eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2017-05-01
7
3
199
205
10.22038/ajp.2016.6600
6600
Assessment of immunomodulatory activity of aqueous extract of Calamus rotang
Amit Gupta
amitvsbt@gmail.com
1
Sushama Ravindra Chaphalkar
amitgupta@vsbt.res.in
2
Vidya Pratishthan’s School of Biotechnology (VSBT, Research centre affiliated to Savitribai Phule Pune University) Baramati, Maharashtra, India
Vidya Pratishthan’s School of Biotechnology (VSBT, Research centre affiliated to Savitribai Phule Pune University) Baramati, Maharashtra, India
Objective: There are a number of medicinal plant products which has been used to treat various immunological diseases. Out of these medicinal plants, Calamus rotang has shown several medicinal properties including anti-viral, anti-diabetic, anti-inflammatory effects. Normally, the roots of C. rotang are used in various ailments to cure piles, burning sensation, cough, leprosy and bleeding disorder and also it was used in the treatment of inflammation. In the present study, our group were investigated the immunomodulatory activity of aqueous extract of C. rotang root in human whole blood and peripheral blood mononuclear cells (PBMC) using hepatitis B vaccine (HBsAg) as an antigen. Materials and Methods: Variable doses of root aqueous extract (0.5 – 30 mg/ml, 100 µl) was administered to human whole blood and PBMC using hepatitis B vaccine containing surface antigen (HBsAg; 20 µg/ml, 10 µl) as specific antigen in order to estimate the total blood counts in human whole blood and nitric oxide production and CD14 FITC surface marker from human PBMC. Results: Overall, the results showed that roots aqueous extract of C. rotang showed remarkable increase in the number of blood counts in human whole blood at lower doses (0.5 mg/ml). In addition, root aqueous extract of C. rotang also showed the same pattern in case of nitric oxide production and estimation of CD14 FITC surface marker in human PBMC. Conclusion: Altogether, the results suggest that root aqueous extract of C. rotang showed immunomodulatory activity.
https://ajp.mums.ac.ir/article_6600_c98cdbcbc4138ecaf8166b09bff3f445.pdf
Calamus rotang
Immunomodulatory
Aqueous extract
PBMC
Hepatitis B vaccine
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2017-05-01
7
3
206
213
10.22038/ajp.2016.7760
7760
Therapeutic efficacy of rose oil: A comprehensive review of clinical evidence
Safieh Mohebitabar
s-mohebitabar@razi.tums.ac.ir
1
Mahbobeh Shirazi
mahboobeh.shirazi@yahoo.in
2
Sodabeh Bioos
so.bioos@gmail.com
3
Roja Rahimi
rahimi_r@razi.tums.ac.ir
4
Farhad Malekshahi
malekshahi56@gmail.com
5
Fatemeh Nejatbakhsh
fnejatbakhsh@yahoo.com
6
Department of Iranian Traditional Medicine, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran
Maternal, Fetal, and Neonatal Research Center, Tehran University of Medical Sciences, Tehran, Iran
Department of Iranian Traditional Medicine, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran
Department of Traditional Pharmacy, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran
Department of management, School of management and accounting, Emam Hosein University, Tehran, Iran
Department of Iranian Traditional Medicine, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran
Objective: Rose oil is obtained from the petals of difference Rosa species especially Rosa centifolia L. and Rosa damascena Mill. Various pharmacological properties have been attributed to rose oil. The aim of the present study was to review the rose oil therapeutic effects which had been clinically evaluated in trial studies. Materials and Methods: Google scholar, PubMed, Cochrane Library, and Scopus were searched for human studies which have evaluated the therapeutic effects of rose oil and published in English language until August 2015. Results: Thirteen clinical trials (772 participants) were included in this review. Rose oil was administered via inhalation or used topically. Most of the studies (five trials) evaluated the analgesic effect of rose oil. Five studies evaluated the physiological relaxation effect of rose oil. Anti-depressant, psychological relaxation, improving sexual dysfunction, and anti-anxiety effects were the other clinical properties reported for rose oil. Conclusion: Numerous studies on the pharmacological properties of rose oil have been done in animals, but studies in humans are few. In this study, it was observed that rose oil had physiological and psychological relaxation, analgesic and anti-anxiety effects. To obtain conclusive results on the efficacy and safety of rose oil, further clinical trials with larger sample size and better designation are required.
