ORIGINAL_ARTICLE
Gastro-protective effect of Biebersteinia multifida root hydro-methanolic extract in rats with ethanol-induced peptic ulcer
Objective: Biebersteinia multifida is one of the native plants of Iran and its root is used in folk medicine. This study aimed to evaluate the gastro-protective effect of the hydro-methanolic extract of this plant's roots against ethanol-induced gastric ulcer in rats. Materials and Methods: The following five groups of seven rats were included in this study: control (C), gastric ulcer (GU), control omeprazole (CO) and two treatment groups (the latter 3 groups were rats with gastric ulcer that orally received omeprazole, 20 mg/kg, or the root extract at 150 and 300 mg/kg (BM 150and BM 300, respectively) 1 hour before ulcer induction). One hour after ulcer induction, blood sampling was performed and after sacrificing animals, the stomachs were immediately removed. Gastric mucosal injury was studied grossly to determine the number and area of gastric ulcers. The level of nitric oxide (NO) and total antioxidant capacity (TAC) in gastric mucosa as well as serum TNF-α were determined. Results: In GU group, severe mucosal injuries were observed (pThe lesions in CO and treatment groups were much milder than GU group by regarding ulcer area and number (p In treated (BM 150 and BM 300) groups, the gastric mucosal TAC and NO level were significantly higher than GU group (p Conclusion: B. multifida possesses gastro-protective effects against ethanol-induced ulcer model; this effect is at least partly related to plant’s antioxidant and NO production accelerating properties.
https://ajp.mums.ac.ir/article_12902_c0e05c3d79a5e9151fdc0d5333b14790.pdf
2019-09-01
410
418
10.22038/ajp.2019.12902
Biebersteinia multifida
Peptic ulcer
Rat
Antioxidant
Anti-inflammatory
Mahdi
Raeesi
mahdi0062@gmail.com
1
Division of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Narges
Eskandari-Roozbahani
neskandari32@gmail.com
2
Division of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Tahoora
Shomali
tshomali@shirazu.ac.ir
3
Division of Pharmacology and Toxicology, department of basic sciences, school of veterinary medicine, shiraz university, shiraz, iran
LEAD_AUTHOR
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43
ORIGINAL_ARTICLE
Effects of saffron (Crocus sativus) on sexual dysfunction among men and women: A systematic review and meta-analysis
Objective: This systematic review and meta-analysis study evaluated the effect of saffron (Crocus sativus) on sexual dysfunction and its subscales (dimensions) among men and women. Material and Methods: PubMed/Medline, ScienceDirect, Google Scholar, as well as Scientific Information Database (www.SID.ir) and Magiran (as Persian databases) were searched without any time and language restrictions. Statistical pooling was done using the random effects model. Results: A total of 5 studies comprising 173 participants were included in this systematic review and meta-analysis. The analysis showed a statistically significant positive effect of saffron on sexual dysfunction (Std diff in means=0.811; 95% CI, 0.356–1.265) and its subscales (Std diff in means=0.493; 95% CI, 0.261–0.724). Heterogeneity indexes such as Cochran Q index and indicated a heterogeneity among the included studies (Q=9:981, df:4, (p=0.041),I2=59.92%). There was no evidence of publication bias in these studies. Conclusion: In general, saffron was proven effective in improving sexual dysfunction and its subscales among participants; this effect was different on different dimensions of sexual dysfunction. Further studies are required to extend these initial findings.
https://ajp.mums.ac.ir/article_12686_0a5d6e4ca3060ef15f5b8fd39bc611e4.pdf
2019-09-01
419
427
10.22038/ajp.2019.12686
Saffron
Crocus sativus
Sexual
Sexual dysfunction
Hossein
Ranjbar
ranjbarh1@gmail.com
1
Department of nursing, school of nursing and midwifery,Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
LEAD_AUTHOR
Akram
Ashrafizaveh
ashrafizaveh@thums.ac.ir
2
Department of midwifery, school of nursing and midwifery, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
AUTHOR
Abedimanesh N, Ostadrahimi A, Bathaie SZ, Abedimanesh S, Motlagh B, Jafarabadi MA, Sadeghi MT. 2017. Effects of saffron aqueous extract and its main constituent, crocin, on health-related quality of life, depression, and sexual desire in coronary artery disease patients: a double-blind, placebo-controlled, randomized clinical trial. Iran Red Crescent Med J, 19: e13676
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Costantini E, Villari D, Filocamo MT. 2017. Female sexual function and dysfunction, pp.138-146, Springer.
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11
Kashani L, Raisi F, Saroukhani S, Sohrabi H, Modabbernia A, Nasehi AA, Ghaeli P. 2013. Saffron for treatment of fluoxetine‐induced sexual dysfunction in women: randomized double‐blind placebo‐controlled study. Hum Psychopharmacol, 28: 54-60.
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22
Modabbernia A, Sohrabi H, Nasehi AA, Raisi F, Saroukhani S, Jamshidi A, Akhondzadeh S. 2012. Effect of saffron on fluoxetine-induced sexual impairment in men: randomized double-blind placebo-controlled trial. Psychopharmacology (Berl), 223: 381-388.
23
Mohammadzadeh-Moghadam H, Nazari SM, Shamsa A, Kamalinejad M, Esmaeeli H, Asadpour A A, Khajavi A. 2015. Effects of a topical saffron (Crocus sativus L) gel on erectile dysfunction in diabetics: A randomized, parallel-group, double-blind, placebo-controlled trial. J Evid Based Complement Altern Med, 20: 283-286.
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Safarinejad M, Shafiei N, Safarinejad S. 2010. An open label, randomized, fixed-dose, crossover study comparing efficacy and safety of sildenafil citrate and saffron (Crocus sativus Linn.) for treating erectile dysfunction in men naïve to treatment. Int J Impot Res, 22: 240-250.
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Safarinejad MR, Shafiei N, Safarinejad S. 2011. A prospective double‐blind randomized placebo‐controlled study of the effect of saffron (Crocus sativus Linn.) on semen parameters and seminal plasma antioxidant capacity in infertile men with idiopathic oligoasthenoteratozoospermia. Phytother Res, 25: 508-516.
27
Scheepe JR, Alamyar M, Pastoor H, Hintzen RQ, Blok BF. 2017. Female sexual dysfunction in multiple sclerosis: Results of a survey among Dutch urologists and patients. Neurourol Urodyn, 36: 116-120.
28
Shamsa A, Hosseinzadeh H, Molaei M, Shakeri MT, Rajabi O. 2009. Evaluation of Crocus sativus L. (saffron) on male erectile dysfunction: a pilot study. Phytomedicine, 16: 690-693.
