ORIGINAL_ARTICLE
The effect of celery (Apium graveolens L.) on reproductive parameters in male wistar rat
Dear editor In recent years, the number of scientific research papers of Iranian scientists has substantially grown in national and international journals that indicates particular attitude of Iranian scientific community to the development of knowledge in different fields. Moreover, improvement of quality of scientific papers is necessary. For this purpose, criticism of published studies is a way to increase the quality of articles and make them clear. In Avicenna Journal of Phytomedicine, volume (5), issue (2), year 2015, an article entitled “Effects of aqueous extract of celery (Apium graveolens L.) leaves on spermatogenesis in healthy male rats” was published and the papers like this should be appreciated. However, the paper has some drawbacks which if not resolve, could be misleading for researchers who tend to use it or do research in its direction. So, with all due respect to the research team, we decided to evaluate the paper ambiguities in order to improve the quality of future articles.
https://ajp.mums.ac.ir/article_6189_3f26ee44714dd12fd1de8aa7db56007a.pdf
2016-05-01
260
265
10.22038/ajp.2016.6189
Wesam
Kooti
wesamkooti@gmail.com
1
Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
AUTHOR
Najmeh
Kafash-Farkhad
kafash_embriology@yahoo.com
2
Department of Biology, Urmia University, Urmia, Iran
AUTHOR
Ali
Ghorbani-Ranjbari
dr_alighorbani87@yahoo.com
3
Department of Biotechnology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
LEAD_AUTHOR
Naim
Sharafi-Ahvazi
aliakbarisara_70@yahoo.com
4
Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
AUTHOR
Kerishchi P, Nasri S, Amin G, Tabibian M, editors. The effects of Apium graveolens extract on sperm parameters and H-G hormonal axis in mice. Proceedings of the 20th Iranian Congress of Physiology and Pharmacology; 2011 Sep; Hamedan, Iran
1
Ghasemiboroon M, Ghafourian Boroujerdnia M, Ahangarpoor A, Kooti W, Hasanzadeh Noohi Z, Noori Ahmad Abadi M. 2014. The effect of hydroalcoholic extract of Celery (Apium graveolens) leaves on serum level of
2
testosterone, FSH and LH in Male Rats. ZUMS Journal, 93:49-57
3
Mokhtari M, Zanboori M. 2011. The Effects of Lead Acetate on Sexual Behavior and the Level of Testosterone in Adult Male Rats. Int J Fertil Steril, 1: 13-20.
4
Hamza AA, Amin A. 2007. Apium graveolens modulates sodium valproate‐induced reproductive toxicity in rats. J Exp Zool A Ecol Genet Physiol, 4: 199-206.
5
Freitas F, Cordeiro-Mori F, Sasso-Cerri E, Lucas S, Miraglia S. 2002. Alterations of spermatogenesis in etoposide-treated rats: a stereological study. Interciencia, 1:227–35.
6
Momen HR, Eskandari N. 2012. Effect of vitamin E on sperm parameters and DNA integrity in sodium arsenite-treated rats. Iran J Reprod Med, 3: 249–56.
7
Kooti W, Mansouri E, Ghasemiboroon M, Harizi M, Ashtary-Larky D, Afrisham R. 2014. The Effects of hydroalcoholic extract of apium graveolens leaf on the number of sexual cells and testicular structure in rat. Jundishapur J Nat Pharm Prod, 4: e17532.
8
Afzalzadeh MR, Papahn AA, Amirzargar A, Kazemi Varnamkhasti M, Ganjali H, Gharib Mombeni E. 2013. Effect of Vitis ViniferaLeave Hydro-Alcoholic Extract on Reproductive Parameters in Adult Normal Male Rats. J Phys Pharm Adv, 6: 159-166.
9
Ghorbani Ranjbary A, Ghorbani Ranjbary N, Ghorbani Ranjbary Z, Jouibar F. 2014. Effects of intraperitoneal injection of extracts of origanum vulgare on gonadotropin and testosterone hormones in male Wistar rats. J Babol Univ Med Sci, 4: 57-63.
10
Shalizar Jalali A, Hasanzadeh SH. 2013. Crataegus monogyna fruit aqueous extract as a protective agent against doxorubicin-induced reproductive toxicity in male rats. Avicenna Journal of Phytomedicine, 2: 159-170.
11
Dorostghoal M, Seyyednejad SM, Khajehpour L, Jabari A. 2013. Effects of Fumaria parviflora leaves extract on reproductive parameters in adult male rats. Iran J Reprod Med, 11: 891-8.
12
Li H, Li HB, Zhang M, Yan F, Zhang ZX, Li ZL. 2010. Effect of apigenin on the reproductive system in male mice. Health, 5: 435-40.
13
Ohlsson A, Ulleras E, Cedergreen N, Oskarsson A. 2010. Mixture effects of dietary flavonoids on steroid hormone synthesis in the human adrenocortical H295R cell line. Food Chem Toxicol, 11: 3194-200.
14
Kooti W, Ghasemiboroon M, Ahangarpoor A, Hardani A, Amirzargar A, Asadi-Samani M. 2014. The effect of hydro-alcoholic extract of celery on male rats in fertility control and sex ratio of rat offspring. J Babol Univ Med Sci, 4: 43-49.
15
ORIGINAL_ARTICLE
Phytochemical and pharmacological aspects of Descurainia sophia Webb ex Prantl: modern and traditional applications
Seed of Descurainia sophia Webb ex Prantl has been traditionally prescribed as treatment for palpitation, varicose vein, varicocele, constipation, hemorrhoid, skin eruptions, and impotence. To outline a view for further approaches, current work compiled a survey on all relevant clinical properties of this medicament in addition to the traditional reports. To do this, databases as PubMed, Scopus, EMBASE, IranMedex and Science information databases (SID) were searched by keywords, i.e., “Descurainia sophia”, “Khaksheer”, and “Flixweed” as well as “pharmacology” and “phytochemistry”. According to the findings, scant experimental evaluation and clinical assessment have been performed on this medicament. Of those, only anti-inflammatory, analgesic, and antipyretic effects as well as antioxidant and anthelmintic activities were assessed and confirmed in experimental studies. Despite broad administration of this herb in folk and traditional medicine, only two human clinical trials in bowel discomfort and pregnant subjects were conducted. Taken as a whole, more comprehensive clinical evaluations should be conducted on respective applications to support those traditional and folk uses.
https://ajp.mums.ac.ir/article_4469_77af9d8fed7874998fb2ce14034a248c.pdf
2016-05-01
266
272
10.22038/ajp.2016.4469
Descurainia sophia Webb ex Prantl
Pharmacology
Phytochemistry
Traditional Medicine
Majid
Nimrouzi
nimruzim@sums.ac.ir
1
Research Institute for Islamic and Complementary Medicine, Iran University of Medical Sciences, Tehran, Iran
AUTHOR
Mohammad Mahdi
Zarshenas
zarm@sums.ac.ir
2
Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
LEAD_AUTHOR
Ahmadieh A. 2007. Raz-e-Darman(Secret of treatment), pp.171-172, Tehran, Iran, Eghbal press (In Persian).
1
Akbari N, Parvin N, Sereshti M, Safdari F. 2011. Study about different types of medicinal plants used by elderly people in Shahrekord city. J Shahrekord Univ Med Sci, 12: 26-32.
2
Arzani MA. 1870. Mīzān al-Tibb(Handbook of medicine for beginners), p. 238, India, Nūl Kishūr press (In Persian).
3
Arzani MA. 1915. Mofareh Al-Gholub, pp. 103 Lahoor, Salim lahoor press (In Persian).
4
yoobi F, Kamali B, Shamsizadeh A, Sajadi MA, Roohbakhsh A, Vazirinejad R, Hakimi E, Rezazadeh HA, Rahmani MR, Allahtavakili M. 2013. Effect of Aqueous Extract of Descurainia Sophia on Castor Oil-Induced Diarrhea in Male Rat. J Rafsenjan Univ Med Sci, 12: 149-156.
