ORIGINAL_ARTICLE
Otostegia persica (Lamiaceae): A review on its ethnopharmacology, phytochemistry, and pharmacology
Abstract Objective: The current study summarizes the updated information concerning the ethnopharmacology, Phytochemistry, and pharmacology of Otostegia persica Boiss. (Lamiaceae), an endemic medicinal plant in south and southeast of Iran. Materials and Methods: Information was collected through bibliographic investigation from scientific journals, books, theses, reports, and electronic search (databases SCOPUS, Google Scholar, Web of Science, and Science Direct). Moreover, documentation from unpublished resources and ethnobotanical surveys has been used. The present review covers the literature available from 2003 to 2013. Results: In traditional systems of medicine, this plant is reputed for treating diabetes, arthritis, gastric discomfort, headache, rheumatism, sedative activities, regulating blood pressure, and hyperlipidemia. Phytochemical screening of active components and mineral element evaluation of this species have been reported. Several types of diterpenoids and flavonols including morin, kaempferol, and quercetin are identified from the plant. Most of the pharmacological activity of this plant resides in its flavonoid fraction which causes antimicrobial and antioxidant activities. Various pharmacological studies on O. persica show antimicrobial, antioxidant, anti-inflammatory, anti-diabetic, anti-aphid, and hepatoprotective activities. Conclusion: Being an endemic plant of Iran, this species is an important medicinal herb which can be used for various purposes. This review might be helpful for scientists and researchers to find new chemical entities responsible for its claimed traditional uses and discover new lead compounds for diseases mentioned.
https://ajp.mums.ac.ir/article_2161_9e5779fd4a4ab6f8eb80501572f77ac9.pdf
2014-03-01
79
88
10.22038/ajp.2014.2161
Otostegia
Pharmacology
Phytochemistry
Toxicity
Therapeutics
Traditional Medicine
Zahra
Sadeghi
sadeghi.phytochem@gmail.com
1
Agricultural Research Center, High Educational complex of Saravan, I. R. Iran
AUTHOR
Maryam
Akaberi
akaberim911@mums.ac.ir
2
Biotechnology Research Center and School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
LEAD_AUTHOR
Jafar
Valizadeh
walisade@hamoon.usb.ac.ir
3
Department of Biology, University of Sistan & Baluchestan, Zahedan, I. R. Iran
AUTHOR
Adewole SO, Caxton-Martins EA, Ojewole JAO. 2007. Protective Effect of Quercetin on the Morphology of Pancreatic β-Cells of Streptozotocin-Treated Diabetic Rats. Afr J Tradit Complement Altern Med, 4: 64-74.
1
Akbarzadeh S, Bazzi P, Daneshi A, Nabipour I, Pourkhalili Kh, Mohebbi Gh, Sartavi K, Abdi M, Mirzaei M, Bargahi A. 2012. Anti-diabetic effect of Otostegia persica extract on diabetic rats. J Med Plant Res, 6: 3176-3180.
2
Asghari G, Nourallahi H, Havaie SA, Issa L. 2006. Antimicrobial activity of Otostegia persica Boiss. extracts. Res Pharm Sci, 1: 53-58.
3
Ayatollahi SAM, Kobarfard F, Asgarpanah J, Rahmati Roodsari M, Fanai Gh, Choudhary MI. 2009. Diterpenoids of Otostegia persica (Burm) Boiss. DARU, 174: 290-293.
4
Ayatollahi SAM, Kobarfard F, Asgarpanah J. 2007. Chemical Constituents from Otostegia persica,. J Chem Soc Pakistan, 291: 102.
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Ayatollahi SAM, Kobarfard F, Asgarpanah J, Choudhary MI. 2010. Antiglycation Activity of Otostegia persica (Burm.) Boiss. Afr J Biotech, 924: 3645-3648.
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Bezenjani SN, Pouraboli I, Malekpour Afshar R, Mohammadi Gh. 2012. Hepatoprotective Effect of Otostegia persica Boiss. Shoot Extract on Carbon Tetrachloride-Induced Acute Liver Damage in Rats. Iran J Pharm Res, 11: 1235-1241.
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Ganjali A, Sotoudeh A, Jahanshahi A, Ashrafzadeh TakhtfooladiM, Bazzazan, A, RoodbariN, Pourramezani Harati M. 2013. Otostegia persica extraction on healing process of burn wounds. Acta Cirurgica Brasileira, 28: 407.
