Gustav III’s risk assessment on coffee consumption; A medical history report
Reza
Afshari
Environmental Health Services, BC Centre for Disease Control, Vancouver, Canada
author
text
article
2017
eng
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
7
v.
2
no.
2017
99
100
https://ajp.mums.ac.ir/article_7870_20a0972baf06cd217911e655328d64c8.pdf
dx.doi.org/10.22038/ajp.2016.7870
In vitro and in vivo assessment of inhibitory effect of stevioside on pro-inflammatory cytokines
Jatuporn
Noosud
Department of Companion Animal Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand
author
Narissara
Lailerd
Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
author
Autchara
Kayan
Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand
author
Chaiwat
Boonkaewwan
Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand
author
text
article
2017
eng
Objective: Stevioside is a natural non-caloric sweetener which has been reported to have anti-inflammatory activity. The aim of the present study was to examine in vitro and in vivo effects of stevioside on rats plasma levels of tumor necrosis factor- α (TNF-α), interleukin-1β (IL-1β), TNF-α and IL-1β release from lipopolysaccharide(LPS)-stimulated rat peripheral blood mononuclear cells (PBMCs). Materials and Methods: Male wistar rats weighing between 170-220 g were given stevioside (0, 500 and 1000 mg/kg BW/day) for 6 weeks. Mononuclear cells were separated from peripheral blood samples. TNF-α and IL-1β levels in plasma and the release of TNF-α and IL-1β from PBMCs were determined using rat enzyme-linked immunosorbent assay (ELISA) kits. Results: Plasma levels of TNF-α and IL-1β were found to be non-detectable in control and groups treated with 500 and 1000 mg/kg of stevioside. Regarding TNF-α release from LPS-stimulated PBMCs, rats that were orally fed with 500 and 1000 mg/kg of stevioside were significantly different (p<0.05) from those in LPS-treated control group (186.8+18.6 and 151.4 + 15.4 vs 248.6+21.4 pg/ml). Additionally, IL-1β levels in rats treated with 500 and 1000 mg/kg of stevioside were significantly different (p<0.05) from those in LPS-treated control group (220.0+12.1 and 158.1 + 22.6 vs 294.4+16.1 pg/ml). Conclusion: Consumption of stevioside has an inhibitory effect on the release of TNF-α and IL-1β from LPS-stimulated PBMCs in rats.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
7
v.
2
no.
2017
101
106
https://ajp.mums.ac.ir/article_6599_ccd21beb18f0196952f100e27acd74e4.pdf
dx.doi.org/10.22038/ajp.2016.6599
Cassia fistula: A remedy from Traditional Persian Medicine for treatment of cutaneous lesions of pemphigus vulgaris
Fatemh
Atarzadeh
Department of Traditional Iranian Medicine, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Amir
Jaladat
Department of Traditional Persian Medicine, School of Medicine, Shiraz, Iran
author
Ladan
Dastgheib
Molecular Dermatology Research Center, Department of Dermatology, Shiraz University of Medical Sciences, Shiraz, Iran
author
Gholamreza
Amin
Department of Traditional Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
author
Majid
Nimrouzi
Department of Traditional Persian Medicine, School of Medicine, Shiraz, Iran
author
Mohammad
Kamalinejad
Department of Pharmacognosy, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran
author
text
article
2017
eng
Objective: Pemphigus is a rare autoimmune disease that may be fatal without proper medical intervention. It is a blistering disease that involves both the skin and mucus membranes, in which the most important causes of death comprise superimposed opportunistic infections and complications of long-term high-dose corticosteroid therapy or prolonged consumption of immune suppressant drugs. Skin lesions are the most important sources of infection, and any local treatment decreasing the healing time of lesions and reducing the total dosage of drugs is favorable. Materials and Methods: Here, we review the probable mechanism of action of a traditional formulary of Cassia fistula (C. fistula) fruit extract in almond oil as a new topical medication for reducing the duration of treatment of pemphigus vulgaris erosions. Result: C. fistula fruit oil has lupeol, anthraquinone compounds as rhein and flavonoids. Previous in vitro and animal studies on C. fistula fruit have demonstrated wound healing, antioxidative, anti-leukotrienes, anti-inflammatory, antibacterial and antifungal effects of this plant. Conclusion: It is hypothesized that C. fistula L. can be a botanical therapeutic choice for treatment of pemphigus erosions.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
7
v.
