eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
366
375
10.22038/ajp.2016.5913
5913
Phytochemical characterization, antimicrobial activity and reducing potential of seed oil, latex, machine oil and presscake of Jatropha curcas
Amit Sharma
mayankgangwar2008@gmail.com
1
Mayank Gangwar
sharmaamitkumar5@gmail.com
2
Dharmendra Kumar
dharmbhu@gmail.com
3
Gopal Nath
gopalnath@gmail.com
4
Akhoury Sudhir Sinha
asksinha.che@itbhu.ac.in
5
Yamini Tripathi
yaminiok@yahoo.com
6
Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi 21005, UP India
Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi- 221005 UP
India
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi- 221005 UP India
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi- 221005 UP India
Department of Chemical Engg, Indian Institute of Technology, Banaras Hindu University, Vanarasi-221005. UP India
Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi 21005, UP India
Objective: This study aims to evaluate the antimicrobial activity, phytochemical studies and thin layer chromatography analysis of machine oil, hexane extract of seed oil and methanol extract of presscake& latex of Jatropha curcas Linn (family Euphorbiaceae). Materials and Methods: J. curcas extracts were subjected to preliminary qualitative phytochemical screening to detect the major phytochemicals followed by its reducing power and content of phenol and flavonoids in different fractions. Thin layer chromatography was also performed using different solvent systems for the analysis of a number of constituents in the plant extracts. Antimicrobial activity was evaluated by the disc diffusion method, while the minimum inhibitory concentration, minimum bactericidal concentration and minimum fungicidal concentration were calculated by micro dilution method. Results: The methanolic fraction of latex and cake exhibited marked antifungal and antibacterial activities against Gram-positive and Gram-negative bacteria. Phytochemical analysis revealed the presence of alkaloids, saponins, tannins, terpenoids, steroids, glycosides, phenols and flavonoids. Reducing power showed dose dependent increase in concentration compared to standard Quercetin. Furthermore, this study recommended the isolation and separation of bioactive compounds responsible for the antibacterial activity which would be done by using different chromatographic methods such as high-performance liquid chromatography (HPLC), GC-MS etc. Conclusion: The results of the above study suggest that all parts of the plants possess potent antibacterial activity. Hence, it is important to isolate the active principles for further testing of antimicrobial and other biological efficacy.
https://ajp.mums.ac.ir/article_5913_62ae25cce35dd4a0bc99253017ad800d.pdf
Reducing power
Antimicrobial
Phytochemical
Thin layer chromatography
Minimum bactericidal concentration
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
376
382
10.22038/ajp.2016.5961
5961
In vitro protoscolicidal effects of various concentrations of Ziziphora tenuior L. extract at different exposure times
Mojtaba Shahnazi
shahnazi58@yahoo.com
1
Abbas Azadmehr
azadmehr2014@gmail.com
2
Robabeh Latiffi
azadmehr2012@gmail.com
3
Reza Hajiaghaee
rhajiaghaee@yahoo.com
4
Mehrzad Saraei
azadmehr2010@gmail.com
5
Mahmood Alipour
shahnazi2013@gmail.com
6
Department of Parasitology, Qazvin University of Medical Sciences, Qazvin, Iran
Department of Immunology, Babol University of Medical Sciences, Babol, Iran
Department of Parasitology, Qazvin University of Medical Sciences, Qazvin, Iran
Department of Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Department of Parasitology, Qazvin University of Medical Sciences, Qazvin, Iran
Department of Social Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
Objective: Hydatidosis is one of the most important zoonotic diseases and surgery is still the main treatment for this problem. One of the side effects of hydatid cyst surgery is recurrence, thus, searching and assessment of some new agents such as medicinal plant extracts are very important. In the present study, the scolicidal effect of ethanolic extract of Ziziphora tenuior L. (Z. tenuior) was investigated. Materials and Methods: Protoscolices were aseptically collected from sheep livers containing hydatid cyst and used in the experiments. Z. tenuior extract was used at concentration of 3-100 mg/ml for 10-60 min. Viability of protoscolices was determined by 0.1% eosin staining. Results: Based on our results, Z. tenuior extract at concentration of 10 mg/ml killed all protoscolices after 20 min. However, this medicinal plant at concentration of 25 mg/ml destroyed all protoscolices in a shorter exposure time (10 min). Therefore, the scolicidal activity of the extract at 10 and 25 mg/ml concentrations was considerably effective in lower concentrations and shorter exposure times. Conclusion: The findings of this study showed that the ethanolic extract of Z. tenuior produces high scolicidal activity; it may be used as an appropriate and effective scolicidal agent in hydatidosis surgery. This is the first report on the protoscolicidal activity of Z. tenuior.
