ORIGINAL_ARTICLE
The effects of Artemisia aucheri extract on hepatotoxicity induced by thioacetamide in male rats
Objective: Liver is an important organ that is exposed to many oxidant and carcinogenic agents, thus antioxidant compounds are beneficial for liver health. Artemisia contains flavonoid compounds and anti-diabetic, antioxidant, and anti-inflammatory properties. Due to possessing terpene and sesquiterpene compounds, this plant has antioxidant properties. This study was done to investigate the effects of Artemisia plant extract on thioacetamide-induced hepatotoxicity in Wistar rats. Materials and Methods: For induction of hepatotoxicity, 50 mg/kg thioacetamide was injected intraperitoneally (i.p).After extraction and purification, the hydroalcoholicextract was injected i.p. at 100, 200, and 300 mg/kg doses for 21 days together with thioacetamide at 50 mg/kg dose in the last 3 days. After blood sampling and separation of serum, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), albumin, and total protein concentrations were measured. Results: Significant decreases in aminotransferase and alkaline phosphatase activities and significant increases in the concentration of albumin and total protein in groups treated with the extract compared with thioacetamide-treated group were observed (p<0.05). Conclusion: The results indicate that protective effects of Artemisia extract against the thioacetamide-induced hepatotoxicity may be due to its ability to block the bioactivation of thioacetamide, primarily by inhibiting the activity of Cyp450 and free radicals. Artemisia possesses quercetin. Studies have demonstrated that quercetin inhibits lipid peroxidation and as an antioxidant can inhibit lipid peroxidation.
https://ajp.mums.ac.ir/article_766_7ab1dd93b910478ff0ee8843a7faa0a4.pdf
2013-09-01
293
301
10.22038/ajp.2013.766
Artemisia
Hepato-protective
Phenolic compounds
Rat
Thioacetamide
Azam
Rezaei
1
Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, I. R. Iran
AUTHOR
Shahnaz
ShekarForoush
shekarforoush@iaua.ac.ir
2
Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, I. R. Iran
LEAD_AUTHOR
Saeed
Changizi Ashtiyani
ashtiyani@sums.ac.ir
3
Department of Physiology, Arak University of Medical Sciences, Arak, I. R. Iran
AUTHOR
Hydar
Aqababa
4
Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, I. R. Iran
AUTHOR
Ali
Zarei
5
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, I. R. Iran
AUTHOR
Maryam
Azizi
6
Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, I. R. Iran
AUTHOR
Hasan
Yarmahmodi
7
Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, I. R. Iran
AUTHOR
Asgari S, Jaffari Dinani N, Madani H, Mahzouni P. 2008 a. Ethanolic extract of Artemisia Aucheri induces regression of aorta wall fatty streaks in hypercholesterolemic rabbits. Pharmazie, 63: 394-397.
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Zaragoza A, Ander D, Sarrion D and Cascales M. 2005. Potentiation of thioacetamide hepatotoxicity by phenobarbital pretreatment in rats. Inducibility of FAD monooxygenase system and age effect. Chem Biol Interact, 124: 87-101
41
ORIGINAL_ARTICLE
Analgesic, neuropharmacological, anti-diarrheal, and cytotoxic activities of the extract of Solanum sisymbriifolium (Lam.) leaves
Objective: The present study was undertaken to evaluate the possible analgesic, neuropharmacological, anti-diarrheal, and cytotoxic activities of the ethanol extract of leaves of Solanum sisymbriifolium Lam. (Family: Solanaceae). Materials and Methods: The analgesic activity was measured by acetic acid-induced writhing inhibition test. The neuropharmacological activities were evaluated using hole cross, hole board, and elevated plus-maze test and the anti-diarrheal activity was assessed using castor oil-induced diarrhea inhibition method. Brine shrimp lethality bioassay was carried out for assessing the cytotoxicity of the ethanol extract of the leaves. Except cytotoxic activity, all the tests were conducted on mice. Results: The extract at oral doses of 200 and 400 mg/kg body weight showed highly significant (p<0.001) decrease in number of writhing, 52.1±0.66 and 4.4±0.64 compared with the control (78.6±0.29) with the percentage of inhibitions of writhing response were found to be 33.72% and 94.40%, respectively. Compare with the control, the extract at both doses showed significant sedative effect in hole cross test. In hole board test, the extract exhibited highly significant (p<0.001) anxiolytic activity at dose of (200 mg/kg), while the same activity was observed at dose of 400 mg/kg in elevated plus-maze test. The extract showed highly significant (p<0.001) anti-diarrheal activity in a dose-dependent manner. With the extract, significant lethality to brine shrimp was found with LC50 value of 61.66±0.9 μg/ml, which was comparable with the positive control(LC50: 11.89±0.8 µg/ml). Conclusion: The results from the present studies support the traditional uses of this plant part and could form the basis of further investigation including compound isolation.
https://ajp.mums.ac.ir/article_384_2052194a2c648aeedb519fbacb21f2f6.pdf
2013-09-01
302
312
10.22038/ajp.2013.384
Analgesic
Anti-diarrheal
Anxiolytic
Cytotoxic
Sedative Activity. Solanum sisymbriifolium
Apurba Sarker
Apu
apurba2sarker@yahoo.com
1
Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh
LEAD_AUTHOR
Shakhawat Hossan
Bhuyan
2
Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh
AUTHOR
Maima
Matin
3
Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh
AUTHOR
Md. Faruq
Hossain
4
Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh
AUTHOR
Farjana
Khatun
5
Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh
AUTHOR
Abu
Taiab Md. Jamaluddin
6
Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh
AUTHOR
Adamec R, Shallow T. 2000. Effects of baseline anxiety on response to kindling of the right medial amygdale. Physiol Behav, 70: 67-80.