2
no.
2017
107
115
https://ajp.mums.ac.ir/article_6629_3b5f91813f5cb87dbf8f3d847fe9abe1.pdf
dx.doi.org/10.22038/ajp.2016.6629
Preventive effect of Coriandrum sativum on neuronal damages in pentylentetrazole-induced seizure in rats
Mojtaba
Pourzaki
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mansour
Homayoun
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Saeed
Sadeghi
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Masoumeh
seghatoleslam
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Microanatomy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
author
Mahmoud
Hosseini
Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Alireza
Ebrahimzadeh Bideskan
Department of Anatomy and Cell Biology, Microanatomy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2017
eng
Objective:Coriandrum sativum (C. sativum) as a medicinal plant has been pointed to have analgesic, hypnotic and anti-oxidant effects. In the current study, a possible preventive effect of the hydro-alcoholic extract of the plant on neuronal damages was examined in pentylenetetrazole (PTZ) rat model of seizure.Materials and Methods: Forty male rats were divided into five main groups and treated by (1) saline, (2) PTZ: 100 mg/kg PTZ (i.p) and (3-5) 50, 100 and 200 mg/kg of hydro-alcoholic extract of C. sativum during seven consecutive days before PTZ injection. After electrocorticography (ECoG), the brains were removed to use for histological examination.Results: All doses of the extract reduced duration, frequency and amplitude of the burst discharges while prolonged the latency of the seizure attacks (p<0.05, p<0.01, and p<0.001). Administration of all 3 doses of the extract significantly prevented from production of dark neurons (p<0.01, and p<0.001) and apoptotic cells (p<0.05, p<0.01, and p<0.001) in different areas of the hippocampus compared to PTZ group.Conclusion: The results of this study allow us to conclude that C. sativum,because of its antioxidant properties, prevents from neuronal damages in PTZ rat model of seizure.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
7
v.
2
no.
2017
116
128
https://ajp.mums.ac.ir/article_7757_06062e445614ed2f7d8ad7a3d39cf88c.pdf
dx.doi.org/10.22038/ajp.2016.7757
A comparison of the efficacy of metronidazole vaginal gel and Myrtus (Myrtus communis) extract combination and metronidazole vaginal gel alone in the treatment of recurrent bacterial vaginosis
Mansoure
Masoudi
Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
author
Mahmoud
Rafieian
Medicinal Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
author
Sepide
Miraj
Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
author
text
article
2017
eng
Objective: Due to the high incidence of bacterial vaginosis (BV) and its resistance to chemical medications and considering the anti-bacterial and anti-fungal effects of Myrtus communis, the present study aimed to compare the therapeutic effects of the vaginal gel of M. communis 2% (in metronidazole base) with metronidazole vaginal gel 0.75% alone on BV.Materials and Methods: This research was a randomized controlled clinical trial conducted on 80 women of 18-40 years old with BV. Patients were divided into two groups of 40 women. Diagnostic criteria were Amsel's criteria and Gram staining. The first group received vaginal gel of metronidazole plus M. communis 2% and the second group received metronidazole vaginal gel alone for five consecutive nights. Therapeutic effects and Amsel’s criteria were assessed after one week. Finally, the data were analyzed by SPSS 16 using t-test and Chi square tests.Results: There was a significant difference in the therapeutic response between the two groups. The results demonstrated that the combination of metronidazole and M. communis had a higher efficiency (p<0.05). The patients receiving M. communis in metronidazole gel base did not experience any recurrent BV, but 30% of patients taking metronidazole alone faced recurrent BV after three weeks of follow up.Conclusion: Findings of the study suggested that adding M. communis extract to metronidazole increases the efficiency of BV treatment.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
7
v.
2
no.