https://ajp.mums.ac.ir/article_7760_7a0383b781e34e4c99038b38f34faf93.pdf
Rosa damascena
Rose oil
comprehensive review
human studies
Clinical trial
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2017-05-01
7
3
214
222
10.22038/ajp.2016.7793
7793
Potentiating effects of Perovskia abrotanoides on pentobarbital-induced sleep
Fatemeh Forouzanfar
forouzanfarf901@mums.ac.ir
1
Azar Hosseini
hoseiniaz@mums.ac.ir
2
Mohammad Sadegh Amiri
amiriherb@gmail.com
3
Hasan Rakhshandeh
rakhshandehh@mums.ac.ir
4
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Biology, Payame Noor University, Tehran, Iran
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
Objective: Sleeplessness is the most common sleep disorder. In this study the hypnotic effect of the hydro-alcoholic extract (HAE) of Perovskia abrotanoides and its water fraction (WF), ethyl acetate fraction (EAF) and n-butanol fraction (NBF) were studied in mice.Materials and Methods: The test compounds were administered intraperitoneally to mice 30 min before the administration of sodium pentobarbital (30 mg/kg.). Moreover, the influence of flumazenil on the hypnotic effect of the extracts was evaluated. Besides, 30 min after administration of HAE, motor coordination (rota-rod test) was assessed. Additionally, LD50 for HAE was determined and the possible neurotoxicity of the extract was tested in neural PC12 cells.Results: The HAE and NBF decreased the latency of sleep (p<0.05), and significantly increased the duration of sleep (p<0.05) induced by pentobarbital. These effects of P. abrotanoides were reversed by flumazenil. HAE did not affect the animals’ performance on the rota-rod test. The LD50 value for HAE was found to be 4.8 g/kg. HAE and its fractions did not show neurotoxic effects in cultured PC12 cell line.Conclusion: The results showed that P. abrotanoides significantly potentiated pentobarbital hypnosis without toxic effect. Probably, its effects are related to its non-polar constituents.
https://ajp.mums.ac.ir/article_7793_4dea0a5774b93610d099508ddb173a4a.pdf
Perovskia abrotanoides
Diazepam
Sleep
PC12
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2017-05-01
7
3
223
231
10.22038/ajp.2016.7882
7882
Evaluation of fibrinolytic and antioxidant effects of Allium elburzense bulb extracts
Leila Safaeian
leila_safaeian@pharm.mui.ac.ir
1
Behzad Zolfaghari
behzadz@gmail.com
2
Mohammadreza Aghaye-Ghazvini
mr-ghazvini@farabi.tums.ac.ir
3
Mahya Behnampour
mahya.behnampour@gmail.com
4
Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Isfahan Center of Public Health Training and Research, Institute of Public Health Research, Tehran University of Medical Science, Iran
Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Objective: Allium elburzense is an endemic plant of the family Amaryllidaceae that grows wild in northern Iran with some nutritional and medicinal applications. The present study was aimed to investigate the fibrinolytic and antioxidant effects of A. elburzense bulb extracts. Materials and Methods: Hydroalcoholic, aqueous, chloroformic and butanolic extracts were evaluated in this research. In vitro antioxidant assays were performed using total phenolic, DPPH, and FRAP methods. In the in vivo analysis, animals receivedi.p. injection of A. elburzense hydroalcoholic extract for 21 days and hydroperoxides level, FRAP value, PT and aPTT were determined in serum samples. The fibrinolytic activity of different extracts was quantitatively evaluated by measurement of clot weight. Results: In vitro antioxidant assay showed that A. elburzense aqueous extract had the highest DPPH scavenging and the highest total antioxidant capacity. In the in vivo assay, A. elburzense hydroalcoholic extract reduced serum hydroperoxides level and increased serum total antioxidant capacity in rats.In vitro fibrinolytic assay revealed remarkable thrombolytic activity for this plant with the highest effect for the aqueous extract. However, coagulation parameters including PT and aPTT were not affected by administration of A. elburzense hydroalcoholic extract in rats. Conclusion: In conclusion, the results of this study revealed the potential antioxidant and fibrinolytic effects of A. elburzense bulbextracts. For developing novel thrombolytic agents, further investigations for isolation of bioactive constituents and finding the underlying mechanisms are suggested.
