Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
Capparis spinosa reduces Doxorubicin-induced cardio-toxicity in cardiomyoblast cells
489
494
EN
Seyed Hadi
Mousavi
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
mousavish@mums.ac.ir
Azar
Hosseini
0000-0002-3423-0539
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
hoseiniaz@mums.ac.ir
Elham
Bakhtiari
Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
bakhtiarie901@mums.ac.ir
Hassan
Rakhshandeh
0000-0002-2118-1096
Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
rakhshandehh@mums.ac.ir
10.22038/ajp.2016.6207
Objective: Doxorubicin (DOX) is an effective anticancer drug but its clinical application is limited because it induces apoptosis in cardiomyocytes and leads to permanent degenerative cardiomyopathy and heart failure possibly due to oxidative stress. Recent studies showed that <em>Capparis spinosa </em>(<em>C. spinose</em>)exhibits potent antioxidant activity. So, in this study, we explored the protective effect of hydro-alcoholic extract of <em>C. spinosa </em>against DOX-induced cytotoxicity in H9c2 cells. <br/>Materials and Methods: Cell viability was quantified by MTT assay. Apoptotic cells were determined using flow cytometry (sub-G1 peak) evaluation of DNA fragmentation following PI staining. Cells were cultured with 5 μM DOX for 24 hr to induce cell damage. H9c2 cells were pretreated with different concentrations (6-200 μg/ml) of <em>C. spinosa </em>extract for 4 hr before DOX treatment in all trials. <br/>Results: Pretreatment with 25, 50, 100 and 200 µg/ml of <em>C. spinosa</em> could increase the viability of H9C2 cells to 72.63 ± 2.8% (p< 0.05), 77.37 ± 1.8% (p< 0.05), 83.56 ± 2.6% (p< 0.001) and 90.9 ± 0.5% (p< 0.001) of control, respectively. Also, <em>C. spinosa</em> decreased apoptotic induction significantly, at the doses of 50 µg/ml (p<0.05), 100 µg/ml (p<0.01) and 200 µg/ml (p<0.001) <br/>Conclusion: Our results showed that <em>C. spinosa </em>could exert cardioprotective effects against DOX-induced toxicity that might be mediated via its antioxidant activity.
Capparis spinosa,H9C2 cells,Doxorubicin,Apoptosis
https://ajp.mums.ac.ir/article_6207.html
https://ajp.mums.ac.ir/article_6207_5919754ede89b380c54dcd95dff9ab9f.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
Assessment of antibacterial effect of garlic in patients infected with Helicobacter pylori using urease breath test
495
501
EN
Mahmoud
Zardast
0000-0002-7511-2512
Birjand Atherosclerosis And Coronary Artery Research Center, Department of pathology, Birjand University of Medical Sciences (BUMS), Birjand, Iran
dr.zardast@yahoo.com
Kokab
Namakin
Department of pediatrics, Birjand University of Medical Sciences (BUMS), Birjand, Iran
d_namakin@yahoo.com
Jamil
Esmaili kaho
Birjand University of Medical Sciences, Birjand, Iran (BUMS)
esmaili k.@yahoo.com
Sarira sadat
Hashemi
Birjand University of Medical Sciences, Birjand, Iran (BUMS)
sarira.hashemi@yahoo.com
10.22038/ajp.2016.6317
<br/><strong>Objective: </strong><em>Helicobacter pylori </em>(<em>H. pylori</em>) is the most common pathogenic bacteria in the stomach. The aim of the current study was to explore the effect of oral garlic administration on bacterial urease activity inside the stomach and its contribution to the treatment of <em>H. pylori</em> infection. <br/><strong>Materials and Methods</strong>: In this clinical trial, 15 patients were studied quantitatively with Urease Breath Test (UBT). The patients with gastrointestinal symptoms and a positive serum <em>H. pylori</em> IgG were enrolled. UBT was performed for each patient in three sessions as follows: at the beginning of the study, an initial UBT was performed based on which, the positive cases entered the study and the negative ones were excluded. Second UBT was done three days later in patients who were not receiving any treatment and were considered as the control, whereas the third UBT was performed three days after prescribing two medium-sized cloves of garlic (3 g) with their meal, twice a day (at noon and in the evening). The collected data were analyzed using ANOVA and Bonferroni tests and the significance level was set at p<br/><strong>Results</strong>: the mean UBT significantly differed before and after treatment with garlic cloves, being significantly lower after garlic consumption. No meaningful difference was observed in the mean UBT without garlic consumption between the first and second steps. <br/><strong>Conclusion</strong>: Raw garlic has anti-bacterial effects against <em>H. pylori</em> residing in the stomach and may be prescribed along with routine drugs for the treatment of gastric <em>H. pylori</em> infection.