2017
129
136
https://ajp.mums.ac.ir/article_7286_1bfc4e3f99077ce92b410a986f10a0af.pdf
dx.doi.org/10.22038/ajp.2016.7286
Kelussia odoratissima potentiates cytotoxic effects of radiation in HeLa cancer cell line
Azar
Hosseini
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Shima
Saeidi Javadi
Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Azar
Fani Pakdel
Department of Oncology, Omid Hospitsal, Mashhad, Iran
author
Seyed Hadi
Mousavi
Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2017
eng
Objective: Cervical cancer is the second most common cause of death from cancer in women throughout the world. The aim of this study was to evaluate the cytotoxic activity of Kelussia odoratissima (K. odoratissima) extract associated with radiotherapy in cervical cancer cells (HeLa cell line).Materials and Methods: Different concentration of the extract (25-500µg/ml) was tested in HeLa cell lines. Cell cytotoxicity of the extract and the effects of the extract on radiation (2Gy/min)-induced damages were assessed by MTT assay. Apoptosis was assessed using flow cytometric analysis.Result: K. odoratissima decreased cell viability in HeLa cell line in a concentration and time-dependent manner. When compared to the control,K. odoratissima induced a sub-G1 peak in the flow cytometry histogram of treated cells, indicating that apoptotic cell death is involved in K. odoratissima-induced toxicity. It was also shown that K. odoratissima sensitizes cells to radiation-induced toxicity.Conclusion: Our result showed the extract increased the radiation effect. This observation may be related to the presence of active compounds such as phthalides and ferulic acid.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
7
v.
2
no.
2017
137
144
https://ajp.mums.ac.ir/article_7759_369738cc6cc78a4f3cabae713fffd7fd.pdf
dx.doi.org/10.22038/ajp.2016.7759
Anti-carcinogenic and anti-angiogenic properties of the extracts of Acorus calamus on gastric cancer cells
Samaneh
Rahamoz Haghighi
Department of Plant Breeding, Faculty of New Sciences and Technologies, Graduate University of Advanced Technology, Kerman, Iran
author
Malek Hossein
Asadi
Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
author
Hassan
Akrami
Department of Biology, Faculty of Basic Science, Razi University, Kermanshah, Iran
author
Amin
Baghizadeh
Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
author
text
article
2017
eng
Objective: Acorus calamus (A. calamus) has been used as a medicinal plant in Asia for its effects on digestive system for the last 2000 years. To investigate the anti-cancer activity of rhizome of A. calamus, the ethanolic and methanolic extracts and essential oil of the rhizome were prepared and their effects were assessed on human gastric cancer cell line (AGS). Materials and Methods: The viability of cells which were treated with the extracts and the essential oil was assessed by MTT assay. To evaluate the anti-angiogenic property of the extracts, in vitro tube formation assay was done. Cell cycle distribution and the expression of Oct4 and Nucleostemin, after treatments, were checked by flowcytometry and quantitative RT-PCR, respectively. Furthermore, analysis of essential oil from A.calamus was done by GC-MS. Results: Our results showed that the growth of AGS cells was inhibited by the extracts and essential oil and the extracts inhibited the angiogenesis in HUVEC cells. Our data revealed that the extracts and essential oil of A. calamus caused G1 arrest in AGS cells and downregulation of Oct4 and NS after treatment. By GC-MS analysis, we found new compoundssuch as epiprezizaene, valencene and isocyclocitral in essential oil of A. Conclusions: All together, our results showed that the extracts of A. calamus have anti-proliferative and anti-angiogenic effects on cancer cells.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
7
v.
2
no.
2017
145
156
https://ajp.mums.ac.ir/article_7485_649faba38a889b3c2d7b98ad8af98cb8.pdf
dx.doi.org/10.22038/ajp.2016.7485
Antioxidant, cytotoxic and DNA protective properties of Achillea eriophora DC. and Achillea biebersteinii Afan. extracts: A comparative study
Maryam
Varasteh-kojourian
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
author
Parvaneh
Abrishamchi
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
author
Maryam
Matin
Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
author
Javad
Asili
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Hamid
Ejtehadi
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
author
Fatemeh
Khosravitabar
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
author
text
article
2017
eng
Objective: Achillea is a traditional medicinal herb which contains different phenol and flavonoid compounds that are responsible for Achillea pharmacological effects. We aimed to determine phenol and flavonoid contents, besides antioxidant activities of different extracts from Achillea eriophoraa (A. eriophora) DC. and Achillea biebersteinii (A. biebersteinii) Afan. (endemic species in Iran) and to investigate their effects on human cells.Materials and Methods: Achillea extracts, were prepared by maceration and shaking methods, from different parts (aerial parts, stem, leaves and inflorescence) of two species using methanol and ethanol as solvents. Total phenol and flavonoid contents were measured by spectrophotometry, and antioxidant activities of the extracts were determined by DPPH radical scavenging, BCB and TBARS assays. Cytotoxicity and antioxidant activities of the extracts were investigated in Human Foreskin Fibroblast (HFF3) cells using MTT, comet and H2O2 assays.Results: Methanol extracts of A. biebersteinii prepared from leaves and inflorescence by maceration method exhibited maximum phenol (1657.58 ± 36.45 mg GAE/100 g DW) and flavonoid (264.00 ± 62.16 mg QUE/100 g DW) contents. Leaf methanol extract showed significantly higher antioxidant activity (0.0276 ± 0.003, 0.16 ± 0.016 and 13.96 ± 0.26 mg/ml for DPPH, BCB and TBARS IC50s, respectively) than those of the other extracts. Leaf extract of A. biebersteinii was not cytotoxic even at the highest examined dose (512 µg/ml) and inhibited cell toxicity induced by H2O2 (98% viability for the cells pretreated with plant extract in the presence of H2O2). Comet assay also confirmed high DNA protective activity of leaf extracts.Conclusion: Achillea extracts possess remarkable antioxidant activity, and could be good natural alternatives to synthetic antioxidants in pharmaceutical and food industries.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
7
v.