5
Behbahani MS, Abbasi S, Using shear reversible gels to stabilize the Flixweed (Descurainia sophia L.) syrup. 2013, 1stInternational e-Conference on novel Food Processing, Mashhad, Iran.
6
Buck WB, Osweiter GD, Van Gelder AG. 1976. Clinical and diagnostic veterinary toxicology. 2nd Ed. Iowa, Kendall/ Hunt Publishing Company.
7
Mirheidar H. 2005. Maaref giahi (Plant Knowledge), pp. 172-173, Tehran, Iran, Daftare Nashre Farhange Eslami.
8
Hossaini-Tabib, M. 1959. Tuhfat al-mu'minīn(Present for the faithful), pp. 342-346 Tehran and Qum, Iran, Mostafavi Press (In Persian).
9
Kai S, Xian L. 2003. Study on the chemical constituents of Descurainia sophia (L.) Webb ex Prantl. Shenyang Yao Ke Da Xue Xue Bao (Journal of Shenyang Pharmaceutical University), 6: 8-8.
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Khorasani MA. 2001. Makhzan al Advieh (The storehouse of medicaments), p. 351, Research institute for Islamic and Complementary Medicine, Iran University of Medical Sciences, Tehran, Iran, Bavardaran Press (In Persian).
11
Lee YJ, Kim NS, Kim H, Yi JM, Oh SM, Bang OS, Lee J. 2013. Cytotoxic and anti-inflammatory constituents from the seeds of Descurainia sophia. Arch Pharm Res, 36: 536-541.
12
Li J, Liu X, Dong F, Xu J, Zheng Y, Shan W. 2010. Determination of the volatile composition in essential oil of Descurainia sophia (L.) Webb ex Prantl (Flixweed) by gas chromatography/mass spectrometry (GC/MS). Molecules, 15: 233-240.
13
Li W, Liu X, Khan MA, Kamiya Y, Yamaguchi S. 2005. Hormonal and environmental regulation of seed germination in flixweed (Descurainia sophia). Plant Growth Regul, 45: 199-207.
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Maraghi S, Torfi Jabrpoor N. 2002. Study of in vitro and in vivo effects of Descurainia sophia extract on Hymenolepis nana in comparison with niclosamide. Hakim, 5: 57-62.
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Mirzaei A, Mohammadi J, Mirzaei N, Mirzaei M. 2011. The Antioxidant Capacities and Total Phenolic Contents of Some Medicinal Plants in Iran. J Fasa Univ Med Sci, 1: 160-167.
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Mohamed NH, Mahrous AE. 2009. Chemical constituents of Descurainia sophia L. and its biological activity. Rec Nat Prod, 3: 58-67.
17
Mohammadinia N, Rezaei MA, Loripour M, Heydari N. 2012. The Effect of Consumption of Sisymbrium-Seeds at the End of Pregnancy on the Rate of Cesarean Delivery and Apgar Score. Iran J Obstet Gynecol Infertil, 15: 8-13.
18
Pasalar M, Bagheri Lankarani K, Mehrabani D, Tolidei HR, Naseri M. 2013. The effect of Descureania Sophia L. and Prunus Domestica L. in prevention of constipation among Iranian Hajj Pilgrims, Saudi Arabia. Res J Pharm Biol Chem Sci, 4: 1195-1204.
19
Peng L, Yi Y, Fu-li G, Ze-qü L. 1997. A preliminary study on the introduction of Descurainia sophia, an oil plant species for industrial uses. Acta Botanica Sinica, 39: 447-479.
20
Sun K, Li X, Li W, Liu JM, Wang JH, Sha Y. 2006. A new nor-lignan from the seeds of Descurainia sophia. Nat Prod Res, 20: 519-522.
21
Sun K, Li X, Li W, Wang J, Liu J, Sha Y. 2004. Two new lactones and one new aryl-8-oxa-bicyclo[3,2,1]oct-3-en-2-one from Descurainia sophia. Chem Pharm Bull (Tokyo), 52: 1483-1486.
22
Sun K, Li X, Liu JM, Wang JH, Li W, Sha Y. 2005. A novel sulphur glycoside from the seeds of Descurainia sophia (L.). J Asian Nat Prod Res, 7: 853-856.
23
Tavakoli R, Mohadjerani M, Hosseinzadeh R, Tajbakhsh M, Naqinezhad A 2012. Chemical Composition of Fatty Acid from Different Parts of Descurainia Sophia L. Growing Wild in North of Iran. Anal Chem Lett, 2: 363-366.
24
Wang AQ, Wang XK, Li JL, Cui XY. 2004. Isolation and structure identification of chemical constituents from the seeds of Descurainia sophia (L.) Webb ex Prantl. Yao Xue Xue Bao (Acta Pharmaceutica Sinica), 39: 46-51.
25
Zarshenas MM, Petramfar P, Firoozabadi A, Moein MR, Mohagheghzadeh, A. 2013. Types of headache and those remedies in traditional persian medicine. Pharmacogn Rev, 7: 17-26.
26
Zhongfeng X. 2007. Determination of Trace Elements in Descurainia sophia by FAAS. Anhui Nong Ye Ke Xue (Journal of Anhui Agricultural Sciences), 35: 10575.
27
ORIGINAL_ARTICLE
Effects of flaxseed and Hypericum perforatum on hot flash, vaginal atrophy and estrogen-dependent cancers in menopausal women: a systematic review and meta-analysis
Objective: In this study, we aimed at evaluation of the efficacy of Hypericum perforatum and flaxseed on hot flash, vaginal atrophy and estrogen-dependent cancers in menopausal women
Materials and Methods: We searched MEDLINE, Scopus, and the Cochrane Central Register of Controlled Trials (RCT) to explore trials that assessed the effectiveness of H. perforatum and flaxseed on hot flash, vaginal atrophy and estrogen-dependent cancers. In this regard, the following terms were used “menopause AND H. perforatum OR flaxseed OR Linum usitatissimum. Only randomized controlled trials were included in the study.
Results: Nine RCTs were included in this systematic review. Based on the literature, flaxseed showed beneficial effect on hot flash frequency and intensity, which was not statistically significant. According to two trials, flaxseed showed estrogenic effects; however, no conclusion regarding cancer promoting or protecting effects can be made. The evidence of the efficacy of the flaxseed on alleviating vaginal atrophy was also limited due to inconsistent findings in this regard. One trial declared that Vitex agnus-castus and H. perforatum showed comparable decrease in the frequency of hot flashes.
Conclusion: The results of our systematic review suggest beneficial effect on vasomotor symptom with both of flaxseed and H. perforatum. Consistent conclusion regarding estrogen-dependent cancers and maturation value is limited due to small number of trials related to flaxseed. Further trials are still needed to confirm the results of our systematic review.
https://ajp.mums.ac.ir/article_5331_f836bb0d8bccd74b9c2cc3c43b5e5fa3.pdf
2016-05-01
273
283
10.22038/ajp.2016.5331
Hot flash
Vaginal atrophy
Menopause
Systematic review
Cancers
Masumeh
Ghazanfarpour
ghazanfarpm901@mums.ac.ir
1
Student Research Committee, Department of Midwifery and Reproductive Health, Nursing and Midwifery School, Mashhad University of Medical Science, Mashhad, Iran
AUTHOR
Ramin
Sadeghi
sadeghir@mums.ac.ir
2
Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Robab
Latifnejad Roudsari
latifnejadr@mums.ac.ir
3
Evidence-Based Care Research Centre, Department of Midwifery, School of Nursing and Midwifery, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Talat
Khadivzadeh
goli.yasaman@yahoo.com
4
Department of Midwifery, School of Nursing and Midwifery, Mashhad University of Medical Sciences, Mashhad, Iran Mashhad, Iran
LEAD_AUTHOR
Imaneh
khorsand
i.khorsand@gmail.com
5
Department of Microbiology, Islamic Azad University of Varamin-pishva,Tehran, Iran
AUTHOR
Maliheh
Afiat
afiatm@mums.ac.ir
6
Obstetrics and Gynecology, Women's Health Research Center, Department of Obstetrics and Gynecology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mahdi
Esmaeilizadeh
mahdiesmaeilizadeh@gmail.com
7
Esfarayen faculty of Medical Sciences, Esfarayen, Iran
AUTHOR
Avis NE, Stellato R, Crawford S, Johannes C, Longcope C. 2000. Is there an association between menopause status and sexual functioning?.Menopause, 7: 297-309.