29
Sumalatha K, Kumar S, Lakshmi SM. 2010. Review on natural aphrodisiac potentials to treat sexual dysfunction. Int J Pharm Ther, 1: 6-14.
30
Tsai TF, Yeh CH, Hwang TI. 2011. Female sexual dysfunction: physiology, epidemiology, classification, evaluation and treatment. Urol Sci, 22: 7-13.
31
Vestergaard N, Søgaard, P, Torp-Pedersen C, Aasbjerg K. 2017. Relationship between treatment of erectile dysfunction and future risk of cardiovascular disease: A nationwide cohort study. Eur J Prev Cardiol, 24: 1498-1505.
32
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33
ORIGINAL_ARTICLE
Curcumin effects on myeloperoxidase, interleukin-18 and matrix metalloproteinase-9 inflammatory biomarkers in patients with unstable angina: A randomized clinical trial
Objective: Inflammation along with oxidative stress plays an important role in the development, progression, instability and rupture of coronary atherosclerotic plaques. Several studies introduced curcumin (diferuloylmethane) as a wonderful chemical in Curcuma longa (turmeric) with appropriate anti-inflammatory and antioxidant effects. The effect of curcumin on inflammatory biomarkers was assessed in several clinical trials. This study was designed to evaluate the effect of curcumin on three pro-inflammatory biomarkers in patients with unstable angina. Materials and Methods: Forty patients with unstable angina who met the inclusion criteria, participated in this double-blind randomized clinical trial. Patients were randomly divided into two groups. The patients in the treatment group received nanocurcumin 80 mg per day for 5 days and the control group received placebo 80 mg per day for five days. Blood samples were obtained before the administration, and also 1, 2 and 4 days after taking the treatment. Serum concentrations of Myeloperoxidase (MPO), matrix metalloproteinase-9 (MMP-9) and interleukin 18 (IL-18) biomarkers were measured by ELISA. Results: There was no significant difference in concentration of these biomarkers before the administration and 1, 2 and 4 days after the start of the trial, between the two groups; however, the concentration of IL-18 on the first day significantly varied between the groups. Conclusion: Based on the findings of this study, administration of nanocurcumin capsules at the dose of 80 mg per day for 5 days, did not significantly decrease inflammatory biomarkers in patients with unstable angina.
https://ajp.mums.ac.ir/article_12644_fee71c2082c660af6ee96286e33aac36.pdf
2019-09-01
428
435
10.22038/ajp.2019.12644
Curcumin
Interlukein-18
Myeloperoxidase
Matrix metalloproteinase-9
Unstable angina
Amir hooshang
Mohammad pour
mohammadpouras11@mums.ac.ir
1
Department of Clinical Pharmacy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
Mostafa
Dastani
dastanim11@mums.ac.ir
2
Department of Cardiology, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
Roshanak
Salari
salarir@mums.ac.ir
3
Department of Clinical Persian Pharmacy, School of Persian and Complementary Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
Sohrab
Radbin
radbins11@mums.ac.ir
4
Pharmaceutical Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
Soghra
Mehri
mehris11@mums.ac.ir
5
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Maryam
Ghorbani
ghorbania11@mums.ac.ir
6
Department of Pharmacology and Toxicology, School of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran.
AUTHOR
Asieh
Karimani
karimania921@mums.ac.ir
7
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
Masoumeh
Salari
salarim@mums.ac.ir
8
Department of Internal Medicine, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
LEAD_AUTHOR
Aggarwal BB, Harikumar KB. 2009. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol, 41: 40-59.
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Rosso R, Roth A, Herz I, Miller H, Keren G, George J. 2005. Serum levels of interleukin-18 in patients with stable and unstable angina pectoris. Int J Cardiol, 98: 45-48.
23
Saja K, Babu MS, Karunagaran D, Sudhakaran PR. 2007. Anti-inflammatory effect of curcumin involves downregulation of MMP-9 in blood mononuclear cells. Int Immunopharmacol, 7: 1659-1667.
24
Sharma RA, Euden SA, Platton SL, Cooke DN, Shafayat A, Hewitt HR, Marczylo TH, Morgan B, Hemingway D, Plummer SM , Pirmohamed M, Gescher AJ, Steward WP. 2004. Phase I clinical trial of oral curcumin: biomarkers of systemic activity and compliance. Clin Cancer Res, 10: 6847-6854.
25
Sokolove J, Lepus CM. 2013. Role of inflammation in the pathogenesis of osteoarthritis: latest findings and interpretations. Ther Adv Musculoskelet Dis, 5: 77-94.
26
Sprague AH, Khalil RA. 2009. Inflammatory cytokines in vascular dysfunction and vascular disease. Biochem Pharmacol, 78: 539-552.
27
Storka A, Vcelar B, Klickovic U, Gouya G, Weisshaar S, Aschauer S, Bolger G, Helson L, Wolzt M. 2015. Safety, tolerability and pharmacokinetics of liposomal curcumin in healthy humans. Int J Clin Pharmacol Ther, 53: 54-65.
28
Swarnakar S, Ganguly K, Kundu P, Banerjee A, Maity P, Sharma AV. 2005. Curcumin regulates expression and activity of matrix metalloproteinases 9 and 2 during prevention and healing of indomethacin-induced gastric ulcer. J Biol Chem, 280: 9409-9415.
29
ZakynthinosE, Pappa N. 2009. Inflammatory biomarkers in coronary artery disease. J Cardiol, 53: 317-333.
30
Zhou H, Beevers CS, Huang S. 2011. The targets of curcumin. Curr Drug Targets, 12: 332-347.