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25
ORIGINAL_ARTICLE
Characterization of phytoconstituents and evaluation of total phenolic content, anthelmintic, and antimicrobial activities of Solanum violaceum Ortega. (Family:Solanaceae)
Objective: The present study was conducted to detect possible chemicals (phytoconstituents), prove ethno-medicinal value of the plant, and investigate antimicrobial, anthelmintic, and total phenolic content of crude methanolic extract of the Solanum violaceum plant. Materials and Methods: Phytochemical screening was carried out using different chemical group test methods. In anthelmintic activity test (using Pheretima posthuma model), five concentrations (10, 20, 30, 40, 50, and 80 mg/ml in distilled water) of extracts and albendazole as standard were used which involved the vermifuge and vermicidal activity on the worms. For the evaluation of in vitro antimicrobial activity, disc diffusion method, and to determine the total phenolic content, Folin-Ceocalteu method (gallic acid as standard) were used. Results: The phytoconstituent analysis revealed presence of alkaloids, carbohydrate, glycoside, flavonoid, saponin, gum, diterpenes, phenol, protein, and tannin. The crude extract exhibited significant anthelmintic property comparing with the standard. The methanolic extract revealed broad-spectrum antimicrobial activity at the concentration of 400 µg/disc. The results were compared with that of the standard ciprofloxacin. The extract exhibited moderate amount of total phenolic compound (54.67±1.18 mg/gm of gallic acid equivalent). Conclusion: Since S. violaceum have shown antimicrobial, antioxidant, and anthelmintic activities, more studies such as anti-inflammatory, analgesic, antipyretic, and other pharmacological activities should be carried out to justify its traditional use, as the plant is available and used broadly in the rural areas for folkloric remedies.
https://ajp.mums.ac.ir/article_1056_5caefa7fdc03df611d15b2bfb755280e.pdf
2013-09-01
313
320
10.22038/ajp.2013.1056
Broad-spectrum
Phytoconstituents
Solanum violaceum
Total phenolic content
Vermicidal activity
Golam Sarwar
Raju
gsraju.nstu08@yahoo.com
1
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali- 3814 Bangladesh
AUTHOR
Md. Mizanur Rahman
Moghal
pharmamizan@ymail.com
2
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali- 3814, Bangladesh
LEAD_AUTHOR
Syed Masudur Rahman
Dewan
pharma.masud@hotmail.com
3
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali- 3814 Bangladesh
AUTHOR
Mohammad Nurul
Amin
amin.pharma07@gmail.com
4
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali- 3814 Bangladesh
AUTHOR
Md. Mustahsan
Billah
mustahsan04pharmacy@yahoo.com
5
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali- 3814 Bangladesh
AUTHOR
Ajaiyeoba EO, Onocha PA, Larenwaju OTO. 2001. In vitro anthelmintic properties of Buchholzia coriaceae and Gynandropsis gynandra extract. Pharm Biol, 39: 217-220.
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23
ORIGINAL_ARTICLE
Effects of combinations of curcumin, linalool, rutin, safranal, and thymoquinone on glucose/serum deprivation-induced cell death
Objective: Several phytochemical agents have been known to exhibit a neuroprotective effect. Among them, curcumin, linalool, rutin, safranal, and thymoquinonewere widely investigated and neuroprotective activity of each of them was shown by several studies. This work was planned to investigate whether different combinations of them could induce better neuroprotective effect against glucose/serum deprivation (GSD)-induced cytotoxicity. Materials and Methods: PC12 cells were cultivated for 8 h in GSD condition in both the absence and presence of curcumin, linalool, rutin, safranal, thymoquinone, or combinations of them. At the end of the experiment, the cell viability was determined using MTT assay. Results: The cells cultured in GSD condition showed a significant decrease in viability (28±1%) as compared with those cultured in standard condition (100±2%). In the presence of curcumin (10 µg/ml), linalool (16 µg/ml), rutin (200 µg/ml), safranal (50 µg/ml), and thymoquinone (1 µg/ml), the cell viability increased to 69±3.4% (p<0.001), 44±1.4% (p<0.01), 64±0.5% (p<0.001), 49±2% (p<0.001), and 70±3.2% (p<0.001), respectively. When different combinations of the agents were tested, the best cytoprotective activity was obtained from safranal + curcumin + thymoquinone (97±5%, pvs. untreated cells). Conclusions: The present study demonstrated that a combination of safranal + curcumin + thymoquinone can block GSD-induced cell death and has the potential to be considered for management of cerebral ischemia and neurodegenerative diseases.
https://ajp.mums.ac.ir/article_418_38451fc8b25111595fefc97bc54563fe.pdf
2013-09-01
321
328
10.22038/ajp.2013.418
Curcumin
Linalool
PC12
Rutin
Safranal
Thymoquinone
Bagher
Alinejad
1
1- Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
AUTHOR
Ahmad
Ghorbani
ghorbania@mums.ac.ir
2
3- Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
LEAD_AUTHOR
Hamid Reza
Sadeghnia
3
4- Neuroscience Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
AUTHOR
Abdullaev FI, Riveron-Negrete L, Caballero-Ortegaa H, Manuel Hernandez J, Perez-Lopez I, Pereda-Miranda R, Espinosa-Aquirre JJ. 2003. Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.). Toxicol in Vitro, 17: 731-736.