1
Baber RJ, Templeman C, Morton T, Kelly GE, West L.1999. Randomized placebo-controlled trial of an isoflavone supplement and menopausal symptoms in women. Climacteric, 2: 85-92.
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Briese V, Stammwitz U, Friede M, Henneicke-von Zepelin HH. 2007. Black cohosh with or without St. John's wort for symptom-specific climacteric treatment—Results of a large-scale, controlled, observational study. Maturitas, 57: 405-414.
3
Buck K, Zaineddin AK, Vrieling A, Linseisen J, Chang-Claude J. 2010. Meta-analyses of lignans and enterolignans in relation to breast cancer risk. Am J Clin Nutr, 92: 141-153.
4
Chung DJ, Kim HY, Park KH, Jeong KA, Lee SK, Lee YI, Hur SE, Cho MS, Lee BS, Bai SW, Kim CM, Cho SH, Hwang JY, Park JH. 2007. Black Cohosh and St. John's wort (GYNO-Plus®) for climacteric symptoms. Yonsei Med , 48: 289-294.
5
Colli MC, Bracht A, Soares AA, de Oliveira AL, Bôer CG, de Souza CG, Peralta RM. 2012. Evaluation of the Efficacy of Flaxseed Meal and Flaxseed Extract in Reducing Menopausal Symptoms. J Med Food, 15: 840-845.
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12
Ghazanfarpour M, Ghaderi E, Kaviani M, Haddaian K. 2013. Comparison the efficacy of Hypericum perforatum and vitex agnus-castus in hot flashes: A double-blinded randomized controlled trial. Chron Dis J, 1: 67-73.
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Anderson G, Cummings S, Freedman LS, et al. 1998. Design of the women’s health initiative clinical trial and observational study. Control Clin Trials, 19:61–109.
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Hidalgo LA, Chedraui PA, Morocho N, Ross S, San Miguel G. 2005. The effect of red clover isoflavones on menopausal symptoms, lipids and vaginal cytology in menopausal women: a randomized, double-blind, placebo-controlled study. Gynecol Endocrinol, 21: 257-64.
15
Hooper L, Ryder JJ, Kurzer MS, Lampe JW, Messina MJ, Phipps WR, Cassidy A. 2009. Effects of soy protein and isoflavones on circulating hormone concentrations in pre-and post-menopausal women: a systematic review and meta-analysis. Hum Reprod Update, 15: 423-440.
16
Lewis JE, Nickell LA, Thompson LU, Szalai JP, Kiss A, Hilditch JR. 2006. A randomized controlled trial of the effect of dietary soy and flaxseed muffins on quality of life and hot flashes during menopause. Menopause, 13: 631-42.
17
Lipovac M, Chedraui P, Gruenhut C, Gocan A, Kurz C, Neuber B, Imhof M. 2012. The effect of red clover isoflavone supplementation over vasomotor and menopausal symptoms in postmenopausal women. Gynecol Endocrinol, 28: 203-7.
18
Loprinzi CL, Kugler JW, Sloan JA, Mailliard JA, LaVasseur BI, Barton DL, Novotny PJ, Dakhil SR, Rodger K, Rummans TA, Christensen BJ. 2000. Venlafaxine in management of hot flashes in survivors of breast cancer: a randomised controlled trial. Lancet, 356: 2059-2063.
19
Lowcock EC, Cotterchio M, Boucher BA. 2013. Consumption of flaxseed, a rich source of lignans, is associated with reduced breast cancer risk. Cancer Causes Control, 24:813-6.
20
Maclennan AH, Broadbent JL, Lester S, Moore V. 2004. Oral oestrogen and combined oestrogen/progestogen therapy versus placebo for hot flashes. Cochrane Database Syst Rev, 4.
21
Pruthi S, Qin R, Terstreip SA, Liu H, Loprinzi CL, Shah TR, Tucker KF, Dakhil SR, Bury MJ, Carolla RL, Steen PD, Vuky J, Barton DL. 2012. A phase III, randomized, placebo-controlled, double-blind trial of flaxseed for the treatment of hot flashes: North Central Cancer Treatment Group N08C7. Menopause, 19: 48-53.
22
Simbalista RL, Sauerbronn AV, Aldrighi JM, Arêas JA. 2010. Consumption of a flaxseed-rich food is not more effective than a placebo in alleviating the climacteric symptoms of postmenopausal women. J Nutr, 140: 293-7.
23
Uebelhack R, Blohmer JU, Graubaum HJ, Busch R, Gruenwald J, Wernecke KD. 2006. Black cohosh and St. John's wort for climacteric complaints: a randomized trial. Obstet Gynecol, 107: 247-255.
24
van Die MD, Burger HG, Bone KM, Cohen MM, Teede HJ. 2009. Hypericum perforatum with Vitex agnus-castus in menopausal symptoms: a randomized, controlled trial. Menopause, 16:156-163.
25
ORIGINAL_ARTICLE
Anti-inflammatory and antinociceptive activities of Solenostemon monostachyus aerial part extract in mice
Objective: Solenostemon monostachyus is used in traditional medicine for the treatment of various ailments such as ulcer, hypertension, pains and inflammatory diseases. Evaluation of anti-inflammatory and analgesic activities of S. monostachyus aerial parts was carried out to ascertain its uses in traditional medicine. Materials and Methods: The aerial parts of S. monostachyus was cold extracted by soaking the dried powdered material in ethanol. The aerial parts crude extract (75 –225 mg/kg) of S. monostachyus was investigated for analgesic and anti-inflammatory activities using various experimental models; acetic acid, formalin and thermal- induced pains models for analgesic study and carrageenin, egg albumin and xylene – induced edema models for anti-inflammatory investigation. Results: The extract caused a significant (pConclusion: The anti-inflammatory and analgesic effects of this plant may in part be mediated through the chemical constituents of the plant and the results of the analgesic action suggest central and peripheral mechanisms. The findings of this work confirm the ethno medical use of this plant to treat inflammatory conditions.
https://ajp.mums.ac.ir/article_5485_e8fc1ac326bc75d4ee2443dc4b3db959.pdf
2016-05-01
284
294
10.22038/ajp.2016.5485
Solenostemon monostachyus
Antiinflammatory
Analgesic
Jude Fiom
Okokon
judeefiom@yahoo.com
1
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria
LEAD_AUTHOR
Koofreh
Davis
koofrehdavis@yahoo.com
2
Department of Physiology, Faculty of Basic Medical Sciences, University of Uyo, Uyo, Nigeria
AUTHOR
Lucky Legbosi
Nwidu
menelucky@yahoo.com
3
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa State
AUTHOR
Adebayo JO, Krettli AU. 2011. Potential antimalarials from Nigerian plants: A review. J Ethnopharmacol, 133: 289 –302.
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Ahmed M, Sadhu SK, Datta BK, Kunu JK, Bachar SC.1997. Preliminary studies on the antiinflammatory, analgesic and diuretic activity of stagninol, a sesquiterpene isolated from Persicaria stagnina. Pharmazie, 52:472-475.
