ORIGINAL_ARTICLE
Capsaicin inhibitory effects on Vibrio cholerae toxin genes expression
Objective: Cholera is an acute secretory diarrhea caused by the Gram-negative bacterium, Vibrio cholerae mostly through production of cholera toxin (CT) and zonula occludens toxin (Zot). Isolates of V. cholerae have acquired resistance elements during the last decade. One of the most promising ways to treat resistant strains is to use antivirulence agents instead of killing the causative agent with conventional antibiotics. In this study, we examined whether different concentrations of capsaicin - the pungent fraction of red chili- can act as an antivirulence agent and inhibit V. cholerae toxin production. Materials and Methods: Two standard strains namely, V. cholerae ATCC 14035 and V. cholerae PTCC 1611 were used in this study. Minimum Inhibitory Concentration (MIC) of capsaicin was determined by broth microdilution method. Based on MIC results, the bacteria were cultured in the presence of sub-MIC concentrations of capsaicin and a negative control without capsaicin. Real-time PCR (RT-PCR) was carried out to determine the expression level of V. cholerae toxin genes at each concentration. Results: MIC test showed that 200 mg/mL of capsaicin in 2% dimethyl sulfoxide (DMSO) could inhibit the growth of the two standard strains of V. cholerae. The expression of V. cholerae toxin genes was significantly reduced following treatment with sub-MIC concentrations of capsaicin as assessed by RT-PCR. Conclusion: Capsaicin showed great inhibitory effect against cholera toxin and reduced Zot production in the tested strains of V. cholerae. The results showed promising insights into antivirulence effects of capsaicin.
https://ajp.mums.ac.ir/article_11736_79084788f3829da7e04045d60fee065f.pdf
2019-05-01
187
194
10.22038/ajp.2018.11736
Vibrio cholera
Antivirulence agents
Capsaicin
Toxin gene expression
Soroor
Erfanimanesh
s_erfanimanesh@yahoo.com
1
Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
gita
eslami
g_eslami@yahoo.com
2
Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
Arezou
Taherpour
are_taherpour@yahoo.com
3
Department of Microbiology, Kurdistan University of Medical Sciences, Sanandaj, Iran
AUTHOR
Ali
Hashemi
hashemi1388@yahoo.com
4
Depratment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Ahmad B, Rehman MU, Amin I, Arif A, Rasool S, Bhat SA, Afzal I, Hussain I, Bilal S. 2015. A review on pharmacological properties of zingerone (4-(4-Hydroxy-3-methoxyphenyl)-2-butanone). Sci World J, 2015: 816364.
1
Allen RC, Popat R, Diggle SP, Brown SP. 2014. Targeting virulence: can we make evolution-proof drugs? Nat Rev Microbiol, 12: 300-308.
2
Anthouard R, DiRita VJ. 2013. Small-molecule inhibitors of toxT expression in Vibrio cholerae. MBio, 4: e00403-13.
3
Chatterjee S, Asakura M, Chowdhury N, Neogi SB, Sugimoto N, Haldar S, Awasthi SP, Hinenoya A, Aoki S, Yamasaki S. 2010. Capsaicin, a potential inhibitor of cholera toxin production in Vibrio cholerae. FEMS Microbiol Lett, 306: 54-60.
4
Clatworthy AE, Pierson E, Hung DT. 2007. Targeting virulence: a new paradigm for antimicrobial therapy. Nat Chem Biol, 3:541-548.
5
Clinical and Laboratory Standards Institute (CLSI): Performance standards for antimicrobial susceptibility testing. 2012. Twenty-second informational supplement. Document M100-S22 Wayne PC.
6
Fasano A, Fiorentini C, Donelli G, Uzzau S, Kaper JB, Margaretten K, Ding XU, Guandalini S, Comstock L, Goldblum SE. 1995. Zonula occludens toxin modulates tight junctions through protein kinase C-dependent actin reorganization, in vitro. J Clin Invest, 96: 710-720.
7
Harris JB, LaRocque RC, Qadri F, Ryan ET, Calderwood SB. 2012. Cholera. Lancet (London, England), 30: 2466-2476.
8
Jensen PG, Curtis PD, Dunn JA, Austic RE, Richmond ME. 2003. Field evaluation of capsaicin as a rodent aversion agent for poultry feed. Pest Manag Sci, 59: 1007-1015.
9
Kalia NP, Mahajan P, Mehra R, Nargotra A, Sharma JP, Koul S, Khan IA. 2012. Capsaicin, a novel inhibitor of the NorA efflux pump, reduces the intracellular invasion of Staphylococcus aureus. J Antimicrob Chemother, 67: 2401-2408.
10
Kitaoka M, Miyata ST, Unterweger D, Pukatzki S. 2011. Antibiotic resistance mechanisms of Vibrio cholerae. J Med Microbiol, 60: 397-407.
11
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12
Laxminarayan R, Duse A, Wattal C, Zaidi AK, Wertheim HF, Sumpradit N, Vlieghe E, Hara GL, Gould IM, Goossens H, Greko C, So AD, Bigdeli M, Tomson G, Woodhouse W, Ombaka E, Peralta AQ, Qamar FN, Mir F, Kariuki S, Bhutta ZA, Coates A, Bergstrom R, Wright GD, Brown ED, Cars O. 2013. Antibiotic resistance-the need for global solutions. Lancet Infect Dis, 13:1057-1098.
13
Marini E, Magi G, Mingoia M, Pugnaloni A, Facinelli B. 2015. Antimicrobial and anti-virulence activity of capsaicin against erythromycin-resistant, cell-invasive group a streptococci. Front Microbiol, 6:1281-1288.
14
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15
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16
Muanprasat C, Chatsudthipong V. 2013. Cholera: pathophysiology and emerging therapeutic targets. Future med chem, 5: 781-798.
17
Nataro JP, Kaper JB. Diarrheagenic escherichia coli. 1998. Clin Microbiol Rev, 11:142-201.
18
Rasko DA, Sperandio V. 2010. Anti-virulence strategies to combat bacteria-mediated disease. Nat Rev Drug Discov, 9: 117-128.
19
Roca I, Akova M, Baquero F, Carlet J, Cavaleri M, Coenen S, Cohen J, Findlay D, Gyssens I, Heure OE, Kahlmeter G. 2015. The global threat of antimicrobial resistance: science for intervention. New Microbes New Infect, 6: 22-29.
