Sheibani
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
vsheibani2@yahoo.com
Ali
Mandegary
Pharmaceutical Research Center, Neuroscience Institute, Department of Toxicology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
alimandegary@yahoo.com
Elham
Vazifekhahan
Herbal and Traditional Research Center, Department of Pharmacognosy, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
e.vazifekhahan@yahoo.com
Zahra
Kasbzade
Herbal and Traditional Research Center, Department of Pharmacognosy, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
kasbzade.zahra@gmail.com
Amir
Asadi
Herbal and Traditional Research Center, Department of Pharmacognosy, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
asadi_phyto87@yahoo.com
Fariba
Sharififar
0000-0002-9057-1987
Herbal and Traditional Research Center, Department of Pharmacognosy, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
fa.sharififar@gmail.com
10.22038/ajp.2019.13540
<span style="font-size: medium;"><strong>Objective: </strong><em>Zataria multiflora</em></span><span style="font-size: medium;"> (<em>Zm</em></span><span style="font-size: medium;">) has been proposed for memory enhancing in Persian traditional medicine; but to now, no study has been carried out in this field yet. The aim of this research was to study the plant effect on spatial memory in scopolamine-induced amnesia and investigate </span><em><span style="font-size: medium;">in vivo</span></em><span style="font-size: medium;"> anticholinesterase effect of </span><em><span style="font-size: medium;">Zm</span></em><span style="font-size: medium;">. </span><br /> <strong><span style="font-size: medium;">Material and Methods: </span></strong><span style="font-size: medium;">Aerial parts of the plant were extracted with methanol and standardized on the basis of rutin content. Male rats received three doses of <em>Zm</em></span><span style="font-size: medium;"> extract (100, 200 and 400 mg/kg, intraperitoneal (ip) for 7 days) and 30 min after the latest dose, scopolamine (1 mg/kg) was administered to animals. Learning capacity and spatial memory were studied using morris water maze (MWM) and passive avoidance test (PAT) methods. Anticholinesterase activity was studied using Ellman</span><span style="font-size: medium;">’s method. Physostigmine (0.3 mg/kg) and piracetam (200 mg/kg) were used as positive controls. </span><br /> <span style="font-size: medium;"><strong>Results:</strong> </span><span style="font-size: medium;">All doses of <em>Zm</em></span><span style="font-size: medium;"> extract significantly decreased the distance and time spent to find the platform in MWM and increased the time latency in PAT test. In both MWM and PAT tests, the highest effect of </span><em><span style="font-size: medium;">Zm</span></em><span style="font-size: medium;"> was observed at 200 mg/kg which was in accordance with AChE inhibitory effect of the plant</span><span style="font-size: medium;">. </span><br /> <strong><span style="font-size: medium;">Conclusion: </span></strong><span style="font-size: medium;">Our findings indicate that <em>Zm </em></span><span style="font-size: medium;">has anti-amnesic effect and might improve memory deficit through anticholinesterase activity.</span>
Anticholinesterase,Cognitive disorder,Morris Water Maze,Passive avoidance test,Zataria multiflora
https://ajp.mums.ac.ir/article_13540.html
https://ajp.mums.ac.ir/article_13540_f1d0ecd931b69ae2f2257ca4ffd2bff6.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
6
2019
11
01
Effects of troxerutin on inflammatory cytokines and BDNF levels in male offspring of high-fat diet fed rats
597
605
EN
Maryam
Hoseindoost
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
mhh.hosseindoost@gmail.com
Mohammad Reza
Alipour
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
alipourmr52@yahoo.com
Fereshteh
Farajdokht
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
farajdokht@gmail.com
Roghayeh
Diba
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
r1367diba@gmail.com
Parvin
Bayandor
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
bayandorbarzegari@gmail.com
Keivan
Mehri
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
keivanm20@gmail.com
Sepehr
Nayebi Rad
Student Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.
nayebisepehr76@gmail.com
Shirin
Babri
0000-0002-0256-3152
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
shirinb46@yahoo.com
10.22038/ajp.2019.13587
<span style="font-size: medium;"><strong>Objective:</strong> In the present study, we aimed to examine the effect of troxerutin treatment on levels of brain-derived neurotrophic factor (BDNF), and tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), as pro-inflammatory cytokines, in the blood and hippocampus of high-fat diet (HFD) fed dams and their male offspring. </span><br /> <span style="font-size: medium;"><strong>Materials and Methods:</strong> Forty virgin female Wistar rats, 3 weeks old, were divided into two groups (n=20/per group) and fed control diet (CD), or HFD for 8 weeks. After mating, pregnant animals were assigned to four subgroups including: control (CD), control+troxerutin (CD+T), high-fat diet (HFD), and HFD+troxerutin (HFD+T) groups. HFD was continued during pregnancy and lactation. Troxerutin (150 mg/kg/day, P.O.) was administered during pregnancy in the CD+T and HFD+T groups. On postnatal day (PND) 21, male offspring were separated and fed a normal diet until PND 90. Inflammatory cytokines (TNF-α, and IL-6) and BDNF levels were measured in the serum and hippocampal samples using rat-specific enzyme-linked immunosorbent assay (ELISA) kits. </span><br /> <span style="font-size: medium;"><strong>Results:</strong> Our findings showed a significant increase in the serum levels of TNF-α and IL-6, but a decrease in BDNF levels in the serum of HFD-fed dams in comparison with CD group, which was reversed by troxerutin. Moreover, troxerutin treatment, during pregnancy, significantly decreased TNF-α and IL-6 levels, but increased BDNF level in the serum and hippocampus of HFD+T offspring in comparison with HFD offspring. </span><br /> <span style="font-size: medium;"><strong>Conclusion:</strong> These results showed that troxerutin could prevent the harmful effects of maternal HFD on their offspring through inhibition of pro-inflammatory cytokines and elevation of BDNF levels.</span>
Troxerutin,Maternal high fat diet,BDNF,Inflammatory cytokines
https://ajp.mums.ac.ir/article_13587.html
https://ajp.mums.ac.ir/article_13587_9ec1a44ab85bf825feea0bef943ab67c.pdf