Effect of methanol leaf extract of Dalbergia saxatilis Hook.f (fabaceae) on renal function
Fatima Ismail
Hassan
Department of Pharmacology and Therapeutics, Ahmadu Bello University, Zaria, Nigeria
author
Abdulkadir Umar
Zezi
Department of Pharmacology and Therapeutics, Ahmadu Bello University, Zaria, Nigeria
author
Umar Habib
Danmalam
Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria, Nigeria
author
Abdullahi Hamza
Yaro
Department of Pharmacology, Bayero University, Kano, Nigeria
author
text
article
2016
eng
Objective: Dalbegia saxatilis (D.saxatilis) is used as a decoction in traditional medicine for ailments such as cough, small pox, skin lesions, bronchial ailments and toothache. This study is aimed at evaluating the toxic effect of methanol leaf extract of D.saxatilis on renal function. Materials and Methods: Wistar rats of both sexes were divided into four groups of five: control animals (group 1) received distilled water 1 ml/kg while groups 2, 3 and 4 were given graded doses of the extract (250, 500 and 1000 mg/kg body weight, respectively) daily for 28 days. Body weight changes were estimated by weighing the rats twice weekly using digital weighing balance. After 28 days, blood samples were obtained for evaluation of renal indices and the kidney was used for histopathology. Data were analysed using one–way and repeated measures ANOVA using SPSS version 20. Results: Significant weight increase in all groups were observed (p<0.05). Significant reduction in electrolytes concentration was observed following treatment with extract (250 and 500 mg/kg) (p<0.05). Histopathological findings of the kidney revealed massive necrosis of the glomerulus with tubular distortion and lymphocyte hyperplasia at 250 and 500 mg/kg while intense glomerular and tubular necrosis was observed at 1000 mg/kg of the extract. Conclusion: Since different doses of the extract caused reduction in electrolyte concentration and damage to the kidney it is suggested that prolonged administration of the extract is associated with increased risk of kidney toxicity.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
6
v.
6
no.
2016
592
596
https://ajp.mums.ac.ir/article_6321_ff8f74f4aec414611283dbca2ecf2156.pdf
dx.doi.org/10.22038/ajp.2016.6321
The effect of Zataria multiflora Boiss hydroalcoholic extract and fractions in pentylenetetrazole-induced kindling in mice
Ali
Shamsizadeh
Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Farangis
Fatehi
Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Fatemeh
Arab Baniasad
Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Fatemeh
Ayoobi
Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
author
Mohammad Ebrahim
Rezvani
Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
author
Ali
Roohbakhsh
Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2016
eng
Objective: At present, there are many antiepileptic drugs with a wide range of side effects on the human body. It was assumed that Zataria multiflora Boiss (Z. multiflora) with sedative, anti-spasmodic and anti-inflammatory activity may be effective in the treatment of epilepsy. The aim of the present study was to elucidate the effect of Z. multiflora hydroalcoholic extract and its fraction extracts on pentylenetetrazole (PTZ)-induced chemical kindling. Materials and Methods: In this experimental study, eight separate groups of male albino mice were used. All groups received 11 separate intraperitoneal injections of PTZ (35 mg/kg) with two-day intervals. 30 min before the injection of PTZ, mice received vehicle, Z. multiflora hydroalcoholic extract (300 and 600 mg/kg), n-hexane, acetone, methanol fraction extracts (150 mg/kg), or diazepam (10 mg/kg). Results: The kindled mice that were pretreated with vehicle showed a gradual increase in their seizure scores up to the end of the study. The hydroalcoholic extract of Z. multiflora (300 and 600 mg/kg) reduced seizure scores significantly. However, n-hexane, acetone and methanol extracts did not affect seizure scores significantly. Conclusion: The present findings demonstrate that the hydroalcoholic extract of Z. multiflora did reduce the severity of seizure attacks in PTZ-induced chemical kindling in mice.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
6
v.
6
no.