20
Sack DA. Sack RB, Nair GB, and Siddique AK. 2004. Cholera. Lancet, 363:223-233.
21
Shakibaie MR, Shahcheraghi F, Hashemi A, Adeli NS. 2008. Detection of TEM, SHV and PER Type Extended-Spectrum ß-Lactamase Genes among Clinical Strains of Pseudomonas aeruginosa Isolated from Burnt Patients at Shafa-Hospital, Kerman, Iran. Iran J Basic MedSci, 11: 104-111.
22
Silver LL. 2011. Challenges of antibacterial discovery. Clin Microbiol Rev, 24: 71-109.
23
Srinivasan K. 2016. Biological activities of red pepper (Capsicum annuum) and its pungent principle capsaicin: a review. Crit Rev Food Sci Nutr, 56:1488-1500.
24
Sumitomo T, Nakata M, Yamaguchi M, Terao Y, Kawabata S. 2012. S-carboxymethylcysteine inhibits adherence of Streptococcus pneumoniae to human alveolar epithelial cells. J Med Microbiol, 61: 101-108.
25
Trærup SL, Ortiz RA, Markandya A. 2011. The costs of climate change: a study of cholera in Tanzania. Int J Environ Res Public Health, 8: 4386-4405.
26
Zahid MS, Awasthi SP, Asakura M, Chatterjee S, Hinenoya A, Faruque SM, Yamasaki S. 2015. Suppression of virulence of toxigenic Vibrio cholerae by anethole through the cyclic AMP (cAMP)-cAMP receptor protein signaling system. PloS one, 10: e0137529.
27
Zhang S, Ma X, Zhang L, Sun H, Liu X. 2017. Capsaicin reduces blood glucose by increasing insulin levels and glycogen content better than capsiate in streptozotocin-induced diabetic rats. J Agric Food Chem, 65: 2323-2330.
28
Zhong Z, Yu X, Zhu J. 2008. Red bayberry extract inhibits growth and virulence gene expression of the human pathogen Vibrio cholerae. J Antimicrob Chemother, 61: 753-754.
29
ORIGINAL_ARTICLE
Clinical and experimental effects of Nigella sativa and its constituents on respiratory and allergic disorders
Objective: Black cumin or Nigella sativa (N. sativa) seed has been widely used traditionally as a medicinal natural product because of its therapeutic effects. In this review, the medicinal properties of N. sativa as a healing remedy for the treatment of respiratory and allergic diseases, were evaluated. Material and Methods: Keywords including Nigella sativa, black seed, thymoquinone, respiratory, pulmonary, lung and allergic diseases were searched in medical and nonmedical databases (i.e. PubMed, Science Direct, Scopus, and Google Scholar). Preclinical studies and clinical trials published between 1993 and 2018 were selected. Results: In experimental and clinical studies, antioxidant, immunomodulatory, anti-inflammatory, antihistaminic, antiallergic, antitussive and bronchodilatory properties of N. sativa different extracts, extracts fractions and constituents were demonstrated. Clinical studies also showed bronchodilatory and preventive properties of the plant in asthmatic patients. The extract of N. sativa showed a preventive effect on lung disorders caused by sulfur mustard exposure. The therapeutic effects of the plant and its constituents on various allergic disorders were also demonstrated. Conclusion: Therefore, N. sativa and its constituents may be considered effective remedies for treatment of allergic and obstructive lung diseases as well as other respiratory diseases.
https://ajp.mums.ac.ir/article_12196_792541697ef14eb490fa54af5aad07a2.pdf
2019-05-01
195
212
10.22038/ajp.2019.12196
Nigella Sativa
Thymoquinone
Experimental effect
Clinical effect
Respiratory diseases
Allergic diseases
Zahra
Gholamnezhad
gholamnezhadz@mums.ac.ir
1
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
Farzaneh
Shakeri
f_1366_sh@yahoo.com
2
Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
AUTHOR
Saiedeh
Saadat
saadats931@mums.ac.ir
3
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Vahideh
Ghorani
ghoranisv921@mums.ac.ir
4
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mohammad Hossein
Boskabady
boskabadymh@mums.ac.ir
5
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
LEAD_AUTHOR
Abbas AT, Abdel-Aziz MM, Zalata K, Abd T-DA-G. 2005. Effect of dexamethasone and Nigella sativa on peripheral blood eosinophil count, IgG1 and IgG2a, cytokine profiles and lung inflammation in murine model of allergic asthma. Egypt J Immunol, 12:95-102.
1
Abidi A, Robbe A, Kourda N, Ben Khamsa S, Legrand A. 2017. Nigella sativa, a traditional Tunisian herbal medicine, attenuates bleomycin-induced pulmonary fibrosis in a rat model. Biomed Pharmacother, 90:626-637.
2
Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Damanhouri ZA, Anwar F. 2013. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed, 3:337-352.
3
Ahmad J, Khan RA, Malik MA. 2010. A study of Nigella sativa oil in the management of wheeze associated lower respiratory tract illness in children. Afr J Pharm Pharmacol, 4:436-439.
4
Al-Jassir MS. 1992. Chemical composition and microflora of black cumin (Nigella sativa L.) seeds growing in Saudi Arabia. Food Chem, 45:239-242.
5
Al-Jawad FH, Al-Razzuqi RA, Hashim HM, Ismael AH. 2012. Broncho-relaxant activity of Nigella sativa versus anthemisnobilis in chronic bronchial asthma; a comparative study of efficacy. IOSR J Pharmac, 2:81-83.
6
Al-Majed AA, Daba MH, Asiri YA, Al-Shabanah OA, Mostafa AA, El-Kashef HA. 2001. Thymoquinone-induced relaxation of guinea-pig isolated trachea. Res Commun Mol Pathol Pharmacol, 110:333-345.
7
Al-Sheddi ES, Farshori NN, Al-Oqail MM, Musarrat J, Al-Khedhairy AA, Siddiqui MA. 2014. Cytotoxicity of Nigella sativa seed oil and extract against human lung cancer cell line. Asian Pac J Cancer Prev, 15:983-987.
8
Ali BH, Blunden G. 2003. Pharmacological and toxicological properties of Nigella sativa. Phytother Res, 17:299-305.
