Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
3
2019
05
01
Capsaicin inhibitory effects on Vibrio cholerae toxin genes expression
187
194
EN
Soroor
Erfanimanesh
Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
s_erfanimanesh@yahoo.com
gita
eslami
Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
g_eslami@yahoo.com
Arezou
Taherpour
Department of Microbiology, Kurdistan University of Medical Sciences, Sanandaj, Iran
are_taherpour@yahoo.com
Ali
Hashemi
0000-0002-7258-5541
Depratment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
hashemi1388@yahoo.com
10.22038/ajp.2018.11736
<strong><span style="font-size: medium;">Objective: </span></strong><span style="font-size: medium;">Cholera is an acute secretory diarrhea caused by the Gram-negative bacterium, <em>Vibrio cholerae </em></span><span style="font-size: medium;">mostly through production of cholera toxin (CT) and zonula occludens toxin (Zot)</span><em><span style="font-size: medium;">.</span></em><span style="font-size: medium;"> Isolates of </span><em><span style="font-size: medium;">V. cholerae</span></em><span style="font-size: medium;"> have acquired resistance elements during the last decade. One of the most promising ways to treat resistant strains is to use antivirulence agents instead of killing the causative agent with conventional antibiotics. In this study, we examined whether different concentrations of capsaicin - the pungent fraction of red chili- can act as an antivirulence agent and inhibit </span><em><span style="font-size: medium;">V. cholerae</span></em><span style="font-size: medium;"> toxin production.</span><br /> <strong><span style="font-size: medium;">Materials and Methods:</span></strong><span style="font-size: medium;"> Two standard strains namely, <em>V. cholerae </em></span><span style="font-size: medium;">ATCC 14035 and </span><em><span style="font-size: medium;">V. cholerae</span></em><span style="font-size: medium;"> PTCC 1611 were used in this study.</span><span style="font-size: medium;"> Minimum Inhibitory Concentration (MIC) of capsaicin </span><span style="font-size: medium;">was determined by broth microdilution method. Based on MIC results, the bacteria were cultured in the presence of sub-MIC concentrations of capsaicin and a negative control without capsaicin. Real-time PCR (RT-PCR) was carried out to determine the expression level of </span><em><span style="font-size: medium;">V. cholerae </span></em><span style="font-size: medium;">toxin genes at each concentration.</span><br /> <strong><span style="font-size: medium;">Results:</span></strong><span style="font-size: medium;"> MIC test showed that 200 mg/mL of capsaicin in 2% dimethyl sulfoxide (DMSO) could inhibit the growth of the two standard strains of <em>V. cholera</em></span><span style="font-size: medium;">e. The expression of </span><em><span style="font-size: medium;">V. cholerae </span></em><span style="font-size: medium;">toxin genes was significantly reduced following treatment with sub-MIC concentrations of capsaicin as assessed by RT-PCR.</span><br /> <strong><span style="font-size: medium;">Conclusion:</span></strong><span style="font-size: medium;"> Capsaicin showed great inhibitory effect against cholera toxin and reduced Zot production in the tested strains of <em>V. cholerae</em></span><span style="font-size: medium;">. The results showed promising insights into antivirulence effects of capsaicin.</span>
Vibrio cholera,Antivirulence agents,Capsaicin,Toxin gene expression
https://ajp.mums.ac.ir/article_11736.html
https://ajp.mums.ac.ir/article_11736_79084788f3829da7e04045d60fee065f.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
3
2019
05
01
Clinical and experimental effects of Nigella sativa and its constituents on respiratory and allergic disorders
195
212
EN
Zahra
Gholamnezhad
0000-0001-6280-7246
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
gholamnezhadz@mums.ac.ir
Farzaneh
Shakeri
Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
f_1366_sh@yahoo.com
Saiedeh
Saadat
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
saadats931@mums.ac.ir
Vahideh
Ghorani
0000-0001-6883-7972
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
ghoranisv921@mums.ac.ir
Mohammad Hossein
Boskabady
0000-0001-5736-9755
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
boskabadymh@mums.ac.ir
10.22038/ajp.2019.12196
<strong>Objective: </strong>Black cumin or <em>Nigella sativa</em> (<em>N. sativa</em>) seed has been widely used traditionally as a medicinal natural product because of its therapeutic effects. In this review, the medicinal properties of <em>N. sativa</em> as a healing remedy for the treatment of respiratory and allergic diseases, were evaluated.<br /> <strong>Material and Methods: </strong>Keywords including <em>Nigella sativa</em>, black seed, thymoquinone, respiratory, pulmonary, lung and allergic diseases were searched in medical and nonmedical databases (i.e. PubMed, Science Direct, Scopus, and Google Scholar). Preclinical studies and clinical trials published between 1993 and 2018 were selected.<br /> <strong>Results:</strong> In experimental and clinical studies, antioxidant, immunomodulatory, anti-inflammatory, antihistaminic, antiallergic, antitussive and bronchodilatory properties of <em>N. sativa</em> different extracts, extracts fractions and constituents were demonstrated. Clinical studies also showed bronchodilatory and preventive properties of the plant in asthmatic patients. The extract of <em>N. sativa</em> showed a preventive effect on lung disorders caused by sulfur mustard exposure. The therapeutic effects of the plant and its constituents on various allergic disorders were also demonstrated.<br /> <strong>Conclusion: </strong>Therefore, <em>N. sativa</em> and its constituents may be considered effective remedies for treatment of allergic and obstructive lung diseases as well as other respiratory diseases.