31
ORIGINAL_ARTICLE
Effects of saffron on homocysteine, and antioxidant and inflammatory biomarkers levels in patients with type 2 diabetes mellitus: a randomized double-blind clinical trial
Objective: Type 2 diabetes mellitus (T2DM) is one of the most common health problems worldwide. Studies have shown that saffron and its derivatives may have therapeutic potentials in T2DM through reducing plasma glucose. The present study aimed to evaluate the effects of saffron extract on serum anti-inflammatory and antioxidant variables in T2DM patients. Materials and Methods: This was a double-blind randomized clinical trial conducted on 64 T2DM patients. Participants received either 15 mg of saffron or placebo capsules (two pills per day) for 3 months Anthropometric indices, homocysteine, serum anti-inflammatory and antioxidant variables and dietary intake were assessed pre- and post-intervention. Results: After 3 months of treatment, interleukin-6 (IL-6), and tumor necrosis factor (TNF-α) increased significantly in both group (p<0.05). No significant differences were observed for total antioxidant capacity (TAC), malondialdehyde (MDA),highsensitivity C-reactive protein (hs-CRP) and interleukin 10(IL-10) after the treatment period (p>0.05). Homocysteine decteased significantly in control group (p<0.05). Conclusion: Our results showed no improvement in homocystein levels, antioxidant status and inflammatory biomarkers in T2DM patients after treatment with saffron.
https://ajp.mums.ac.ir/article_12854_a6e0f2da28ff36d3fa6d48f4f06b2873.pdf
2019-09-01
436
445
10.22038/ajp.2019.12854
Inflammatory
Antioxidant
Saffron
Type 2 diabetes mellitus
Biomarkers
Hajieh
Shahbazian
hjb.shahbazian@gmail.com
1
Diabetes Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
AUTHOR
Armaghan
Moravej Aleali
a.m.aleali69@gmail.com
2
Diabetes Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
LEAD_AUTHOR
Reza
Amani
amani-r@ajums.ac.ir
3
Department of Clinical Nutrition, School of Nutrition & Food Science, Food security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
AUTHOR
Foroogh
Namjooyan
namjoyan@yahoo.com
4
Department of Pharmacognosy, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
AUTHOR
Bahman
Cheraghian
cheraghain2000@yahoo.com
5
Department of Epidemiology & Biostatistics, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
AUTHOR
Seyed Mhmoud
Latifi
sml1381@yahoo.com
6
Diabetes Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
AUTHOR
Sara
Bahrainian
s-bahrainian@razi.tums.ac.ir
7
Aerosol Research Laboratory, Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
AUTHOR
Ataallah
Ghadiri
ata.ghadiri@hotmail.fr
8
Cellular and molecular research center, Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
AUTHOR
Abbasnezhad A, Hayatdavoudi P, Niazmand S. Mahmoudabady M. 2015. The effects of hydroalcoholic extract of Nigella sativa seed on oxidative stress in hippocampus of STZ-induced diabetic rats. Avicenna Jphytomed, 5: 333-340
1
Aghamohammadi V, Pourghassem GargarI B. Aliasgharzadeh A. 2011. Evaluation of the level of plasma homocysteine in patients with type 2 diabetes mellitus under metformin treatment and its correlation with serum total antioxidant capacity, malondialdehyde, creatinine, insulin resistance and glycemic control. ZUMS J, 19: 1-10.
2
Ahangarpour A, Heidari H, Oroojan AA, Mirzavandi F, Esfehani KN, Mohammadi ZD. 2017. Antidiabetic, hypolipidemic and hepatoprotective effects of Arctium lappa root’s hydro-alcoholic extract on nicotinamide-streptozotocin induced type 2 model of diabetes in male mice. Avicenna J Phytom, 7: 169-179.
3
Akhondzadeh S, Sabet MS, Harirchian M, Togha M, Cheraghmakani H, Razeghi S, Hejazi SS, Yousefi M, Alimardani R, Jamshidi A. 2010. Saffron in the treatment of patients with mild to moderate Alzheimer’s disease: a 16‐week, randomized and placebo‐controlled trial. J Clin Pham Ther, 35: 581-588.
4
Azimi P, Ghiasvand R, Feizi A, Hariri M. Abbasi B. 2014. Effects of cinnamon, cardamom, saffron, and ginger consumption on markers of glycemic control, lipid profile, oxidative stress, and inflammation in Type 2 diabetes patients. Rev Diabet Stud, 11: 258-266.
5
Beji RS, Khemir S, Wannes WA, Ayari K, Ksouri R. 2018. Antidiabetic, antihyperlipidemic and antioxidant influences of the spice cinnamon (Cinnamomum zeylanicumon) in experimental rats. Braz J Pharm Sci, 54:1-8.
6
Bharti S, Golechha M, Kumari S, Siddiqui, KM, Arya DS. 2012. Akt/GSK-3beta/eNOS phosphorylation arbitrates safranal-induced myocardial protection against ischemia-reperfusion injury in rats. Eur J Nutr,51:719-727.
7
Bolhassani A, Khavari A. Bathaie S. 2014. Saffron and natural carotenoids: Biochemical activities and anti-tumor effects. Biochim Biophys Acta, 1845:20-30.
8
Fadai F, Mousavi B, Ashtari Z, Farhang S, Hashempour S, Shahhamzei N, Bathaie SZ. 2014. Saffron aqueous extract prevents metabolic syndrome in patients with schizophrenia on olanzapine treatment: a randomized triple blind placebo controlled study. Pharmacopsychiatry, 47: 156-161.
9
Farkhondeh T, Samarghandian S. 2014. The effect of saffron (Crocus sativus L.) and its ingredients on the management of diabetes mellitus and dislipidemia. Afr J Pharm Pharmacol, 8:541-549.
10
Ghorbani A. 2013. Best herbs for managing diabetes: A review of clinical studies. Braz J Pharm, 49:413-422.
11
Gilbert MP, Pratley RE. 2009. Efficacy and safety of incretin-based therapies in patients with type 2 diabetes mellitus. Am J Med, 122: S11-24.
12
Hemmati M, Zohoori E, Mehrpour O, Karamian M, Asghari S, Zarban A, Nasouti R. 2015. Anti-atherogenic potential of jujube, saffron and barberry: anti-diabetic and antioxidant actions. Excli J, 14: 908-915.
13
Hosseini A, Shafiee-nick R, Ghorbani A. 2015. Pancreatic beta cell protection/regeneration with phytotherapy. Braz J Pharm, 51: 1-16.
14
Hosseinzadeh H, Modaghegh MH, Saffari Z. 2009. Crocus sativus L. (Saffron) extract and its active constituents (crocin and safranal) on ischemia-reperfusion in rat skeletal muscle. Evid Based Complement Alternat Med, 6: 343-350.
15
Hosseinzadeh H, Sadeghnia HR. 2005. Safranal, a constituent of Crocus sativus (saffron), attenuated cerebral ischemia induced oxidative damage in rat hippocampus. J Pharm Pharm Sci, 8: 394-399.
16
Hosseinzadeh H, Sadeghnia HR, Ziaee T, Danaee A. 2005. Protective effect of aqueous saffron extract (Crocus sativus L.) and crocin, its active constituent, on renal ischemia-reperfusion-induced oxidative damage in rats. J Pharm Pharm Sci, 8: 387-393.