9
Alsamarai AM, Abdulsatar M, Ahmed Alobaidi AH. 2014. Evaluation of topical black seed oil in the treatment of allergic rhinitis. Antiinflamm Antiallergy Agents Med Chem, 13:75-82.
10
Ameen NMA, Altubaigy F, Jahangir T, Mahday IA, Mohammed EA, Musa OAA. 2011. Effect of Nigella sativa and bee honey on pulmonary, hepatic and renal function in Sudanese in Khartoum state. J Med Plant Res, 5:6857-6863.
11
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12
Ansari MA, Ansari NA, Junejo SA. 2010. Montelukast versus nigella sativa for management of seasonal allergic rhinitis: A single blind comparative clinical trial. Pak J Med Sci, 26:249-254.
13
Attoub S, Sperandio O, Raza H, Arafat K, Al-Salam S, Al Sultan MA, Al Safi M, Takahashi T, Adem A. 2013. Thymoquinone as an anticancer agent: evidence from inhibition of cancer cells viability and invasion in vitro and tumor growth in vivo. Fundam Clin Pharmacol, 27:557-569.
14
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15
Balaha MF, Tanaka H, Yamashita H, Rahman MNA, Inagaki N. 2012. Oral Nigella sativa oil ameliorates ovalbumin-induced bronchial asthma in mice. Int Immunopharmacol, 14:224-231.
16
Balkissoon R, Lommatzsch S, Carolan B, Make B. 2011. Chronic obstructive pulmonary disease: a concise review. Med Clin North Am, 95:1125-1141.
17
Bayir Y, Albayrak A, Can I, Karagoz Y, Cakir A, Suleyman H, Uyanik H, Yayla N, Polat B, Karakus E, Keles MS. 2012. Nigella sativa as a potential therapy for the treatment of lung injury caused by cecal ligation and puncture-induced sepsis model in rats. Cell Mol Bio), 58 Suppl:OL1680-1687.
18
Boskabady H, Keyhanmanesh R, Saadatloo MA. 2008. Relaxant effects of different fractions from Nigella sativa L. on guinea pig tracheal chains and its possible mechanism (s). Indian J Exp Biol, 46:805-810.
19
Boskabady M, Mohsenpoor N, Takaloo L. 2010. Antiasthmatic effect of Nigella sativa in airways of asthmatic patients. Phytomedicine, 17:707-713.
20
Boskabady MH, Javan H, Sajady M, Rakhshandeh H. 2007. The possible prophylactic effect of Nigella sativa seed extract in asthmatic patients. Fundam Clin Pharmacol, 21:559-566.
21
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22
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23
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26
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27
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28
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29
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33
Fallahi M, Keyhanmanesh R, Khamaneh AM, Saadatlou MAE, Saadat S, Ebrahimi H. 2016. Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats. Avicenna J Phytomed, 6:77-85.
34
Gholamnezhad Z, Boskabady MH, Hosseini M. 2014. Effect of Nigella sativa on immune response in treadmill exercised rat. BMC Complement Altern Med, 14:437.
35
Gholamnezhad Z, Havakhah S, Boskabady MH. 2016. Preclinical and clinical effects of Nigella sativa and its constituent, thymoquinone: A review. J Ethnopharmacol, 190:372-386.
36
Gholamnezhad Z, Keyhanmanesh R, Boskabady MH. 2015a. Anti-inflammatory, antioxidant, and immunomodulatory aspects of Nigella sativa for its preventive and bronchodilatory effects on obstructive respiratory diseases: A review of basic and clinical evidence. J Funct Foods, 17:910-927.
37
Gholamnezhad Z, Rafatpanah H, Sadeghnia HR, Boskabady MH. 2015b. Immunomodulatory and cytotoxic effects of Nigella sativa and thymoquinone on rat splenocytes. Food Chem Toxicol, 86:72-80.
38
Gilani A, Aziz N, Khurram I, Chaudhary K, Iqbal A. 2001. Bronchodilator, spasmolytic and calcium antagonist activities of Nigella sativa seeds (Kalonji): a traditional herbal product with multiple medicinal uses. J Pak Med Assoc, 51:115-120.
39
Günel C, Demirci B, Meteoğlu İ, Yılmaz M, Ömürlü İK, Kocatürk T. 2017. The anti-inflammatory effects of thymoquinone in a rat model of allergic rhinitis. J Ear Nose Throat, 27:226-232.
40
Gunes AE, Gozeneli O, Akal AA, Guldur ME, Savik E. 2017. Reduction of side effects of hyperbaric oxygen therapy with thymoquinone treatment in rats. Undersea Hyperb Med, 44:337-343.
41
Hossein BM, Nasim V, Sediqa A. 2008. The protective effect of Nigella sativa on lung injury of sulfur mustard-exposed Guinea pigs. Exp Lung Res, 34:183-194.
42
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43
Işık H, Çevikbaş A, Gürer ÜS, Kıran B, Üresin Y, Rayaman P, Rayaman E, Gürbüz B, Büyüköztürk S. 2010. Potential adjuvant effects of Nigella sativa seeds to improve specific immunotherapy in allergic rhinitis patients. Med Princ Pract, 19:206-211.
44
Jafri SH, Glass J, Shi R, Zhang S, Prince M, Kleiner-Hancock H. 2010. Thymoquinone and cisplatin as a therapeutic combination in lung cancer: In vitro and in vivo. J Exp Clin Cancer Res, 29:87.
45
Kacem R, Meraihi Z. 2006. Effects of essential oil extracted from Nigella sativa (L.) seeds and its main components on human neutrophil elastase activity. Yakugaku Zasshi, 126:301-305.
46
Kalus U, Pruss A, Bystron J, Jurecka M, Smekalova A, Lichius JJ, Kiesewetter H. 2003. Effect of Nigella sativa (black seed) on subjective feeling in patients with allergic diseases. Phytothe Res, 17:1209-1214.
47
Kanter M. 2009. Effects of Nigella sativa seed extract on ameliorating lung tissue damage in rats after experimental pulmonary aspirations. Acta Histochem, 111:393-403.
48
Kanter M. 2011. Thymoquinone attenuates lung injury induced by chronic toluene exposure in rats. Toxicol Ind Health, 27:387-395.