Nigella Sativa,Thymoquinone,Experimental effect,Clinical effect,Respiratory diseases,Allergic diseases
https://ajp.mums.ac.ir/article_12196.html
https://ajp.mums.ac.ir/article_12196_792541697ef14eb490fa54af5aad07a2.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
3
2019
05
01
Protective effect of Berberis vulgaris on Fenton reaction-induced DNA cleavage
213
220
EN
Nooshin Sadat
Asadi
Department of Biology, Faculty of Science, Yazd University, Yazd, Iran
mehrnush_asadi@yahoo.com
Mohammad Mehdi
Heidari
0000-0002-3328-4746
Department of Biology, Faculty of Science, Yazd University, Yazd, Iran
heidarimm@yazd.ac.ir
Mehri
Khatami
Department of Biology, Faculty of Science, Yazd University, Yazd, Iran
m.khatami@yazd.ac.ir
10.22038/ajp.2018.11788
<span style="font-size: medium;"><strong>Objective:</strong><em> Berberis vulgaris</em></span><span style="font-size: medium;"> contains antioxidants that can inhibit DNA cleavage. The purpose of this study was to evaluate the antioxidant and protective activity of <em>B. vulgaris</em></span><span style="font-size: medium;"> on DNA cleavage.</span><br /> <strong><span style="font-size: medium;">Materials and Methods:</span></strong><span style="font-size: medium;"> In this study, the antioxidant capacity of <em>B. vulgaris </em></span><span style="font-size: medium;">was investigated using DPPH and its protective effect was evaluated on </span><em><span style="font-size: medium;">pBR322</span></em><span style="font-size: medium;"> plasmid and lymphocyte genomic DNA cleavage induced by Fenton reaction, by DNA electrophoresis.</span><br /> <strong><span style="font-size: medium;">Results:</span></strong><span style="font-size: medium;"> Aqueous extract of <em>B. vulgaris</em></span><span style="font-size: medium;"> presented dual behavior with a potent antioxidant activity at 0.25and 0.75mg/ml for pBR322 plasmid and lymphocyte genomic DNA, respectively, but a pro-oxidant activity was observed at higher concentrations.</span><br /> <strong><span style="font-size: medium;">Conclusion:</span></strong><span style="font-size: medium;"> Our results indicated that <em>B. vulgaris</em></span><span style="font-size: medium;"> extract an inhibit Fenton reaction-induced DNA cleavage and oxidative cleavage of double-stranded DNA assay is a powerful technique that can be used to determine the antioxidant and pro-oxidant properties of a compound on cellular components such as DNA.</span>
Antioxidant activity,Berberis vulgaris,DNA damage,Fenton reaction
https://ajp.mums.ac.ir/article_11788.html
https://ajp.mums.ac.ir/article_11788_a4f9d93a31be7b2a8495495ea302f212.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
3
2019
05
01
Anti-inflammatory and anti-neuropathic effects of a novel quinic acid derivative from Acanthus syriacus
221
236
EN
Karim
M
Raafat
0000-0001-5907-6956
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Beirut Arab University, 115020 Beirut, Lebanon
k.raafat@bau.edu.lb
10.22038/ajp.2019.12218
<span style="font-size: medium;"><strong>Objective: </strong><em>Acanthus syriacus</em></span><span style="font-size: medium;"> (AS) is one of the valuable herbal plants with immunomodulatory potentials. The aim of this study is to assemble a phytochemical investigation of <em>A. syriacus</em></span><span style="font-size: medium;"> exploring its anti-inflammatory and antinociceptive properties, identification of its most active compound(s) and elucidating their structure and determining their mechanisms of action. </span><br /> <strong><span style="font-size: medium;">Materials and Methods:</span></strong><span style="font-size: medium;"> Bio-guided fractionation and isolation-schemes were used utilizing RP-HPLC, CC, </span><sup><span style="font-size: small;">1</span></sup><span style="font-size: medium;">H- and </span><sup><span style="font-size: small;">13</span></sup><span style="font-size: medium;">C-NMR, and biological-models were used to evaluate their effects against inflammation and neuropathic-pain (NP). </span><br /> <span style="font-size: medium;"><strong>Results:</strong> The outcomes showed that the most active fraction (FKCA) of AS was identified. Two of the three components of FKCA were identified by chromatographic-methods, while the third compound was isolated, its structure was elucidated and its was named Kromeic acid (KRA); FKCA contained Ferulic acid (27.5%), kromeic acid (48.1%), and chlorogenic acid (24.4%). AS, FKCA and KRA showed significant (p˂0.05) anti-inflammatory