17
Jelodar G, Javid Z, Sahraian A, Jelodar S. 2018. Saffron improved depression and reduced homocysteine level in patients with major depression: A Randomized, double-blind study. Avicenna J Phytomed, 8: 43-50.
18
Kianbakht S, Hagiaghaee R. 2011. Anti-hyperglycemic effects of saffron and its active constituents, crocin and safranal, in alloxan-induced diabetic rats. J Med Plant, 3: 82-89.
19
Makhlouf H, Saksouk M, Habib J, Chahine R. 2011. Determination of antioxidant activity of saffron taken from the flower of Crocus sativus grown in Lebanon. Afr J Biotechnol, 10: 8093-8100.
20
Maleki-saghooni N, Mirzaeii K, Hosseinzadeh H, Sadeghi R, Irani M. 2018. A systematic review and meta-analysis of clinical trials on saffron (Crocus sativus) effectiveness and safety on erectile dysfunction and semen parameters. Avicenna J Phytomed, 8: 198-209.
21
Mard SA, Nikraftar Z, Farbood Y, Mansouri E. 2015. A preliminary study of the anti-inflammatory and anti-apoptotic effects of crocin against gastric ischemia-reperfusion injury in rats. Braz J Pharm, 51: 637-642.
22
Milajerdi A, Djazayeri A, Jazayeri S, Hashemzadeh N, Shirzadi E, Derakhshan Z, Akhondzadeh S. 2016. The effect of hydro-alcoholic extract of saffron stigma (crocus sativus l.) on metabolic control parameters, liver enzymes, and renal function parameters in type 2 diabetic patients. J Med Plant, 4:142-151.
23
Milajerdi A, Jazayeri S, Hashemzadeh N, Shirzadi E, Derakhshan Z, Djazayeri A, Akhondzadeh S. 2018. The effect of saffron (Crocus sativus L.) hydroalcoholic extract on metabolic control in type 2 diabetes mellitus: A triple-blinded randomized clinical trial. J Res Med Sci, 23:16- 22.
24
Mohamadpour AH, Ayati Z, Parizadeh MR, Rajbai O, Hosseinzadeh H. 2013. Safety evaluation of crocin(a constituent of saffron) tablets in healthy volunteers. Iran J Basic Med Sci, 16: 39–46.
25
Mohammad R, Daryoush M, Ali R, Yousef D, Mehrdad N. 2011. Attenuation of oxidative stress of hepatic tissue by ethanolic extract of saffron (dried stigmas of Crocus sativus L.) in streptozotocin (STZ)-induced diabetic rats. Afr J Pharm Pharmacol, 5:2166-2173.
26
Moller DE. 2000. Potential role of TNF-α in the pathogenesis of insulin resistance and type 2 diabetes. TEM, 11: 212-217.
27
Rezaee R, Hosseinzadeh H. 2013. Safranal: from an aromatic natural product to a rewarding pharmacological agent. Iran J Basic Med Sci, 16: 12-26.
28
Riahi-zanjani B, Balali-mood M, Mohammadi E, Badie-bostan H, Memar B, Karimi G. 2015. Safranal as a safe compound to mice immune system. Avicenna J Phytomed, 5:441-449.
29
Samarghandian S, Azimi-nezhad M, Samini F. 2014. Ameliorative effect of saffron aqueous extract on hyperglycemia, hyperlipidemia, and oxidative stress on diabetic encephalopathy in streptozotocin induced experimental diabetes mellitus. Biomed Res Int, 2014:1-12.
30
Samarghandian S, Borji A, Delkhosh MB, Samini F. 2013. Safranal treatment improves hyperglycemia, hyperlipidemia and oxidative stress in streptozotocin-induced diabetic rats. J Pharm Pharm Sci, 16:352-362.
31
Saxena M, Srivastava N, Banerjee M. 2013. Association of IL-6, TNF-alpha and IL-10 gene polymorphisms with type 2 diabetes mellitus. Mol Biol Rep, 40:6271-6279.
32
Wani BA, Hamza AKR, Mohiddin F. 2011. Saffron: A repository of medicinal properties. J Med Plant Res, 5: 2131-2135.
33
Yazdanpanah L, Shahbazian H, Aleali AM, Jahanshahi A, Ghanbari S, Latifi S. 2016. Prevalence, awareness and risk factors of diabetes in Ahvaz (South West of Iran). Diabetes Metab Syndr, 10: S114-S118.
34
ORIGINAL_ARTICLE
Gummosin, a sesquiterpene coumarin from Ferula assa-foetida is preferentially cytotoxic to human breast and prostate cancer cell lines
Objective: The present study was conducted to find cytotoxic compounds from oleo-gum-resin of Ferula assa-foetida (asafoetida). Materials and Methods: A dichloromethane extract of asafoetida was subjected to different chromatography analyses (including column chromatography, preparative thin layer chromatography and high performance liquid chromatography) to isolate its bioactive sesquiterpene coumarins. The structures of isolated compounds were elucidated through 1H-NMR spectra interpretation and comparison with those reported in the literature. To measure the cytotoxic activity of pure compounds, a non-fluorescent substrate called resazurin (alamarBlue®)was used in this study. Human breast and prostate cancer cell lines (MCF-7 and PC-3, respectively) and a normal human embryonic stem cell (NIH) were treated with different concentrations (50, 25, 12.5 and 6.25 µg/mL) of pure compounds. Results: In this study, 10 sesquiterpene coumarins were isolated from oleo-gum-resin of F. assa-foetida and cytotoxic activity of 6 compounds was tested against MCF-7 and PC-3 cell lines and NIH cells. Badrakemin acetate (7), ferukrinone (8) and deacetyl kellerin (10) were found for the first time in the oleo-gum-resin of F. assa-foetida. Gummosin (4) showed moderate cytotoxic activity with IC50 values of 30 and 32.1 µg/mL against PC-3 and MCF-7 cell lines, respectively. None of the isolated compounds showed toxicity against NIH as a normal human cell line. Conclusion: The preferential cytotoxic activity of gummosin against cancer cell lines is reported for the first time in this study.
https://ajp.mums.ac.ir/article_12598_71632e9abbd2635c696c52a60bf6a6de.pdf
2019-09-01
446
453
10.22038/ajp.2019.12598
Ferula assa-foetida
Apiaceae
Gummosin
Cytotoxicity
MCF-7
PC-3
Milad
Iranshahi
iranshahiml@mums.ac.ir
1
Department of Pharmacology and Toxicology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.