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Ajibesin KK, Ekpo BA, Bala DN, Essien EE, Adesanya SA. 2008. Ethnobotanical survey of Akwa Ibom State of Nigeria. J Ethnopharmacol, 115: 387 – 408.
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Akah PA, Nwanbie A. 1994. Evaluation of Nigerian traditional medicines plants used for rheumatic (inflammatory) disorder. J Ethnopharmacol, 42: 179 – 182.
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Amazu LU, Antia BS, Okokon JE. 2015. Antiulcer activity of S. monostachyus. The J Phytopharmacol. 4: 97 – 101
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Berken T, Ostunes L, Lermioglu F, Ozer A. 1991. Antiinflammatory analgesic and antipyretic effect of an aqueous extract of Erythraea ceulaurum. Planta Med, 57: 34 -37.
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62
ORIGINAL_ARTICLE
Evaluation of wound healing, antioxidant and antimicrobial efficacy of Jasminum auriculatum Vahl. leaves
Objective: To validate the ethno-therapeutic claim of the traditionally used plant Jasminum auriculatum (J. auriculatum) in skin diseases, by evaluating its wound healing potential along with its antioxidant and antimicrobial properties; so as to understand their role in wound healing. Materials and Methods: Excision and incision wound models were used to evaluate the wound healing activity on albino rats. The wound healing potential was assessed by measuring rate of wound contraction, epithelialization period, hydroxyproline content, skin breaking strength and histopathological parameters. Reference standard drug was Nitrofurazone ointment. The antioxidant activity was determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method. The antimicrobial activity was determined by agar well diffusion method and minimum inhibitory concentration by serial dilution method. Results: Higher rate of wound contraction (83.66±0.50% on 15th day), decrease in the period of epithelialization (17.83±1.6days), higher skin breaking strength (170.71±1.52g), higher collagen content and favourable histopathological changes revealed that topical application of ointment containing successive ethanolic extract (S.E.E) of J. auriculatum leaves has the most potent wound healing ability compared to control group in both the models studied. The DPPH radical scavenging activity of successive ethanolic extract was found to be 33.39µg/ml. Successive ethanolic extract was found to be most effective against Pseudomonas auregenosa having a zone of inhibition 16.65±0.6mm and the minimum inhibitory concentration was 0.78mg/ml. Conclusion: The data of this study indicate that successive ethanolic extract of the leaves exhibit potent wound healing, antioxidant and antimicrobial properties. This justifies the ethno-medicinal use of plant for the treatment of wound and microbial infections.
https://ajp.mums.ac.ir/article_5723_84e7149136a53a04a2fa7f2ce796a2ab.pdf
2016-05-01
295
304
10.22038/ajp.2016.5723
Jasminum auriculatum
Excision
Incision
Hydroxyproline content
Skin breaking strength
Antioxidant activity
Antimicrobial activity
Arun
Mittal
mittalarun07@rediffmail.com
1
Department of Pharmacognosy and Phytochemistry. Hindu College of Pharmacy, Sonipat, Haryana-131001, India
LEAD_AUTHOR
Sardana
Satish
2
Department of Pharmacognosy and Phytochemistry. Hindu College of Pharmacy, Sonipat, Haryana-131001, India
AUTHOR
Pandey
Anima
3
Department of Pharmaceutical Sciences. Birla Institute of Technology,Mesra, Jharkhand-835215, India
AUTHOR
Abd-Elsalam KA, Aly NI, Abdel-Satar AM, Khalil SM, Verreet AJ.2013. PCR identification of Fusarium genus based on nuclear ribosomal-DNA sequence data. African J Biotech, 2: 82-85.
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3
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38
ORIGINAL_ARTICLE
Anticonvulsant effect of Satureja hortensis L. aerial parts extracts in mice
Objective: Regarding the anticonvulsant effects of Satureja hortensis (S. hortensis) in Avicenna’s book: canon of medicine; the present study was undertaken to evaluate the anti- eplileptic effects of S. hortensis aqueous and ethanolic aerial part extracts. Furthermore, the mechanisms of their anticonvulsant activities were also evaluated.
Materials and Methods: Seizure was induced by Pentylentetrazol (PTZ) and MES (maximal electroshock) models. Mice were randomly divided into 8 groups; negative control (normal saline, 10ml/Kg), positive control (diazepam, 2 mg/kg), S. hortensis aqueous and ethanolic extracts (200, 400 and 600 mg/kg). In PTZ test, latency to the first minimal clonic seizure (MCS), latency to the first generalized tonic–clonic seizures (GTCS), the total duration of seizures and protection against mortality were evaluated. In MES test, the stretching length of extremities and protection against mortality were recorded.
Results: Aqueous and ethanolic extracts (400 and 600 mg/kg) significantly increased MCS and GTCS latencies in PTZ model. Three doses of the extracts decreased the total duration of seizure. These extracts did not show any protective effects on seizure induced by MES model. In PTZ model, flumazenil, an antagonist of benzodiazepine (BZD) site in the GABAA-BZD receptor complex and 7- nitroindazole (7- NI), a selective nNOS (neuronal nitric oxide synthase) inhibitor, reduced the prolongation of seizure latency.
Conclusion: S. hortensis showed anticonvulsant activity in PTZ model and this effect may be mediated, at least partly, through interacting with nitric oxide and GABAA-BZD receptor complex.
https://ajp.mums.ac.ir/article_5273_d67db7afbdc148221ede280c15e535b9.pdf
2016-05-01
305
312
10.22038/ajp.2016.5273
Satureja hortensis
Seizure
Pentylenetetrazol
Maximal electroshock
Flumazenil
7-nitro- indazole
Farzaneh
Zolfagharian
1
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Bibi Marjan
Razavi
razavimr@mums.ac.ir
2
Targeted Drug Delivery Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Hossein
Hosseinzadeh
hosseinzadehh@mums.ac.ir
3
Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
LEAD_AUTHOR
Aguiar C, Almeida A, Araujo P, Vasconcelos GS, Chaves EMC, Do vale OC.2012. Anticonvulsant effects of agomelatine in mice. Epilepsy Behav, 24: 324-328.
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Asadi-Pooya A, Nikseresht A ,Yaghoubi E. 2012 Old Remedies for Epilepsy: Avicenna’s Medicine. Iran Red Crescent Med J, 14: 174-177.
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Borowicz K.K, Luszczki J, Kleinrok Z, Czuczwar S.J. 2000. 7-Nitroindazole, a nitric oxide synthase inhibitor, enhances the anticonvulsive action of ethosuximide and clonazepamagainst pentylenetetrazol-induced convulsions, J Neural Transm, 101: 1117-1126.
4
Castel-Branco M, Alves G, Figueiredo I, Falcao A, Caramona MM. 2009. The maximal electroshock seizure (MES) model in the preclinical assessment of potential new antiepileptic drugs. Methods Find Exp Clin Pharmacol, 31: 101-106.
5
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Engelborghs S, D’Hooge R , De Deyn P. 2000. Pathophysiology of epilepsy. Acta Neurol Belg, 100: 201-213.
8
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ORIGINAL_ARTICLE
Effect of different brewing times on antioxidant activity and polyphenol content of loosely packed and bagged black teas (Camellia sinensisL.)