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21
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28
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30
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31
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33
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34
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37
ORIGINAL_ARTICLE
Identification of phenolic antioxidants in Ipomoea mauritiana jacq. using spectrophotometric and mass spectroscopic studies
Objective: Ipomoea mauritiana is used in both Ayurveda and folk medicine systems. The tuberous roots are known to be diuretic, depurative, carminative, and anthelmintic. The objective of the current study was to identify phenolic antioxidants from I. mauritiana using spectrophotometric and LC-MS analysis. Materials and Methods: An activity-guided fractionation and purification process was used to identify the antioxidative components from I. mauritiana tuber. Dried mature tubers of I. mauritiana were extracted with 80% methanol and then partitioned by chloroform, ethyl acetate, acetone, and methanol. The acetone fraction showed the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity among four fractions and was subjected to separation and purification using preparative reverse-phase high-performance liquid chromatography (HPLC). Results: Two compounds were separated from the acetone fraction using preparative LC fraction collector. The purified compounds were screened for their antioxidative potential using DPPH assay. The compounds were subjected to LC-MS analysis in ESI negative mode. One of the compounds was identified as Caffeoyl glucose based on the mass fragmentation. Conclusion: The acetone fraction showed highest radical scavenging activity and the phytoconstituents of the same were identified by LC-MS/MS analysis.
https://ajp.mums.ac.ir/article_1104_d2642c095162fe73fde6fc3d0407a676.pdf
2014-03-01
89
96
10.22038/ajp.2014.1104
Ipomoea mauritiana
Phenolics
DPPH
LC-ESI-MS
Cheruthazhakkat
Sulaiman
slmnct@gmail.com
1
Centre for Medicinal Plants Research, Arya Vaidya Sala Kottakkal-676503, Kerala, India
LEAD_AUTHOR
Sivadasan Pillai
Geetha
2
Centre for Medicinal Plants Research, Arya Vaidya Sala Kottakkal-676503, Kerala, India
AUTHOR
Balachandran
Indira
3
Centre for Medicinal Plants Research, Arya Vaidya Sala Kottakkal-676503, Kerala, India
AUTHOR
Anttonen MJ, Karjalainen RO. 2006. High-performance liquid chromatography analysis of black currant (Ribes nigrum L.) fruit phenolics grown either conventionally or organically. J Agric Food Chem, 54: 7530-7538.
1
Apea-Bah FB, Hanafi M, Dewi RT, Fajriah S, Darmawan A, Artanti N, Lotulung P, Ngadymang P, and Minarti B. 2009. Assessment of the DPPH and α-glucosidase inhibitory potential of gambier and qualitative identification of major bioactive compound. J Med Plants Res, 3: 736-757.
2
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Sivarajan VV and Balachandran I. 1994. Ayurvedic Drugs and Their Plant Sources, Oxford and IBH Publishing, New Delhi, India, pp. 510-512
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Tepe B, Daferera D, Sokmen A, Sokmen M and Polissiou M. 2005. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food Chem, 90: 333-340.
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Warrier PK, Nambiar VPK. Ramankutty C. 1995 Indian Medicinal Plants: A Compendium of 500 Species, Orient Longman, Chennai, India Vol. 3. pp. 222-228.
25
Zheng W and Wang SY. 2001. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem, 49: 5165-5170.
26
ORIGINAL_ARTICLE
Investigation of total phenolic content and antioxidant activities of Azadirachta indica roots
Objective: The present study was an attempt to study total phenolic content and antioxidant property of the crude ethanolic extract of the roots of Azadirachta indica (A. indica). Materials and Methods: To evaluate the antioxidant properties of the crude extract, some complementary test systems, namely DPPH free radical scavenging assay, reducing power assay, and ferrous ion chelating ability and determination of total phenolic content were conducted. Results: In DPPH free radical scavenging test, IC50 value of the crude extract was found to be fairly significant (13.81±0.06 μg/ml) while compared with that of the reference standards, ascorbic acid and BHA (2.12±0.02 and 4.87±0.05 μg/ml, respectively). In reducing power assay, the maximum absorbance for the extract was found to be 1.523 ±0.026 at 100 μg/ml compared with standard ascorbic acid and BHA (2.811±0.013 μg/ml and 2.031±0.019 μg/ml, respectively). The IC50 value of the extract as percentage of Fe++ ion chelating ability was determined as 19.01±0.024 μg/ml where EDTA showed 8.87±0.035 μg/ml. The total phenolic amount was also calculated quite high in the extract (238.81±0.98 mg/g of gallic acid equivalent). Conclusion: The assays showed the presence of significant antioxidant properties of the crude sample, which would justify its traditional use. However, it would be very interesting to investigate the possible causes and their mechanisms responsible for the antioxidant property of the plant A. indica.