and antinociceptive potentials in the management of allodynia and thermal-hyperalgesia in NP. AS and FCKA showed comparatively equipotent antinociceptive-effects. FKCA showed higher antinociceptive effects than KRA suggesting additive-effects among FKCA components. The anti-inflammatory, insulin secretagogue, oxidative-stress reducing, and protective effects against NO-induced neuronal-toxicity might be amongst the possible mechanisms of tested compounds to alleviate NP. </span><br /> <strong><span style="font-size: medium;">Conclusion:</span></strong><span style="font-size: medium;"> Here, we report the isolation and structure elucidation of a novel quinic-acid derivative, KRA. <em>A. syriacus,</em></span><span style="font-size: medium;"> FKCA, and KRA might be used as a novel complementary approach to ameliorate a variety of painful-syndromes.</span>
Novel quinic acid derivative,Acanthus syriacus,Anti-inflammatory,antinociceptive effects,Kromeic acid
https://ajp.mums.ac.ir/article_12218.html
https://ajp.mums.ac.ir/article_12218_e7dc083115e0386fcae385ccf716bad7.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
3
2019
05
01
Interactive anticancer effect of nanomicellar curcumin and galbanic acid combination therapy with some common chemotherapeutics in colon carcinoma cells
237
247
EN
Arash
Jafari
School of Medicine, Birjand University of Medical Sciences, Birjand, Iran
arashmd@gmail.com
Manouchehr
Teymouri
0000-0002-7855-4519
Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.
manochehr.teimory@gmail.com
Maryam
Ebrahimi Nik
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
ebrahiminm921@mums.ac.ir
Azam
Abbasi
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
abbasia2@mums.ac.ir
Mehrdad
Iranshahi
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
iranshahim@mums.ac.ir
Mohammad Yahya
Hanafi-Bojd
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
my-hanafi_bojd@yahoo.com
Mahmoud Reza
Jafari
0000-0003-3908-6828
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
jafarimr@mums.ac.ir
10.22038/ajp.2019.12265
<span style="font-size: medium;"><strong>Objective:</strong> In the current investigation, we aimed to study the combined cytotoxicity of curcumin, as a nanomicellar formulation, and galbanic acid (Gal), dissolved in DMSO against the murine C26 and human Caco-2 colon carcinoma cells. Further, curcumin potential for cisplatin and doxorubicin (Dox) co-therapy was studied. </span><br /> <span style="font-size: medium;"><strong>Materials and Methods:</strong> The combined cytotoxic effect of these phytochemicals at varying dose ratios were examined using the MTT colorimetric assay. Moreover, the time-dependent toxicity of curcumin, cisplatin, Dox, and pegylated liposomal Dox (Doxil) was determined. The interactive anti-proliferative behavior of these compounds was examined using the CompuSyn software. </span><br /> <span style="font-size: medium;"><strong>Results:</strong> Nanomicellar curcumin showed considerable cytotoxicity in C26 cells 24 hr post-treatment. Co-treatment of cells with curcumin nanomicelles: Gal had a synergistic effect in C26 (at 10:1 molar ratio), and Caco-2 (at 1:5 molar ratio) cell lines in cell cultures. Nanomicellar curcumin showed strong and mild synergistic inhibitory effects in C26 cells when co-administered with Doxil and cisplatin, respectively. </span><br /> <span style="font-size: medium;"><strong>Conclusion:</strong> Curcumin nanomicelles and Gal had a synergistic effect in C26 and Caco-2 cell lines. It is speculated that nanomicellar curcumin shows synergistic cancer cell killing if administered 24-hr post-injection of Doxil and cisplatin. </span>
Nanomicellar curcumin,Galbanic acid,Doxil,Cisplatin,Combination therapy,Synergism
https://ajp.mums.ac.ir/article_12265.html
https://ajp.mums.ac.ir/article_12265_6faf6229bbbccc14d39a04a7403c7a24.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
3
2019
05
01
Evaluation of the protective and therapeutic effects of Pistacia atlantica gum aqueous extract on cellular and pathological aspects of experimental asthma in Balb/c mice
248
259
EN
Zaynab
Shakrami
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
shakarami_60@yahoo.com
hadi
Esmaeili Gouvarchin ghaleh
Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
h.smaili1369@gmail.com
bahman
mansouri motlagh
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
b.mansori68@gmail.com
Ali
Sheikhian
0000-0002-7048-7710
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences.