Objective: Determination and comparison of the effect of infusion time on the antioxidant activity and total polyphenol contents of bagged and loosely packed black teas. Materials and Methods: For twenty loosely packed and eleven bagged tea samples, the antioxidant activity and total polyphenol content were analyzed using FRAP and Folin-Ciocalteau methods, respectively. The ANOVA with Tukey post-hoc test and independent t-test were used for statistical analysis. Results: The antioxidant activity and polyphenol content of various brands of tea samples were significantly different. There were significant differences in the antioxidant activity of loosely packed teas between 5, 15(p=0.03), 30(p=0.02) and 60(p=0.007) minutes of brewing times. Besides, there was a significant difference in antioxidant activity of bagged samples infused for 1 minute with four other infusion time points (p<0.001). In the case of polyphenol content, in loosely-packed tea samples, there were not significant differences between different brewing times (p=0.15). However, in bagged samples, the polyphenol contents of samples that were brewed for 1 minute were significantly lower than samples brewed for 3, 4, and 5 minutes (p<0.05). The antioxidant activity and polyphenol content of tea bags were significantly higher than those ofloosely-packed forms of the same brands at 5-min of brewing time (p<0.001). Conclusion: The infusion time and the form of tea (loosely packed or bagged) were shown to be important determinants of the antioxidant activity and polyphenol content of black tea infusions in addition to the variety, growing environment and manufacturing conditions.
https://ajp.mums.ac.ir/article_5914_3d176e56b627d07159c6ae3201331d89.pdf
2016-05-01
313
321
10.22038/ajp.2016.5914
Antioxidant
polyphenol
Tea
Infusion time
Zeinab
Nikniaz
znikniaz@hotmail.com
1
Liver and Gastrointestinal disease research center, Tabriz University of medical sciences, Tabriz-Iran
AUTHOR
Reza
Mahdavi
znikniaz@gmail.com
2
Nutrition research center, Tabriz University of medical sciences
LEAD_AUTHOR
Seyed Jamal
Ghaemmaghami
sjghaemmaghami@gmail.com
3
Nutrition Research Centre, Tabriz University of medical sciences, Tabriz-Iran
AUTHOR
Neda
Lotfi Yaghin
neda_lotfi@hotmail.com
4
Student Research Committee, Tabriz University of medical sciences
AUTHOR
Leila
Nikniaz
nikniaz_l@gmail.com
5
Tabriz Health Services Management Research Center, Tabriz University of Medical Sciences, Tabriz-Iran
AUTHOR
Anesini C, Ferraro GE, Filip R. 2008. Total Polyphenol Content and Antioxidant Capacity of Commercially Available Tea (Camellia sinensis) in Argentina. J Agr Food Chem, 56: 9225–9229.
1
Armoskaite V, Ramanauskiene K, Maruska A, Razukas A, Dagilyte A, Baranauskas A, Briedis V. 2011. The analysis of quality and antioxidant activity of green tea extracts. J Med Plants Res, 5: 811-816.
2
Astill C, Birch MR, Dacombe C, Humphrey PG, Martin PT. 2001. Factors Affecting the Caffeine and Polyphenol Contents of Black and Green Tea Infusions. J Agr Food Chem, 49: 5340−5347.
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Benzie IFF, Strain JJ. 1996. The Ferric Reducing Ability of Plasma (FRAP) as a measure of ‘‘Antioxidant Power’’: The FRAP Assay, Anal Biochem, 239: 70-76.
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Kyle JA, Morrice PC, McNeill G, Duthie GG. 2007. Effects of infusion time and addition of milk on content and absorption of polyphenol from black tea. J Agri Food Chem, 13:55: 4889-94.
15
Lachman J, Hosnedl V, Pivec V, Orsak M. 2003. Polyphenol content in green, black and oolong tea (Camellia sinensis/L./kuntze) infusions in different times of tea maceration. Scientia Agriculturae Bohemica, 34: 22–28.
16
Mahdavi R, Nikniaz Z, Rafraf M, Jouyban A. 2011. Determination and comparison of the total polyphenol contents of fresh and commercial fruit juices. BFJ, 113: 6, 744-752.
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18
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19
Rusak G, Komes D, Likic S, Horz ˇic D, Kovac M. 2008. Phenolic content and antioxidative capacity of green and white tea extracts depending on extraction conditions and the solvent used. Food Chem, 110: 852–858.
20
Ryan L, PetitLisa Ryan S, Petit S. 2010. Addition of whole, semiskimmed, and skimmed bovine milk reduces the total antioxidant capacity of black tea. Nutr Res, 30: 14–20.
21
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22
Sarkar A, Bhaduri A. 2001. Black tea is a powerful chemopreventor of reactive oxygen and nitrogen species: comparison with its individual catechin constituents and green tea.Bio chem. Bio phy Res, 284: 173-178.
23
Scalbert A, Johnson IT, Saltmarsh M. 2005. Polyphenols: Antioxidants and beyond. Am J ClinNutr, 81(suppl): 215S-7S.
24
Singleton VL, Joseph A, Rossi, JRJA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic, 16:144-158.
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Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999.Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin Ciocalteu reagent. Methods Enzymol, 299: 152 178.
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Tsao R. and Yang R. 2003. Optimization of a new mobile phase to know the complex and real polyphenolic composition: towards a total phenolic index using high-performance liquid chromatography. J chromatgr, 1018: 29-40.
27
Yashin A, Yashin Y, Nemzer B. 2011. Determination of Antioxidant Activity in Tea Extracts, and Their Total Antioxidant Content. Am J Biomed Sci, 3: 322-335.
28
Yao L, Liu X, Jiang Y, Caffin N, Arcy BD, Singanusong R, Datta N, Xu Y. 2006. Compositional analysis of teas from Australian supermarkets. Food Chem, 94: 115–122.
29
ORIGINAL_ARTICLE
Evaluation of antidepressant-like effects of aqueous and ethanolic extracts of Pimpinella anisum fruit in mice
Objective: Pimpinella anisum (P. anisum) has different pharmacological properties such as anticonvulsant, analgesic, tranquilizer, antidepressant and anti-anxiety effects. In this study the antidepressant-like effect of aqueous and ethanolic extracts of P. anisum fruit in mice was investigated.
Materials and Methods: Forced swimming test (FST) and tail suspension test (TST) were used to determine the antidepressant effects of P. anisum (50, 100 and 200 mg/kg, i.p.) fruit extracts. Fluoxetine (20 mg/kg, i.p.) and imipramine (30 mg/kg, i.p.) were used as standard drugs.
Results: All the three doses of aqueous and ethanolic extracts (except 50 mg/kg of aqueous extract in FST) significantly and dose-dependently reduced the immobility times in both FST and TST. All doses of extracts increased the swimming time dose-dependently, without any significant change in climbing time. In addition, all doses of ethanolic extract reduced immobility times and increased swimming time insignificantly higher than aqueous extract. But, the two extracts decreased the duration of climbing time similarly. Fluoxetine and imipramine decreased immobility time in both tests. Fluoxetine increased the swimming time without modifying climbing time. In contrast, imipramine increased climbing time without any significant change in swimming time.
Conclusion: The results of this study suggest that P. anisum possesses an antidepressant-like activity similar to that of fluoxetine, which has a potential clinical value for application in the management of depression.
https://ajp.mums.ac.ir/article_5915_5c7e809dbb28e877abce3305b40bfd0b.pdf
2016-05-01
322
328
10.22038/ajp.2016.5915
Pimpinella anisum
Forced swimming test
Tail suspension test
Antidepressant activity
Mice
Zahra
Shahamat
zahra_shahamat@yahoo.com
1
Depatment of Pharmacology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
AUTHOR
Saeid
Abbasi-Maleki
dr.s.a.maleki@gmail.com
2
Department of Pharmacology, Urmia Branch, Islamic Azad University Urmia, Iran
LEAD_AUTHOR
Saeid
Mohammadi Motamed
mohamadimotamed.s@iaups.ac.ir
3
Department of Pharmacognosy, Pharmaceutical Science Branch, Islamic Azad University, Tehran, Iran
AUTHOR
De Sousa DP.2011. Analgesic-like activity of essential oils constituents. Mol ,16: 2233-2252.
1
Detke MJ, Rickels M, Lucki I. 1995. Active behaviors in the rat forced swimming test differentially produced by serotonergic and noradrenergic antidepressants. Psychopharmacol, 121: 66-72.
