@article { author = {Ghaffari-Nasab, Arshad and Mirzaei Bavil, Fariba and Ghiasi, Rafigheh and Sadigh-Eteghad, Saeed and Alipour, Mohammad Reza}, title = {Effects of IMOD™ on angiogenesis, miR-503 and CDC25 expression levels in heart tissue of diabetic male rats}, journal = {Avicenna Journal of Phytomedicine}, volume = {8}, number = {2}, pages = {152-160}, year = {2018}, publisher = {Mashhad University of Medical Sciences}, issn = {2228-7930}, eissn = {2228-7949}, doi = {10.22038/ajp.2017.23107.1856}, abstract = {Objective: Diabetes is associated with vascular complications and impaired angiogenesis. Since angiogenesis plays a crucial role in vascular homeostasis in ischemic heart diseases, in this study, the effect of IMOD™ on miR-503 and CDC25 expression level which are altered in impaired angiogenesis were investigated in heart tissue of diabetic rats. Materials and Methods: Forty male Wistar rats (200-250 g) were randomly classified into 4 groups: control (C), IMOD™ (I), diabetes (D), and diabetes+IMOD™ (D+I). For induction of experimental diabetes in animals, a single dose of streptozotocin (STZ; 60mg/kg) was injected intraperitoneally. After 8 weeks of treatment with IMOD™ (20 mg/kg/day), heart tissue samples were removed and used for measurement of miR-503 and CDC25 expression level as well as histological studies. Results: Results of this study showed that diabetes decreased heart tissue angiogenesis which was associated with increased miR-503 and reduced CDC25 expression levels (p<0.05) and IMOD™ could reduce the expression of miR-503 and increase the expression of CDC25 (p<0.05). Moreover, IMOD™ extensively induced angiogenesis in the heart tissue of diabetic group. However, IMOD™ had no significant effect on expressions of miR-503 and CDC25, or angiogenesis in healthy rats. Conclusion: This study showed that IMOD™ is able to increase angiogenesis in the heart tissue of diabetic rats. The angiogenic effect of IMOD™ is associated with reduction of miR-503 expression and increased expression of CDC25.}, keywords = {Diabetes,Angiogenesis,miR-503,CDC25,IMOD}, url = {https://ajp.mums.ac.ir/article_9682.html}, eprint = {https://ajp.mums.ac.ir/article_9682_8369912f1f26b0033094a7442ff8ef61.pdf} }