AUTHOR
Faegheh
Farhadi
farhadif951@mums.ac.ir
2
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
Babak
Paknejad
drpakb@yahoo.com
3
Department of Pharmacology and Toxicology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
AUTHOR
Parvin
Zareian
zareianj2011@yahoo.com
4
Department of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran
AUTHOR
Mehrdad
Iranshahi
iranshahim@mums.ac.ir
5
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
Masoumeh
Karami
karami1999@gmail.com
6
Biochemistry Department, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.
AUTHOR
Seyed Reza
Abtahi
seyrezabt@gmail.com
7
Department of Pharmacology and Toxicology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.
LEAD_AUTHOR
Akaberi M, Iranshahy M, Iranshahi M. 2015. Review of the traditional uses, phytochemistry, pharmacology and toxicology of giant fennel (Ferula communis L. subsp. communis). Iran J Basic Med Sci, 18:1050-1062.
1
Alam M, Khan A, Wadood A, Khan A, Bashir S, Aman A, Jan AK, Rauf A, Ahmad B,Khan AR. 2016. Bioassay-guided isolation of sesquiterpene coumarins from Ferula narthex Bioss: a new anticancer agent. Front Pharmacol, 7:26-32.
2
Aldaghi L, Rad A, Arab A, Kasaian J, Iranshahi M, Sadr AS, Soltani F. 2016. In silico and in vitro evaluation of cytotoxic activities of farnesiferol c and microlobin on MCF-7, HeLa and KYSE cell lines. Drug Res (Stuttg), 66:532-538.
3
Alshammari S. 2016. Cytotoxic activity of ferula assa-foetida and Astragalus sarcocolla against cervical epithelial and colon carcinoma cell lines. ETD Collection for Tennessee State University. Paper AAI10119061.
4
Bagheri SM, Asl AA, Shams A, Mirghanizadeh-Bafghi SA, Hafizibarjin Z. 2017. Evaluation of cytotoxicity effects of Oleo-Gum-Resin and its essential oil of Ferula assa-foetida and ferulic acid on 4T1 breast cancer cells. Indian J Med Paediatr Oncol, 38:116-120.
5
Hasanzadeh D, Mahdavi M, Dehghan G, Charoudeh HN. 2017. Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line. Toxicol Rep, 4:420-426.
6
Iranshahi M, Amin G, Shafiee A. 2004. A new coumarin from Ferula persica. Pharm Biol, 42:440-442.
7
Iranshahi M, Masullo M, Asili A, Hamedzadeh A, Jahanbin B, Festa M, Capasso A,Piacente S. 2010. Sesquiterpene coumarins from Ferula gumosa. J Nat Prod, 73:1958-1962.
8
Iranshahi M, Rezaee R, Najaf Najafi M, Haghbin A,Kasaian J. 2018. Cytotoxic activity of the genus Ferula (Apiaceae) and its bioactive constituents. Avicenna J Phytomed, 8:296-312.
9
Iranshahy M, Iranshahi M. 2011. Traditional uses, phytochemistry and pharmacology of asafoetida (Ferula assa-foetida oleo-gum-resin)-a review. J Ethnopharmacol, 134:1-10.
10
Kasaian J, Iranshahy M, Iranshahi M. 2014. Synthesis, biosynthesis and biological activities of galbanic acid - A review. Pharm Biol, 52:524-531.
11
Kasaian J, Mosaffa F, Behravan J, Masullo M, Piacente S, Ghandadi M, Iranshahi M. 2015. Reversal of P-glycoprotein-mediated multidrug resistance in MCF-7/Adr cancer cells by sesquiterpene coumarins. Fitoterapia, 103:149-154.
12
Kaur M, Kohli S, Sandhu S, Bansal Y, Bansal G. 2015. Coumarin: a promising scaffold for anticancer agents. Anticancer Agents Med Chem, 15:1032-1048.
13
Kim K-H, Lee H-J, Jeong S-J, Lee H-J, Lee E-O, Kim H-S, Zhang Y, Ryu S-Y, Lee M-H, Lü J. 2011. Galbanic acid isolated from Ferula assafoetida exerts in vivo anti-tumor activity in association with anti-angiogenesis and anti-proliferation. Pharm Res, 28:597-609.
14
Lee CL, Chiang LC, Cheng LH, Liaw CC, Abd El-Razek MH, Chang FR, Wu YC. 2009. Influenza A (H(1)N(1)) antiviral and cytotoxic agents from Ferula assa-foetida. J Nat Prod, 72:1568-1572.
15
Shafri M, Affendi M, Yusof FA, Zain M, Zulkarami A. 2015. In vitro cytotoxic activity of Ferula assafoetida on osteosarcoma cell line (HOS CRL). J Teknologi, 77:7-11.
16
Shakeri A, Iranshahy M, Iranshahi M. 2014. Biological properties and molecular targets of umbelliprenin--a mini-review. J Asian Nat Prod Res, 16:884-889.
17
Valiahdi S M, Iranshahi M, Sahebkar A. 2013. Cytotoxic activities of phytochemicals from Ferula species. DARU J Pharm Sci, 21:39-46.
18
van Haaften C, Duke CC, Weerheim AM, Smit NP, van Haard PM, Darroudi F, Trimbos BJ. 2011. Potent cytotoxic effects of Calomeria amaranthoides on ovarian cancers. J Exp Clin Cancer Res, 30:29-29.
19
ORIGINAL_ARTICLE
The anti-diabetic and antioxidant effects of a combination of Commiphora mukul, Commiphora myrrha and Terminalia chebula in diabetic rats
Objective: Effects of Commiphora mukul and Commiphora myrrha ethanolic extracts and Terminalia chebula hydro-ethanolic extract combination were evaluated in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: Male Wistar rats (n=48) were randomly assigned into: control; diabetic; diabetic+metformin (300 mg/kg); diabetic+dose 1 of herbal combination (438 mg/kg of C. mukul+214 mg/kg of C. myrrha+857 mg/kg of T. chebula); diabetic+dose 2 (642 mg/kg of C. mukul+214 mg/kg of C. myrrha+642 mg/kg of T. chebula); and diabetic+dose 3 (857 mg/kg of C. mukul+438 mg/kg of C. myrrha+1714 mg/kg t of T. chebula). All treatments were given orally by gavage. Diabetes was induced by STZ (60 mg/kg, i.p.). At the end of study (day 28), blood glucose, insulin and lipid profile; as well as hepatic malondialdehyde (MDA) and thiol content, and superoxide dismutase (SOD) and catalase (CAT) activities were determined. Results: In diabetic rats, plasma glucose, triglycerides (TG), total cholesterol (TC), and LDL-C, as well as hepatic MDA levels were elevated but plasma HDL-C and insulin, and hepatic thiol content and SOD and CAT activities were reduced compared to control (p<0.01-p<0.001). In diabetic+dose 3, plasma TC, TG, and LDL-C and hepatic MDA level decreased (p<0.001), while plasma HDL-C and insulin, and hepatic thiol content, and SOD and CAT activities increased compared to diabetic (p<0.01-p<0.001). Treatment with dose 1 and 2 improved such abnormalities in diabetic rats except for insulin level (p<0.05-p<0.001). The herbal combination effects were comparable to those of metformin. Metformin did not significantly change serum insulin and HDL-C levels, and hepatic SOD activity; however, serum levels of TC, TG, and LDL-C, as well as hepatic MDA levels, thiol content and CAT activity were improved compared to diabetic (p<0.05-p<0.001). Conclusion: These results indicate that this herbal combination acts as an anti-diabetic, antioxidant and hypolipidemic agent and it may be suggested as a beneficial remedy for diabetic patients.