49
Kardani AK, Fitri LE, Barlianto W, Olivianto E, Kusuma C. 2013. The effect of house dust mite immunotherapy, probiotic and Nigella sativa in the number of Th17 cell and asthma control test score. IOSR J Dent Med Sci, 6:37-47.
50
Keyhanmanesh R, Bagban H, Nazemieh H, Bavil FM, Alipour MR. 2013. The main relaxant constituents of Nigella sativa methanolic fraction on Guinea pig tracheal chains. Iran J Allergy Asthma Immunol, 12:136-143.
51
Keyhanmanesh R, Gholamnezhad Z, Boskabady MH. 2014a. The relaxant effect of Nigella sativa on smooth muscles, its possible mechanisms and clinical applications. Iran J Basic Med Sci, 17:939-949.
52
Keyhanmanesh R, Nazemiyeh H, Mazouchian H, Asl MMB, Shoar MK, Alipour MR, Boskabady MH. 2014b. Nigella sativa pretreatment in guinea pigs exposed to cigarette smoke modulates in vitro tracheal responsiveness. Iran Red Crescent Med J, 16: e10421.
53
Keyhanmanesh R, Pejman L, Omrani H, Mirzamohammadi Z, Shahbazfar AA. 2014c. The effect of single dose of thymoquinone, the main constituents of Nigella sativa, in guinea pig model of asthma. BioImpacts, 4:75-81.
54
Keyhanmanesh R, Saadat S, Mohammadi M, Shahbazfar AA, Fallahi M. 2015. The Protective Effect of α‐Hederin, the Active Constituent of Nigella sativa, on Lung Inflammation and Blood Cytokines in Ovalbumin Sensitized Guinea Pigs. Phytother Res, 29:1761-1767.
55
Koshak A, Wei L, Koshak E, Wali S, Alamoudi O, Demerdash A, Qutub M, Pushparaj PN, Heinrich M. 2017. Nigella sativa supplementation improves asthma control and biomarkers: A randomized, double‐blind, placebo‐controlled trial. Phytother Res, 31:403-409.
56
Mansour M, Tornhamre S. 2004. Inhibition of 5-lipoxygenase and leukotriene C4 synthase in human blood cells by thymoquinone. J Enzyme Inhib Med Chem, 19:431-436.
57
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59
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60
Norouzi F, Hosseini M, Abareshi A, Beheshti F, Khazaei M, Shafei MN, Soukhtanloo M, Gholamnezhad Z, Anaeigoudari A. 2018. Memory enhancing effect of Nigella Sativa hydro-alcoholic extract on lipopolysaccharide-induced memory impairment in rats. Drug Chem Toxicol, 28:1-10.
61
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62
Pourgholamhossein F, Sharififar F, Rasooli R, Pourgholi L, Nakhaeipour F, Samareh-Fekri H, Iranpour M, Mandegary A. 2016. Thymoquinone effectively alleviates lung fibrosis induced by paraquat herbicide through down-regulation of pro-fibrotic genes and inhibition of oxidative stress. Environ Toxicol Pharmacol, 45:340-345.
63
Rooney S, Ryan MF. 2005. Effects of alpha-hederin and thymoquinone, constituents of Nigella sativa, on human cancer cell lines. Anticancer Res, 25:2199-2204.
64
Saadat S, Mohammadi M, Fallahi M, Aslani MR. 2015. The protective effect of α-hederin, the active constituent of Nigella sativa, on tracheal responsiveness and lung inflammation in ovalbumin-sensitized guinea pigs. J Physiol Sci, 65:285-292.
65
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68
Shakeri F, Gholamnezhad Z, Mégarbane B, Rezaee R, Boskabady MH. 2016. Gastrointestinal effects of Nigella sativa and its main constituent, thymoquinone: a review. Avicenna J Phytomed, 6:9-20.
69
Suddek GM, Ashry NA, Gameil NM. 2013. Thymoquinone attenuates cyclophosphamide-induced pulmonary injury in rats. Inflammopharmacology, 21:427-435.
70
Susanti N, Barlianto W, Kalim H, Kusuma HC. 2013. Asthma clinical improvement and reduction in the number of CD4 CD25 foxp3 Treg and CD4 IL-10 cells after administration of immunotherapy house dust mite and adjuvant probiotics and/or Nigella Sativa powder in mild asthmatic children. IOSR J Dent Med Sci, 7:50-59.
71
Tavakkoli A, Mahdian V, Razavi BM, Hosseinzadeh H. 2017. Review on clinical trials of black seed (Nigella sativa ) and its active constituent, thymoquinone. J Pharmacopuncture, 20:179-193.
72
Tayman C, Cekmez F, Kafa IM, Canpolat FE, Cetinkaya M, Tonbul A, Uysal S, Tunc T, Sarici SU. 2013. Protective effects of Nigella sativa oil in hyperoxia-induced lung injury. Arch Bronconeumol, 49:15-21.
73
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74
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75
Wienkotter N, Hopner D, Schutte U, Bauer K, Begrow F, El-Dakhakhny M, Verspohl EJ. 2008. The effect of nigellone and thymoquinone on inhibiting trachea contraction and mucociliary clearance. Planta Med, 74:105-108.
76
Womack K, Anderson M, Tucci M, Hamadain E, Benghuzzi H. 2006. Evaluation of bioflavonoids as potential chemotherapeutic agents. Biomed Sci Instrum, 42:464-469.
77
Woo CC, Kumar AP, Sethi G, Tan KH. 2012. Thymoquinone: potential cure for inflammatory disorders and cancer. Biochem Pharmacol, 83:443-451.
78
Yang J, Kuang XR, Lv PT, Yan XX. 2015. Thymoquinone inhibits proliferation and invasion of human nonsmall-cell lung cancer cells via ERK pathway. Tumour Biol, 36:259-269.
79
Yoruk O, Tatar A, Keles ON, Cakir A. 2017. The value of Nigella sativa in the treatment of experimentally induced rhinosinusitis. Acta Otorhinolaryngol Ital, 37:32-37.
80
Zhu N, Zhao X, Xiang Y, Ye S, Huang J, Hu W, Lv L, Zeng C. 2016. Thymoquinone attenuates monocrotaline-induced pulmonary artery hypertension via inhibiting pulmonary arterial remodeling in rats. Int J Cardiol, 221:587-596.