Helicobacter pylori,Urease Breath Test,Garlic,Stomach
https://ajp.mums.ac.ir/article_6317.html
https://ajp.mums.ac.ir/article_6317_3269a98d5464ed8a2f4be44961702360.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
Urological recommedations of Hadji Pasha’s, a Turkish aged doctor in Anatolia
502
505
EN
Mehmet
Yildirim
Department of Urology, Turgut Özal University School of Medicine, Ankara, Turkey
doctorerol@yahoo.com
Metin
Canbal
Department of Family Medicine, Turgut Özal University School of Medicine, Ankara, Turkey
canbalmetin@hotmail.com
Ekrem
Ozyuvali
Department of Urology, Turgut Özal University School of Medicine, Ankara, Turkey
eozyuvali@gmail.com
Omer
Karatas
Department of Urology, Atatürk Research and Training Hospital, Ankara, Turkey
dr_omerfaruk@gmail.com
10.22038/ajp.2016.6499
<br/><strong>Objective: </strong>Urinary tract conditions have been an important part of diseases from antiquity until today. Historically, many plants and herbs have been used for the treatment of urinary disorders. <br/><strong>Methods:</strong> Celâlüddîn Hızır bin Ali el-Konevi (Hadji Pasha) is one of the most famous physician who lived in Anatolia between 13th and 14th centuries. He has written one of the most important medical books of that era, "Müntehab-ıŞifa" (solution of wellness) in Turkish. General medical information about the diseases in this book, focus on diagnosis and treatment. <br/><strong>Results: </strong>The herbal solutions for urological disorders such as, urinary incontinence, urinary stones or erection problems are told in this section.<strong> </strong><br/><strong>Conclusion: </strong>Many of the herbal medicines addressed in this book are being widely used in current medicine, but the usage of these herbals in daily urology practice is limited. In this study, we aimed to share the advices for the urological diseases and therelated herbal medicines that are named in Hadji Pasha’s book, " Müntehab-ıŞifa ", with today's physicians.
Hadji Pasha,Urology,Andrology,Phytotheraphy
https://ajp.mums.ac.ir/article_6499.html
https://ajp.mums.ac.ir/article_6499_e30ba7510f51dc2a85f2fb950f699c02.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
A review study on the effect of Iranian herbal medicines against in vitro replication of herpes simplex virus
506
515
EN
Mohammad-Taghi
Moradi
0000-0002-3103-3740
Students Research Committee, Shahrekord University of Medical Science, Shahrekord, Iran
mtmoradi65@gmail.com
Mamoud
Rafieian-Kopaei
0000-0003-3190-7863
Medical Plants Research Center, Shahrekord University of Medical Science, Shahrekord, Iran
rafieian@yahoo.com
Ali
Karimi
Medical Plants Research Center, Shahrekord University of Medical Science, Shahrekord, Iran
rakarimi1342@gmail.com
10.22038/ajp.2016.6567
<br/><strong>Objective: </strong>There are a number of published data indicating <em>in vitro</em> anti-HSV activity of some of Iranian herbal extracts with no systematic review to discuss these results. Therefore, this article was aimed to review and discuss the methods carried out and the phytochemistry and bioactivity of the extracts used and also conclusions provided in these publications. <br/><strong>Materials and Methods:</strong> Published articles both in English (from Medline, Science Direct, EMBASE, Scopus, Pro Quest, Google scholar, Cochrane Library) and in Persian (from SID, Iran Medex and Magiran) databases, from 1966 to October 2014 were incorporated in this review. The <em>in vitro</em> studies that lacked CC<sub>50</sub>, IC<sub>50</sub>, were excluded. <br/><strong>Results:</strong> Only 42 published reports were found to examine Iranian herbs against HSV replication <em>in vitro. </em>Seventeen out of 42 studies in which 23 kinds of medicinal plants were subjected to crude extraction were included. The review of data showed that some of the herbal extracts including <em>Hyssopus officinalis </em>methanolic extract, <em>Melissa officinalis </em>aqueous extract, <em>Quercus persica </em>L. hydroalcoholic extract and <em>Securigeras ecuridaca </em>methanolic extract with selective index (SI) of 234, 877, >778 and 250, respectively were highly effective against HSV <em>in vitro.</em> <br/><strong>Conclusion:</strong> More comprehensive studies using more advanced methods are needed to be done to achieve promising anti-HSV agents from the bioactive compounds isolated from these herbs.