https://ajp.mums.ac.ir/article_7882_2f3addb334315a6f8c04431c9a1df234.pdf
Allium elburzense Wendelbo
Antioxidant
Fibrinolytic agents
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2017-05-01
7
3
232
241
10.22038/ajp.2017.18637.1721
8169
The effects of setarud on the immunological status of HIV-positive patients: Efficacy of a novel multi-herbal drug
Mehdi Gholamzadeh Baeis
dr.m.gholamzadeh@gmail.com
1
Ghasem Amiri
dr.ghasem.amiri@gmail.com
2
Mojtaba Miladinia
miladimojtaba@gmail.com
3
Young Researchers and Elites Club, Qom Branch, Islamic Azad University, Qom, I. R. Iran
Infectious disease specialist, Qom University of Medical Sciences, Qom, I. R. Iran.
Nursing Care Research Center in Chronic Diseases, School of Nursing and Midwifery, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, I. R. Iran
Objective: This study examines the effect of the addition of IMOD, a novel multi-herbal drug to the highly active anti-retroviral therapy (HAART) regimen, on the immunological status of HIV-positive patients. Materials and Methods: A randomized two-parallel-group (HAART group versus HAART+IMOD group), pretest-posttest design was used.Sixty patients with indications for treatment with the HAART regimen participated. One week before and 2 days after the treatments, immunological parameters including total lymphocyte count (TLC) and CD4 cell count were assessed.The intervention group received the HAART regimen plus IMOD every day for 3 months. The control group received only the HAART regimen every day for 3 months. Results: In the intervention group, a significant difference was observed in CD4between before and after drug therapy (CD4 was increased). However, in the control group, the difference in CD4 was not significant before and after drug therapy. The difference in TLC was not significantly different between the two groups before and after therapy. Nevertheless, TLC was higher in the intervention group. Conclusion: IMOD (as a herbal drug) has been successfully added to the HAART regimen to improve the immunological status of HIV-positive patients.
https://ajp.mums.ac.ir/article_8169_54caab9355d49bc7a705197acc62b1e5.pdf
Herbal Therapy
Anti-AIDS drugs
IMOD
HAART
HIV
CD4 Cell Counts
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2017-05-01
7
3
242
249
10.22038/ajp.2016.15567.1615
8170
Effect of linalool on the acquisition and reinstatement of morphine-induced conditioned place preference in mice
Narjes Pourtaqi
1
Mohsen Imenshahidi
ImenM@mums.ac.ir
2
Bibi Marjan Razavi
razavimr@mums.ac.ir
3
Hossein Hosseinzadeh
hosseinzadehh@mums.ac.ir
4
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Pharmaceutical Research Center, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Targeted Drug Delivery Research Center, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Pharmaceutical Research Center, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Objective: The effect of linalool, a terpene alcohol found in many plants, which inhibits NMDA receptors, on the acquisition and reinstatement of morphine-induced conditioned place preference (CPP) was evaluated in mice.Material and Methods: The effects of different doses of linalool (12.5, 25 and 50 mg/kg, i.p.), memantine (20 mg/kg, an NMDA receptor antagonist) and saline, in CPP induced by 40 mg/kg of morphine were investigated in mice. In another experiment, a single injection of morphine (10 mg/kg) reinstated the place reference following extinction of a place preference induced by morphine (40 mg/kg). Linalool (12.5, 25 and 50 mg/kg, i.p.), memantine (20 mg/kg) and saline were administrated 30 min before this priming dose of morphine.Results: In the first experiment, linalool (12.5 and 50 mg/kg) was able to decrease morphine-induced CPP. In the second part, linalool (25 and 50 mg/kg) reduced morphine-induced reinstatement of place preference. Both acquisition and reinstatement of morphine-induced CPP, were considerably decreased by memantine.Conclusion: The present study showed that linalool is able to reduce the acquisition and reinstatement of morphine-induced CPP which might be due tothrough NMDA receptors blocking.