https://ajp.mums.ac.ir/article_12721_5106e92e21114b92248513a1b20cd5dc.pdf
2019-09-01
454
464
10.22038/ajp.2019.12721
Anti-diabetic
Hypolipidemic
Antioxidant
Commiphora mukul
Commiphora myrrha
Terminalia chebula
Reyhaneh
Sotoudeh
sotoudehr951@mums.ac.ir
1
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
Mousa-al-reza
Hajzadeh
hajzadehmr@mums.ac.ir
2
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
LEAD_AUTHOR
Zahra
Gholamnezhad
gholamnezhadz@mums.ac.ir
3
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
LEAD_AUTHOR
Azita
Aghaee
aghaeea1@mums.ac.ir
4
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
Aebi H. 1984. Catalase in vitro. Methods Enzymol, 105:121-126.
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Ahmadi-Naji R, Heidarian E, Ghatreh-Samani K. 2017. Evaluation of the effects of the hydroalcoholic extract of Terminalia chebula fruits on diazinon-induced liver toxicity and oxidative stress in rats. Avicenna J Phytomed, 7: 454-456.
2
Bellamkonda R, Rasineni K, Singareddy SR, Kasetti RB, Pasurla R, Chippada AR, Desireddy S. 2011. Antihyperglycemic and antioxidant activities of alcoholic extract of Commiphora mukul gum resin in streptozotocin induced diabetic rats. Pathophysiology, 18:255-261.
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Kannan P, Ramadevi S, Hopper W. 2009. Antibacterial activity of Terminalia chebula fruit extract. African J Microbiol Res, 3:180-184.
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Kaveh M, Eidi A, Nemati A and Boskabady MH. 2017. The extract of Portulaca oleracea and its constituent, alpha linolenic acid affects serum oxidant levels and inflammatory cells in sensitized rats. Iran J Allergy Asthma Immunol, 16:256-270.
18
Kim J-h, Hong C-O, Koo Y-c, Kim S-J, Lee K-W. 2011. Oral administration of ethyl acetate-soluble portion of Terminalia chebula conferring protection from streptozotocin-induced diabetic mellitus and its complications. Biol Pharm Bull, 34:1702-1709.
19
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50
ORIGINAL_ARTICLE
Zataria multiflora could improve hippocampal tau protein and TNFα levels and cognitive behavior defects in a rat model of Alzheimer's disease
Objective: Zataria multiflora (ZM) is a plant with ethnopharmacological value which was recently tested to reduce symptoms of Alzheimer’s disease (AD). The aim of the present study was to determine the effect of ZM essential oil on spatial cognitive and noncognitive behavior, as well as hippocampal tau protein and tumor necrosis factor alpha (TNFα) concentrations in rats with AD. Materials and Methods: Thirty-five adult male Sprague Dawley rats (300±30 g) were randomly divided into 5 groups: control (intact rats); sham (received intracerebroventricular (ICV) microinjection of normal saline); AD control (rats with AD that did not receive any treatment); vehicle control (rats with AD that orally received tween-80, 5% (ZM essential oil vehicle) for 20 days) and experimental (rats with AD that orally received ZM essential oil 100 µl/kg/day for 20 days). AD was induced by bidirectional microinjection of β amyloid 1-42 (10 µg/2µl). Tau protein and TNFα concentrations were measured by ELISA methods. Spatial cognitive and noncognitive behavior were determined by Morris water maze (MWM) test. Results: ZM essential oil significantly improved latency time, time spent in the target quarter and cognitive behavior of rats with AD compared to control and sham groups (p<0.05). Hippocampal tau protein and TNFα concentrations were significantly higher in both AD control and vehicle groups compared to control and sham groups respectively (p Conclusion: ZM essential oil improves spatial learning and memory of rats with AD as assessed by MWM test. These effects are associated with decreased concentrations of hippocampal tau protein and TNFα.
https://ajp.mums.ac.ir/article_12839_209628ad11c68f6689ec558be5ece6f9.pdf
2019-09-01
465
473
10.22038/ajp.2019.12839
Alzheimer’s disease
Zataria multiflora
Morris Water Maze
Tau protein
TNFα
Mohabbat
Ahmadi
ahmadi@gmail.com
1
Physiology division of Basic Science Department, School of Vet Med; Shiraz University, Shiraz—Iran
AUTHOR
Mahnaz
Taherianfard
taherian@shirazu.ac.ir
2
Physiology division of Basic Science Department, School of Vet Med; Shiraz University, Shiraz, Iran.
LEAD_AUTHOR
Tahora
Shomali
shomali@shirazu.ac.ir
3
Pharmacology division of Basic Science Department, School of Vet Med; Shiraz University, Shiraz—Iran
AUTHOR
Adlard PA, Tran BA, Finkelstein DI, Desmond PM, Johnston LA, Bush AI, Egan GF. 2014. A review of beta-amyloid neuroimaging in Alzheimer's disease. Front Neurosci, 8:1-23.
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3
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4
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19
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21
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22
Sajed H, Sahebkar A, Iranshahi M. 2013. Zataria multiflora Boiss. (Shirazi thyme)--an ancient condiment with modern pharmaceutical uses. J Ethnopharmacol, 145:686-698.