2
no.
2017
157
168
https://ajp.mums.ac.ir/article_7756_d9047c55aa95180b095645e2ea2c86e0.pdf
dx.doi.org/10.22038/ajp.2016.7756
Antidiabetic, hypolipidemic and hepatoprotective effects of Arctium lappa root’s hydro-alcoholic extract on nicotinamide-streptozotocin induced type 2 model of diabetes in male mice
Akram
Ahangarpour
Health Research Institute, Diabetes Research Center, Department of Physiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
Hamid
Heidari
Department of Physiology, Health Research Institute, Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Physiology, School of Medicine, Qom University of Medical Sciences, Qom, Iran
author
Ali Akbar
Oroojan
Department of Physiology, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
Farhang
Mirzavandi
Department of Nutrition, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
Khalil
Nasr Esfehani
Department of Nutrition, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
Zienab
Dehghan Mohammadi
Health Research Institute, Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
text
article
2017
eng
Objective: Arctium lappa (burdock), (A. lappa) root has hypoglycemic and antioxidative effects, and has been used for treatment of diabetes in tradition medicine. This study was conducted to evaluate the antidiabetic and hypolipidemic properties of A. lappa root extract on nicotinamide-streptozotocin (NA-STZ)-induced type2 diabetes in mice.Materials and Methods: In this investigation, 70 adult male NMRI mice (30-35g) randomly divided into 7 groups (n=10) as follow: 1-control, 2-type 2 diabetic mice, 3-diabetic mice that received glibenclamide (0.25 mg/kg) as an anti-diabetic drug, 4, 5, 6 and 7- diabetic and normal animals that were pre-treated with 200 and 300 mg/kg A. lappa root extract, respectively, for 28 days. Diabetes has been induced by intraperitoneal injection of NA and STZ. Finally, the blood sample was taken and insulin, glucose, SGOT, SGPT, alkaline phosphatase, leptin and lipid levels was evaluated.Results: Induction of diabetes decreased the level of insulin, leptin and high density lipoprotein (HDL) and increased the level of other lipids, glucose, and hepatic enzymes significantly (p<0.05). Administration of both doses of the extract significantly decreased the level of triglyceride, very low density lipoprotein, glucose and alkaline phosphatase in diabetic mice (p<0.05). Insulin levels increased in animals treated with 200 mg/kg (p<0.05) and HDL and leptin levels increased in animals treated with 300 mg/kg of the extract (p<0.01).Conclusion: The results showed that A. lappa root extract, at specific doses, has an anti-diabetic effect through its hypolipidemic and insulinotropic properties. Hence, this plant extract may be beneficial in the treatment of diabetes.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
7
v.
2
no.