https://ajp.mums.ac.ir/article_8170_42827344d5db9b5b5e30530ff3fd41a7.pdf
Conditioned place preference (CPP)
Linalool
Memantine
Mice
Morphine
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2017-05-01
7
3
250
260
10.22038/ajp.2017.8032
8032
Protective effect of Tribulus terrestris fruit extract on cerulein-induced acute pancreatitis in mice
Mina Borran
mina.borran@gmail.com
1
Mohsen Minaiyan
minaiyan@pharm.mui.ac.ir
2
Behzad Zolfaghari
zolfaghari@pharm.mui.ac.ir
3
Parvin Mahzuni
mahzouni@med.mui.ac.ir
4
School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Clinical Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Objective: Antioxidant, anti-inflammatory, analgesic and antimicrobial activities of Tribulus terrestris (T. terrestris) could be helpful in the treatment of acute pancreatitis; thus, this study was designed to investigate the effects of T. terrestris on cerulein-induced acute pancreatitis in mice. Materials and Methods: Three doses (100, 200 and 400 mg/kg) of T. terrestris hydro-alcoholic extract were administered both orally (60 minutes before pancreatitis induction, p.o.) and intra-peritoneally (30 minutes before pancreatitis induction, i.p.) to different groups of mice (n=6). Pancreatitis was induced by five injections (i.p.) of cerulein 50μg/kg body weight with 1 hr intervals. Animals were euthanized 5 hr after the last injection of cerulein and tissue injures were assessed biochemically and pathologically. Results: T. terrestris extract 200 and 400mg/kg (p.o.) and T. terrestris extract 400 mg/kg (i.p.) reduced pancreatic tissue myeloperoxidase (MPO) activity and serum amylase and lipase levels and alleviated histological parameters. Conclusion: These data suggest that T. terrestris hydro-alcoholic extract was effective in protecting against experimental acute pancreatitis and possibly the efficacy depends on dose and route of administration.
https://ajp.mums.ac.ir/article_8032_b82e3bd78c9c4d3585f386662cf0681a.pdf
Acute pancreatitis
Inflammation
T.terrestris
Cerulein
Mice
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2017-05-01
7
3
261
274
10.22038/ajp.2017.15516.1617
8195
Stereological investigation of the effect of Elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized rats
Mohammad Dabbaghmanesh
dabbaghm@sums.ac.ir
1
Ali Noorafshan
noora@sums.ac.ir
2
Pedram Talezadeh
ptalezadeh@yahoo.com
3
Nader Tanideh
tanidehn@sums.ac.ir
4
Farhad Koohpeyma
farhad_biologist@yahoo.com
5
Aida Iraji
iraji@sums.ac.ir
6
Marzieh Bakhshayeshkaram
bakhshayesh@sums.ac.ir
7
Nima Montazeri-Najafabady
montazerin@sums.ac.ir
8
Department of Endocrinology, Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Anatomy, Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Endocrinology, Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of pharmacology, Stem Cell and Transgenic Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Endocrinology, Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Researcher in Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran
Researcher in Health Policy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Endocrinology, Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Objective: Postmenopausal osteoporosis is characterized by increased fracture risk. However, each approved treatment has specific side effects. Therefore, foods with plant origins have increasingly attracted attention as an alternative treatment. Studies have shown that Elaeagnus angustifolia (EA) has antioxidant properties. The present study aimed to investigate the effects of EA hydroalcoholic extract on ovariectomy-induced osteoporosis in rats using stereological methods. Material and Methods: 55 female Sprague-Dawley rats were randomly assigned to