https://ajp.mums.ac.ir/article_5961_9ab9f6472467576921199ddb5909e851.pdf
Hydatidosis
Surgery
Medicinal plant
Scolicidal
Hydatid Cyst
Ziziphoratenuior L
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
383
398
10.22038/ajp.2016.6187
6187
Novel delivery system for natural products: Nano-curcumin formulations
Hamid Reza Rahimi
rahimihr@mums.ac.ir
1
Reza Nedaeinia
nedaeinr901@mums.ac.ir
2
Alireza Sepehri Shamloo
sepehria871@gmail.com
3
Shima Nikdoust
nikdousts901@mums.ac.ir
4
Reza Kazemi Oskuee
oskueekr@mums.ac.ir
5
Students Research Committee, Mashhad University of Medical Sciences, Iran
Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Students Research Committee, Mashhad University of Medical Sciences, Iran
Students Research Committee, Mashhad University of Medical Sciences, Iran
Neurogenic Inflammation Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Objective: Curcumin is extracted from Curcuma longa and regulates the intracellular signal pathways which control the growth of cancerous cell, inflammation, invasion and apoptosis. Curcumin molecules have special intrinsic features that can target the intracellular enzymes, genome (DNA) and messengers (RNA). A wide range of studies have been conducted on the physicochemical traits and pharmacological effects of curcumin on different diseases like cardiovascular diseases, diabetes, cancer, rheumatoid arthritis, Alzheimer’s, inflammatory bowel disease (IBD), and even it has wound healing. Oral bioavailability of curcumin is rather poor, which would certainly put some boundaries in the employment of this drug. Materials and Methods: Bibliographical searches were performed using MEDLINE/ScienceDirect/OVID up to February 2015 using the following keywords (all fields): (“Curcumin” OR “Curcuma longa”) AND [(nanoparticles) OR (Nanomicelles) OR (micro emulsions) OR (liposome) OR (phospholipid). Results: Consequently, for any developments of curcumin in the future, analogues of curcumin that have better bioavailability or substitute formulations are needed crucially. Conclusion: These studies indicated that nanotechnology can formulate curcumin effectively, and this nano-formulated curcumin with a potent ability against various cancer cells, were represented to have better efficacy and bioavailability under in vivo conditions.