1
Behravan J, Hosseinzadeh H, Rastgoo A, Malekshah OM, Hessani M. 2010. Evaluation of the cytotoxic activity of crocin and safranal using potato disc and brine shrimp assays. Physiol Pharmacol, 13: 397-403.
2
Batista PA, Werner MF, Oliveira EC, Burgos L, Pereira P, Brum LF, Story GM, Santos AR. 2010. The antinociceptive effect of (-)-linalool in models of chronic inflammatory and neuropathic hypersensitivity in mice. J Pain, 11: 1222-1229.
3
Broughton BRS, Reutens DC, Sobey CG. 2009. Apoptotic mechanisms after cerebral ischemia. Stroke, 40: e331-e339.
4
Devi SL, Kannappan S, Anuradha CV. 2007. Evaluation of in vitro antioxidant activity of Indian bay leaf, Cinnamomum tamala (Buch. -Ham.) T. Nees & Eberm using rat brain synaptosomes as model system. Indian J Exp Biol, 45: 778-784.
5
Elisabetsky E, Marschner J, Souza DO. 1995. Effects of Linalool on glutamatergic system in the rat cerebral cortex. Neurochem Res, 20: 461-465.
6
Fukui H, Toyoshima K, Komaki R. 2011. Psychological and neuroendocrinological effects of odor of saffron (Crocus sativus). Phytomedicine, 18: 726-730.
7
Ghorbani A, Rakhshandeh H, Asadpour E, Sadeghnia HR. 2011. Effects of Coriandrum sativum extracts on glucose/serum deprivation-induced neuronal cell death. Avicenna J Phytomed, 2: 4-9.
8
Hadjzadeh MR, Tavakol Afshari J, Ghorbani A, Shakeri MT. 2006. The effects of aqueous extract of garlic (Allium sativum L.) on laryngeal cancer cells (Hep-2) and L929 cells in vitro. J Med Plants, 5: 41-48.
9
Hosseinzadeh H, Abootorabi A, Sadeghnia HR. 2008. Protective effect of Crocus sativus stigma extract and crocin (trans-crocin 4) on methyl methanesulfonate-induced DNA damage in mice organs. DNA Cell Biol, 27: 657-664.
10
Hosseinzadeh H, Sadeghnia HR. 2005. Safranal, a constituent of Crocus sativus (saffron), attenuated cerebral ischemia induced oxidative damage in rat hippocampus. J Pharm Pharmaceut Sci, 8: 394-399.
11
Hosseinzadeh H, Sadeghnia HR. 2007. Protective effect of safranal on pentylenetetrazol-induced seizures in the rat: Involvement of GABAergic and opioids systems. Phytomed, 14: 256-262.
12
Janbaz KH, Saeed SA, Gilani AH. 2002. Protective effect of rutin on paracetamol- and CCl4-induced hepatotoxicity in rodents. Fitoterapia, 73: 557-563.
13
Khader M, Bresgn N, Eckl PM. 2009. In vitro toxicological properties of thymoquinone. Food Chem Toxicol, 47: 129-133.
14
Khan MM, Ahmad A, Ishrat T, Khuwaja G, Srivastawa P, Khan MB, Raza SS, Javed H, Vaibhav K, Khan A, Islam F. 2009. Rutin protects the neural damage induced by transient focal ischemia in rats. Brain Res, 1292: 123-135.
15
La Casa C, Villegas I, Alarcon de la Lastra C, Motilva V, Martin Calero MJ. 2000. Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions. J Ethnopharmacol, 71: 45-53.
16
Mendonca LM¸ da Silva Machado C, Correia Teixeira CC, Pedro do Freitas LA, Pires Bianchi MD, Greggi Antunes LM. 2012. Curcumin reduces cisplatin-induced neurotoxicity in NGF-differentiated PC12 cells. NeuroToxicol, In press.
17
Mortazavian SM, Ghorbani A. 2012. Antiproliferative effect of viola tricolor on neuroblastoma cells in vitro. Aust J Med Herbalism, 24: 93-96.
18
Mortazavian SM, Ghorbani A, Hesari TG. 2012. Effect of hydro-alcoholic extract of Viola tricolor and its fractions on proliferation of uterine cervix carcinoma cells. Iran J Obst Gynecol Infertil, 15: 9-16.
19
Mousavi SZ, Bathaie SZ. 2011. Historical uses of saffron: Identifying potential new avenues for modern. Avicenna J Phytomed, 1: 57-66.
20
Mousavi SH, Tayarani-Najaran Z, Asghari M, Sadeghnia HR. 2010a. Protective effect of Nigella sativa extract and thymoquinone on serum/glucose deprivation-induced PC12 cells death. Cell Mol Neurobiol, 30: 591-598.
21
Mousavi SH, Tayarani NZ, Parsaee H. 2010b. Protective effect of saffron extract and crocin on reactive oxygen species-mediated high glucose-induced toxicology in PC12 cells. Cell Mol Neurobiol, 30: 185-191.
22
Nassiri-Asl M, Shariati-Rad S, Zamansoltani F. 2008. Anticonvulsive effects of intracerebroventricular administration of rutin in rats. Prog Neuro-Psychopharmacol Biol Psychiatry, 32: 989-993.
23
Park SY, Kim HS, Cho EK, Kwon BY, Phark S, Hwang KW, Sul D. 2008. Curcumin protected PC12 cells against beta-amyloid-induced toxicity through the inhibition of oxidative damage and tau hyperphosphorylation. Food Chem Toxicol, 46: 2881-2887.