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3
EL-Hodairy FA. 2014. Neuroprotective effects of pimpinella anisum on neurotoxicity induced by bisphinol a on normal and diabetic rats. Int J Pharm
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Pharm Sci, 6; 3: 9-12.
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Emamghoreishi M,Talebianpour MS. 2009. Antidepressant effect of Melissa officinalis in forced swim test. DARU, 17: 42-47.
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Heidari MR, Ayeli M.2005. Effects of methyl alcoholic extract of Pimpinella anisum L. on picrotoxin induced seizure in mice and its probable mechanism. Sci J kurdestan Univ of MedSic(SJKU), 10:1-8.
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11
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12
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13
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Rodrigues VM, Rosa PTV, Marques MOM, Petenate AJ, Meireles MAA. 2003. Supercritical extraction of essential oil from aniseed (Pimpinella anisum L) using CO2: solubility, kinetics, and composition data. J Agricul Food Chem, 51: 1518-1523.
16
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Scapagnini G, Davinelli S, Drago F, De Lorenzo A, Oriani G.2012. Antioxidants as antidepressants: fact or fiction?. CNS Drugs, 26:477-490.
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23
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24
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25
ORIGINAL_ARTICLE
Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity
Objective: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. Materials and Methods: HepG2 were incubated with irinotecan (100 µM), heat-killed cells (0.025 µg/ml) + irinotecan (100 µM), and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum A7 + irinotecan (100 µM). Phosphate buffered saline (PBS) was used as negative control. Results: Irinotecan was shown to induce DNA damage in HepG2 cells. The results showed that heat-killed cells (0.025 µg/ml) and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum significantly reduce irinotecan- induced DNA damage. Conclusion: Our results indicate that L. plantarum A7 can decrease the genotoxic effects of irinotecan in HepG2 cells, in vitro. This finding may be supportive for the optimization of therapeutic efficacy in irinotecan treatment.
https://ajp.mums.ac.ir/article_5962_f3d10d3d831ba187f1f82268f4e3a7fd.pdf
2016-05-01
329
335
10.22038/ajp.2016.5962
Irinotecan
Lactobacillus plantarum A7
Anti-genotoxicity
HepG2
Comet assay
Soheila
Sepahi
sepahi66@gmail.com
1
Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
AUTHOR
Abbas
Jafarian-Dehkordi
jafarian@pharm.mui.ac.ir
2
Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
AUTHOR
Maryam
Mirlohi
mirlohi@mui.ac.ir
3
Food Security Research Centre, School of Nutrition and Food Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
AUTHOR
kobra
shirani
shiranik911@mums.ac.ir
4
Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of
Medical Sciences, Mashhad, Iran.
AUTHOR
Mahmoud
Etebari
etebari@pharm.mui.ac.ir
5
Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
LEAD_AUTHOR
Apás AL, González SN, Arena ME. 2014. Potential of goat probiotic to bind mutagens. Anaerobe, 28: 8-12
1
Burns AJ, Rowland IR. 2004. Antigenotoxicity of probiotics and prebiotics on faecal water-induced DNA damage in human colon adenocarcinoma cells. Mutat Res, 551: 233-43.
2
Cai Z, Yang J, Shu X and Xiong X. 2014. Chemotherapy-associated hepatotoxicity in colorectal cancer. Vascular, 14: 15.
3
Caldini G, Trotta F, Villarini M, Moretti M, Pasquini R, Scassellati-Sforzolini G, Cenci G. 2005. Screening of potential lactobacilli antigenotoxicity by microbial and mammalian cell-based tests. Int J Food Microbiol, 102: 37-47.
4
Chávez-Tapia NC, González-Rodríguez m L, Jeong M, López-Ramírez Y, Barbero-Becerra V, Juárez-Hernández E, Romero-Flores J. L, Arrese M, Méndez-Sánchez N and Uribe M. 2015. Current evidence on the use of probiotics in liver diseases. J Funct Foods, 17:137-151.
5
Cragg GM, Newman DJ. 2005. Plants as a source of anti-cancer agents. J Ethnopharmacol, 100: 72-79.
6
Etebari M, Ghannadi A, Jafarian-Dehkordi A, Ahmadi F. 2012. Genotoxicity evaluation of aqueous extracts of co-toneaster discolor and Alhagi pseudalhagi by com-et assay. J Res Med Sci, 17:S237–S241.
7
Fazeli H, Moshtaghian J, Mirlohi M, Shirzad M. 2010. Reduction in serum lipid parameters by incorporation of a native strain of Lactobacillus Plantarum A7 in Mice. J Diabetes Metab Disord, 9: 1-7.
8
Jafarian A, Zolfaghari B, Shirani K. 2014. Cytotoxicity of different extracts of arial parts of Ziziphus spina-christi on Hela and MDA-MB-468 tumor cells. Adv Biomed Res, 3: 38.
9
Kahouli I, Tomaro-Duchesneau C, Prakash S. 2013. Probiotics in colorectal cancer (CRC) with emphasis on mechanisms of action and current perspectives. J Med Microbiol, 62: 1107-23.
10
Kontek R, Drozda R, Śliwiński M, Grzegorczyk K. 2010. Genotoxicity of irinotecan and its modulation by vitamins A, C and E in human lymphocytes from healthy individuals and cancer patients. Toxicol In Vitro, 24: 417-24.
11
Kumar K, Sastry N, Polaki H, Mishra V. 2015. Colon Cancer Prevention through Probiotics: An Overview. J Cancer Sci Ther, 7: 081-92.
12
Lévesque É, Bélanger A-S, Harvey M,Couture F, Jonker D, Innocenti F, Cecchin E, Toffoli G and Guillemette C. 2013. Refining the UGT1A haplotype associated with irinotecan-induced hematological toxicity in metastatic colorectal cancer patients treated with 5-fluorouracil/irinotecan-based regimens. J Pharmacol Exp Ther, 345: 95-101.
13
Parvez S, Malik K, Ah Kang S, Kim HY. 2006. Probiotics and their fermented food products are beneficial for health. J Appl Microbiol, 100: 1171-85.
14
Pool‐Zobel BL, Münzner R, Holzapfel WH. 1993. Antigenotoxic properties of lactic acid bacteria in the S. typhimurium mutagenicity assay. Nutr Cancer, 20: 261–270.
15
Raman M, Ambalam P, Kondepudi KK, Pithva S, Kothari C, Patel AT, Purama RK, Dave JM, Vyas BR. 2013. Potential of probiotics, prebiotics and synbiotics for management of colorectal cancer. Gut microbes, 4: 181-92.
16
Razavi-Azarkhiavi K, Behravan J, Mosaffa F, Sehatbakhsh S, Shirani K, Karimi G. 2014. Protective effects of aqueous and ethanol extracts of rosemary on H2O2-induced oxidative DNA damage in human lymphocytes by comet assay. J Complement Integr Med, 11: 27-33.
17
Sadeghi-Aliabadi H, Mohammadi F, Fazeli H, Mirlohi M. 2014. Effects of Lactobacillus plantarum A7 with probiotic potential on colon cancer and normal cells proliferation in comparison with a commercial strain. Iran J Basic Med Sci, 17: 815–819.
18
Santos A, Zanetta S, Cresteil T, Deroussent A, Pein F, Raymond E, Vernillet L, Risse ML, Boige V, Gouyette A, Vassal G. 2000. Metabolism of irinotecan (CPT-11) by CYP3A4 and CYP3A5 in humans. Clin Cancer Res, 6: 2012-20.
19
Vajro P, Mandato C, Licenziati MR, Franzese A, Vitale DF, Lenta S, Caropreso M, Vallone G, Meli R. 2011. 2011. Effects of Lactobacillus rhamnosus strain GG in pediatric obesityrelated liver disease. J Pediatr Gastroenterol Nutr, 52: 740–743.