https://ajp.mums.ac.ir/article_6187_fa00aef51aa9891d1f1325eaef72a8a9.pdf
Curcumin
Curcumin Nano formulations
Nano micelle
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
399
409
10.22038/ajp.2016.6261
6261
Neuroprotective effects of Withania coagulans root extract on CA1 hippocampus following cerebral ischemia in rats
Maryam Sarbishegi
sarbishegi@yahoo.co.in
1
Zahra Heidari
histology_iri@yahoo.com
2
Hamidreza Mahmoudzadeh- Sagheb
histology@ymail.com
3
Mohharam Valizadeh
m.valizadeh@anrs.usb.ac.ir
4
Mahboobeh Doostkami
mahtab amiri@yahoo.com
5
Department of Anatomy, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Infectious Diseases and Tropical Medicine Research Center, and Department of histology, School of Medicine, Zahedan University of Medical sciences, Zahedan, Iran
Department of Histology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Department of Medicinal and Aromatic Plant, High Complex Education of Saravan, Saravan, Iran
Department of Anatomy, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Objective: Oxygen free radicals may be implicated in the pathogenesis of ischemia reperfusion damage. The beneficial effects of antioxidant nutrients, as well as complex plant extracts, on cerebral ischemia-reperfusion injuries are well known. This study was conducted to determine the effects of the hydro-alcoholic root extract of Withania coagulans on CA1 hippocampus oxidative damages following global cerebral ischemia/reperfusion in rat. Materials and Methods: Male Wistar rats were randomly divided in five groups: control, sham operated, Ischemia/ Reperfiusion (IR), and Withania Coagulans Extract (WCE) 500 and 1000mg/kg + I/R groups. Ischemia was induced by ligation of bilateral common carotid arteries for 30 min after 30 days of WCE administration. Three days after, the animals were sacrificed, their brains were fixed for histological analysis (NISSL and TUNEL staining) and some samples were prepared for measurement of malondialdehyde (MDA) level and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activity in hippocampus. Results: WCE showed neuroprotective activity by significant decrease in MDA level and increase in the SOD, CAT and GPx activity in pretreated groups as compared to I/R groups (p<0.001). The number of intact neurons was increased while the number of TUNEL positive neurons in CA1 hippocampal region in pretreated groups were decreased as compared to I/R group (p<0.001). Conclusion: WCE showed potent neuroprotective activity against oxidative stress-induced injuries caused by global cerebral ischemia/ reperfusion in rats probably by radical scavenging and antioxidant activities.
https://ajp.mums.ac.ir/article_6261_0eae4589c4520c6e283ea944c482523a.pdf
Withania coagulans
Antioxidant Enzymes
Hippocampus
Ischemia
Rat
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
410
417
10.22038/ajp.2016.6263
6263
Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines
Mohamad Ali Geryani
geryanima@mums.ac.ir
1
Davood Mahdian
mahdiand881@mums.ac.ir
2
Seyed Hadi Mousavi
mousavih@mums.ac.ir
3
Azar Hosseini
hoseiniaz@mums.ac.ir
4
Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Objective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-proliferative and pro-apoptotic effects of flower extract of P. abrotanoides on MCF-7 and Hela cell lines. Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin and 100 µg/ml streptomycin and incubated with different concentrations of plant extracts. Cell viability was quantified by MTT assay. Apoptotic cells were determined using propidium iodide (PI) staining of DNA fragmentation by flow cytometry (sub-G1 peak). Results: P. abrotanoides extract inhibited the growth of malignant cells in a time and dose-dependent manner and 1000 µg/ml of extract following 48h of incubation was the most cytotoxic dose against Hela cell in comparison with other doses; however, in MCF-7 cells,1000 and 500 µg/ml PA induced toxicity at all time points but with different features.. Analysis of flowcytometry histogram of treated cells compared with control cells indicated that the cytotoxic effect is partly due toapoptosis induction. Conclusion: Hydro-alcoholic extract of P. abrotanoides flowers inhibits the growth of MCF-7 and HeLa cell lines, partly via inducing apoptosis. Their inhibitory effect was increased in a time and dose-dependent manner, especially in MCF7 cells. However, further studies are needed to reveal the mechanisms of P. abrotanoides extract-induced cell death.
https://ajp.mums.ac.ir/article_6263_230bf2d1ce8678f7e5684bf83ca7ee5f.pdf
Perovskia abrotanoides
Breast Cancer
Cervical Cancer
Apoptosis
MCF-7
HeLa
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
418
424
10.22038/ajp.2016.6351
6351
Effects of Persian leek (Allium ampeloprasum) on hepatic lipids and the expression of proinflammatory gene in hamsters fed a high-fat/ high-cholesterol diet
Vahideh Fatoorechi
vahideh.fatoorechi@gmail.com
1
Marjan Rismanchi
rismanchimarjan@yahoo.com
2
Javad Nasrollahzadeh
jnasrollahzadeh@gmail.com
3
Department of Clinical Nutrition and Dietetics, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Faculty of Nutrition Sciences and Food Technology, ShahidBeheshti University of Medical Sciences, Tehran, Iran
National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Department of Clinical Nutrition & Dietetics, ShahidBeheshti University of Medical Sciences, Tehran, Iran
Objective: Persian leek is one of the most widely used herbal foods among Iranians. In this study, effects of oral administration of Persian leek on plasma and liver lipids were examined in hamster. Materials and Methods: Male Syrian hamsters were randomly divided into three groups: control (standard diet), high fat control (high-fat/high-cholesterol diet), Persian leek (high-fat/high-cholesterol diet + 1% per weight of diet from dried powdered Persian leek) for 14 weeks. Results: High fat diet increased plasma and liver lipids as compared to standard diet. Adding Persian leek to the high-fat/high-cholesterol diet resulted in no significant changes in the concentration of the plasma lipids or liver cholesterol. However, liver triglycerides (TG), plasma Alanine aminotransferase and gene expression of tumor necrosis factor- α were decreased in hamsters fed high-fat diet containing Persian leek as compared to high-fat diet only. Conclusion: Persian leek might be considered as a herbal food that can reduce liver TG accumulation induced by high fat diets.