https://ajp.mums.ac.ir/article_749_a16696866fbe577364506cdd0d558127.pdf
2014-03-01
97
102
10.22038/ajp.2014.749
Azadirachta indica
Antioxidant
Activities DPPH Free Radical Scavenging
Total Penolic Content
Md. Delowar
Hossain
1
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh
AUTHOR
Md. Shahid
Sarwar
2
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh
AUTHOR
Syed Masudur
Rahman Dewan
3
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh
AUTHOR
Md. Shohel
Hossain
4
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh
AUTHOR
A. F. M.
Shahid-Ud-Daula
5
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh
AUTHOR
Mohammad
Safiqul Islam
research_safiq@yahoo.com
6
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh
LEAD_AUTHOR
Amin MN, Dewan SMR, Noor W, Shahid-Ud-Daula AFM. 2013. Characterization of chemical groups and determination of total phenolic content and in-vitro antioxidant activities of ethanolic extract of Ocimum sanctum leaves growing in Bangladesh. Euro J Exp Bio, 3: 449-454.
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12
ORIGINAL_ARTICLE
Effects of saffron (Crocus sativus) petal ethanolic extract on hematology, antibody response, and spleen histology in rats
Objective: Saffron petal is a by-product that contains flavonoids and anthocyanins. In order to study the effects of saffron petal extract (SPE) on blood parameters, immune system, and spleen histology, five treatments (n=6) were used in a completely randomized design. Materials and Methods: The treatments were 0, 75, 150, 225, and 450 mg/kg body weight of SPE. The SPE was injected intraperitoneally to 30 rats (10-week old, weighing 225±15 g) for 14 days. Immunization was performed using 1×108 sheep red blood cells (SRBC) on days 0 and 7 subcutaneously in all treatment groups. On day 15, blood was collected from the heart of rats after anesthesia. One part of samples were poured in heparinized tubes for counting whole blood cells (CBC) and different white blood cells (WBC) and the other part was used to measure IgG using ELISA technique. The spleen was stained by hematoxylin- eosin for histological study. The data were statistically analyzed using ANOVA program and the means evaluation was done using Tukey’s test. Results are presented as mean±SD. Results: Results showed no significant difference between treatments and control group regarding the amount of RBC, HGB, HCT, and PLT. The level of IgG at 75 mg/kg was significantly increased in comparison with other groups. No changes were observed in spleen histology. Conclusion: The results indicate that use of SPE at dose of 75 mg/kg causes an increase in antibody response without any change in hematological parameters and spleen histology.
https://ajp.mums.ac.ir/article_1912_0b165242fd9399503faec3513799b63e.pdf
2014-03-01
103
109
10.22038/ajp.2014.1912
Hematology
Immune system
Rat
Saffron petal extract
Atefeh
Babaei
atefeh.babaie@gmail.com
1
Department of Animal Sciences, Agriculture Faculty, Ferdowsi University of Mashhad, I. R. Iran
LEAD_AUTHOR
Javd
Arshami
arshami007@gmail.com
2
Department of Animal Sciences, Agriculture Faculty, Ferdowsi University of Mashhad, I. R. Iran
AUTHOR
Alireza
Haghparast
alireza.haghparast@gmail.com
3
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, I. R. Iran
AUTHOR
Mohsen
Daneshmesgharan
danesh@um.ac.ir
4
Department of Animal Sciences, Agriculture Faculty, Ferdowsi University of Mashhad, I. R. Iran
AUTHOR
Abdullaev FI, Espinosa Aguirre JJ. 2004. Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer detect prev, 28: 426- 432.