81
ORIGINAL_ARTICLE
Protective effect of Berberis vulgaris on Fenton reaction-induced DNA cleavage
Objective: Berberis vulgaris contains antioxidants that can inhibit DNA cleavage. The purpose of this study was to evaluate the antioxidant and protective activity of B. vulgaris on DNA cleavage. Materials and Methods: In this study, the antioxidant capacity of B. vulgaris was investigated using DPPH and its protective effect was evaluated on pBR322 plasmid and lymphocyte genomic DNA cleavage induced by Fenton reaction, by DNA electrophoresis. Results: Aqueous extract of B. vulgaris presented dual behavior with a potent antioxidant activity at 0.25and 0.75mg/ml for pBR322 plasmid and lymphocyte genomic DNA, respectively, but a pro-oxidant activity was observed at higher concentrations. Conclusion: Our results indicated that B. vulgaris extract an inhibit Fenton reaction-induced DNA cleavage and oxidative cleavage of double-stranded DNA assay is a powerful technique that can be used to determine the antioxidant and pro-oxidant properties of a compound on cellular components such as DNA.
https://ajp.mums.ac.ir/article_11788_a4f9d93a31be7b2a8495495ea302f212.pdf
2019-05-01
213
220
10.22038/ajp.2018.11788
Antioxidant activity
Berberis vulgaris
DNA damage
Fenton reaction
Nooshin Sadat
Asadi
mehrnush_asadi@yahoo.com
1
Department of Biology, Faculty of Science, Yazd University, Yazd, Iran
AUTHOR
Mohammad Mehdi
Heidari
heidarimm@yazd.ac.ir
2
Department of Biology, Faculty of Science, Yazd University, Yazd, Iran
LEAD_AUTHOR
Mehri
Khatami
m.khatami@yazd.ac.ir
3
Department of Biology, Faculty of Science, Yazd University, Yazd, Iran
AUTHOR
Abbas SR, Sabir SM, Ahmad SD, Boligon AA, Athayde ML. 2014. Phenolic profile, antioxidant potential and DNA damage protecting activity of sugarcane (Saccharum officinarum). Food Chem, 147: 10-16.
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6
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31
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32
ORIGINAL_ARTICLE
Anti-inflammatory and anti-neuropathic effects of a novel quinic acid derivative from Acanthus syriacus
Objective: Acanthus syriacus (AS) is one of the valuable herbal plants with immunomodulatory potentials. The aim of this study is to assemble a phytochemical investigation of A. syriacus exploring its anti-inflammatory and antinociceptive properties, identification of its most active compound(s) and elucidating their structure and determining their mechanisms of action. Materials and Methods: Bio-guided fractionation and isolation-schemes were used utilizing RP-HPLC, CC, 1H- and 13C-NMR, and biological-models were used to evaluate their effects against inflammation and neuropathic-pain (NP). Results: The outcomes showed that the most active fraction (FKCA) of AS was identified. Two of the three components of FKCA were identified by chromatographic-methods, while the third compound was isolated, its structure was elucidated and its was named Kromeic acid (KRA); FKCA contained Ferulic acid (27.5%), kromeic acid (48.1%), and chlorogenic acid (24.4%). AS, FKCA and KRA showed significant (p˂0.05) anti-inflammatory and antinociceptive potentials in the management of allodynia and thermal-hyperalgesia in NP. AS and FCKA showed comparatively equipotent antinociceptive-effects. FKCA showed higher antinociceptive effects than KRA suggesting additive-effects among FKCA components. The anti-inflammatory, insulin secretagogue, oxidative-stress reducing, and protective effects against NO-induced neuronal-toxicity might be amongst the possible mechanisms of tested compounds to alleviate NP. Conclusion: Here, we report the isolation and structure elucidation of a novel quinic-acid derivative, KRA. A. syriacus, FKCA, and KRA might be used as a novel complementary approach to ameliorate a variety of painful-syndromes.
https://ajp.mums.ac.ir/article_12218_e7dc083115e0386fcae385ccf716bad7.pdf
2019-05-01
221
236
10.22038/ajp.2019.12218
Novel quinic acid derivative
Acanthus syriacus
Anti-inflammatory
antinociceptive effects
Kromeic acid
Karim
Raafat
k.raafat@bau.edu.lb
1
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Beirut Arab University, 115020 Beirut, Lebanon
LEAD_AUTHOR
Asongalem EA, Foyet HS, Ekobo S, Dimo T, Kamtchouing P. 2004. Antiinflammatory, lack of central analgesia and antipyretic properties of Acanthus montanus (Ness) T. Anderson. J Ethnopharmacol, 95:63-68.
1
Babu BH, Shylesh BS, Padikkala J. 2001. Antioxidant and hepatoprotective effect of Acanthus ilicifolius. Fitoterapia, 72:272-277.
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24
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25
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26
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27
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28
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30
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37
ORIGINAL_ARTICLE
Interactive anticancer effect of nanomicellar curcumin and galbanic acid combination therapy with some common chemotherapeutics in colon carcinoma cells
Objective: In the current investigation, we aimed to study the combined cytotoxicity of curcumin, as a nanomicellar formulation, and galbanic acid (Gal), dissolved in DMSO against the murine C26 and human Caco-2 colon carcinoma cells. Further, curcumin potential for cisplatin and doxorubicin (Dox) co-therapy was studied. Materials and Methods: The combined cytotoxic effect of these phytochemicals at varying dose ratios were examined using the MTT colorimetric assay. Moreover, the time-dependent toxicity of curcumin, cisplatin, Dox, and pegylated liposomal Dox (Doxil) was determined. The interactive anti-proliferative behavior of these compounds was examined using the CompuSyn software. Results: Nanomicellar curcumin showed considerable cytotoxicity in C26 cells 24 hr post-treatment. Co-treatment of cells with curcumin nanomicelles: Gal had a synergistic effect in C26 (at 10:1 molar ratio), and Caco-2 (at 1:5 molar ratio) cell lines in cell cultures. Nanomicellar curcumin showed strong and mild synergistic inhibitory effects in C26 cells when co-administered with Doxil and cisplatin, respectively. Conclusion: Curcumin nanomicelles and Gal had a synergistic effect in C26 and Caco-2 cell lines. It is speculated that nanomicellar curcumin shows synergistic cancer cell killing if administered 24-hr post-injection of Doxil and cisplatin.
https://ajp.mums.ac.ir/article_12265_6faf6229bbbccc14d39a04a7403c7a24.pdf
2019-05-01
237
247
10.22038/ajp.2019.12265
Nanomicellar curcumin
Galbanic acid
Doxil
Cisplatin
Combination therapy
Synergism
Arash
Jafari
arashmd@gmail.com
1
School of Medicine, Birjand University of Medical Sciences, Birjand, Iran
AUTHOR
Manouchehr
Teymouri
manochehr.teimory@gmail.com
2
Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.