Herpes Simplex Virus,Herbal Medicine,Iran,In-vitro
https://ajp.mums.ac.ir/article_6567.html
https://ajp.mums.ac.ir/article_6567_591443e221710155defae8e6dac914a7.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
Anti-oxidant and anti-hyperlipidemic activity of Hemidesmus indicus in rats fed with high-fat diet
516
525
EN
Suganya
Venkateshan
Department of Pharmacology, Madras Medical College, Tamil Nadu, India
suganm57@gmail.com
Vetriselvan
Subramaniyan
00-0002-9629-9494
Department of Medicine, Pharmacology Unit, College of Medicine and Health Sciences, Arba Minch University, Ethiopia
vetricology@gmail.com
Velmurugan
Chinnasamy
3epartment of Pharmacology, Sri Krishna Chaithanya College of Pharmacy, Madanapalee, Andhra Pradesh, India
velu0906@gmail.com
Sarath
Chandiran
Department of Pharmaceutical Sciences, Ratnam Institute of Pharmacy, Nellore, India
bahoursarath@gmail.com
10.22038/ajp.2016.6466
<br/><strong>Objective: </strong>Dietary changes playmajor risk roles in oxidative stress andcardiovascular disease and modulate normal metabolic function. The present study was designed to investigate the ameliorative potential of different extracts of <em>Hemidesmus indicus</em> to experimental high-fat diet in wistar rats, and their possible mechanism of action. <br/><strong>Materials and Methods</strong>: Male wistar rats were divided into 6 groups (n=6/group) andfed with a standard diet (control), high-fat diet (HFD), high-fat diet supplemented with different extracts and positive control for 9 weeks. High-fat diet induced changes in average body weight andoxidative stress and elevated levels of plasma lipid profilein rats. <br/><strong>Results</strong>: Oral administration of methanolic extract of <em>H. indicus</em>(200 mg/kg) offered a significant dose-dependent protection against HFD-induced oxidative stress, as reflected in the levels of catalase (p<br/><strong>Conclusion</strong>: The present study revealed that the methanolic extract of <em>H.indicus </em>protects against oxidative stress, hyperlipidemia and liver damage.
Hemidesmus indicus,High fat diet,Oxidative stress,Plasma lipid profile,Antihyperlipidemia,Liver protection
https://ajp.mums.ac.ir/article_6466.html
https://ajp.mums.ac.ir/article_6466_a44fbe0cb2e73ee4ae7108c95629aeea.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
Estragole and methyl-eugenol-free extract of Artemisia dracunculus possesses immunomodulatory effects
526
534
EN
Seyyed Meysam
Abtahi Froushani
0000-0003-2579-6618
Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran
meysamabtahi@hotmail.com
Leila
Zarei
Solid Tumor Research Center, rmia University of Medical Sciences, Urmia, Iran
alireza.alirezai@gmail.com
Hadi
Esmaeili Gouvarchin Ghaleh
0000-0001-8562-2295
Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran
h.smaili69@yahoo.com
Bahman
Mansori Motlagh
Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran
ahmadreza.rez4@gmail.com
10.22038/ajp.2016.6479
<br/><strong>Objective: </strong>Some evidence suggests that chronic uptake of estragole and methyl-eugenol, found in the essential oil of <em>Artemisia dracunculus</em> (tarragon), may be associated with an increased risk of hepato-carcinogenicity. The present study was conducted to investigate the immumodulatory and anti-inflammatory potentials of estragole and methyl-eugenol free extract of tarragon. <br/><strong>Materials and Methods: </strong>Aqueous, hydroalcoholic, methanol and hexane extracts of dried and milled tarragon was prepared and analyzed by GC-MS. The estragole and methyl-eugenol free extract was characterized and used for evaluation of immunity in NMRI mice after challenging with sheep red blood cells. <br/><strong>Results: </strong>It was shown that the aqueous extract of tarragon was free from potentially harmful estragole or methyl-eugenol. Moreover, the immunomodulatory effect of the aqueous extract of tarragon (100 mg/kg for 21 consecutive days) was investigated. The extract significantly increased the level of anti-sheep red blood cells (SRBC (antibody and simultaneously decreased the level of cellular immunity in the treatment group. Moreover, tarragon caused a significant reduction in the production of pro-inflammatory IL-17 and IFN-γ in parallel with a reduction in the ratio of INF-γ to Il-10 or IL-17 to IL-10 in the splenocytes. In addition, the levels of the respiratory burst and nitric oxide production in peritoneal macrophages were significantly decreased. Additionally, the phagocytosis potential of macrophages was significantly increased in treated mice. <br/><strong>Conclusion: </strong>These data showed that the aqueous extract of tarragon may be used as a natural source to modulate the immune system, because it can inhibit pro-inflammatory cytokines and induce anti-inflammatory macrophages.