1
Abdullaev FI. 2001. Cancer Chemopreventive and Tumoricidal Properties of Saffron (Crocus sativus L.). Exp Biol Med, 227: 20-25.
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Abolhasani A, Bathaie SZ, Yavari I, Moosavi-Movahedi AA. 2005. Separation and purification of some components of Iraninan saffron. Asian J Chem, 2: 727- 729.
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Agrawal SS, Khadase SC, Talele GS. 2010. Studies on immunomodulatory activity of capparis Zeylanica Leaf Extracts. Int J Pharm Sci Nonotechnol, 3: 887-892.
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Akhondzadeh Basti A, Moshiri E, Noorbala AA, Jamshidi AH, Abbasi SH, Akhondzadeh S. 2007. Comparison of petal of Crocus sativus L. and fluoxetine in the treatment of depressed outpatients: A pilot double-blind randomized trial. Prog Neuropsychopharmacol Biol Psychiatry, 31: 439-442.
5
Assimopoulou AN, Sinakos Z, Papageorgiou VP. 2005. Radical Scavenging Activity of Crocus sativus L. Extract and its Bioactive Constituents. Phytother Res, 19: 996-1000.
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Das I, Chakrabarty RN, Das S. 2004. Saffron can prevent chemically induced skin carcionogenesis in swiss albino mice. Asian Pac J cancer Prev, 5: 70-76.
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Goli SAH, Mokhtari F, Rahimmalek M. 2012. Phenolic compounds and antioxidant activity from saffron (crocus sativus) petal. J Agri sci, 4: 10.
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Goupy P, Abert Vian M, Chema F, Caris-Veyrat C. 2013. Identification and quantification of flavonols, anthocyanins and lutein diesters in tepals of Crocus sativus by ultra performance liquid chromatography coupled to diode array and ion trap mass spectrometry detections. Ind Crop Prod, 44: 496-510.
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Hosseinzadeh H, Motamedshariaty V, Hadizadeh F. 2007. Antidepressant effect of Kempferol, a constituent of saffran( Crocus sativus) petal, in mice and rats. Pharmacologyonline, 2: 367-370.
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33
ORIGINAL_ARTICLE
Effect of the aqueous extract of Foeniculum vulgare (fennel) on the kidney in experimental PCOS female rats
Objective: Foeniculum vulgare seed (F. vulgare) is a herbal plant which is used with phytoestrogene compounds for polycystic ovary syndrome (PCOS) treatment. In this research, renoprotective effect of the aqueous extract of Foeniculum vulgare (AEF) in experimental PCOS female rats is studied. Materials and Methods: Forty female rats were randomly divided into five groups. The first group served as control,was injected with an equivalent volume (0.2 ml) of normal saline, and received normal diet. Animals in the second group were non poly cystic ovary syndrome (PCOS) rats which were treated with intragastric administration of aqueous extract of F. vulgare (150 mg/kg b.w.). In the third group, the rats were treated with intraperitoneal injection of estradiolvalerate (EV) (4 mg in 0.2 ml of sesame oil). The fourth groups were treated with EV and AEF (150mg/kg bw) with the same route. The fifth groups were treated with EV and AEF (100mg/kg bw). After 4 weeks of study, all of the rats were sacrificed, their kidneys tissues were processed for light microscopy, and some biochemical parameters of serum were measured. Results: The mean values of blood urea nitrogen in PCOS rats treated with low dose of AEF and EV and non-treated, was significantly (p<0.05) increased compared with non-PCOS and PCOS rats treated with high dose of AEF. Moreover, histopathological changes of kidney samples were comparable in PCOS rats with respect to treated groups with AEF. Conclusion: Aqueous extract of fennel seed showed the beneficial effect (especially at dose of 150 mg/kg b.w.) on renal function in PCOS rats.
https://ajp.mums.ac.ir/article_1824_6ed88551a54a829cd0568120d8015606.pdf
2014-03-01
110
117
10.22038/ajp.2014.1824
Estradiol-Valerate
Foeniculum vulgare
Kidney
Rat
Somayyeh
Sadrefozalayi
sfozala@gmail.com
1
Department of Biology, Faculty of science, Urmia University, Urmia, I. R. Iran
LEAD_AUTHOR
Farah
Farokhi
f.farokhi@urmia.ac.ir
2
Department of Biology, Faculty of science, Urmia University, Urmia, I. R. Iran
AUTHOR
Abdelaaty A, Shahat AA, Ibrahim AY, Hendawy SF, Omer EA, Hammouda FM, Abdel-Rahman FH and Mahmoud A. Saleh MA.2011. Chemical Composition, Antimicrobial and Antioxidant Activities of Essential Oils from Organically Cultivated Fennel Cultivars,Molecules, 16, 1366-1377.