AUTHOR
Maryam
Ebrahimi Nik
ebrahiminm921@mums.ac.ir
3
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Azam
Abbasi
abbasia2@mums.ac.ir
4
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mehrdad
Iranshahi
iranshahim@mums.ac.ir
5
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mohammad Yahya
Hanafi-Bojd
my-hanafi_bojd@yahoo.com
6
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
LEAD_AUTHOR
Mahmoud Reza
Jafari
jafarimr@mums.ac.ir
7
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
LEAD_AUTHOR
Ahmadi F, Shokoohinia Y, Javaheri S, Azizian H. 2017. Proposed binding mechanism of galbanic acid extracted from Ferula assa-foetida to DNA.J Photochem Photobiol B, 166: 63-73.
1
Ahmadi M, Agah E, Nafissi S, Jaafari MR, Harirchian MH, Sarraf P, Faghihi-Kashani S, Hosseini SJ, Ghoreishi A, Aghamollaii V, Hosseini M, Tafakhori A. 2018. Safety and efficacy of nanocurcumin as add-on therapy to riluzole in patients with amyotrophic lateral sclerosis: a pilot randomized clinical trial. Neurotherapeutics, 152: 430-438.
2
Barenholz Y. 2012. Doxil®-the first FDA-approved nano-drug: lessons learned. J Control Release, 1602: 117-134.
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6
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7
Hanafi-Bojd MY, Jaafari MR, Ramezanian N, Xue M, Amin M, Shahtahmassebi, Malaekeh-Nikouei B. 2015. Surface functionalized mesoporous silica nanoparticles as an effective carrier for epirubicin delivery to cancer cells. Eur J Pharm Biopharm, 89: 248-258.
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Hu CM, Zhang L. 2012. Nanoparticle-based combination therapy toward overcoming drug resistance in cancer. Biochem Pharmacol, 838: 1104-1111.
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Kasaian J, Iranshahy M, Iranshahi M. 2013. Synthesis, biosynthesis and biological activities of galbanic acid - A review. Pharm Biol, 52: 524-531
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Kasaian J, Mosaffa F, Behravan J, Masullo M, Piacente S, Ghandadi M, Iranshahi M. 2015. Reversal of P-glycoprotein-mediated multidrug resistance in MCF-7/Adr cancer cells by sesquiterpene coumarins. Fitoterapia, 103: 149-154.
12
Kim KH, Lee HJ, Jeong SJ, Lee HJ, Lee EO, Kim HS, Zhang Y, Ryu SY, Lee MH, Lu J, Kim SH. 2011. Galbanic acid isolated from Ferula assafoetida exerts in vivo anti-tumor activity in association with anti-angiogenesis and anti-proliferation. Pharm Res, 283: 597-609.
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Oh BS, Shin EA, Jung JH, Jung DB, Kim B, Shim BS, Yazdi MC, Iranshahi M, Kim SH. 2015. Apoptotic effect of galbanic acid via activation of caspases and inhibition of Mcl‐1 in H460 non‐small lung carcinoma cells. Phytother Res, 296: 844-849.
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18
Teymouri M, Barati N, Pirro M, Sahebkar A. 2018. Biological and pharmacological evaluation of dimethoxycurcumin: A metabolically stable curcumin analogue with a promising therapeutic potential. J Cell Physiol, 2331: 124-140.
19
Teymouri M, Pirro M, Johnston TP, Sahebkar A. 2017. Curcumin as a multifaceted compound against human papilloma virus infection and cervical cancers: A review of chemistry, cellular, molecular, and preclinical features. BioFactors, 433: 331-346.
20
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21
ORIGINAL_ARTICLE
Evaluation of the protective and therapeutic effects of Pistacia atlantica gum aqueous extract on cellular and pathological aspects of experimental asthma in Balb/c mice
Objective: The purpose of this study was to investigate the protective and therapeutic effects of aqueous extract of P. atlantica gum on an experimental asthma in BALB/c mice. Materials and Methods: Aqueous extract of dried and milled P. atlantica gum was assemble andevaluate by GC-MS. In order to investigate the effect of P. atlantica gum extract on cellular and pathological aspects of asthma, 60 BALB/c mice were divided into six groups as: negative control, asthmatic group, asthmatic group receiving dexamethasone (1mg/kg; intraperitoneal (IP)) and three asthmatic groups receiving different concentrations of the extract (100, 200 and 400 mg/kg, orally) from the beginning of the study and continued for 84 days. The examined parameters included cell population, IgE antibody production, levels of IL-4, IL-5, TGF-β, INF-γ, IL-10, and IL-17 cytokines, and lung tissue damage. Results: Regardless of the dose, aqueous extract of P. atlantica gum, caused significant decrease in the number of BALF eosinophilic cells and levels of anti-ovalbumin IgE, IL-4, IL-5 and IL-17 cytokine levels, as well as pathologic damage of the lung tissue. In addition, the amount of anti-inflammatory IL-10, TGF-β, and INF-γ Th1 cytokines significantly increased in the extract-treated groups compared to the asthmatic and dexamethasone-treated groups. Moreover, IFN-γ/IL-4 ratio significantly increased in a dose-dependent manner compared to the un-treated asthma group. Conclusion: The aqueous extract of P. atlantica gum can be considered as a potent anti-inflammatory and immunomodulatory compound and may be used as a natural compound for treatment of immune system disorders.
https://ajp.mums.ac.ir/article_12310_e895ddbc373ff8ca357b7be938a891bc.pdf
2019-05-01
248
259
10.22038/ajp.2019.12310
Asthma
P. atlantica
Balb/C mouse
Zaynab
Shakrami
shakarami_60@yahoo.com
1
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
hadi
Esmaeili Gouvarchin ghaleh
h.smaili1369@gmail.com
2
Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
AUTHOR
bahman
mansouri motlagh
b.mansori68@gmail.com
3
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
Ali
Sheikhian
alisheikhian@gmail.com
4
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences.