alisheikhian@gmail.com
Bahman
Jalali Kondori
0000-0002-6134-9965
Faculty of medicine, Baqiyatallah University of medical sciences, Tehran, Iran
bahmanjalali2010@gmail.com
10.22038/ajp.2019.12310
<span style="font-size: medium;"><strong>Objective:</strong> </span><span style="font-size: medium;">The purpose of this study was to investigate the protective and therapeutic effects of aqueous extract of <em>P. atlantica </em></span><span style="font-size: medium;">gum on an experimental asthma in BALB/c mice.</span><br /> <span style="font-size: medium;"><strong>Materials and Methods:</strong> Aqueous extract </span><span style="font-size: medium;">of dried and milled <em>P. atlantica </em></span><span style="font-size: medium;">gum was assemble and</span><span style="font-size: medium;">evaluate by GC-MS. </span><span style="font-size: medium;">In order to investigate the effect of </span><em><span style="font-size: medium;">P. atlantica </span></em><span style="font-size: medium;">gum extract on cellular and pathological aspects of asthma, 60 BALB/c mice were divided into six groups as: negative control, asthmatic group, asthmatic group receiving dexamethasone (1mg/kg; intraperitoneal (IP)) and three asthmatic groups receiving different concentrations of the extract (100, 200 and 400 mg/kg, orally) from the beginning of the study and continued for 84 days. The examined parameters included cell population, IgE antibody production, levels of IL-4, IL-5,</span><span style="font-size: medium;"> TGF-β, INF-γ, IL-10, and IL-17 cytokines, and lung tissue damage. </span><br /> <strong><span style="font-size: medium;">Results:</span></strong><span style="font-size: medium;"> Regardless of the dose, aqueous extract of <em>P. atlantica </em></span><span style="font-size: medium;">gum, caused significant decrease in the number of BALF eosinophilic cells and levels of anti-ovalbumin IgE, IL-4, IL-5 and IL-17 cytokine levels, as well as pathologic damage of the lung tissue. In addition, the amount of anti-inflammatory IL-10, TGF-β, and INF-γ Th1 cytokines significantly increased in the extract-treated groups compared to the asthmatic and dexamethasone-treated groups. Moreover, IFN-γ/IL-4 ratio significantly increased in a dose-dependent manner compared to the un-treated asthma group. </span><br /> <strong><span style="font-size: medium;">Conclusion:</span></strong><span style="font-size: medium;"> The aqueous extract of <em>P. atlantica </em></span><span style="font-size: medium;">gum can be considered as a potent anti-inflammatory and immunomodulatory compound and </span>may be used as a natural compound for treatment of immune system disorders.