https://ajp.mums.ac.ir/article_6351_7748482ae108974fe447938a96916a0c.pdf
Leek
High fat
Liver lipids
Hyperlipidemia
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
425
433
10.22038/ajp.2016.6377
6377
Phytochemical screening and evaluation of antioxidant activities of Dracocephalum kotschyi and determination of its luteolin content
Mansureh Kamali
kamali_1352@yahoo.com
1
Susan Khosroyar
susankhosroyar@yahoo.com
2
Hossein Kamali
h.kamali62@yahoo.com
3
Tooba Ahmadzadeh Sani
npsmp.rc@gmail.com
4
Ameneh Mohammadi
ameneh.mohamadi@yahoo.com
5
Department of Chemical Engineering, Quchan Azad University, Quchan, Iran
Department of Chemical Engineering, Quchan Azad University, Quchan, Iran
Natural Products & Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Natural Products & Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
Objective: Dracocephalum kotschyi (Lamiaceae family) has been usedin traditional medicine for stomach and liver disorders, headache and congestion. In the present study, we have investigated phytochemical properties and antioxidant activities of dichloromethane, ethyl acetate and methanol extracts of D.kotschyi. Material and Methods: Antioxidant activities of extracts were evaluated using the integration of HPLC-DPPH and ferric reducing antioxidant power (FRAP) methods. In addition, the luteolincontent was determined using HPLC method. Results: The highest antioxidant activity was observed for the methanol extract (among the three tested extracts) showing 50% DPPH scavenging activity at 4.85µg/ml as compared to butylated hydroxy toluene (BHT) and ascorbic acid (3.00 µg/ml, 0.97 µg/ml). Also, luteolin was detected in methanol extract; it was identified by comparing its retention time and DAD spectra with standard and it was one of antioxidant components of this plant. In addition, the antioxidant activity of methanol extract was higher than BHT, in FRAP assay. Total phenolic content was in the range of 11.62-22.29 mg Gallic acid /gram of dry extract and flavonoid content was in the range of 3.97-5.042 mg Quercetin/ gram of extract for dichloromethane, ethyl acetate and methanol extracts. The quantity of luteolin in D.kotschyiwas found to be 1061.005 µg/g of dried plant. Conclusion: The results of this investigation indicated that luteolin plays major role in the antioxidant activity of the plant.