LEAD_AUTHOR
Bahman
Jalali Kondori
bahmanjalali2010@gmail.com
5
Faculty of medicine, Baqiyatallah University of medical sciences, Tehran, Iran
AUTHOR
Abediankenari S, Ghasemi M. 2009. Generation of immune inhibitory dendritic cells and CD4+T regulatory cells inducing by TGF beta. Iran J Allergy Asthma Immunol, 8:25-30.
1
Abtahi Froushani SM, Esmaili Gourvarchin Galeh H. 2014. New insight into the immunomodulatory mechanisms of Tretinoin in NMRI mice. Iran J Basic Med Sci, 17:632-637.
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Abtahi Froushani SM, Esmaili gouvarchin Galee H, Khamisabadi M, Lotfallahzade B. 2015. Immunomudulatory effects of hydroalcoholic extract of Hypericum perforatum. Avicenna J Phytomed, 5: 62-68.
3
Abtahi Froushani SM, Zarei L, Esmaeili Gouvarchin Ghaleh H, Mansori Motlagh B.2016. Estragole and methyleugenol-free extract of Artemisia dracunculus possess immunomodulatory effects. Avicenna J Phytomed, 6: 526-534.
4
Ashokkumar K, Selvaraj K, Muthukrishnan SD. 2013. Cynodon dactylon (L.) Pers.: An updated review of its phytochemistry and pharmacology. J Med Plants Res, 7:3477–3483.
5
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6
Bahrami Mahne S, Mahdaviani SA. 2014. Role of the immune cells, mediators and cytokines in the pathogenesis of asthma: a review article. Tehran Univ Med J, 72:273-285.
7
Chuanfeng F, Xiaoxia L, Hongyan L, Li X, Lei L, Chun Y, Guangqiang G, Xiaofeng J. 2015. Downregulation of SUMF2 gene in the ovalbumin-induced rat model of allergic inflammation. Int J Clin Exp Pathol, 8:12053-12063.
8
Esmaili Gourvarchin Galeh H, Abtahi Froushani SM, Afzale Ahangaran N, Naji Hadai S. 2018. Effects of educated monocytes with xenogeneic mesenchymal stem cell–derived conditioned medium in a mouse model of chronic asthma. Immunol Invest, 47: 504-520.
9
Finotto S, Eigenbrod T, Karwot R, Boross I, Doganci A, Ito Nishimoto N, Yoshizaki K, Kishimoto T, Rose-John S, Galle PR, Neurath MF. 2007. Local blockade of IL-6R signaling induces lung CD4+ T cell apoptosis in a murine model of asthma via regulatory T cells. Int Immunol, 19:685-693.
10
Haghdoost F, Baradaran Mahdavi MM, Zandifari A, Sanei MH, Zolfaghari B, Javanmard SH. 2013. Pistacia atlantica resin has a dose-dependent effect on angiogenesis and skin burn wound healing in the rat. Evid Based Complement Alternat Med, 893425: 1- 8.
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13
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16
Lee SY, Kim JS, Lee JM, Kwon SS, Kim KH, Moon HS, Song JS, Park SH, Kim YK. 2008. Inhaled corticosteroid prevents the thickening of airway smooth muscle in the murine model of chronic asthma. Pulm Pharmacol Ther, 21:9-14.
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18
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19
Minaiyan M, Karimi F, Ghannadi A. 2015. Anti-inflammatory effect of Pistacia atlantica subsp. kurdica volatile oil and gum on acetic acid-induced acute colitis in the rat. Int J pharmacogn, 2: 1-12.
20
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21
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22
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23
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24
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25
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27
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28
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29
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30
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31
ORIGINAL_ARTICLE
Effect of ointment-based egg white on healing of second- degree wound in burn patients: a triple-blind randomized clinical trial study
Objective: Burn wound healing is one of the problems of medical sciences and it is of great importance to find a drug or substance that can heal burn wounds with minimum complications. The present study aimed to evaluate the effect of ointment-based egg white on healing second-degree burn wounds. Materials and Methods: In the present triple-blind clinical trial, a total of 90 patients from Taleghani hospital, Ahvaz, Iran were selected and randomly divided into two groups based on the inclusion criteria. The intervention group was dressed with egg white formulation + silver sulfadiazine cream and the control group was treated with placebo + silver sulfadiazine cream. The burn wound healing process was evaluated on days 1, 7 and 15 by the Bates-Jensen wound assessment tool. Results: The mean scores of wound healing were decreased (13.75±1.83) in the intervention group when compared to the control (21.51±5.7) on day 15 (p<0.001). The mean duration of wound healing, wound depth, edges, undermining, necrotic tissue, amount of necrosis, exudate type and amount, surrounding skin color, wound induration, peripheral edema, granulation, and epithelialization were significantly decrease in intervention group in comparison with control (p<0.001). Conclusion: The findings of this research showed that egg whites formulation is an appropriate treatment for burn wound healing, reduced above-noted burn wounds’ variables. It seems that this treatment, along with the common medicine, improves chronic wound recovery rate and patients’ health status.
https://ajp.mums.ac.ir/article_12597_892da919dcec72ef36767c9c5f87bc0f.pdf
2019-05-01
260
270
10.22038/ajp.2019.12597
Burn
Egg white
Traditional Medicine
silver sulfadiazine
patient
Simin
Jahani
ashrafizadeh.h@ajums.ac.ir
1
Nursing Care Research Center in Chronic Diseases, Nursing and Midwifery School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
AUTHOR
Hadis
Ashrafizadeh
ashrafizadeh.h1993@gmail.com
2
Nursing and Midwifery School, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
LEAD_AUTHOR
Kamran
Babai
babaikamran@yahoo.com
3
Department of Plastic Surgery, Taleghani Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
AUTHOR
Amir
Siahpoosh
amirsiahpoosh@yahoo.com
4
Medical Plants Research Center and Department of Pharmacology, School of Pharmacy, Jundishapur University of Medical Sciences, Ahvaz, Iran.