Artemisia dracunculus (tarragon),Humoral immunity,Cellular immunity,Macrophage
https://ajp.mums.ac.ir/article_6479.html
https://ajp.mums.ac.ir/article_6479_008492b1187255d2ba94c8ed05b44ca6.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
Effect of virgin olive oil versus piroxicam phonophoresis on exercise-induced anterior knee pain
535
541
EN
Babak
Nakhostin-Roohi
Department of Exercise Physiology, Islamic Azad University-Ardabil Branch, Ardabil, Iran
bnakhostin@iauardabil.ac.ir
Faegheh
Khoshkhahesh
Department of Exercise Physiology, University of Mohaghegh- Ardabili, Ardabil, Iran
faegeh_kh@yahoo.com
Shahab
Bohlooli
Department of Pharmacology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran
shahab.bohlooli@arums.ac.ir
10.22038/ajp.2016.6612
<br/><strong>Objective: </strong>The main purpose of this study was to evaluate the effects of virgin olive oil phonophoresis on female athletes' anterior knee pain (AKP). <br/><strong>Materials and Methods:</strong> A double blinded randomized clinical trial was conducted. Ninety-three female athletes suffering from AKP voluntarily participated in this study. Patients were randomly assigned into olive oil (n=31), piroxicam (n=31) or base gel phonophoresis (n=31) groups. At the baseline visit, the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) questionnaire was filled by subjects who were then treated with olive oil, piroxicam or pure phonophoresis for 12 sessions. After 6 and 12 sessions of physiotherapy, subjects filled the questionnaire again. Main outcomes were significant improvement in pain, stiffness, physical function, and total WOMAC scores. <br/><strong>Results:</strong> Although, there was a significant reduction in symptoms of AKP at the end of the therapy in all groups (p< 0.05), but in olive oil group, this improvement was seen after 6 sessions of treatment (p< 0.001). A significant difference between olive oil group and piroxicam and/or phonophoresis group was observed after 6 sessions of therapy (p< 0.05). <br/><strong>Conclusion:</strong> It could be proposed that phonophoresis with virgin olive oil is as effective as piroxicam gel on lowering WOMAC scores of AKP in female athletes and also has several beneficial properties including faster effect and shorter duration of therapy. The exact mechanism of beneficial action of virgin olive oil on AKP is not clear and requires further studies.