2017
169
179
https://ajp.mums.ac.ir/article_7843_f33f9f412b79d15636bdcea6bd0ece98.pdf
dx.doi.org/10.22038/ajp.2016.7843
Kidney stone formation and antioxidant effects of Cynodon dactylon decoction in male Wistar rats
Alireza
Golshan
School of Medicine, North Khorasan University of Medical sciences, Bojnurd, Iran
author
Parichehr
Hayatdavoudi
Neurocognitive Research Center, Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mousa AL-Reza
Hadjzadeh
Neurocognitive Research Center, Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Abolfazl
Khajavi Rad
Neurogenic Inflammation Research Center,Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Nema
Mohamadian Roshan
Department of Pathology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhd, Iran
author
Abbasali
Abbasnezhad
Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad,Iran
author
Seyed Mojtaba
Mousavi
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad,Iran
author
Roghayeh
Pakdel
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad,Iran
author
Batool
Zarei
Department of physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Azita
Aghaee
Pharmacological Research Center of Medicinal Plants, Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad,Iran
author
text
article
2017
eng
Objectives: The antioxidant capacity impairs in kidney and urinary bladder of animals with stone disease. Herbal medicine can improve the antioxidant condition of renal tissue. Cynodon dactylon (C. dactylon) is a medicinal plant with antioxidative and diuretic properties and different preparations of this plant have shown promising effects in stone disease. Assessment of the whole plant decoction to prevent kidney stone disease as well as its antioxidant effects was the aim of this paper.Materials and Methods: Fifty male Wistar rats were randomly divided into 5 experimental groups (n=10). One group was left without treatment and four groups received ethylene glycol (1% v/v) in drinking water for 6 weeks. Three doses of Cynodon dactylon aqueous decoction (12.5, 50 and 200 mg/kg BW) were added to the drinking water of groups 3-5. Finally, water intake, 24-hour urine volume, MDA, total thiol concentration and FRAP value were measured in the serum and kidney tissues. The CaOx depositions were evaluated by hematoxylin and eosin staining.Results: Compared to the ethylene glycol-treated group, 200 mg/kg C. dactylon, lowered stone incidents, decreased urine volume, increased FRAP/g Cr (43%) and thiol content (p<0.05) with no significant alteration of water intake, MDA decreased significantly compared to C. dactylon 12.5 (p<0.01). Kidney weight increased and body weight decreased in ethylene glycol-treated group compared to the control group (p<0.05). Conclusion: A minimum dose of 200 mg/kg C. dactylon reduced stone formation and simultaneously increased total antioxidant power of serum and preserved MDA content and water.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
7
v.
2
no.
2017
180
190
https://ajp.mums.ac.ir/article_7844_809ce8160bc420d029e5b9faef8ff333.pdf
dx.doi.org/10.22038/ajp.2016.7844
Capparis spinosa L. aqueous extract evokes antidiabetic effect in streptozotocin-induced diabetic mice
Mohamed
Eddouks
Faculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, Morocco
author
Ahmed
Lemhadri
Faculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, Morocco
author
Morad
Hebi
Faculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, Morocco
author
Ahmed EL
Hidani
Faculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, Morocco
author
Naoufel Ali
Zeggwagh
ISPITS Rabat, Avenue Hassan II Km 4,5 Route de Casa, Rabat Maroc
author
Bachir
EL Bouhali
Faculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, Morocco
author
Lhoussaine
Hajji
Faculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, Morocco
author
Remy
Burcelin
2UMR 5018 CNRS-UPS and IFR 31, Rangueil Hospital, L1 Bldg, BP 84225 Toulouse 31432 Cedex 4, France
author
text
article
2017
eng
Objective: As the aqueous extract of Capparis spinosa (CS) possess antidiabetic effect, he present study aims to reveal the possible mechanism of action of CS in diabetic mice.Materials and Methods: Both single and repeated oral administrations of aqueous extract of CS were performed in multi-low dose streptozotocin-induced (MLDS) diabetic mice. Euglycemic hyperinsulinemic clamp was used in association with the endogenous glucose production (perfusion rate of 3-3H glucose) to evaluate the effect of CS aqueous extract on insulin sensitivity.Results: Our study showed that aqueous extract of CS possess a potent hypoglycaemic activity in MLDS diabetic mice. Furthermore, the analysis perfusion of 3-3H glucose demonstrated the parallel decrease of basal endogenous glucose production (EGP) with the hypoglycaemic activity. EGP was lower in CS-Treated group when compared to the control group (p<0.001). The euglycemic hyperinsulinemic clamp technique demonstrated that CS treatment improves insulin sensitivity in peripheral tissues.Conclusion: We conclude that the antihyperglycemic effet CS is probably due to the inhibition of basal endogenous glucose production and the improvement of insulin sensitivity in MLDS diabetic mice.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
7
v.
2
no.
2017
191
198
https://ajp.mums.ac.ir/article_7761_b203ca4b3cb6490d2d1155e2946bd6b4.pdf
dx.doi.org/10.22038/ajp.2016.7761