Asthma,P. atlantica,Balb/C mouse
https://ajp.mums.ac.ir/article_12310.html
https://ajp.mums.ac.ir/article_12310_e895ddbc373ff8ca357b7be938a891bc.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
3
2019
05
01
Effect of ointment-based egg white on healing of second- degree wound in burn patients: a triple-blind randomized clinical trial study
260
270
EN
Simin
Jahani
Nursing Care Research Center in Chronic Diseases, Nursing and Midwifery School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
ashrafizadeh.h@ajums.ac.ir
Hadis
Ashrafizadeh
0000-0002-9369-5854
Nursing and Midwifery School, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
ashrafizadeh.h1993@gmail.com
Kamran
Babai
Department of Plastic Surgery, Taleghani Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
babaikamran@yahoo.com
Amir
Siahpoosh
Medical Plants Research Center and Department of Pharmacology, School of Pharmacy, Jundishapur University of Medical Sciences, Ahvaz, Iran.
amirsiahpoosh@yahoo.com
Bahman
Cheraghian
Department of Biostatistics and Epidemiology, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
cheraghian2000@yahoo.com
10.22038/ajp.2019.12597
<strong>Objective:</strong> Burn wound healing is one of the problems of medical sciences and it is of great importance to find a drug or substance that can heal burn wounds with minimum complications. The present study aimed to evaluate the effect of ointment-based egg white on healing second-degree burn wounds.<br /> <strong>Materials and Methods:</strong> In the present triple-blind clinical trial, a total of 90 patients from Taleghani hospital, Ahvaz, Iran were selected and randomly divided into two groups based on the inclusion criteria. The intervention group was dressed with egg white formulation + silver sulfadiazine cream and the control group was treated with placebo + silver sulfadiazine cream. The burn wound healing process was evaluated on days 1, 7 and 15 by the Bates-Jensen wound assessment tool.<br /> <strong>Results:</strong> The mean scores of wound healing were decreased (13.75±1.83) in the intervention group when compared to the control (21.51±5.7) on day 15 (p<0.001). The mean duration of wound healing, wound depth, edges, undermining, necrotic tissue, amount of necrosis, exudate type and amount, surrounding skin color, wound induration, peripheral edema, granulation, and epithelialization were significantly decrease in intervention group in comparison with control (p<0.001).<br /> <strong>Conclusion:</strong> The findings of this research showed that egg whites formulation is an appropriate treatment for burn wound healing, reduced above-noted burn wounds’ variables. It seems that this treatment, along with the common medicine, improves chronic wound recovery rate and patients’ health status.
Burn,Egg white,Traditional Medicine,silver sulfadiazine,patient
https://ajp.mums.ac.ir/article_12597.html
https://ajp.mums.ac.ir/article_12597_892da919dcec72ef36767c9c5f87bc0f.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
3
2019
05
01
The neuroprotective mechanism of cinnamaldehyde against amyloid-β in neuronal SHSY5Y cell line: The role of N-methyl-D-aspartate, ryanodine, and adenosine receptors and glycogen synthase kinase-3β
271
280
EN
Masoumeh
Emamghoreishi
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
emam@sums.ac.ir
Majid
Reza
Farrokhi
Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
farokhim@sums.ac.ir
Atena
Amiri
Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
atenaamiri5@gmail.com
Mojtaba
Keshavarz
0000-0003-2863-1309
Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
moj.ph60@yahoo.com
10.22038/ajp.2019.12385
<span style="font-size: medium;"><strong>Objective:</strong> Cinnamaldehyde may be responsible for some health benefits of cinnamon such as its neuroprotective effects. We aimed to investigate the cinnamaldehyde neuroprotective effects against amyloid beta (Aβ) in neuronal SHSY5Y cells and evaluate the contribution of N-methyl-D-aspartate (NMDA), ryanodine, and adenosine receptors and glycogen synthase kinase (GSK)-3β, to its neuroprotective effects. </span><br /> <span style="font-size: medium;"><strong>Materials and Methods:</strong> After seeding the cells in 96-well plates, adenosine (20, 40, 80, and 120 µM), NMDA (20, 40, 80, and 120 µM), and dantrolene (as a ryanodine receptor antagonist; 2, 4, 6, 8, and 16 µM) were added to the medium containing Aβ25-35 and/or cinnamaldehyde. The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide method was used to assess neurotoxicity and western blot to measure the GSK-3β protein level. </span><br /> <span style="font-size: medium;"><strong>Results:</strong> Cinnamaldehyde (15, 20, 23, and 25 μM) significantly reversed Aβ-induced toxicity in SHSY5Y neuronal cells. Adenosine (20, 40, 80 and 120 μM) inhibited the neuroprotective effects of cinnamaldehyde (15 μM). NMDA (20, 40, 80, and 120 μM) reduced cinnamaldehyde (15 and 23 μM) neuroprotective effects against Aβ neurotoxicity. Dantrolene (2, 4, 8, and 16 μM) significantly reduced cinnamaldehyde (15 μM) neuroprotective effects. Cinnamaldehyde (15 and 23 μM) suppressed the Aβ-induced increment of GSK-3β protein level. </span><br /> <strong>Conclusion:</strong> NMDA and adenosine receptors suppression together with ryanodine receptors stimulation may be relevant to cinnamaldehyde neuroprotective effects against Aβ neurotoxicity. Moreover, the inhibition of GSK-3β may contribute to the cinnamaldehyde neuroprotection.
