@article { author = {Shafiee-Nick, Reza and Parizadeh, Seyyed Mohammad Reza and Zokaei, Nona and Ghorbani, Ahmad}, title = {Effect of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets}, journal = {Avicenna Journal of Phytomedicine}, volume = {2}, number = {4}, pages = {206-211}, year = {2012}, publisher = {Mashhad University of Medical Sciences}, issn = {2228-7930}, eissn = {2228-7949}, doi = {10.22038/ajp.2012.110}, abstract = {Objective: Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level.This study was undertaken to investigateits direct effect on the islets of Langerhans. Material and Methods: Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer containing 3 or 10 mM glucose in the presence of hydroalcoholic extract of G. Lucidum (1 mg/ml), 3-isobutyl-1-methylxanthine (IBMX, 100 µM) or vehicle. Results: Exposure of islets to the extract increased insulin secretion at basal (3 mM) glucose concentration. Increase of glucose concentration to 10 mM resulted in a significant increase in the rate of insulin secretion. While the IBMX could augment insulin release evoked by 10 mM glucose, the extract failed to modify it. Conclusion: Our results demonstrate that G. lucidum acts directly on the Langerhans islets to increase basal insulin release. }, keywords = {Ganoderma lucidum,Insulin,Isolated islets,Rat}, url = {https://ajp.mums.ac.ir/article_110.html}, eprint = {https://ajp.mums.ac.ir/article_110_43205452fee19c76288153ff9fe01359.pdf} }