2016
597
603
https://ajp.mums.ac.ir/article_6328_6146d78a89a6838b26ba7e792c37d7b1.pdf
dx.doi.org/10.22038/ajp.2016.6328
Saffron’omics’: The challenges of integrating omic technologies
Sameera
Panchangam
Bioclues.org, Kukatpally, Hyderabad 500072, Telangana, India
author
Maryam
Vahedi
Department of Horticultural Science, Faculty of Agricultural Sciences and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj 4111, Iran
author
MohanKumar
Megha
Bioclues.org, Kukatpally, Hyderabad 500072, Telangana, India
author
Anuj
Kumar
Advanced Center for Computational & Applied Biotechnology, Uttarakhand Council for Biotechnology, Dehradun 248007, India
author
Kaamini
Raithatha
Bioclues.org, Kukatpally, Hyderabad 500072, Telangana, India
and
Department of Applied Mathematics, The Maharaja Sayajirao University of Baroda 390002, Gujarat
author
Prashanth
Suravajhala
Bioclues.org, Kukatpally, Hyderabad 500072, Telangana, India
author
Pratap
Reddy
Bioclues.org, Kukatpally, Hyderabad 500072, Telangana, India
author
text
article
2016
eng
Saffron is one of the highly exotic spices known for traditional values and antiquity. It is used for home décor besides serving as a colorant flavor and is widely known for medicinal value. Over the last few years, saffron has garnered a lot of interest due to its anti-cancer, anti-mutagenic, anti-oxidant and immunomodulatory properties. Integration of systems biology approaches with wide applications of saffron remains a growing challenge as new techniques and methods advance. Keeping in view of the dearth of a review summarizing the omics and systems biology of saffron, we bring an outline on advancements in integrating omic technologies, the medicinal plant has seen in recent times.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
6
v.
6
no.
2016
604
620
https://ajp.mums.ac.ir/article_6792_be0f0008e4f6a75372063f5268d2f0a7.pdf
dx.doi.org/10.22038/ajp.2016.6792
Ethnobotanical knowledge of Apiaceae family in Iran: A review
Mohammad Sadegh
Amiri
Department of Biology, Payame Noor University, Tehran, Iran
author
Mohammad Reza
Joharchi
Department of Botany, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
author
text
article
2016
eng
Objective: Apiaceae (Umbelliferae) family is one of the biggest plant families on the earth. Iran has a huge diversity of Apiaceae members . This family possesses a range of compounds that have many biological activities. The members of this family are well known as vegetables, culinary and medicinal plants. Here, we present a review of ethnobotanical uses of Apiaceae plants by the Iranian people in order to provide a comprehensive documentation for future investigations. Materials and Methods: We checked scientific studies published in books and journals in various electronic databases (Medline, PubMed, Science Direct, Scopus and Google Scholar websites) from 1937 to 2015 and reviewed a total of 52 publications that provided information about different applications of these plant species in human and livestock. Results: As a result of this review, several ethnobotanical usages of 70 taxa, 17 of which were endemic, have been determined. These plants were used for medicinal and non-medicinal purposes. The most commonly used parts were fruits, leaves, aerial parts and gums. The most common methods of preparation were decoction, infusion and poultice. Conclusion: To our knowledge, this paper represents a comprehensive literature search of ethnobotanical uses of Apiaceae reported from Iran. This study highlights the rich traditional knowledge of this family that has remained in Iran. However, most of this knowledge survive only as memories from the past in the minds of the elderly, and will probably vanish in a few decades. Thus, we compiled these scattered data together in a single document for the next scientific works with ethnobotanical interests.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
6
v.
6
no.