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26
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28
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29
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30
ORIGINAL_ARTICLE
Antimalarial activity of extract and fractions of Castanopsis costata (Blume) A.DC
Objective: One of the biggest health problems in the world, which occurs in more than 90 countries, is the spread of malaria. Cep-cepan leaves (Castanopsis costata), was empirically used as an antimalarial herb in North Sumatra. Since its use has not been scientifically studied, we investigated the antimalarial activity of extract and fractions of C. costata against Plasmodium berghei ANKA (PbA) in a mouse model. Materials and Methods: This experimental study was conducted using 32 male Balb/C mice. PbA inoculation was performed intraperitoneally with 106 parasites/mouse. Immediately after parasitemia reach >2% (day 0), the mice were treated orally with daily artesunate (36.4 mg/kg/day) (positive control), ethanolic extract (100, 200, and 400 mg/kg/day), and the fractions of water, ethyl acetate and n-hexane (108 mg/kg/day each) for 5 consecutive days (from day 0 to 4). Parasitemia inhibition was observed to determine the antimalarial activity of each type of C. costata extract and fractions. Results: The administration of C. costata leaves ethanolic extract (100, 200, and 400 mg/kg) significantly inhibited the growth of PbA in Balb/C mice (42.66%, 66.2 1% and 80.99 % inhibition, respectively) (p<0.05). Similarly, all C. costata fractions also produced antimalarial activity against PbA with administration of the ethyl acetate fraction presenting the highest activity (79.85 % inhibition). Conclusion: The C. costata leaves showed antimalarial activity against PbA. However, further studies are necessary to elucidate the underlying mechanisms of this effect and the active compounds involved. Our current study revealed that C.costata could be a potential candidate to be used as a new antimalarial drug.
https://ajp.mums.ac.ir/article_13188_3d30cb9af02cc831d18be06b99e24352.pdf
2019-09-01
474
481
10.22038/ajp.2019.13188
Malaria
Castanopsis costata
Antimalarial drugs
Plasmodium berghei
Maulana
Alkandahri
alkandahri@gmail.com
1
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Padjadjaran University
AUTHOR
Afiat
Berbudi
a.berbudi@unpad.ac.id
2
Department of Biomedical Sciences, Parasitology Division, Faculty of Medicine, Padjadjaran University, Bandung, West Java, Indonesia.
LEAD_AUTHOR
Novi Vicahyani Utami
Utami
3
Department of Biomedical Sciences, Pharmacology Division, Faculty of Medicine, Padjadjaran University, Bandung, West Java, Indonesia.
AUTHOR
Anas
Subarnas
4
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Padjadjaran University, Jatinangor, West Java, Indonesia.
AUTHOR
Alkandahri MY, Nisriadi L, Salim E. 2016. Secondary metabolites and antioxidant activity of methanol extract of Castanopsis costata leaves. Pharmacol Clin Pharm Res, 1: 98-102.
1
Alkandahri MY, Siahaan PN, Salim E, Fatimah C. 2018. Anti inflammatory activity of cep-cepan leaves (Castanopsis costata (Blume) A.DC). Int J Current Med Sci, 8: 424-429.
2
Bantie L, Assefa S, Teklehaimanot T, Engidawork E. 2014. In vivo antimalarial activity of the crude leaf extract and solvent fractions of croton macrostachyus hocsht. (Euphorbiaceae) against Plasmodium berghei in mice. BMC Complement Altern Med, 14: 2-10.
3
Bero J, Quetin-Leclercq J. 2011. Natural products published in 2009 from plants traditionally used to treat malaria. Plant Med, 77: 631-40.
4
Biagini GA, O’Neill, Nzila PM, Ward SA. 2003. Antimalarial chemotherapy: young guns or back to the future. Trends Parasitol, 19: 479-487.
5
Bilia AR, Melilo de Malgalhaes P, Berganzi MC, Vincieri FF. 2006. Simultaneous analysis of artemisinin and flavonoids or several extract of Artimisia annua L. obtained from a Commercial Sample and Selected Cultivar. J Phytomed, 13: 487-493.
6
Builders MI, Wannang NN, Ajoku GA, Builders PF, Orisadipe A, Aguiyi JC. 2010. Evaluation of antimalarial potential of Vernonia ambigua kotschy and peyr (Asteraceae). Int J Pharmacol, 18: 1-9.
7
Burke E, Deasy J, Hasson R, McCormack R, Randhawa V, Walsh P. 2003. Antimalarial drug from nature. J Trinity Student Med: 1-8.
8
Cai S, Risinger AL, Nair S, Peng J, Anderson TJC, Du L, Powell DR, Mooberry SL, Cichewicz RH. 2016. Identification of compounds with efficacy against malaria parasites from common north american plants. J Nat Prod, 79: 490-498.
9
Cooke B, Mohandas N, Coppel R. 2001. The malaria-infected red blood cell: structural and functional changes. Advan Parasitol,50: 1-86.
10
Elfahmi Woerdenbag HJ, Kayser O. 2014. Jamu: Indonesian traditional herbal medicine towards rational phytopharmacological use. J Herb Med 4: 51-73.
11
Fidock DA, Rosenthal PJ, Croft SL, Brun R, Nwaka S. 2004. Antimalarial drug discovery: Efficacy models for compound screening. Nat Rev Drug Disc, 3: 509-520.
12
Frolich S, Schuberth C, Bienzle U, Siems KJ. 2005. In vitro Antiplasmodial activity of prenylated chalcon derivates and their interaction with haemin. J Antimicro Chemother, 55: 883-887.
13
Hayati EK, Jannah A, Ningsih R. 2012. Compounds identification and in vivo antimalarial activity of ethyl acetate extract from anting-anting plant (Acalypha Indica L.). Molekul, 7: 20-32.
14
Hilou A, Nacoulma OG, Guiguemde TR. 2006. In vivo antimalarial activities of extracts Amaranthus spinosus L. and Boerhaavia erecta L. in mice. J Ethnopharmacol, 103: 236-240.
15
Kim K, Lee S, Jung S, Park H, Shinkilt Y, Kim BK. 2003. Antioxidant activities of the extracts from the herbs of Artemisia apiacea. J Ethnopharmacol, 85: 69-72.
16
Kirk, K. 2001. Membran transport in the malaria infected erythrocyte. Physol. Review, 81: 495-537.
17
Kirk, K. 2004. Channels and transporters as drug targets in the plasmodium-infected erythrocyte. J Acta Trop, 89: 285-298.
18
Knight DJ and Peters W. 1980. The antimalarial action of N-benzyloxy dihydrotriazines: The action of cycolguanil (BRL50216) against rodent malaria and studies on its mode of action. Ann Trop Med Parasitol, 74:393-404.
19
Laphookhieo S, Maneerat W, Koysomboon S. 2009. Antimalarial and cytotoxic phenolic compounds from Cratoxylum maingayi and Cratoxylum cochinchinense. Molecule, 14: 1389-1395.