1
Albert-Puelo M, 1980.Fennel and anise as estrogenic agents. J Ethnopharmacology, 12: 337-344. Alvarez A, Hermenegildo C, Issekutz AC, Esplugues JV, Sanz MJ, 2002. Estrogens inhibit angiotensin II-induced leukocyte-endothelial cell interactions in vivo via rapid endothelial nitric oxide synthase and cyclooxygenase activation. Circ Res, 91: 1142-1150.
2
Antus B,Hamar B,Kokeny G,Mucsi L,Nemes Z,Rosival L, 2003 .Estradiol is nephroprotective in the rat remnant kidney.Nephrol Dial Transplant ,18: 54-61.
3
Bhathena SJ and Velasquez MT, 2002. Beneficial role of dietary phytoestrogens in obesity and diabetes. Am J Clin Nutr, 76: 1191-201.
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Lau DC, Yan H, Dhillon B, 2006. Metabolic syndrome: a marker of patients at high cardiovascular risk. Can J Cardiol, 22: 85B-90B. Mirzayi F, Kazemi N. 2008. Prevalence of polycystic ovary syndrome in women with type 2 diabetes in Kerman, Iran. Metab Syndr Relat Disord, 6:215-217
19
Negulescu O, Bognar I, Lei J, Devarajan P, Silbiger S, Neugarten J. 2002. Estradiol reverses TGF-beta1-induced mesangial cell apoptosis by a casein kinase 2-dependent mechanism. Kidney Int, 62: 1989-1998.
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Ozbek H, Ugras S, Dulger H, Bayram I, Tuncer I, Ozturk G. 2003. Hepatoprotective effect of Foeniculum vulgare essential oil. Fitoterapia, 74: 317-319.
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Quan A, hakravarty S, Chen JK, Chen JC, Loleh S, Saini N, Harris RC,Capdevila J, Quigley R. 2004. Androgens augment proximal tubule transport. Am J Physiol Renal hysiol, 287: F452–F459.
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25
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26
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28
Rosenblum ER, Saubern RE. 1993. Assesmant of the estrogenic activity of phytoestrogenes isolated from bourbon and beer. Alcholelin Exp Res, 17: 1207-1209.
29
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Silbiger SR and Neugarten J. 1995. The impact of gender on the progression of chronic renal disease. Am J Kidney Dis, 25: 515-533
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32
ORIGINAL_ARTICLE
Effect of Bunium persicum aqueous extract plus endurance exercise on cardiorespiratory capacity and serum lipid profile
Objective(s): We examined the effects of endurance exercise in the presence of Bunium persicum extract administration on lipid profile and cardiorespiratory capacity in hypercholesterolemic male mice. Materials and Methods: Forty male hypercholesterolemic mice were divided into four groups: Vehicle, Endurance exercise (EE), Bunium persicum extract (BPE), and EE + BPE. The exercise protocol was performed at a speed of 18 m/min, 40 min/day, and 5 days/week for 6 weeks. The BPE was administered orally by a dose of 20 mg/Kg/day. Results: The results indicated that the 6-week endurance training accompanied by Bunium Persicum extract administration increased cardiorespiratory capacity significantly (601±39 vs. 293±20 meters, p<0.001). Total cholesterol level was significantly reduced in EE + BPE compared with Vehicle and EE groups (p<0.05). LDL-c was lower in EE + BPE compared with the Vehicle (p<0.01). HDL-c in BPE and EE + BPE groups was significantly higher than Vehicle (pConclusion: The results suggested that Bunium persicum extract is very useful in improvement of lipid profile in hypercholesterolemic animals. Supplementation of the extractto exercise significantly increased the cardiorespiratory capacity.