2016
621
635
https://ajp.mums.ac.ir/article_6696_5d38bf408c379558cf57d096d3ce4ade.pdf
dx.doi.org/10.22038/ajp.2016.6696
Carvacrol attenuates serum levels of total protein, phospholipase A2 and histamine in asthmatic guinea pig
Mohammad Hossein
Boskabady
Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Sedigheh
Jalali
Department of Biology, Payam Noor University, 19395-4697 Tehran, Iran
author
Negin
Yahyazadeh
Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mostafa
Boskabady
Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2016
eng
Objective: Pharmacological effects of carvacrol such as its anti-inflammatory activities have been shows. In this study the effects of carvacrol on serum levels of total protein (TP), phospholipase A2 (PLA2) and histamine in sensitized guinea pigs was evaluated. Materials and Methods: Sensitized guinea pigs were given drinking water alone (group S), drinking water containing three concentrations of carvacrol (40, 80 and 160 µg/ml) or dexamethasone. Serum levels of TP, PLA2 and histamine were examined I all sensitized groups as well as a non-sensitized control group (n=6 for each group). Results: In sensitized animals, serum levels of TP, PLA2 and histamine were significantly increased compared to control animals (p<0.05 to p<0.001). Significant reduction in TP, PLA2 and histamine levels were observed in treated groups with the two higher concentrations of carvacrol but dexamethasone treatment only decreased serum level of PLA2 (p<0.001). Although the effect of the lowest concentration of the extract was less than that of dexamethasone (p<0.05 for TP and p<0.001 for PLA2), the effects of the two higher concentrations on PLA2 were similar to dexamethasone and on TP (p<0.01) and histamine (p<0.001) were higher than those of dexamethasone. Conclusion: These results showed that carvacrol reduced serum levels of TP, PLA2 and histamine in sensitized guinea pigs which may indicate an anti-inflammatory effect of this agent in inflammatory disorders such as asthma.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
6
v.
6
no.
2016
636
642
https://ajp.mums.ac.ir/article_7762_86ed350f74e2c59f62374d73a7bdef6f.pdf
dx.doi.org/10.22038/ajp.2016.7762
The effects of cinnamaldehyde and eugenol on human adipose-derived mesenchymal stem cells viability, growth and differentiation: a cheminformatics and in vitro study
Abdorrahim
Absalan
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Seyed Alireza
Mesbah-Namin
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Taki
Tiraihi
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Taher
Taheri
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
author
text
article
2016
eng
Objective: The aim of this study was to estimate the cheminformatics and qualitative structure-activity relationship (QSAR) of cinnamaldehyde and eugenol. The effects of cinnamaldehyde and eugenol on the viability, doubling time and adipogenic or osteogenic differentiations of human adipose-derived mesenchymal stem cells (hASCs) were also investigated. Materials and Methods: QSAR and toxicity indices of cinnamaldehyde and eugenol were evaluated using cheminformatics tools including Toxtree and Toxicity Estimation Software Tool (T.E.S.T) and molinspiration server. Besides, their effects on the hASCs viability, doubling time and differentiation to adipogenic or osteogenic lineages were evaluated. Results: Cinnamaldehyde is predicted to be more lipophilic and less toxic than eugenol. Both phytochemicals may be developmental toxicants. They probably undergo hydroxylation and epoxidation reactions by cytochrome-P450. The 2.5 µM/ml cinnamaldehyde and 0.1 µg/ml eugenol did not influence hASCs viability following 72 hr of treatment. But higher concentrations of these phytochemicals insignificantly increased hASCs doubling time till 96 hr, except 1 µg/ml eugenol for which the increase was significant. Only low concentrations of both phytochemicals were tested for their effects on the hASCs differentiation. The 2.5 µM/ml cinnamaldehyde and 0.1 µg/ml eugenol enhanced the osteogenesis and decreased the adipogenesis of hASCs meaningfully. Conclusion: According to the cheminformatics analysis and in vitro study, cinnamaldehyde and eugenol are biocompatible and low toxic for hASCs. Both phytochemicals may be suitable for regenerative medicine and tissue engineering when used at low concentrations, but maybe useful for neoplastic growth inhibition when used at high concentrations.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
6
v.