AUTHOR
Bahman
Cheraghian
cheraghian2000@yahoo.com
5
Department of Biostatistics and Epidemiology, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
AUTHOR
Aalaa M, Malazy OT, Sanjari M, Peimani M, Mohajeri-Tehrani M. 2012. Nurses’ role in diabetic foot prevention and care; a review. J Diabetes Metab Disord, 11: 24-30.
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Alia Sadiq MQH, Gulshan Ara T, Ambreen J. 2018. Effects of essential amino acid “Tryptophan” in post burn skin wound healing. Int J Biosci, 12: 147-153.
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Bahramsoltani R, Farzaei MH, Rahimi R. 2014. Medicinal plants and their natural components as future drugs for the treatment of burn wounds: an integrative review. Arch Dermatol Res, 306: 601-617.
4
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Rastegar F AN, Amiri M, Azarpira A. 2011. The effect of egg yolk oil in the healing of third degree burn wound in rats. Iran Red Crescent Med J, 13: 739-743.
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31
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32
ORIGINAL_ARTICLE
The neuroprotective mechanism of cinnamaldehyde against amyloid-β in neuronal SHSY5Y cell line: The role of N-methyl-D-aspartate, ryanodine, and adenosine receptors and glycogen synthase kinase-3β
Objective: Cinnamaldehyde may be responsible for some health benefits of cinnamon such as its neuroprotective effects. We aimed to investigate the cinnamaldehyde neuroprotective effects against amyloid beta (Aβ) in neuronal SHSY5Y cells and evaluate the contribution of N-methyl-D-aspartate (NMDA), ryanodine, and adenosine receptors and glycogen synthase kinase (GSK)-3β, to its neuroprotective effects. Materials and Methods: After seeding the cells in 96-well plates, adenosine (20, 40, 80, and 120 µM), NMDA (20, 40, 80, and 120 µM), and dantrolene (as a ryanodine receptor antagonist; 2, 4, 6, 8, and 16 µM) were added to the medium containing Aβ25-35 and/or cinnamaldehyde. The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide method was used to assess neurotoxicity and western blot to measure the GSK-3β protein level. Results: Cinnamaldehyde (15, 20, 23, and 25 μM) significantly reversed Aβ-induced toxicity in SHSY5Y neuronal cells. Adenosine (20, 40, 80 and 120 μM) inhibited the neuroprotective effects of cinnamaldehyde (15 μM). NMDA (20, 40, 80, and 120 μM) reduced cinnamaldehyde (15 and 23 μM) neuroprotective effects against Aβ neurotoxicity. Dantrolene (2, 4, 8, and 16 μM) significantly reduced cinnamaldehyde (15 μM) neuroprotective effects. Cinnamaldehyde (15 and 23 μM) suppressed the Aβ-induced increment of GSK-3β protein level. Conclusion: NMDA and adenosine receptors suppression together with ryanodine receptors stimulation may be relevant to cinnamaldehyde neuroprotective effects against Aβ neurotoxicity. Moreover, the inhibition of GSK-3β may contribute to the cinnamaldehyde neuroprotection.
https://ajp.mums.ac.ir/article_12385_0f28a528aa92e306655a178fb4804ea5.pdf
2019-05-01
271
280
10.22038/ajp.2019.12385
Adenosine
Cinnamaldehyde
Dantrolene
Glycogen Synthase Kinase
Neuroprotection
N-methyl-D-aspartate
Masoumeh
Emamghoreishi
emam@sums.ac.ir
1
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
Majid
Farrokhi
farokhim@sums.ac.ir
2
Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
Atena
Amiri
atenaamiri5@gmail.com
3
Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
Mojtaba
Keshavarz
moj.ph60@yahoo.com
4
Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
LEAD_AUTHOR
Allan Butterfield D. 2002. Amyloid β-peptide (1-42)-induced oxidative stress and neurotoxicity: implications for neurodegeneration in Alzheimer's disease brain. A review. Free Radic Res, 36: 1307-1313.
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ORIGINAL_ARTICLE
Effects of Boswellia serrata resin extract on motor dysfunction and brain oxidative stress in an experimental model of Parkinson’s disease
Objective:Boswellia serrata oleo-gum resin (frankincense) exerted antioxidant and anti-inflammatory effects against several diseases, such as; asthma, rheumatoid arthritis and irritable bowel syndrome. In the current study, the influences of B. serrata resin extracton motor dysfunction and oxidative stress markers were investigated in the intrastriatal 6-hydroxydopamine (6-OHDA) model of Parkinson’s disease (PD). Materials and Methods:The animals were randomly assigned to sham, lesion (6-OHDA), and three lesion groups treated with ethyl alcoholic extract of B. serrata at doses of 125, 250 and 500 mg/kg for 3 weeks. The neurotoxin 6-OHDA (12.5 µg) was microinjected into the left striatum to induce PD in male rats. Motor behavior was assessed by rotational and elevated narrow beam tests. Oxidative stress markers were measured in striatal and midbrain homogenates. Results: There was a significant increase in contralateral rotations in 6-OHDA group versus sham group (p<0.001), and treatment with B. serrata resin extract at doses of 125 and 250 mg/kg significantly decreased the rotations in comparison to 6-OHDA group (pB. serrata extract at doses of 125, 250 and 500 mg/kg caused a significant reduction in the latency and total time (p<0.001, p<0.001, and p<0.01, respectively). Biochemical analysis showed no significant difference in oxidative stress markers levels among the groups. Conclusion: Our findings suggest that B. serrata resin extract acts as an anti-inflammatory and antioxidant agent that protects nigrostriatal dopaminergic neurons and improve motor impairments in PD.
https://ajp.mums.ac.ir/article_12403_e3fce5480edc9f7d4c72ff5e1bfde3a9.pdf
2019-05-01
281
290
10.22038/ajp.2019.12403
Boswellia serrata
Motor dysfunction
Oxidative stress
Parkinson’s disease
Parvaneh
Doaee
pdoaee@yahoo.com
1
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
AUTHOR
Ziba
Rajaei
rajaeiz@med.mui.ac.ir
2
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
LEAD_AUTHOR
Mehrdad
Roghani
mehjour@yahoo.com
3
Department of Physiology, School of Medicine, Shahed University of Medical Sciences, Tehran, Iran.
AUTHOR
Hojjatallah
Alaei
alaei@med.mui.ac.ir
4
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I. R. Iran
AUTHOR
Mohammad
Kamalinejad
mkamalinejad@yahoo.com
5
Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
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