https://ajp.mums.ac.ir/article_6377_c1cecac2bf89b960665aa0e4382355aa.pdf
Antioxidant
Dracocephalum kotschyi
Luteolin
HPLC
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
434
441
10.22038/ajp.2016.6327
6327
Protective effect of Viola tricolor and Viola odorata extracts on serum/glucose deprivation-induced neurotoxicity: role of reactive oxygen species
Seyed Hadi Mousavi
mousavih@mums.ac.ir
1
Behnaz Naghizade
behnaz5287@yahoo.com
2
Solmaz Pourgonabadi
pourgonabadip891@mums.ac.ir
3
Ahmad Ghorbani
ghorbania@mums.ac.ir
4
Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Payam Noor Shargh, Tehran University of Basic Sciences, Tehran, Iran
Department of Pharmacology School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Objective: Oxidative stress plays a key role in the pathophysiology of brain ischemia and neurodegenerative disorders.Previous studies indicated that Viola tricolor and Viola odorataare rich sources of antioxidants. This study aimed to determine whether these plants protect neurons against serum/glucose deprivation (SGD)-induced cell death in an in vitro model of ischemia and neurodegeneration. Methods and Material: The PC12 neuronal cells were pretreated for 4 hr with 1 to 50 µg/ml of V. odorata or V. tricolor hydroalcoholic extracts followed by 24 hr incubation under SGD condition. Cell viability was evaluated by 4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2H-tetrazolium bromide assay. The level of intracellular reactive oxygen species (ROS) was quantitated by flow cytometry using 2',7'- dichlorofluorescin diacetate as a probe. Results: SGD condition led to significant decrease in cell viability (p < 0.001). Pretreatment with both V. tricolor and V. odorata extracts reduced the SGD-induced cytotoxicity. SGD resulted in a significant increase in intracellular ROS production (p < 0.001). Both extracts at concentrations of 25 and 50 µg/ml could reverse the increased ROS production (p < 0.05). Conclusion: Results of the present study showed that V. tricolor and V. odorata protect neuronal cells against SGD-induced cell death, at least in part, by their antioxidant activities. Further studies on the possible application of these plants in prevention or treatment of cerebral ischemia and neurodegenerative diseases seem to be warranted.
https://ajp.mums.ac.ir/article_6327_a56afacd18d062f7e9e5eab94bfd931c.pdf
PC12
Reactive Oxygen Species
Viola tricolor
Viola odorata
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
442
448
10.22038/ajp.2016.6400
6400
The effects of crocin and safranal on the yawning induced by intracerebroventricular injection of histamine in rats
Mina Taati
minataati68@gmail.com
1
Amir Erfanparast
erfanparast@gmail.com
2
Esmaeal Tamaddonfard
etamaddonfard@yahoo.com
3
Hamid Ghasemi
ghasemi.h1986@gmail.com
4
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 57153-1177, Iran
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 57153-1177, Iran
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 57153-1177, Iran
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 57153-1177, Iran
Objective: Crocin and safranal, as the major constituents of saffron, have many biological activities. This study investigated the effects of crocin and safranal on yawning response induced by intracerebroventricular (i.c.v.) injection of histamine in rats. Materials and Methods: In ketamine/xylazine-anesthetized rats, a guide cannula was implanted in the right ventricle of the brain and yawning induced by i.c.v. injection of histamine. Crocin and safranal were intraperitoneally (i.p.) injected alone and before i.c.v. injection of histamine. Results: Histamine at the doses of 10 and 20 µg/rat produced yawning. Mepyramine (a histamine H1 receptor antagonist) 40 µg/rat significantly (p<0.05) prevented histamine (20 µg/rat)-induced yawning. Crocin (30 mg/kg) and safranal (1 mg/kg) significantly (p<0.05) increased histamine (10 µg/rat)-induced yawning. Crocin and safranal also induced yawning when injected before mepyramine plus histamine administration. Conclusion: The results of the present study showed a yawning-inducing effect for central histamine, which was inhibited by mepyramine. Crocin and safranal increased histamine-induced yawning, and also produced yawning when the histamine action is blocked.