6
no.
2016
643
657
https://ajp.mums.ac.ir/article_6785_24c9a635f2367ed15c596d23d2d562d2.pdf
dx.doi.org/10.22038/ajp.2016.6785
The effects of crocin, insulin and their co-administration on the heart function and pathology in streptozotocin-induced diabetic rats
Amir
Farshid
Division of Pathology, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Esmaeal
Tamaddonfard
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Masoumeh
Moradi-Arzloo
Division of Pathology, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Navideh
Mirzakhani
Division of Pathology, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2016
eng
Objective: Crocinisa saffron constituent with a potent anti-oxidant activity. The present study investigated the effects of crocin and insulin treatments (alone or in combination) on cardiac function and pathology in diabetic rats. Materials and Methods: Diabetes was induced by intraperitoneal (i.p.) injection of streptozotocin (STZ, 50 mg/kg). Thereafter, crocin (5, 10 and 20 mg/kg, i.p.), subcutaneous (s.c.) injection of insulin (4 IU/kg) and their combination were administeredfor eight weeks. Blood glucose level andwhole heart and body weights were measured. Electrocardiography (ECG) was carried out using the lead II. Serum concentrations of lactate dehydrogenase (LDH), creatine kinase-MB isoenzyme (CK-MB), and the heart tissue malodialdehyde (MDA) and superoxide dismutase (SOD) contents were determined. The heart lesions were evaluated by light microscopy. Results: STZ decreased body weight and increased whole heart weight/body weight ratio. It also decreased heart rate, and increased RR and QT intervals and T wave amplitude. STZ increased blood glucose, serum LDH andCK-MB levels, augmentedheart tissue MDA content, decreased SOD content of heart tissue, and produced hemorrhages, degeneration, interstitial edema, and fibroblastic proliferation in the heart tissue. Crocin (10 and 20 mg/kg, i.p.), insulin (4 IU/kg, s.c.) and their combination (5 mg/kg of crocin with 4 IU/kg of insulin) treatments recovered the ECG, biochemical and histopathological changes induced by STZ. Conclusion: The results showed cardioprotective effects of crocin and insulin in STZ-induced diabetic rats. The antioxidant and anti-hyperglycemic properties of crocin and insulin may be involved in their cardioprotective actions.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
6
v.
6
no.
2016
658
670
https://ajp.mums.ac.ir/article_6775_9e8e8d48624e92247dc4a2ccd07ebb78.pdf
dx.doi.org/10.22038/ajp.2016.6775
Effects of Biebersteinia multifida hydro-ethanol extract on proliferation and apoptosis of human prostate cancer and human embryonic kidney cells
Alireza
Golshan
Medicinal Plants & Natural Products Research Center (MPNPRC), North Khorasan University of Medical Sciences, Bojnurd, Iran
author
Samira
Hassanzadeh
Pharmacological Research Centre of Medicinal Plants, School of Medicine, Mashhad, University of Medical Sciences, Mashhad, Iran
author
Maryam
Mojdekanloo
North Khorasan University of Medical Sciences, Bojnurd, Iran
author
Zahra
Tayarani Najjaran
Pharmacological Research Centre of Medicinal Plants, School of Medicine, Mashhad, University of Medical Sciences, Mashhad, Iran
author
text
article
2016
eng
Objective: Biebersteinia (Geraniaceae) has a history of use in traditional medicine in some countries including Iran. In the present study, cytotoxic and apoptogenic properties of hydro-ethanol extract of B. multifidi was investigated on human prostate cancer cell lines (PC3 and DU 145) and human embryonic kidney 293 (HEK293) cells. Materials and Methods: Cells were cultured in RPMI-1640 medium supplemented with 10% FBS at 37ºC in a humidified atmosphere of 95% air and 5% CO2. The root of the plant was macerated with EtOH 70%. Cytotoxic activity of ethanol extract of B. multifida was assessed using alamarBlue® assay after 48 hr of treatment. Apoptotic cells were stained with propidium iodide (PI) and detected by flow cytometry (sub-G1 peak). Results: B. multifidi had cytotoxic effect on malignant cells and normal HEK293 cells in a dose-dependent manner and significantly decreased the cell viability (IC50 values were between 199.2 and 302.9 µg/ml). B. multifida increased the sub-G1 peak in flow cytometry histogram of treated PC3 cells compared to control showing the induction of apoptosis and DNA fragmentation. Conclusion: Due to cytotoxic and apoptotic activity of B. multifida, the plant is suggested for further phytochemical analysis and mechanistic evaluation.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
6
v.