https://ajp.mums.ac.ir/article_2074_3a963936a180a49fd40f30f7dfcf6a2e.pdf
2014-03-01
118
126
10.22038/ajp.2014.2074
Bunium persicum extract
Cardiorespiratory capacity
Endurance Exercise
Hypercholesterolemia
lipid profile
Mohammad
Khaksari
1
Physiology Research Center and Department of Physiology, Kerman University of Medical Sciences, Kerman, I. R. Iran
AUTHOR
Mohsen
Ahmadi
mkhaksari@kmu.ac.ir
2
Department of Physical Education, Shahid Bahonar University, Kerman, I. R. Iran
AUTHOR
Hamid
Najafipour
najafipourh@yahoo.co.uk
3
Physiology Research Center and Department of Physiology, Kerman University of Medical Sciences, Kerman, I. R. Iran
LEAD_AUTHOR
Nader
Shahrokhi
shahrokhisa@yahoo.com
4
Physiology Research Center and Department of Physiology, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Al-Jarrah M, Pothakos K, Novikova L, Smirnova IV, Kurz MJ, Stehno-Bittel L, Lau YS. 2007. Endurance exercise promotes cardiorespiratory rehabilitation without neurorestoration in the chronic mouse model of parkinsonism with severe neurodegeneration. Neuroscience, 149: 28-37.
1
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2
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3
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4
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5
Asha Devi S, Prathima S, Subramanyam MV 2003. Dietary vitamin E and physical exercise: In: A. Lemhadri, L. Hajji, J.-B. Michel,, M. Eddouks. 2006. Cholesterol and triglycerides lowering activities of caraway fruits in normal and streptozotocin diabetic rats. J Ethnopharmacol, 106: 321-326.
6
Chandra M, Rajkumar M, Debidas G. 2006. Comparative Study on Antihyperglycemic and Antihyperlipidemic Effects of Separate and Composite Extract of Seed of Eugenia jambolana and Root of Musa paradisiaca in Streptozotocin-Induced Diabetic Male Albino Rat. Ir J Pharmacol Therapeut, 5, 1:27-33
7
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8
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9
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Luo QF, Sun L Si JY, Chen DH 2008. Hypocholesterolemic effect of stilbenes containing extract-fraction from Cajanus cajan L. on diet-induced hypercholesterolemia in mice. Phytomedicine. 11: 932-939
22
Moathar F, Shams-Ardakani MR. 2000. Guide in plant therapy. Academic Publication of Medical Sciences, p.39, Tehran, Iran (Book in Persian).
23
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24
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25
Roberts S, Robergs R. 2000. Fundamental principles of exercise physiology for fitness, Performance and Health:
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Essentials of Strength Training and Conditioning - 3rd Edition, McGraw-Hill.
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29
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30
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35
ORIGINAL_ARTICLE
Anti-inflammatory effect of Moringa oleifera Lam. seeds on acetic acid-induced acute colitis in rats
Objective: Anti-inflammatory, immuno-modulatory, and antioxidant properties of Moringa oleifera Lam. suggest that it might have beneficial effects on colitis. The present study was performed to investigate the anticolitis effect of Moringa oleifera seeds hydro-alcoholic extract (MSHE) and its chloroform fraction (MCF) on acetic acid-induced colitis in rats. Materials and Methods: Both MSHE and MCF with three increasing doses (50, 100, and 200 mg/kg) were administered orally to separate groups of male Wistar rats, 2 h before ulcer induction (using acetic acid 4%) and continued for 5 days. Prednisolone (4 mg/kg) and normal saline (1 ml/kg) were used in reference and control groups, respectively. All rats were sacrificed 24 h after the last dose (at day 6) and tissue injuries were assessed macroscopically and pathologically. Results: Extracts with three doses mentioned before were effective to reduce weight of distal colon (8 cm) as a marker for inflammation and tissue edema. Three doses of MSHE and two greater doses of MCF (100 and 200 mg/kg) were effective to reduce ulcer severity, area, and index as well as mucosal inflammation severity and extent, crypt damage, invasion involvement, total colitis index, and MPO activity compared with controls. MCF (50 mg/kg) was not significantly effective in reducing evaluated parameters of colitis compared with controls. Conclusion: It is concluded that MSHE and MCF were both effective to treat experimental colitis and this might be attributed to their similar major components, biophenols and flavonoids. Since the efficacy was evident even in low doses of MSHE, presence of active constituents with high potency in seeds is persuasive.
https://ajp.mums.ac.ir/article_1072_60363089750b9c601a40e25a11db8c6c.pdf
2014-03-01
127
136
10.22038/ajp.2014.1072
Acetic acid
Colitis
Inflammation
Moringa oleifera Lam
Rats
Seeds Extract
Mohsen
Minaiyan
minaiyan@pharm.mui.ac.ir
1
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R.Iran.
