%0 Journal Article %T Effects of troxerutin on inflammatory cytokines and BDNF levels in male offspring of high-fat diet fed rats %J Avicenna Journal of Phytomedicine %I Mashhad University of Medical Sciences %Z 2228-7930 %A Hoseindoost, Maryam %A Alipour, Mohammad Reza %A Farajdokht, Fereshteh %A Diba, Roghayeh %A Bayandor, Parvin %A Mehri, Keivan %A Nayebi Rad, Sepehr %A Babri, Shirin %D 2019 %\ 11/01/2019 %V 9 %N 6 %P 597-605 %! Effects of troxerutin on inflammatory cytokines and BDNF levels in male offspring of high-fat diet fed rats %K Troxerutin %K Maternal high fat diet %K BDNF %K Inflammatory cytokines %R 10.22038/ajp.2019.13587 %X Objective: In the present study, we aimed to examine the effect of troxerutin treatment on levels of brain-derived neurotrophic factor (BDNF), and tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), as pro-inflammatory cytokines, in the blood and hippocampus of high-fat diet (HFD) fed dams and their male offspring. Materials and Methods: Forty virgin female Wistar rats, 3 weeks old, were divided into two groups (n=20/per group) and fed control diet (CD), or HFD for 8 weeks. After mating, pregnant animals were assigned to four subgroups including: control (CD), control+troxerutin (CD+T), high-fat diet (HFD), and HFD+troxerutin (HFD+T) groups. HFD was continued during pregnancy and lactation. Troxerutin (150 mg/kg/day, P.O.) was administered during pregnancy in the CD+T and HFD+T groups. On postnatal day (PND) 21, male offspring were separated and fed a normal diet until PND 90. Inflammatory cytokines (TNF-α, and IL-6) and BDNF levels were measured in the serum and hippocampal samples using rat-specific enzyme-linked immunosorbent assay (ELISA) kits. Results: Our findings showed a significant increase in the serum levels of TNF-α and IL-6, but a decrease in BDNF levels in the serum of HFD-fed dams in comparison with CD group, which was reversed by troxerutin. Moreover, troxerutin treatment, during pregnancy, significantly decreased TNF-α and IL-6 levels, but increased BDNF level in the serum and hippocampus of HFD+T offspring in comparison with HFD offspring. Conclusion: These results showed that troxerutin could prevent the harmful effects of maternal HFD on their offspring through inhibition of pro-inflammatory cytokines and elevation of BDNF levels. %U https://ajp.mums.ac.ir/article_13587_9ec1a44ab85bf825feea0bef943ab67c.pdf