20
Meschnick SR, Taylor TE, Kamchonwongpaisan S. 1996. Artemisinin and the antimalarial endoperoxides: from herbal remedy to targeted chemotherapy. Microbiol Rev, 60: 301-315.
21
Mustofa. 2003. Molekul antimalaria alami: potensi dan tantangan pengembangannya sebagai obat baru untuk malaria. Trad Med J, 8: 8-18.
22
Nogueira CR, Lopes ML. 2011. Antiplasmodial natural products. Molecules, 16: 2146-2190.
23
Nurtjahja K, Kelana TB, Suryanto D, Priyani N, Rio G, Putra DP, Arbain D. 2013. Antimicrobial activity of endemic herbs from tangkahan conservation forest north sumatera to bacteria and yeast. Hayati J Biosci, 20: 177-181.
24
Pan WH, Xu XY, Shi N, Tsang SW, Zhang HJ. 2018. Antimalarial activity of plant metabolites. Int J Mol Sci, 19: 1-40.
25
Pouplin JN, Tran TH, Dolecek C, Phan TA, Farrar J, Carron P, Bodo B, Grellier P. 2007. Antimalarial and cytotoxic activities of ethnopharmacologically selected medicinal plants from south vietnam. J Ethnopharmacol, 109: 417-427.
26
Yadav RNS, Agarwala M. 2011. Phytochemical analysis of some medicinal plants. J Phytol, 3: 10-14.
27
Salim E, Fatimah C, Fanny DY. 2017.
28
Analgetic activity of cep-cepan (Saurauia Cauliflora Dc.) leaves extract. J Nat, 17: 31-38.
29
Saxena S, Pant N, Jain DC, Bhakuni RS. 2003. Antimalarial agent from Plant Source. Curr Sci, 85: 1314-29.
30
ORIGINAL_ARTICLE
Kiwifruit effect on adipose tissue cell size and cholesteryl ester transfer protein gene expression in high-fat diet fed Golden Syrian hamsters
Objective: The effects of kiwifruit on the histology and cell size of adipose tissue in hyperlipidemic models have not yet been reported. Therefore, this study aimed to investigate the effect of kiwifruit on the adipose tissue cell size and activity as well as the gene expression of cholesteryl ester transfer protein (CETP) in high-fat diet (HFD) fed hamsters. Materials and Methods: Forty-two male Syrian hamsters were divided into six groups. Control normal (CN) hamsters received normal diet, control HFD (CHF) were fed with a HFD plus a normal diet (15% butter fat + 0.05% cholesterol + a normal diet). Two groups were fed with normal diet including kiwifruit (1.86; Nd.1 or 3.73 g/kg; Nd.2) and two groups were fed with HFD including kiwifruit (1.86;HFd.1or 3.73 g/kg; HFd.2), for 8 weeks. Results: Histological examination of adipose tissue showed that the cell size was significantly reduced in the kiwifruit-treated groups (low and high dose) in comparison to their control groups (p<0.05). Kiwifruit supplementation (low and high dose) in normal and HFD groups significantly increased gene expression of CETP in adipose tissue. Kiwifruit had no significant effect on serum concentration of low-density lipoprotein cholesterol, total cholesterol and triglyceride. Although, high-density lipoprotein cholesterol concentration increased in HFD-fed hamsters supplemented with 3.73 g/kg of kiwifruit (p<0.05). Conclusion: Kiwifruit consumption reduces the size of adipocytes and increases the expression of CETPgene in adipose tissue cells. Despite the increases in CETP expression in adipose tissue, its activity in serum was not changed following kiwifruit supplementation.
https://ajp.mums.ac.ir/article_13368_97508eec6c07e706b045e08cc9b6b79c.pdf
2019-09-01
482
490
10.22038/ajp.2019.13368
Actinidia
Adipose tissue
Cholesteryl ester transfer protein
High fat diet
Zahra
Zaherijamil
zaherijamilzahra@gmail.com
1
Department of Clinical Biochemistry, Hamadan University of Medical Sciences; Hamadan, Iran
AUTHOR
Narjes
Rezaei
nargesrezaei2011@yahoo.com
2
Students Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
AUTHOR
Mohammad
Hashemnia
m.hashemnia@razi.ac.ir
3
Department of Pathobiology, Faculty of Veterinary Medicine, Razi University, Kermanshah-Iran
AUTHOR
Shirin
Moradkhani
sh.moradkhani@umsha.ac.ir
4
Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
AUTHOR
Massoud
Saidijam
saidijam@umsha.ac.ir
5
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
AUTHOR
Iraj
Khodadadi
khodadadi@umsha.ac.ir
6
Nutrition Health Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
AUTHOR
Ebrahim
Abbasi Oshaghi
7abbasi@gmail.com
7
Department of Clinical Biochemistry, Hamadan University of Medical Sciences; Hamadan, Iran
AUTHOR
Heidar
Tavilani
tavilani@gmail.com
8
Department of Clinical Biochemistry, Hamadan University of Medical Sciences; Hamadan, Iran
LEAD_AUTHOR
Abbasi Oshaghi E, Khodadadi I, Saidijam M, Yadegarazari R, Shabab N, Tavilani H, Goodarzi MT. 2015. Lipid lowering effects of hydroalcoholic extract of anethum graveolens l. and dill tablet in high cholesterol fed hamsters. Cholesterol, 2015:958560.
1
Barter PJ, Brewer HB, Chapman MJ, Hennekens CH, Rader DJ, Tall AR. 2003. Cholesteryl ester transfer protein: a novel target for raising HDL and inhibiting atherosclerosis. Arterioscler Thromb Vasc Biol, 23: 160-167.
2
Brevik A, Gaivão I, Medin T, Jørgenesen A, Piasek A, Elilasson J, Karlsen A, Blomhoff R, Veggan T, Duttaroy AK, Collins AR. 2011. Supplementation of a western diet with golden kiwifruits (Actinidia chinensis var. LHort 16AL:) effects on biomarkers of oxidation damage and antioxidant protection. Nutr J,10: 54.
3
Chang WH, Liu JF. 2009. Effects of kiwifruit consumption on serum lipid profiles and antioxidative status in hyperlipidemic subjects. Int J Food Sci Nutr, 60: 709-716.
4
Gammon CS, Kruger R, Minihane AM, Conlon CA, von Hurst PR, Stonehouse W. 2013. Kiwifruit consumption favourably affects plasma lipids in a randomised controlled trial in hypercholesterolaemic men. Br J Nutr,109: 2208-2218.
5
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