24
Pu F, Mishima K, Irie K, Motohashi K, Tanaka Y, Orito K, Egawa T, Kitamura Y, Egashira N, Iwasaki K, Fujiwara M. 2007. Neuroprotective effects of quercetin and rutin on spatial memory impairment in an 8-arm radial maze task and neuronal death induced by repeated cerebral ischemia in rats. J Pharmacol Sci, 104: 329-334.
25
Rakhshandeh H, Sadeghnia HR, Ghorbani A. 2012. Sleep-prolonging effect of Coriandrum sativum hydro-alcoholic extract in mice. Nat Prod Res 26: 2095-2098.
26
Rui PAN, Sheng QIU, Da-xiang LU, Jun DONG. 2008. Curcumin improves learning and memory ability and its neuroprotective mechanism in mice. Chin Med J, 121: 832-839.
27
Sadeghnia HR, Farahmand SK, Asadpur E, Rakhshandeh H, Ghorbani A. 2012. Neuroprotective effect of Lactuca sativa on glucose/serum deprivation-induced cell death. Afr J Pharm Pharmacol, 6: 2464-2471.
28
Tamaddonfard E. 2012. Turmeric active substance, curcumin, enhanced apomorphine-induced yawning in rats. Avicenna J Phytomed, In press.
29
Tamaddonfard E, Erfanparast A, Hamzeh-Gooshchi N, Yousofizadeh S. 2012. Effect of curcumin, the active constituent of turmeric, on penicillin-induced epileptiform activity in rats. Avicenna J Phytomed, 2: 196-205.
30
Tarantilis PA, Tsoupras G, Polissiou M. 1995. Determination of saffron (Crocus sativus L.) components in crude plant extract using highperformance liquid chromatography-UV-visiblephotodiode-array detection-mass spectrometry. J Chromatogra, 699: 107-118.
31
ORIGINAL_ARTICLE
The effects of gallic acid on pain and memory following transient global ischemia/reperfusion in Wistar rats
Objective: It is generally agreed that most of the phenomena observed during brain ischemia and reperfusion can be explained by the damage to membrane structure. Oxidative stress is resulted in an imbalance between high consumption of oxygen and low levels of endogenous antioxidants. It is known that gallic acid (GA) is a strong antioxidant. The present study was carried out to evaluate the effect of GA on ischemia/reperfusion (I/R)-induced brain injury in rats. Materials and Methods: Wistar adult male rats weighing 200–250 g were divided into six groups as sham operated (Sh), ischemia/reperfusion (I/R) received normal saline (I+Veh), I/R groups treated with gallic acid (I+GA, 50, 100, or 200 mg/kg, orally, respectively), or with 100 mg /kg phenytoin (I+Phen). The global cerebral I/R injury was induced by occluding bilateral common carotid arteries (BCCA) for 20 min, followed by 5 days reperfusion in adult male rats. Results: It was found that administration of 100 mg/kg GA for 5 days before and 5 days after I/R induction reversed gait performance, sensorimotor disorders (p<0.01), and hypoalgesia (p<0.001) while dose of 50 mg/kg increased passive avoidance memory significantly (p<0.05). Conclusion: Our findings clearly demonstrate that GA has beneficial effects on behavioral impairments after brain injury induced by I/R. The results of this study show that GA pretreatment ameliorates cerebral ischemia/reperfusion injury and enhances the antioxidant defense against BCCA occlusion-induced I/R in rats, so it exhibits cerebroprotective property.
https://ajp.mums.ac.ir/article_671_9fa09bd7da70ad9e6ac97b6f76d9a515.pdf
2013-09-01
329
340
10.22038/ajp.2013.671
Cerebral ischemia/reperfusion
Gallic acid
Memory
Pain
Rats
Yaghoob
Farbood
1
Physiology Research Center and Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I. R. Iran
AUTHOR
Alireza
Sarkaki
sarkaki145@yahoo.com
2
Physiology Research Center, Medicinal Plants Research Center and Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I. R. Iran
LEAD_AUTHOR
Sheida
Hashemi
3
Physiology Research Center and Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I. R. Iran
AUTHOR
Mohammad Taghi
Mansouri
4
Physiology Research Center and Department of Pharmacology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I. R. Iran
AUTHOR
Mahin
Dianat
5
Physiology Research Center and Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I. R. Iran
AUTHOR
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68
ORIGINAL_ARTICLE
Maternal feeding with walnuts (Juglans regia) improves learning and memory in their adult pups
Objective: Walnut (Juglans Regia) is a domestic fruit of Iran. Walnut kernel (WK) has many beneficial constituents such as unsaturated fatty acids, antioxidants, and vitamin E. Scientific studies have shown that fatty acids and vitamin E can modulate learning and memory processes. The aim of the present work was to study effects of walnut consumption by mothers during pregnancy and lactation on learning and memory in adult rat offsprings. Materials and Methods: The animals were divided into three groups: control (fed with ordinary food, 20 g daily), gestation (fed with WK, 6% of food intake during pregnancy), and gestation and lactation (fed with WK, 6% of food intake during gestation and lactation). Morris water maze test was performed for their adult offsprings. Results: The results showed that there was a significant difference in learning and memory of rat offsprings between experimental and control groups. Conclusion: These data may indicate that feeding mothers with WK results in improvement in learning and memory of their offsprings.