Adenosine,Cinnamaldehyde,Dantrolene,Glycogen Synthase Kinase,Neuroprotection,N-methyl-D-aspartate
https://ajp.mums.ac.ir/article_12385.html
https://ajp.mums.ac.ir/article_12385_0f28a528aa92e306655a178fb4804ea5.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
9
3
2019
05
01
Effects of Boswellia serrata resin extract on motor dysfunction and brain oxidative stress in an experimental model of Parkinson’s disease
281
290
EN
Parvaneh
Doaee
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
pdoaee@yahoo.com
Ziba
Rajaei
0000-0002-5821-5464
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
rajaeiz@med.mui.ac.ir
Mehrdad
Roghani
Department of Physiology, School of Medicine, Shahed University of Medical Sciences, Tehran, Iran.
mehjour@yahoo.com
Hojjatallah
Alaei
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I. R. Iran
alaei@med.mui.ac.ir
Mohammad
Kamalinejad
0009-0006-6373-1320
Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
mkamalinejad@yahoo.com
10.22038/ajp.2019.12403
<strong><span><span style="font-size: medium;">Objective:</span></span></strong><span><em><span style="font-size: medium;">Boswellia serrata </span></em></span><span lang="EN-CA"><span style="font-size: medium;">oleo-gum resin </span></span><span><span style="font-size: medium;">(frankincense) exerted antioxidant and anti-inflammatory effects against several diseases, such as; asthma, rheumatoid arthritis and irritable bowel syndrome. In the current study, the influences of </span><em><span style="font-size: medium;">B. serrata </span></em><span style="font-size: medium;">resin extract</span><span style="font-size: medium;">on motor dysfunction and oxidative stress markers were investigated in the intrastriatal </span></span><span lang="EN-CA"><span style="font-size: medium;">6-hydroxydopamine</span></span><span><span style="font-size: medium;"> (6-OHDA) model of Parkinson’s disease (PD). </span></span><br /> <strong><span><span style="font-size: medium;">Materials and Methods:</span></span></strong><span><span style="font-size: medium;">The animals were randomly assigned to sham, lesion (6-OHDA), and three lesion groups treated with e</span></span><span><span style="font-size: medium;">thyl alcoholic extract of </span><em><span><span style="font-size: medium;">B. serrata </span></span></em><span><span style="font-size: medium;">at doses of 125, 250 and 500 mg/kg for 3 weeks. </span></span></span><span><span style="font-size: medium;">The neurotoxin 6-OHDA (12.5 µg) was microinjected into the left striatum to induce PD in male rats. Motor behavior was assessed by rotational and elevated narrow beam tests. Oxidative stress markers were measured in striatal and midbrain homogenates. </span></span><br /> <strong><span><span style="font-size: medium;">Results:</span></span></strong><span><span style="font-size: medium;"> There was a significant increase in contralateral rotations in 6-OHDA group versus sham group (p<0.001), and treatment with </span><em><span style="font-size: medium;">B. serrata </span></em><span style="font-size: medium;">resin extract at doses of 125 and 250 mg/kg significantly decreased the rotations in comparison to 6-OHDA group (p<em><span style="font-size: medium;">B. serrata</span></em><span style="font-size: medium;"> extract at doses of 125, 250 and 500 mg/kg caused a significant reduction in the latency and total time (p<0.001, p<0.001, and p<0.01, respectively). Biochemical analysis showed no significant difference in oxidative stress markers levels among the groups.</span></span><br /> <strong><span><span style="font-size: medium;">Conclusion:</span></span></strong><span><span style="font-size: medium;"> Our findings suggest that </span><em><span style="font-size: medium;">B. serrata</span></em><span style="font-size: medium;"> resin extract acts as an anti-inflammatory and antioxidant agent that protects nigrostriatal dopaminergic neurons and improve motor impairments in PD.</span></span></span>
Boswellia serrata,Motor dysfunction,Oxidative stress,Parkinson’s disease
https://ajp.mums.ac.ir/article_12403.html
https://ajp.mums.ac.ir/article_12403_e3fce5480edc9f7d4c72ff5e1bfde3a9.pdf