https://ajp.mums.ac.ir/article_6400_1795218df9706173f7d288252309be2c.pdf
Crocin
Histamine
Safranal
Yawning
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
449
457
10.22038/ajp.2016.6451
6451
Hydroalcoholic extract of needles of Pinus eldarica enhances pentobarbital-induced sleep: possible involvement of GABAergic system
Fatemeh Forouzanfar
forouzanfarf901@mums.ac.ir
1
Ahmad Ghorbani
ghorbania@mums.ac.ir
2
Hasan Rakhshandeh
rakhshandehh@mums.ac.ir
3
Mahmoud Hosseini
sfsfsdfs@mums.ac.ir
4
Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Neurocognitive Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Objective: Insomnia is accompanied by several health complications and the currently used soporific drugs can induce several side effects such as psychomotor impairment, amnesia, and tolerance. The present study was planned to investigate the sleep prolonging effect of Pinus eldarica. Materials and Methods: Hydroalcoholic extract (HAE) of P. eldarica, its water fraction (WF), ethyl acetate fraction (EAF) and n-butanol fraction (NBF) were injected (intraperitoneally) to mice 30 min before administration of pentobarbital. Then, the latent period and continuous sleeping time were recorded. Also, LD50 of P. eldarica extract was determined and the possible neurotoxicity of the extract was tested on neural PC12 cells. Results: The HAE and NBF decreased the latency of sleep (p<0.05) and significantly increased duration of sleep (p<0.05) induced by pentobarbital. These effects of P. eldarica were reversed by flumazenil. The LD50 value for HAE was found to be 4.8 g/Kg. HAE and its fractions did not show neurotoxic effects in cultured PC12-cell line. Conclusion: The present data indicate that P. eldarica potentiated pentobarbital hypnosis without major toxic effect. Most probably, the main components responsible for this effect are non-polar agents which are found in NBF of this plant.
https://ajp.mums.ac.ir/article_6451_901d9c6fef738f7f7d78a95c05e75cff.pdf
insomnia
Diazepam
Pinuseldarica
Sleep
PC12
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
458
467
10.22038/ajp.2016.6455
6455
The effect of Allium sativum (Garlic) extract on infectious bronchitis virus in specific pathogen free embryonic egg
Tabassom Mohajer Shojaei
mohajer.tabassom1991@gmail.com
1
Arash Ghalyanchilangeroudi
arashghalyanchi@gmail.com
2
Vahid Karimi
vkarimi@ut.ac.ir
3
Abbas Barin
abarin05@gmail.com
4
Naser Sadri
naser.sadri@ymail.com
5
Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Objective: Garlic is a plant has been used as a flavor, and anti-microbial and anti-diarrheal agent. Infectious bronchitis virus (IBV) is a coronavirus. The available vaccines against IBV cannot cover new variants. This study evaluated the inhibitory effects of garlic extract on IBV. Materials and Methods: The constituents of garlic extract were detected by gas chromatography. This study was done in four groups of embryonic SPF eggs; first group was used for virus titration; second group received the mixture of different virus titration and constant amount of garlic extract; third group received 10-3 titration of virus and after 8 hr received garlic extract and the last group received different dilutions of garlic extract. Results: Based on our results, in the second group, IBV vaccine strain (4/91) at all titration and M41 in 10-2 and 10-3 titration and in the third group both variants of virus the embryonic Index (EI) was significantly increased. Conclusion: The garlic extract had inhibitory effects on IBV in the chickens embryo.
https://ajp.mums.ac.ir/article_6455_b12ce3bc382a2e65f28416324779d1d6.pdf
Infectious Bronchitis Virus
Garlic extract
Embryonic Index
Herbal Medicine
Antiviral
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
468
475
10.22038/ajp.2016.6465
6465
Therapeutic effect of frankincense in a rat model of Alzheimer’s disease
Siamak Beheshti
siamak.beheshti@yahoo.com
1
Rezvan Aghaie
rezvans68@yahoo.com
2
Division of Animal Science, Department of Biology, Faculty of Science, University of Isfahan
Division of Animal Sciences, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
Objective: Frankincense improves memory in different models of learning. However, its influence on models of Alzheimer's disease (AD) has not been studied widely. In the present study, the therapeutic effect of frankincense was evaluated in a model of AD induced by i.c.v administration of streptozotocin. Materials and Methods: Under stereotaxic surgery, two guide cannulas were implanted in the lateral ventricles of adult male Wistar rats weighing 230-270 g. One group received streptozotocin (1.5 mg/kg/2μl/side) bilaterally on the first and third day of surgery. Another group received artificial cerebro-spinal fluid. Fourteen days after surgery, learning was evaluated using the passive avoidance paradigm. Four other groups of animals received frankincense (50 mg/kg) or its solvent after establishment of AD for 21 or 42 consecutive days, and then, memory retrieval was assessed. Results: Streptozotocin increased the number of stimulations required for induction of short-term memory and decreased step-through latency on the test day, significantly (p<0.05). Chronic injection of the aqueous extract of frankincense for 21 days did not affect learning parameters, but injection of it for 42 days, significantly increased step-through latency (p<0.05), decreased the number of step-through into the dark compartment (p<0.01) and decreased the time spent in the dark compartment (p<0.05). Conclusion: The results indicate that chronic administration of frankincense has the potential to improve dementia type of AD induced by i.c.v injection of streptozotocin in a time-dependent manner.