https://ajp.mums.ac.ir/article_575_d6ee588ad37a7e3eac2c6893a876c68a.pdf
2013-09-01
341
346
10.22038/ajp.2013.575
Learning
Memory
Morris Water Maze
Rat
walnut
Majid
Asadi-Shekaari
Majidasadi@kmu.ac.ir
1
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, I. R. Iran
LEAD_AUTHOR
Abuzar
Karimi
abouzar_karimi@yahoo.com
2
Department of Physiology, Faculty of Biology, Islamic Azad University, Branch of Arsanjan, Arsanjan, I. R. Iran
AUTHOR
Mohammad
Shabani
shabanimoh@yahoo.com
3
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, I. R. Iran
AUTHOR
Vahid
Sheibani
vsheibani2@yahoo.com
4
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, I. R. Iran
AUTHOR
Khadije
Esmaeilpour
bahar_es63@yahoo.com
5
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, I. R. Iran
AUTHOR
Alessandri JM, Guesnet P, Vancassel S, Astorg P, Denis I, Langelier B, Aïd S, Poumès-Ballihaut C, Champeil-Potokar G, Lavialle M. 2004. Polyunsaturated fatty acids in the central nervous system: evolution of concepts and nutritional implications throughout life. Reprod Nutr Dev, 44: 509-538.
1
Asadi-Shekaari M, Kalantaripour TP, Arab Nejad F, Namazian E, Eslami A. 2012. The anticonvulsant and neuroprotective effects of walnuts on the neurons of rat brain cortex. Avicenna J Med Biotechnol, 4: 155-158.
2
Chen K, Zhang X, Wei XP, Qu P, Liu YX, Li TY. 2009. Antioxidant vitamin status during pregnancy in relation to cognitive development in the first two years of life. Early Hum Develop, 85: 421-427.
3
Herrera E. 2002. Implications of dietary fatty acids during pregnancy on placental, fetal and postnatal developmentda review. Placenta, 23: 9S-19.
4
Hooijmans CR, Van der Zee CE, Dederen PJ, Brouwer KM, Reijmer YD, van Groen T, Broersen LM, Lutjohann, D, Heerschap A, Kiliaan AJ. 2009. DHA and cholesterol containing diets influence Alzheimer-like pathology, cognition and cerebral vasculature in APPswe/PS1dE9 mice. Neurobiol Dis, 33: 482-498.
5
Innis SM. 2007. Fatty acids and early human development. Early Hum Dev, 83:761-766.
6
Kesse-Guyot E, Fezeu L, Jeandel C, Ferry M, Andreeva V, Amieva H, Hercberg S, Galan P. 2011. French adults’ cognitive performance after daily supplementation with antioxidant vitamins and minerals at nutritional doses: A post hoc analysis of the Supplementation inVitamins and Mineral Antioxidants (SU.VI.MAX) trial. Am J Clin Nutr, 94: 892-899.
7
Li L, Tsao R, Yang R, Kramer JKG, Hernandez M. 2007. Fatty acid profiles, tocopherol contents, and antioxidant activities of hearnut (Juglans ailanthifolia var. cordiformis) and Persian walnut (Juglans regia L.). J Agric Food Chem, 55: 1164-1169.
8
Morris RG. 1984. Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods, 11:47-60.
9
Polidori MC, Pratico D, Mangialasche F, Mariani E, Aust O, Anlasik T, Mang N, Pientka L, Stahl W, Sies H, Mococci P, Nelles G. 2009. High fruit intake is positively correlated with antioxidant status and cognitive performance in healthy subjects. J Alzheimers Dis, 17: 921-927.
10
Poulose SM, Bielinski DF, Shukitt-Hale B. 2013. Walnut diet reduces accumulation of polyubiquitinated proteins and inflammation in the brain of aged rats. J Nutr Biochem, 24: 912-919.
11
Raghavendra V, Kulkarni SK. 2001. Possible antioxidant mechanism in melatonin reversal of aging and chronic ethanol-induced amnesia in plus-maze and passive avoidance memory tasks. Free Radical Bio Med, 30: 595-602.
12
Santos de Souza A, Santos Rocha M, das Grac M, do Carmo T. 2012. Effects of a normolipidic diet containing trans fatty acids during perinatal period on the growth, hippocampus fatty acid profile, and memory of young rats according to sex. Nutrition, 28: 458-464.
13
Shabani M, Nazeri M, Parsania S, Razavinasab M, Zangiabadi N, Esmaeilpour K, Abareghi F. 2012a. Walnut consumption protects rats against cisplatin-induced neurotoxicity. Neurotoxicology, 33: 1314-1321.
14
Shabani M, Larizadeh MH, Parsania S, Asadi- Shekaari M, Shahrokhi N. 2012 b. Profound destructive effects of adolescent exposure to vincristine accompanied with some sex differences in motor and memory performance. Can J Physiol Pharmacol, 90: 379-386.
15
Thomas MA, Rice HB, Weinstock D, Corwin LC. 2002. Effects of againg on food intake and body composition in rats. Physiol Beh, 4-5: 487-500.
16
Tuzcu, M, Baydas G. 2006. Effect of melatonin and vitamin E on diabetes-induced learning and memory impairment in rats. Eur J Pharmacol, 537: 106-110.
17
Umezawa M, Ohta A, Tojo H, Yagi H, Hosokawa M, Takeda T. 1995. Dietary alpha-linolcnate/linoleate balance influence- learning and memory in the scnescence-accelerated mouse (SAM). Brain Res, 669: 225-233.
18
Wang XY, Zhang ZH, Li YQ, Zhao HR, Zhao YP1. 2004. Analysis of fatty acids composition and content in walnut varieties. Acta Nutrimenta Sinica, 26: 499-501.