20
ORIGINAL_ARTICLE
Evaluation of antibacterial effects of Zataria multiflora Boiss extracts against ESBL-producing Klebsiella pneumoniae strains
Objective: There are few therapeutic options for treatment of multidrug resistant Klebsiella pneumoniae isolates as a hospital infectious agent (nosocomial infection). The aim of this study was to evaluate the antibacterial activity of Zataria multiflora Boiss extracts against ESBL-producing Klebsiella pneumoniae strains. Materials and Methods: This study was conducted on 100 K. pneumoniae isolates from two hospitals in Tehran, Iran. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion and microdilution broth methods and detection of ESBL was carried out according to CLSI guidelines. The blaCTX-M-15plasmid genewas detected by PCR and sequencing methods. Extracts susceptibility test was performed by broth microdilution method. Results: Among 100 K. pneumoniae strains, 48 (48%) were ESBL positive. In this study, fosfomycin, colistin and tigecycline were more active than other antibiotics. The existence of blaCTX-M-15 was detected in 30 (62.5%) of 48 ESBL-producing isolates. The chloroformic extract showed potent activity against ESBL-producing K. pneumoniae strains (MIC50 = 1.56 mg/ml and MIC90=3.12mg/ml). The MIC50 and MIC90 (The MIC50 represents the MIC value at which ≥50% of the isolates in a test population are inhibited and the MIC90 represents the MIC value at which ≥90% of the strains within a test population are inhibited) were 3.12 and 6.25 mg/ml and 6.25 and 12.5 mg/ml for methanolic and acetonic extracts, respectively. Conclusions: The incidence of ESBL-producing K. pneumoniae is very high. Therefore, detection of ESBL-producing K. pneumoniae isolates is of great importance in identifying drug resistance patterns in K. pneumoniae isolates and in control of infections. Zataria multiflora may have the potential to be used against multidrug resistant organisms such as clinical isolates of ESBL-producing K. pneumoniae.
https://ajp.mums.ac.ir/article_6138_558d599e3f51beae1499dfb0e201206c.pdf
2016-05-01
336
343
10.22038/ajp.2016.6138
Klebsiella pneumoniae
Extended-Spectrum-β-Lactamases (ESBLs)
Zataria multiflora
Antibiotic resistance
Masoud
Dadashi
m_d6512@yahoo.com
1
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Ali
Hashemi
hashemi1388@yahoo.com
2
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
Gita
Eslami
g_eslami@yahoo.com
3
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Fatemeh
Fallah
dr_fallah@yahoo.com
4
Pediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Hossein
Goudarzi
farassooali@yahoo.com
5
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Soroor
Erfanimanesh
s_erfanimanesh@yahoo.com
6
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Arezou
Taherpour
are_taherpour@yahoo.com
7
Department of Microbiology, Kurdistan University of Medical Sciences, Sanandaj, Iran
AUTHOR
Al Akeel R, Al-Sheikh Y, Mateen A, Syed R, Janardhan K, Gupta VC. 2014. Evaluation of antibacterial activity of crude protein extracts from seeds of six different medical plants against standard bacterial strains. Saudi J Biol Sci. 21:147-151.
1
Betoni JE, Mantovani RP, Barbosa LN, Di Stasi LC, Fernandes Junior A. 2006. Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases. Mem Inst Oswaldo Cruz, 101: 387-390.
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Bradford PA. 2001. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev, 14: 933-951
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Cowan MM. 1999. Plant products as antimicrobial agents. Clin Microbiol Rev. 12: 564-582.
5
Devatkal SK, Jaiswal P, Jha SN, Bharadwaj R, Viswas KN. 2013. Antibacterial activity of aqueous extract of pomegranate peel against Pseudomonas stutzeri isolated from poultry meat. J Food Sci Technol, 50: 555-560.
6
Eftekhar F ZS, Yusefzadi M, Hadian J, Ebrahimi SN. 2011. Antibacterial activity of Zataria multiflora Boiss essential oil against extended spectrum β lactamase produced by urinary isolates of Klebsiella pneumoniae. Jundishapur J Microbiol, 4: S43-S9.
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Mahboubi M, Bidgoli FG. 2010. Antistaphylococcal activity of Zataria multiflora essential oil and its synergy with vancomycin. Phytomedicine, 17: 548-550.
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14
Motevasel M OM, Zomorodian K, Farshad S. 2013. A Study of the Effect of Zataria multiflora Extract on Methicillin Resistant Staphylococcus aureus. Jundishapur J Microbiol, 6: 5453.
15
Nukaga M, Mayama K, Hujer AM, Bonomo RA, Knox JR. 2003. Ultrahigh resolution structure of a class A beta-lactamase: on the mechanism and specificity of the extended-spectrum SHV-2 enzyme. J Mol Biol, 328: 289-301.
16
Orencia MC, Yoon JS, Ness JE, Stemmer WP, Stevens RC. 2001. Predicting the emergence of antibiotic resistance by directed evolution and structural analysis. Nat Struct, 8: 238-242.
17
Pagani L, Perilli M, Migliavacca R, Luzzaro F, Amicosante G. 2000. Extended-spectrum TEM- and SHV-type beta-lactamase-producing Klebsiella pneumoniae strains causing outbreaks in intensive care units in Italy. Eur J Clin Microbiol, 19: 765-772.
18
Saei-Dehkordi SS, Tajik H, Moradi M, Khalighi-Sigaroodi F. 2010. Chemical composition of essential oils in Zataria multiflora Boiss. from different parts of Iran and their radical scavenging and antimicrobial activity. Food Chem Toxicol, 48: 1562-1567.
19
Salarbashi D, Tajik S, Shojaee-Aliabadi S, Ghasemlou M, Moayyed H, Khaksar R Noghabi MS. 2014. Development of new active packaging film made from a soluble soybean polysaccharide incorporated Zataria multiflora Boiss and Mentha pulegium essential oils. Food Chem, 146: 614-622.
20
Sirot D, Sirot J, Labia R, Morand A, Courvalin P, Darfeuille-Michaud A, Perroux R, Cluzel R. 1987. Transferable resistance to third-generation cephalosporins in clinical isolates of Klebsiella pneumoniae: identification of CTX-M-1, a novel beta-lactamase. J Antimicrob Chemother, 20: 323-334.
21
Shin J, Kim DH, Ko KS. 2011. Comparison of CTX-M-14- and CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae isolates from patients with bacteremia. J Infect, 63: 39-47.
22
Zomorodian K, Saharkhiz MJ, Rahimi MJ, Bandegi A, Shekarkhar G, Bandegani A, Pakshir K, Bazargani A. 2011. Chemical composition and antimicrobial activities of the essential oils from three ecotypes of Zataria multiflora. Phcog Mag, 7: 53-59.