6
no.
2016
671
677
https://ajp.mums.ac.ir/article_6793_0d35b9c7b7af1943a1d1e65d2eae4ed8.pdf
dx.doi.org/10.22038/ajp.2016.6793
Origanum vulgare leaf extract protects mice bone marrow cells against ionizing radiation
Reza
Ghasemnezhad Targhi
Department of Radiology, School of Allied, Tehran University of Medical Sciences, Tehran, Iran
author
Vahid
Changizi
Department of Radiology, School of Allied, Tehran University of Medical Sciences, Tehran, Iran
author
Farhang
Haddad
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
author
Mansour
Homayoun
Department of Anatomy, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Shokohozaman
Soleymanifard
Department of Medical Physic, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2016
eng
Objective: Ionizing radiation produces free radicals which induce DNA damage and cell death. Origanum vulgare leaf extract (OVLE) is a natural compound and its capability of scavenging free radicals and its antioxidant activity have been demonstrated by many researchers. In this study, using micronucleus assay, radioprotective effect of OVLE against clastogenic and cytotoxic effect of gamma irradiation has been investigated in mice bone marrow cells. Materials and Methods: OVLE was injected intraperitoneally to the BALB/c mice 1hr prior to gamma irradiation (3Gy) at the doses of 100 and 200 mg/kg. Twenty four hours after irradiation or treatment, animals were killed and smears were prepared from the bone marrow cells. The slides were stained with May Grunwald–Giemsa method and analyzed microscopically. The frequency of micronucleated polychromatic erythrocytes (MnPCEs), micronucleated normochromatic erythrocyte (MnNCEs) and cell proliferation ratio PCE/PCE+NCE (polychromatic erythrocyte/polychromatic erythrocyte + normochromatic erythrocyte) were calculated. Results: The results showed that gamma irradiation (3Gy) increased the frequency of MnPCEs, MnNCEs and reduced the PCE/PCE+NCE ratio in mice bone marrow compared to the non-irradiated control group (p< 0.0001). Injection of OVLE significantly reduced the frequency of MnPCEs (p< 0.0001) and MnNCEs (p< 0.05) and increased the PCE/PCE+NCE ratio as compared to the irradiated control group (p< 0.05). Conclusion: It seems that OVLE with its antioxidant properties and its capability of scavenging free radicals and reactive oxygen species can reduce the cytotoxic effects of gamma irradiation in mice bone marrow cells.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
6
v.
6
no.