Phonophoresis,Olive oil,Piroxicam,Anterior Knee Pain,Topical application
https://ajp.mums.ac.ir/article_6612.html
https://ajp.mums.ac.ir/article_6612_7917fc0aaa345b755b94b8218fcf8bd9.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
Teratogenic effects of silymarin on mouse fetuses
542
549
EN
Mahbobe
Gholami
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Seyed Adel
Moallem
Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad
Afshar
Department of Anatomy, Birjand University of Medical Sciences, Birjand, Iran
Sakineh
Amoueian
Department of Pathology, Imam-Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
Leila
Etemad
Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
etemadl@mums.ac.ir
Gholamreza
Karimi
0000000212735448
Pharmaceutical Research Center, Pharmacy school, Mashhad University of Medical Sciences, Mashhad, Iran
karimig@mums.ac.ir
10.22038/ajp.2016.6679
<br/><strong>Objective: </strong><em>Silybum marianum</em> has been used for centuries in herbal medicine for treatment of liver diseases. Currently, there is no data available on the possible effects of silymarin on fetal development. This study aimed to investigate the teratogenic effect of silymarin on BALB/c mice fetuses. <br/><strong>Materials and Methods</strong>: A total of 40 pregnant mice were divided into 4 groups of 10 mice each. Three groups received silymarin at three different doses of 50, 100 and 200 mg/kg/day during gestational days (GDs). The control group received normal saline and tween (solvent). Dams were sacrificed on GD 18 and all fetuses were examined for gross malformations, size and body weight. Malformed fetuses were double stained with alizarin red and alcian blue. <br/><strong>Results</strong>: Silymarin administration at all doses resulted in reduction of the mean fetal body weights. The abnormalities included limb, vertebral column and craniofacial malformations. Craniofacial malformations were the most common abnormalities, but they were not observed in a dose-dependent manner. The percentage of fetal resorption significantly increased (up to 15%) in all treatment groups. <br/><strong>Conclusion</strong>: Based on our results, silymarin, especially at high doses can lead to fetal resorption, intrauterine growth retardation and limb, vertebral column and craniofacial abnormalities. More precise studies should be conducted about the teratogenic effects of herbal medicine investigating the underlying mechanisms. Thus, caution should be taken when administering <em>S. marianum </em>to pregnant woman.
Silybum marianum,Silymarin,Mouse fetus,Teratogenicity
https://ajp.mums.ac.ir/article_6679.html
https://ajp.mums.ac.ir/article_6679_ce7ede7d31c2b6c70c9c064933385c8a.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
Comparison of antiplatelet activity of garlic tablets with cardio-protective dose of aspirin in healthy volunteers: a randomized clinical trial
550
557
EN
Mojtaba
Shafiekhani
Department of Pharmacotherapy, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
mshafikhan@sums.ac.ir
Pouya
Faridi
Department of phytopharmaceuticals , Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
faridi@sums.ac.ir
Javad
Kojuri
Department of Cardiology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
kojurij@yahoo.com
Soha
Namazi
Department of Pharmacotherapy, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
namazisoha@yahoo.com
10.22038/ajp.2016.6680
<br/><strong>Objective: </strong>Some of the adverse effects of aspirin including peptic ulcers, gastrointestinal bleeding and aspirin resistance compelled researchers to find a suitable alternative with fewer adverse effects. In this clinical trial, we aimed to find the effective antiplatelet dose of garlic. <br/><strong>Materials and Methods:</strong> This randomized controlled clinical trial (RCT) was conducted on 62 healthy volunteers of 20-50 years old. All volunteers used 80 mg aspirin per day for 1 week and at the end of this time, platelet aggregation (PA) induced by 4 agonists acting in aggregation pathway including adenosinediphosphate (20 μmol/l), epinephrine (20 μmol/l), collagen(0.19 mg/ ml) and arachidonic acid (0.5mg/ ml) was measured by Light Transmittance Aggregometry (LTA) in all participants. After one month washout period, volunteers were randomized into 3 groups and each received 1, 2 or 3 garlic tablets (1250 mg) a day for 1 month. After one month, PA was examined in all groups. <br/><strong>Results:</strong> The mean ±SD of the age of all volunteers was 28.60 ± 9.00 years. In addition, 52.00 % of our volunteers were male and 48.00% of them were female. Garlic tablet didnot have significant effect on PA at any dose. However, 30% of volunteers in the group that used 3 garlic tablets/day reported adverse effect (i.e. bleeding). No significant association between sex, age and PA was observed. <br/><strong>Conclusion:</strong> In this study, we were unable to determine the effective anti-platelet dose of garlic which that could be equal to that of aspirin anti-platelet activity, as assessed LTA method.