23
ORIGINAL_ARTICLE
Evaluation of anxiolytic-like activity of Vitis vinifera juice in mice
Objective: Scientificstudies have shown that Vitis vinifera (V. vinifera) contains flavonoids and stillbenoids. Flavonoids are well known to possess anxiolytic activities. In view of the idea that flavonoids present in V. vinifera could be useful in anxiety, we evaluated anxiolytic-like activity of V. vinifera juice (VVJ). Materials and Methods: Light/dark box and the open field test were used to assess the anxiolytic potential of V. vinifera juice (VVJ). The juice was given orally by gavage at the dose of 4 and 8 mL/kg body weight. Diazepam (1 mg/kg i.p.) was used as the standard drug. Results: It was observed that the juice produced significant and dose dependent increase in the time spent in light cubicle (p<0.001), transfer latency from the light to dark cubicle (p<0.001) and the number of transitions between the two cubicles (p<0.001) as compared with the control group. V. vinifera also demonstrated significant and dose dependent increase in ambulation (P<0.001) and rearing (p<0.001) in open field test as compared to the control group. Conclusion: In conclusion, the present study establishes the anxiolytic-like activity of VVJ in animal models of anxiety.
https://ajp.mums.ac.ir/article_5818_88b40c7551236d88a3c4373f17972e4d.pdf
2016-05-01
344
350
10.22038/ajp.2016.5818
Vitis vinifera
Anxiety
Flavonoids
Open field test
The light/dark box
Muhammad
Aslam
pharmacologist1@yahoo.com
1
Department of Pharmacology, Faculty of Pharmacy, University of Karachi-75270, Pakistan
AUTHOR
Nuzhat
Sultana
nuztsultana@gmail.com
2
Department of Pharmacology, Faculty of Pharmacy, University of Karachi-75270, Pakistan
LEAD_AUTHOR
Alan JG. 2000. Psychiatric and Somatic Markers of Anxiety: Identification and Pharmacologic Treatment. Prim Care Comp J Clin Psychiatry, 2: 49–54.
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31
ORIGINAL_ARTICLE
Safety assessment of rice bran oil in a chicken embryo model
Objective: Rice Bran Oil (RBO) is extracted from the outer layer of rice. Little information is available regarding its safety. The present study was conducted to assess its safety in chicken embryo model. Materials and Methods: RBO was injected on day 4 of incubation of chickens. The tissues and serum samples were collected. Oxidative stress parameters in the liver, kidney and brain and biochemical parameters of serum were measured. The deformities were also investigated. Results: The changes in the liver enzymes activity were not statistically significant. There was significant decrease and increase in lipid peroxidation and glutathione level, respectively. It is suggested that RBO is a natural antioxidant source. Low-density lipoprotein cholesterol (LDL) also decreased. No abnormal findings were observed in the chickens. Conclusion: No toxic effect was observed following RBO administration in chicken embryos. This study showed that RBO is not a safety concern.
https://ajp.mums.ac.ir/article_5618_b4e6ea4986ccc012598e3fd4be1dbd2d.pdf
2016-05-01
351
356
10.22038/ajp.2016.5618
Safety assessment
Rice bran oil
Malformations
Oxidative stress
Atefeh
Araghi
araghi1360@gmail.com
1
Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
AUTHOR
Saeed
Seifi
seifi@yahoo.com
2
Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
AUTHOR
Reza
Sayrafi
r.seyrafi@gmail.com
3
Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
AUTHOR
Parisa
Sadighara
sadighara@farabi.tums.ac.ir
4
Department of Environmental Health Engineer, Food Safety Division, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
Alvin B, Rein D, Schäfer A, Monnard I, Gremaud G, Lambelet P, Bertoli C. 2005.Similar cholesterol–lowering properties of rice bran oil, with varied γ–oryzanol, in mildly hypercholesterolemic men. Eur J Nutr, 44: 163-173.
1
Chotimarkorn,C, Benjakul S, Silalai N. 2008. Antioxidant components and properties of five long grained rice bran extracts from commercial available cultivars in Thailand. Food Chem, 111: 636-641.
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4
LiangY, Yu G, Qinlu L, Feijun L, Wei W, Qian L, Ying L.2014. A review of the research progress on the bioactive ingredients and physiological activities of rice bran oil. Eur Food Res Technol, 238: 169-176.
5
Liu WX, Jia FL, He YY, Zhang BX.2012. Protective effects of 5-methoxypsoralen against acetaminophen- induced hepatotoxicity in mice. World J Gastroenterol, 18:2197-2202
6
Hagl S, Alexa K, Christina S, Schamim H, Ion C, Marc B, Hesham El. 2013.Rice bran extract protects from mitochondrial dysfunction in guinea pig brains. Pharmacol Res, 76: 17-27.
7
Morales G, Paredes A.2014. Antioxidant activities of Lampaya medicinalis extracts and their main chemical constituents. Complement Altern Med, 14:259-271.
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Ozer J, Ratner M, Shaw M, Bailey W. Schomaker S.2008. The current state of serum biomarkers of hepatotoxicity. Toxicology, 245: 194-205.
9
Pourmirza AA. 2000.Toxic Effects of Malathion and Endosulfan on Chick Embryo. J. Agr. Sci. Tech, 2:161-166.
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Posuwan J, Pattaneeya P, Vijittra L, Uruwan Y, Ruethaithip S, Rin C, Ratchanee K. 2013. Long-term supplementation of high pigmented rice bran oil on amelioration of oxidative stress and histological changes in streptozotocin-induced diabetic rats fed a high fat diet; Riceberry bran oil. Food chem, 138:501-508.
11
Shih C, Chia-Jung H, Sing-Chung L, Shwu-Huey Y, Wen-Chi H, Hsing-Hsien C. 2011. Preventive effects of rice bran oil on 1, 2-dimethylhydrazine/dextran sodium sulphate-induced colon carcinogenesis in rats. Food Chem, 126:562-567.
12
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Surai PF. 1999.Tissue specific changes in the activities of antioxidant enzymes during the development of the chicken embryo. Br Poult Sci, 40:397-405.
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16
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17
ORIGINAL_ARTICLE
Chemical composition and antibacterial properties of essential oil and fatty acids of different parts of Ligularia persica Boiss
Objectives: The objective of this research was to investigate the chemical composition and antibacterial activities of the fatty acids and essential oil from various parts of Ligularia persica Boiss (L. persica) growing wild in north of Iran. Materials and Methods: Essential oils were extracted by using Clevenger-type apparatus. Antibacterial activity was tested on two Gram-positive and two Gram-negative bacteria by using micro dilution method. Results: GC and GC∕MS analysis of the oils resulted in detection of 94%, 96%, 93%, 99% of the total essential oil of flowers, stems, roots and leaves, respectively. The main components of flowers oil were cis-ocimene (15.4%), β-myrcene (4.4%), β-ocimene (3.9%), and γ-terpinene (5.0%). The major constituents of stems oil were β-phellandrene (5.4%), β-cymene (7.0%), valencene (3.9%). The main compounds of root oil were fukinanolid (17.0%), α-phellandrene (11.5%) and Β-selinene (5.0%) and in the case of leaves oil were cis-ocimene (4.8%), β-ocimene (4.9%), and linolenic acid methyl ester (4.7%). An analysis by GC-FID and GC-MS on the fatty-acid composition of the different parts of L. persica showed that major components were linoleic acid (11.3-31.6%), linolenic acid (4.7-21.8%) and palmitic acid (7.2-23.2%). Saturated fatty acids were found in lower amounts than unsaturated ones. The least minimum inhibition concentration (MIC) of the L. persica was 7.16 μg/ml against Pseudomonas aeruginosa. Conclusion: Our study indicated that the essential oil from L. persica stems and flowers showed high inhibitory effect on the Gram negative bacteria. The results also showed that fatty acids from the stems and leaves contained a high amount of poly-unsaturated fatty acids (PUFAs).
https://ajp.mums.ac.ir/article_5722_795a92102648ad2432a2b3d3f051d4ee.pdf
2016-05-01
357
365
10.22038/ajp.2016.5722
Ligularia persica
Asteraceae
Essential oil composition
Fatty Acids
Antibacterial
Maryam
Mohadjerani
m.mohajerani@umz.ac.ir
1
Department of Molecular and Cell Biology, Faculty of basic Sciences, University of Mazandaran, Babolsar, Iran
LEAD_AUTHOR
Rahman
Hosseinzadeh
r.hosseinzadeh@umz.ac.ir
2
Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran
AUTHOR
Maryam
Hosseini
maryam.hs6927@gmail.com
3
Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran
AUTHOR
Adams RP. 2007. Identification of essential oil components by gas chromatography mass spectroscopy. 4th edition, pp. 803, Allured publishing corporation, Carol Stream, IL, USA.
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