19
Willis LM, Shukitt-Hale B, Cheng V, Joseph JA. 2009. Dose-dependent effects of walnuts on motor and cognitive function in aged rats. British J Nutr, 101: 1140-1144.7
20
Wu A, Yeng Z, Gomez-Pinilla F. 2004. The interplay between oxidative stress and brain-derived neurotrophic factor modulates the outcome of a saturated fat diet on synaptic plasticity and cognition. Eur J Neurosci, 19: 1699-1707.
21
Yehuda S, Carasso RL. 1993. Modulation of learning, pain thresholds, and thermoregulation in the rat by preparations of free purified r-linolenic and linoleic acids: Determination of optimal n-3 to omega–6 ratio. Proc Natl Acad Sci USA, 90: 10345-10349.
22
Zhang Z, Liao L, Moore J, Wua T, Wang Z. 2009. Antioxidant phenolic compounds from walnut kernels (Juglans regia L.). Food Chem, 113: 160-165.
23
ORIGINAL_ARTICLE
Sesame effects on testicular damage in streptozotocin-induced diabetes rats
Objective(s): Reproductive dysfunction is a consequence of diabetes. Diabetes is associated with changes in testicular tissue. Sesame oil contains large amounts of polyunsaturated fatty acids and lignin with antioxidant activity, vitamin E, and monounsaturated fatty acid (MUFA). The present study investigated the effects of sesame on testis histology and male reproductive parameters in streptozotocin-induced diabetic rats. Materials and Methods: Thirty mature male Wistar rats were randomly divided into three groups, i.e., control (C), diabetic-control (DC), and sesame-treated diabetic rats (SD). Diabetes was induced by a single dose of streptozotocin (65 mg/kg; i.p). The animals were treated by a single intraperitoneal sesame extract injection (100 mg/kg b.w.) once daily for 6 weeks. Results: The biochemical analysis revealed that the diabetes resulted in significant (p<0.05) reduction in spermiogenesis, testosterone, LH, and FSH levels. Light microscopic analysis showed remarkable (p<0.05) reduction in STD (seminiferous tubules diameter), SPI (spermatogenesis index) thickness of the epithelium, and significant increase in thickness of the interstitial tissue in the diabetic group compared with the control group. Simultaneous administration of the sesame could fairly up-regulate testosterone, LH, and FSH of the animals in this group. However, some differences were manifested with improved histological features as thickness of the epithelium, seminiferous tubules diameter, and spermatogenesis index. Conclusion: These data demonstrated that sesame significantly improved diabetes complication in rat testis. This study suggested that sesame might have a protective effect against oxidative stress-induced impaired testicular functions in diabetic rats.
https://ajp.mums.ac.ir/article_768_1d5893f1cebe77f0d548d04cf153810c.pdf
2013-09-01
347
355
10.22038/ajp.2013.768
Diabetes
Sesame
Spermatogenesis
Testis
Fereshteh
Khaneshi
1
Department of Biology, Faculty of science, Urmia University, Urmia, I. R. Iran
AUTHOR
Ozra
Nasrolahi
nasrolahio@yahoo.com
2
Department of Biology, Faculty of science, Urmia University, Urmia, I. R. Iran
LEAD_AUTHOR
Shahriar
Azizi
3
Department of Anatomy, School of Medicine, Mashhad University, Mashhad, I. R. Iran
AUTHOR
Vahid
Nejati
v_nejati@yahoo.com
4
Department of Comparative Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, I. R. Iran
AUTHOR
Abdullah Nejad A, Goals A, Dabiri SH, Javadi AB. 2009. Effects of garlic juice on diabetes-induced testicular damage in rats. Iran Endocrine J, 11: 443-453.
1
Aitkem RJ. Clarkson JS, Hargreave TB. Irvine DS. 1989. Analysis of the relationship between defective sperm function and generation of reactive oxygen species in case of oligospermia. J Andrology, 10: 214-220.
2
Bairy Kl, Ganesh K, Rao Y. 2009. effect of acyclovir on the sperm parameters of albino mice. Indian j physiol Pharmacol, 53: 327-333.
3
Baynes JW, Thorpe SR. 1999. Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. J Diabetes, 48: 1- 9.
4
Boskabady MH, Rakhshandah H, Afiat M, Aelami Z, Amiri S. 2006. Anti tussive effect of Plantago lanceolata in guinea pigs. Iran J Med Sci, 31: 143-146.
5
Cai L, Hales BF, Robaire B.1997. Induction of apoptosis in the germ cells of adult male rats after exposure to cyclophosphamide. Biol Reprod, 56: 1490-1497.
6
Cameron DF, Murray FT, Drylie DD. 1985.Interstitial compartment pathology and spermatogenic disruption in testes from impotent diabetic men. Anat Rec, 213: 53-62.
7
Elleuch M, Besbes SI, Roiseux O, Blecker C, Hamadi A. 2006. Quality characteristics of sesame seeds and by products. Food Chem, 103: 641-650.
8
Guneli E, Tugyan K, Ozturk H, Gumustekin M, Cilaker S, Uysal N. 2008. Effect of melatonin on testicular damage in streptozotocin-induced diabetes rats. Eur Surg Res. 40: 354-360.
9
Hosseinzadeh H, Ramzani M, Danaei AR. 2002. Antihyperglysemic effect and acute toxicity of Securiera securidaca L. seed Extracts in mice. Phytotherapy Research, 16: 745-747.