Garlic,Aspirin,Anti-Platelet,Light transmittance aggregometry
https://ajp.mums.ac.ir/article_6680.html
https://ajp.mums.ac.ir/article_6680_e1e9b90f31fbf8eabe20cc4c3715da1d.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
Evaluation of acute and sub-acute toxicity of Pinus eldarica bark extract in Wistar rats
558
566
EN
Akram
Ghadirkhomi
Department of Pharmacology, Islamic Azad University, Shahreza Branch, Shahreza, Iran
ghdrkhm@yahoo.com
Leila
Safaeian
Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
leila_safaeian@pharm.mui.ac.ir
Behzad
Zolfaghari
0000-0001-7087-2590
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
behzadz@gmail.com
Mohammad Reza
Aghaye-Ghazvini
Isfahan Center of Public Health Training and Research, Institute of Public Health Research, Tehran University of Medical Science, Iran
mr-ghazvini@farabi.tums.ac.ir
Parisa
Rezaei
Department of Pathology, Seyed-Al-Shohada Hospital, Isfahan, Iran
lsafaeian@yahoo.com
10.22038/ajp.2016.6706
<br/><strong>Objective: </strong><em>Pinus eldarica</em> (<em>P. eldarica</em>) is one of the most common pines in Iran which has various bioactive constituents and different uses in traditional medicine. Since there is no documented evidence for <em>P. eldarica</em> safety, the acute and sub-acute oral toxicities of hydroalcoholic extract of<em> P. eldarica</em> bark were investigated in male and female Wistar rats in this study. <br/><strong>Materials and Methods:</strong> In the acute study, a single dose of extract (2000 mg/kg) was orally administered and animals were monitored for 7 days. In the sub-acute study, repeated doses (125, 250 and 500 mg/kg/day) of the extract were administered for 28 days and biochemical, hematological and histopathological parameters were evaluated. <br/><strong>Results: </strong>Our results showed no sign of toxicity and no mortality after single or repeated administration of <em>P. eldarica.</em> The median lethal dose (LD<sub>50</sub>) of <em>P. eldarica </em>was determined to be higher than 2000 mg/kg. The mean body weight and most of the biochemical and hematological parameters showed normal levels. There were only significant decreases in serum triglyceride levels at the doses of 250 and 500 mg/kg of the extract in male rats (p<br/><strong>Conclusion:</strong> Oral administration of the hydroalcoholic extract of<em> P. eldarica</em> bark may be considered as relatively non-toxic particularly at the doses of 125 and 250 mg/kg.
Pinus eldarica,Toxicity,Hematology,Serum biochemistry,Histopathology
https://ajp.mums.ac.ir/article_6706.html
https://ajp.mums.ac.ir/article_6706_62a47ae6331350f3e08c8dde58be32ee.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
The effect of nano-curcumin on HbA1c, fasting blood glucose, and lipid profile in diabetic subjects: a randomized clinical trial
567
577
EN
Hamid Reza
Rahimi
0000-0002-2269-4109
Student Research Committee, Department of Modern Sciences & Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
rahimihr@mums.ac.ir
Amir Hooshang
Mohammadpour
0000-0002-6000-3276
Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
mohamadpoorah@mums.ac.ir
Mostafa
Dastani
0000-0003-2788-2827
Cardiovascular Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
dastanim@mums.ac.ir
Mahmoud Reza
Jaafari
0000-0003-3908-6828
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Science, Mashhad, Iran Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Science, Mashhad, Iran
jafarimr@mums.ac.ir
Khali
Abnous
Pharmaceutical Research Center, Department of Medicinal Chemistry, Mashhad University of Medical Sciences, Mashhad, Iran
abnouskh@mums.ac.ir
Majid
Ghayour Mobarhan
0000-0003-2852-7632
Cardiovascular Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
ghayourm@mums.ac.ir
Reza
Kazemi Oskuee
Department of Medical Biotechnology, Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
oskueekr@mums.ac.ir
10.22038/ajp.2016.6761
<br/><strong>Objective: </strong>Diabetes mellitus is defined as a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both or insulin resistance. Curcumin inhibits NF-κB signaling pathway. The aim of this study is evaluation of the effect of Nano-curcumin on HbA1C, fast blood glucose and lipid profile in diabetic patients. <br/><strong>Materials and Methods: </strong>Seventy type-2 diabetic patients (fasting blood glucose (FBG) ≥ 126 mg/dL or 2-hr postprandial blood glucose ≥200 mg/dl) randomly receivedeither Curcumin (as nano-micelle 80 mg/day) or placebo for 3 months in a double blind randomized clinical trial. Fasting blood glucose, HbA1C, and lipids profile were checked before and after the intervention. Data analyses, including parametric and nonparametric tests were done using the SPSS 11.5 software. A p value < 0.05 was regarded as statistically significant. (RCT registration code: IRCT2013081114330N1) <br/><strong>Results: </strong>Mean age, BMI, FBG, total cholesterol (TC), triglyceride (TG), LDL, HDL, HbA1c , and sex and had no significant difference at the baseline between the groups. In Nano-curcumin group, a significant decrease was found in HbA1C, FBG, TG, and BMI comparing results of each subject before and after the treatment (p<0.05). <br/>By comparing pre- and post-treatment values among the groups, HbA1c, eAG, LDL-C, and BMI variables showed significant differences (p<0.05). <br/><strong>Conclusion:</strong> These findings suggest an HbA1c lowering effect for Nano-curcumin in type-2 diabetes; also, it is partially decrease in serum LDL-C and BMI.