https://ajp.mums.ac.ir/article_6465_6c970c3e7339e3c5fd66254fc7b7cba1.pdf
Streptozotocin
Alzheimer’s disease
Frankincense
Memory
Rat
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2016-07-01
6
4
476
488
10.22038/ajp.2016.6701
6701
Anxiolytic-like effect of ethanolic extract of Argemone mexicana and its alkaloids in Wistar rats
Aideé Itzel Arcos-Martínez
aidearcos@hotmail.com
1
Omar David Muñoz-Muñiz
omunoz@uv.mx
2
Miguel Ángel Domínguez-Ortiz
midominguez@uv.mx
3
Margarita Virginia Saavedra-Vélez
mago_68@hotmail.com
4
Maribel Maribel Vázquez-Hernández
marivazquez@uv.mx
5
Maria Gabriela Alcantara-Lopez
gaviota2571@gmail.com
6
Institute of Neuroethology, University of Veracruz (UV), Xalapa-Veracruz, Mexico
Support Services Unit in Analytical Resolution (SARA), University of Veracruz (UV), Xalapa-Veracruz, Mexico
Institute of Basic Sciences (ICB), University of Veracruz (UV), Xalapa-Veracruz, Mexico
Institute of Neuroethology, University of Veracruz (UV), Xalapa-Veracruz, Mexico
Support Services Unit in Analytical Resolution (SARA), University of Veracruz (UV), Xalapa-Veracruz, Mexico
Faculty of Pharmaceutical and Biological Chemistry (QFB), University of Veracruz (UV), Xalapa-Veracruz, Mexico
Objective: Argemone mexicana is a Papaveracea plant; some reports have shown their antibacterial, anti-cancer, sedative and probably anti-anxiety properties. From their aerial parts, flavonoids and alkaloids have been isolated, which are intrinsically related to some actions on the central nervous system. The aim of this study was to evaluate the anxiolytic-like effects of the plant, using its ethanolic extract and alkaloid-enriched extract obtained from fresh leaves. Material and Methods: Phytochemical screening was carried out together with evaluation of antioxidant capacity and the enrichment of alkaloids present in the extract. Subsequently, 100 and 200 mg/kg doses of ethanolic extract and alkaloid-enriched extract (200 µg/kg) were intraperitoneally administered to female Wistar rats, which were exposed to elevated plus maze (EPM) test. Picrotoxin (1 mg/kg), a non-competitive gamma-aminobutyric acid A (GABAA) chloride channel antagonist, was used in experimental procedures to evaluate if this receptor is involved in the anxiolytic-like effects of A. mexicana. To discard motor effects associated with the treatments, the rats were evaluated by the locomotor activity test. Results: Only the ethanolic extract at 200 mg/kg and alkaloid-enriched extract (200 µg/kg) produced anxiolytic-like effects similarly to diazepam 2 mg/kg on EPM test, without affecting locomotor activity. Meanwhile, the administration of picrotoxin blocked anti-anxiety effect of alkaloid-enriched extract of the plant. Conclusion: These results showed that A. mexicana is a potential anxiolytic agent and we suggest that this effect is mediated by the GABAA receptor. These effects are related to the presence of alkaloids.
https://ajp.mums.ac.ir/article_6701_d5ecdcfdabed6e1f87ea70f49bf6a7ca.pdf
Argemone mexicana
Alkaloids
GABAA receptor antagonist
Anxiolytic-like effect