10
Jiang GY. Practical Diabetes.1st Edition. Beijing: People's Health Publishing House. 1996: 295.
11
Kang M-H, Naito M,Sakai K,Uchida K, Toshihiko E. 2000. Mode of action of sesame lignans in protecting low density lipoprotein against oxidative damage in vitro. Life Sci, 66: 161-171.
12
Kang M-H. Natio M, Tsujihara N. Osawa T. 1998.Sesamolin inhibits lipid peroxidation in rat liver and kidney. J Nutr, 128: 1018-1022.
13
Kiyanifard D, Hassanzadeh S H, Sadrkhanlo R A, Farshid, A. 2010.Study of changes Structure seminiferouse tubule and hormone changes gonadotropin diabetic rats. Medical J Med, 22: 239-248.
14
Okamura M, Watanabe T, Kashida Y, Machida N, Mitsumori K. 2004. Possible mechanisms underlying the testicular toxicity of oxfendazole in rats. Toxicol Pathol, 32: 1- 8.
15
Moody B, Heydari G, Mahmoud Zadeh C, Harati M. 2008. Study biochemistry and histology sodium tungstate protective effect on oxidative stress induced by streptozocinin the pancreasof diabeticrats. Anatomy, 22: 279-292.
16
Richburg JH. 2000. The relevance of spontaneous- and chemically induced alternation in testicular germ cell apoptosis to toxicology. Toxicol Lett, 112-113: 79-86
17
Palmeira C M, Santos D L, Seiça R, Moreno A J, Santos M S.2001.Enhanced mitochondrial testicular antioxidant capacity in Goto-Kakizaki diabetic rats: role of coenzyme Q. Am J Physiol Cell Physiol, 281: 1023-1028.
18
Rong C P. Liong C K. Chen Su T. Jen C C. Liu T_L. Cheng H. 2005. Dietary sesame reduces serum cholesterol and enhances antioxidant capacity in hypercholesterolemia. Nutr Res, 25: 559-567.
19
Roghani M, Baluchnejadmojarad T, Roghani Dehkordi F. 2011.The Sesame Lignan Sesamin Attenuates Vascular Permeability in Rats with Streptozotocin-Induced Diabetes: Involvement of Oxidative Stress . Int J Endocrinol Metab, 9: 248-252
20
Rohrbach DH, Martin GR. 1982.Structure of basement membrane in normal and diabetic tissue. Ann NY Acad Sci, 401: 2203-2211.
21
Shahreari Sh, Khaki A, Ahmadi-Ashtiani HR, Hajiaghaei R . Rezazadeh Sh. 2010. Effects of Danae racemosa on Testosterone Hormone in Experimental Diabetic Rats. J Med Plants, 9: 114-119.
22
Sancheti S, BafnaM ,Seo SY. 2010.Anti hyperglycemic, anti hyperlipidemic, and antioxidant effects of Chaenomelessinensisfruit extract in streptozotocin induced diabetic rats. Eur Food Res Technol, 231: 415-421.
23
Sankar D. Ramakrishna Rao M, Sambandam G, Pugalendi KV.2006. Apilot study of open lable sesame oil in hypertensive diabetics. J Med Food, 9: 417-421.
24
Sarkar R, Mohanmad KP,Chowdhury M. 2000. Effect of an organophosphate pestisde quinalphos on the hypothalamo-piturity-gonadal axia in adult male rats. J Reprod Fertil, 118: 29-38
25
Shetty G, Wilson G, Huhtaniemi I, Shuttlesworth GA, Reissmann T, Meistrich ML. 2000.Gonadotropin -releasing hormone analogs stimulate and testosterone inhibits the recovery ofspermatogenesis in irradiated rat. Endocrinol, 141: 1735-1745
26
Shi-Liang FENG, Shu-Hua LI, Yan WANG, Chang-Chun CHEN, Bin GAO.2001. Effect of ligustrum fruit extract on reproduction in experimental diabetic rats. Asian J Androl, 3: 71-73.
27
Shrilatha B, Muralidhara. 2007. Early oxidative stress in testis and epididymal sperm in streptozotocin-induced diabetic mice: its progression and genotoxic consequences. J Reprod Toxicol, 23: 578-87.
28
Slegtenhorst-Eegdeman KE, de Rooij DG, Verhoef-Post M, van de Kant HJ, Bakker CE, Oostra BA. 1998. Macroorchidism in FMR1 knockout mice is caused by increased Sertoli cell proliferation during testicular development. Endocrinology, 139: 156-162
29
Soudmany S,Yuvaraj S, Malini T and BalasubramanianK. 2005. Experimental diabetes has adverse effects on the differentiation of ventral prostate during sexual maturation of rats. Anat Rec A Discov Mol Cell Evol Biol. 287: 1281-1289.