Curcumin,HbA1c,Fast blood glucose,lipid profile
https://ajp.mums.ac.ir/article_6761.html
https://ajp.mums.ac.ir/article_6761_be0998e76887a36c2c29345d61f818e6.pdf
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
6
5
2016
09
01
Effects of flavonoids from Martynia annua and Tephrosia purpurea on cutaneous wound healing
578
591
EN
Santram
Lodhi
Department of Pharmaceutical Sciences,
Dr. H. S. Gour University,
Sagar, Madhya Pradesh, India
srlodhi78@gmail.com
Avijeet
Jain
Department of Pharmacy
RKDF University, Bhopal
Madhya Pradesh, India-462033
avijeet_9826275340@rediffmail.com
Alok Pal
Jain
Department of Pharmacy, Guru Ramdas Khalsa Institute of Science & Technology, Kukrikheda, Barela, Jabalpur (M.P.), India
dralokpaljain@gmail.com
Rajesh
Pawar
Department of Pharmacognosy,
VNS Institute of Pharmacy, Bhopal, Madhya Pradesh, India
rajeshabc14@rediffmail.com
Abhay Kumar
Singhai
Department of Pharmaceutical Sciences,
Dr. H. S. Gour University,
Sagar, M.P., 470003. India
abhayksinghai@yahoo.co.in
10.22038/ajp.2016.6760
<br/><strong>Objective: </strong><em>Martynia annua</em> L. (<em>M. annua</em>), (Martyniaccae) has been traditionally used in the treatment of epilepsy, sore throat and inflammatory disorders. The leaf paste is used topically on Tuberculosis of the lymphatic glands and wounds of domestic animals. <em>Tephrosia purpurea </em>(<em>T. purpurea</em>), (Fabaceae) has been used traditionally as a remedy for asthma, gonorrhea, rheumatism and ulcers. This study aimed to evaluate the potential wound healing effects of different fractions ofethanol extract of <em>M. annua</em> leaves and aerial parts of <em>T. purpurea</em>. <br/><strong>Materials and Methods:</strong> Methanol fraction of <em>M. annua</em> (MAF-C) and ethyl acetate fraction of <em>T. purpurea</em> (TPF-A) were evaluated for healing potential in dead-space and burn wound models. An ointment (5% w/w) of MAF-C and TPF-A, pongamol (0.2 and 0.5% w/w) and luteolin (0.2 and 0.5% w/w) was applied topically twice a day. The effects were compared with Povidone Iodine ointment with respect to protein, collagen content, enzymatic assay and histopathological finding of granuloma tissues. <br/><strong>Results:</strong> Ethanol extracts of <em>M. annua</em> and <em>T. purpurea</em>were exhibited total flavonoid contents of 126.2 ± 4.69 and 171.6 ± 6.38 mg (quercetin equivalent), respectively. HPLC fingerprinting confirmed the presence of luteolin in<em> M. annua </em>and quercetin in <em>T. purpurea</em>. TPF-A and MAF-C ointments (5% w/w) significantly increases the hydroxyproline and protein contents. Luteolin and pongamol ointments were also found to be effective in both wound models. <br/><strong>Conclusion:</strong> Our findings suggested that 5% w/w ointment of TPF-A and MAF-C fractions were more effective than isolated flavonoids in wound healing which may be due to synergistic interactions between the flavonoids and other constituents.
Martynia annua,Tephrosia purpurea,Povidone Iodine ointment,Burn wound,Luteolin,Dead space wound
https://ajp.mums.ac.ir/article_6760.html
https://ajp.mums.ac.ir/article_6760_7a4e912759a2aa5734383791db4a4ac0.pdf