LEAD_AUTHOR
Gholamreza
Asghari
asghari@pharm.mui.ac.ir
2
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I. R. Iran
AUTHOR
Diana
Taheri
3
Department of Clinical Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I. R. Iran
AUTHOR
Mozhgan
Saeidi
4
Isfahan Pharmaceutical Sciences Research Center, Isfahan, I. R. Iran
AUTHOR
Salar
Nasr-Esfahani
5
School of Medicine, Isfahan University of Medical Sciences, Isfahan, I. R. Iran
AUTHOR
Anwar F, Latif S, Ashraf M, Gilani AH. 2007. Moringa oleifera Lam.: a food plant with multiple medicinal uses. Phytother Res, 21: 17-25.
1
Caceres A, Saravia A, Rizzo S, Zabala L, Leon ED, Nave F. 1992. Pharmacologic properties of Moringa oleifera. 2: Screening for antispasmodic, anti-inflammatory and diuretic activity. Ethnopharmacol, 36: 233-237.
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Cooper HS, Murthy SNS, Shah RS, Sedergran DJ. 1993. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest, 69: 238-249.
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ORIGINAL_ARTICLE
The effects of aqueous extract of Aloe vera leaves on the gastric acid secretion and brain and intestinal water content following acetic acid- induced gastric ulcer in male rats
Objective: Gut–brain axis (GBA) is very important in creation and modulation of gastrointestinal problems. Aloe vera gel has gastroprotective properties. The purpose of this study was to evaluate the effect of aqueous extract of Aloe vera leaves on the gastric acid secretion and brain and intestinal water content following acetic acid gastric ulcer induction. Materials and Methods: Gastric ulcer was induced by injection of 20% acetic acid into the subserosal layer in male rats. Rats were randomly assigned into three groups: intact group, gastric ulcer group and Aloe vera group (treatment with Aloe vera following gastric ulcer induction). The acid levels and brain and intestinal water content of each sample were measured eight days after the gastric ulcer induction. Results: Gastric acid levels were significantly decreased in Aloe vera group when compared with gastric ulcer group (p<0.05). However, there were no differences in acid output between gastric ulcer and Aloe vera groups with intact group. After Aloe vera administration, the amount of brain water content had no difference with intact and gastric ulcer groups (p<0.05). The duodenal water content in Aloe vera group was significantly reduced compared with intact group (p<0.05) but gastric ulcer group had no significant difference with intact and Aloe vera group. Conclusions: The administration of Aloe vera has an inhibitory effect on the gastric acid output.
https://ajp.mums.ac.ir/article_1909_c29256ed9e4ec6d4b06560c168c07d7b.pdf
2014-03-01
137
143
10.22038/ajp.2014.1909
Aloe vera
Brain water content
Gastric acid secretion
Peptic ulcer
Zakieh
Keshavarzi
zakieh_keshavarzi@yahoo.com
1
Department of Physiology, School of Medicine, Bojnurd University of Medical Sciences, Bojnurd, I. R. Iran
AUTHOR
Taha Mohammad
Rezapour
2
Student Research Committee, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, I. R. Iran
AUTHOR
Mehran
Vatanchian
3
Department of Physiology, School of Medicine, Bojnurd University of Medical Sciences, Bojnurd, I. R. Iran
AUTHOR
Mohammad Zare
Hesari
4
Student Research Committee, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, I. R. Iran
AUTHOR
Hadi
Nabizade Haghighi
5
Student Research Committee, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, I. R. Iran
AUTHOR
Mostafa
Izanlu
mostafaizanlu: @gmail.com
6
Student Research Committee, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, I. R. Iran
AUTHOR
Maryam
Sabaghian
maryam.sabaghian@gmail.com
7
Department of Physiology, North Khorasan University of Medical Sciences, Bojnurd, I. R. Iran
AUTHOR
Kaveh
Shahveisi
shahveisik871@mums.ac.ir
8
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
LEAD_AUTHOR
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