30
ORIGINAL_ARTICLE
Hydroalcoholic extract of Crocus sativus effects on bronchial inflammatory cells in ovalbumin sensitized rats
Objective: Inflammation is one of the major components of asthma. Our aim was to evaluate the effects of C. sativus extract on total and differential white blood cells (WBC) count in lung lavage fluid (LLF) of ovalbumin-sensitized rats. Materials and Methods: Forty rats were divided into five groups (n = 8 for each group) as control (C), sensitized with injection and inhalation of ovalbumin (OA) alone (S), and three groups of sensitized and treated with different doses of C. sativus extract (S+50EX, S+100EX, and S+200EX groups). Total and differential WBC counts of LLF were evaluated in control, sensitized, and treated sensitized groups. Results: Total WBC count, neutrophil, and eosinophil percentage in LLF were increased in sensitized animals compared with the control group (p<0.001). Treatment of sensitized animals with all doses of the extract significantly reduced WBC number and the percentage of neutrophil and eosinophil compared with the sensitized animals (p<0.01–0.001). Conclusion: According to these results, the extract of C. sativus could be effective on alleviating lung inflammatory cells specially eosinophils in lung lavage of sensitized animals which may indicate a preventive effect of this plant on lung inflammation in asthma.
https://ajp.mums.ac.ir/article_767_80cf53b23268c235c18e245dc86dbe9e.pdf
2013-09-01
356
363
10.22038/ajp.2013.767
Asthma
Crocus sativus
Inflammation
Ovalbumin
Rat
Sensitization
WBC
Maryam
Mahmoudabady
mahmoudabadym@mums.ac.ir
1
Applied Physiology Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
AUTHOR
Ali
Neamati
aneamati@mshdiau.ac.ir
2
Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, I. R. Iran
LEAD_AUTHOR
Somayyeh
Vosooghi
s_vosooghi23@yahoo.com
3
Department of Biology, Payame Noor University, Boshruyeh, South Khorasan, I. R. Iran
AUTHOR
Heydar
Aghababa
heydar-aghababa@iaua.ac.ir
4
Department of Biology, Faculty of Science, Arsanjan Branch, Islamic Azad University, I. R. Iran
AUTHOR
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37
ORIGINAL_ARTICLE
The effect of natural adjuvants (G2, G2F) on lung inflammation of sensitized guinea pigs
Objective: The effects of natural adjuvants were examined on total and differential WBC counts in lung lavage of sensitized guinea pigs. Materials and Methods: In three sensitized groups of guinea pigs including: untreated sensitized animals (S), sensitized animals treated with adjuvant G2 (S+G2) and G2F (S+G2F) as well as non-sensitized group (C) (n=6 for each group), total and differential WBC counts of lung lavage were examined. Sensitization of animals was achieved by injection and inhalation of ovalbumin (OA). Results: The results showed increased total WBC, eosinophil, neutrophil, and basophil counts, and decreased lymphocytes in lung lavage of sensitized animals compared with the control group (pConclusion: These results indicate important preventive effects of two natural adjuvants, especially G2, on lung inflammation of sensitized guinea pigs.
https://ajp.mums.ac.ir/article_812_71fd7aa3c09f0aa3c5fe8af25100372b.pdf
2013-09-01
364
370
10.22038/ajp.2013.812
Asthma
Natural adjuvant
Sensitized animals
WBC
Ali
Neamati
aneamati@mshdiau.ac.ir
1
Department of Biology, Faculty of Science, Islamic Azad University, Mashhad Branch, I. R. Iran
AUTHOR
Mohammad Hossein
Boskabady
boskabadymh@mums.ac.ir
2
Applied Physiology Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
LEAD_AUTHOR
Saleh
Mohaghegh Hazrati
3
School of Public Health, Tehran University of Medical Sciences and Researches on Industrial Biotechnology, Tehran, I. R. Iran
AUTHOR
Mohammad Reza
Khakzad
4
Islamic Azad University, Mashhad Branch, and Zakaria Reserch Center, Mashhad, I. R. Iran
AUTHOR
Shakeeb Hassan
Moosavi
5
Faculty of Medicine, NHLI, Imperial College, London, UK
AUTHOR
Boskabady MH, Javan H, Sajadi M, Rakhshandah H. 2007. The possible prophylactic effect of Nigella sativa seed extract in asthmatic patients. Fundament Clin Pharmacol, 21: 559-566.
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4
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ORIGINAL_ARTICLE
Effect of Phaleria macrocarpa on sexual function of rats
Objectives: The purpose of current study was to determine the effect of Phaleria macrocarpa (P. macrocarpa)fruits aqueous extract on reproductive performance of adult male rats by assessing the serum testosterone level and evaluating their libido behavior. Methods and Materials: Eighteen male adult Spraque Dawley rats were divided into three groups and designated as treatment (240 mg/kg P. macrocarpaaqueous extract), negative control (distilled water), and positive control (4 mg/kg testosterone) which were supplemented through intragastric gavage for seven weeks. On the seventh week of supplementation, each of the male rats was introduced to five female rats at five different days to allow mating and observed the libido behavior. The mounting latency and mounting frequency were recorded for each mating. Results: P. macrocarpa aqueous extract significantly increased (p<0.05) the serum testosterone level and mounting frequency of male rats. However, there was no significant effect on mounting latency. Body weight was significantly lower in rats supplemented with P. macrocarpa aqueous extract compared with the control groups (p<0.05). Conclusion: P. macrocarpa showed potential value as an alternative for improving the sexual strength by increasing the level of testosterone and libido behavior. Thus, it is suggested that P. macrocarpa can improve the fertility in man.
https://ajp.mums.ac.ir/article_871_10883c4ab632d3b7a9a726645311095d.pdf
2013-09-01
371
377
10.22038/ajp.2013.871
Andropause
Llibido
Phaleria macrocarpa
Testosterone
Saadat
Parhizkar
parhizkarsa@gmail.com
1
Medicinal Plants Research Centre, Yasuj University of Medical Sciences (YUMS), Yasuj, Iran
AUTHOR
Che Zairieha Binti
Che Zainudin
2
Biomedical Department, Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia
AUTHOR
Mohammad
Aziz Dollah
mohdaziz@medic.edu.my
3
Biomedical Department, Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia
LEAD_AUTHOR
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