2016
678
685
https://ajp.mums.ac.ir/article_6896_9e46888afb31b1f232a12d64a2d20e87.pdf
dx.doi.org/10.22038/ajp.2016.6896
Effects of Urtica dioica supplementation on blood lipids, hepatic enzymes and nitric oxide levels in type 2 diabetic patients: A double blind, randomized clinical trial
Alidad
Amiri Behzadi
Young Researcher and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran
author
Hamid
Kalalian-Moghaddam
Department of Physiology, Shahroud University of Medical Sciences, Shahroud, Iran
author
Amirhossein
Ahmadi
Department of Basic Sciences, Islamic Azad University Damghan Branch, Damghan, Iran
author
text
article
2016
eng
Objective: Oxidative stress plays an important role in the development of diabetic complications including metabolic abnormality-induced diabetic micro-vascular and macro-vascular complications. Urtica dioica L. (U. dioica) has been traditionally used in Iranian medicine as an herbal remedy for hypoglycemic or due to its anti-inflammatory properties. The aim of the present study was to evaluate the effects of hydro-alcoholic extract of U. dioica on blood lipids, hepatic enzymes and nitric oxide levels in patients with type 2 diabetes mellitus. Materials and Methods: 50 women with type 2 diabetes participated in this study and were randomly divided into two groups namely, control and intervention groups. Control group received placebo and intervention group received hydro-alcoholic extract of U. dioica. Before and after 8 weeks of continuous treatment, some biochemical serum levels including FPG, TG, SGPT, SGOT, HDL, LDL, SOD and NO were measured. Results: The results indicated that after 8 weeks, in the intervention group, FPG, TG, and SGPT levels significantly decreased and HDL, NO and SOD levels significantly increased as compared to the control group. Conclusion: Our results encourage the use of hydro-alcoholic extract of U. dioica as an antioxidant agent for additional therapy of diabetes as hydro-alcoholic extract of U. dioica may decrease risk factors of cardiovascular incidence and other complications in patients with diabetes mellitus.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
6
v.
6
no.
2016
686
695
https://ajp.mums.ac.ir/article_6763_4c182f71cfba204aa3bd97dbd28bcc18.pdf
dx.doi.org/10.22038/ajp.2016.6763
Effects of Mimosa pudica L. leaves extract on anxiety, depression and memory
Ganesh
Patro
School of Pharmaceutical Education & Research, Berhampur University, Bhanja Bihar, Berhampur-760007, Odisha, India
author
Himanshu
Sahoo
Department of Pharmacology, Roland Institute of Pharmaceutical Sciences, Berhampur-760010, Odisha, India
author
Bijay
Kumar Mohanty
Department of Botany & Biotechnology, Khallikote Autonomous College, Berhampur-760001, Odisha, India
author
text
article
2016
eng
Objective: The present study was carried out to investigate the neuropharmacological activities of ethyl acetate extract of Mimosa pudica (EAMP) leaves on anxiety, depression and memory in a mouse model. Materials and Methods: Anti-anxiety potential of EAMP was evaluated by elevated plus maze (EPM), light-dark box (LDB) and social interaction (SI) tests in mice.Anti-depressant potential of EAMP was evaluated by forced swimming (FST), tail suspension (TST), and open field tests (OFT). The behavioral findings were further corroborated with estimation of neurotransmitters and their metabolites from mouse brain homogenate. Effect on learning and memory was evaluated by EPM, passive avoidance (PA) tests. Further, it was confirmed with assessment of acetylcholinesterase and caspase-3 activity in brain homogenate. Results: EAMP showed significant anti-anxiety activity by increasing the time spent in open arm of EPM, light box of LDB. Social interaction time was increased significantly (p<0.01) as compared to vehicle control. There was also significant reduction of immobility time in both FST and TST without any changes in locomotor activity in the OFT. Monoamine neurotransmitters (dopamine and norepinephrine) concentrations were increased significantly (p<0.01) after 4 weeks of treatment as compared to stress control and substantiated the anti-depressant activity. Step down latency was increased (p<0.01) in PA test and transfer latency was decreased (p<0.01) in EPM test of EAMP-treated mice. Acetylcholinesterase and caspase-3 activity was significantly (p<0.05) changed in mice treated with EAMP (200 and 400 mg/kg). Conclusion: The results revealed that EAMP has anti-anxiety, anti-depressant and memory enhancing activities that are mediated through multiple mechanisms.
Avicenna Journal of Phytomedicine
Mashhad University of Medical Sciences
2228-7930
6
v.
6
no.
2016
696
710
https://ajp.mums.ac.ir/article_6895_88f734936dfeb869e7a8a9758020e064.pdf
dx.doi.org/10.22038/ajp.2016.6895