control, sham operated (normal saline), ovariectomized (OVX), OVX + EA fruit extract (600 mg/kg BW/day), and OVX + estradiol benzoate (3 mg/kg BW) for 16 weeks. Blood samples were collected to measure calcium, phosphorus, and alkaline phosphatase (ALP) plasma levels. Then, specimens from tibia and fifth lumbar vertebra (L5) bones were prepared and stereological analysis was done. Results: Ovariectomy significantly decreased the calcium level and increased the ALP level in the OVX group. In spite of improvement in calcium hemostasis in groups treated with estrogen and EA fruit extract (p<0.05), only treatment with estrogen was able to reduce ALP levels. Moreover, treatment with EA fruit extract and estrogen caused a significant increase in the number of osteoblasts in vertebra and tibia compared to the OVX group (p<0.05). Estrogen and EA fruit extract were also able to reduce the number of osteoclasts in tibia of the treated OVX rats (p<0.05). Conclusion: The results showed that EA extract exerted more effects, markedly, on osteoblastogenesis in the OVX rats. Thus, it could be considered as a potential agent to treat patients with osteoporosis.
https://ajp.mums.ac.ir/article_8195_a8570fc32c8c44ac6c659bd7839ddd77.pdf
Elaeagnus angustifolia
Stereology
Osteoporosis
Tibia bone
ALP
Rat
eng
Mashhad University of Medical Sciences
Avicenna Journal of Phytomedicine
2228-7930
2228-7949
2017-05-01
7
3
275
284
10.22038/ajp.2016.16700.1641
8039
Antiplasmodial activity and cytotoxicity of ethanol extract of Zea mays root
Jude Efiom Okokon
judeefiom@yahoo.com
1
Bassey Sunday Antia
antiabassey@yahoo.com
2
Bala Adamu Azare
azabala@yahoo.com
3
Patience Jude Okokon
okokonp@yahoo.com
4
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria
Department of Chemistry, University of Uyo, Uyo, Nigeria
Department of Zoology, University of Abuja, Abuja, Nigeria
Department of Clinical Pharmacy and Biopharmacy, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria
Objective:Zea mays root decoction that has been traditionally used for the treatment of malaria by various tribes in Nigeria, was evaluated for antimalarial potential against malaria parasites using in vivo and in vitro models. Materials and Methods: The root extract of Zea mays was investigated for antimalarial activity against Plasmodium berghei in mice using rodent malaria models; suppressive, prophylactic and curative tests and in vitro antiplasmodial activity against chloroquine-sensitive (Pf 3D7) and resistant (Pf INDO) strains of Plasmodium falciparum using SYBR green assay method. Median lethal dose and cytotoxic activity against HeLa and HEKS cells were assessed and phytochemical screening was also carried out using standard procedures. Results: The LD50 value of root extract was found to be 474.34 mg/kg. The crude extract (45-135 mg/kg, p.o) showed significant (p<0.05-0.001) antimalarial activity against P. berghei infection in suppressive, prophylactic and curative tests with a prolonged survival time. The crude extract also showed moderate activity against both chloroquine-sensitive (Pf 3D7) and resistant (Pf INDO) strains of P. falciparum with an IC50 value of 71.62±3.38 μg/ml (for Pf 3D7) and 63.76±4.12 μg/ml (for Pf INDO). The crude extract was not cytotoxic to the two cell lines tested with TC50 of >100 μg/ml against both HeLa and HEKS cell lines. Conclusion: These results suggest that the root extract of Zea mays possesses antimalarial activity against both chloroquine-sensitive and resistant malaria and these data justify its use in ethnomedicine to treat malaria infections.
https://ajp.mums.ac.ir/article_8039_4c4d3d285686518ac1a1179164c05ae4.pdf
Antimalarial
Antiplasmodial
Plasmodium falciparum
P. berghei
Zea mays